• Journal of Dairy Science and Biotechnology
• /
• 제28권2호
• /
• pp.31-39
• /
• 2010

Lactic acid bacteria (LAB) are typical probiotic microbes which are used in various industries including fermented foods, feed additives, and pharmaceuticals. The purpose of this study was to compare the ability of antioxidative activities of 23 strains of LAB isolated from cheese, kimchi, yogurt, and etc. LAB were tested for antioxidative activities such as 2,2'- azino-bis(3-ethyl-benzothiazoline-6-sulphonic acid) (ABTS) radical scavenging abilities and superoxide dismutase (SOD)-like ability. Antioxidative activities were examined different media such as MRS, M17, and 10% skim milk. Culture supernatant in M17 medium showed a higher antioxidative activity than these in MRS and 10% skim milk. Comparison of ABTS radical scavenging ability by the LAB showed a wide variation with a range from 9~93% and SOD-like ability of LAB ranged from 70~78%, respectively. Therfore, some of LAB can play important roles in the protection for oxidative stress and CPP-producing activity of LAB may be a promising material for application in the dairy industry.

• 약학회지
• /
• 제53권4호
• /
• pp.189-193
• /
• 2009

To investigate the antioxidant effect of lutein on N-nitrosodimethylamine (NDEA)-induced oxidative stress in mice, we measured lipid peroxidation, superoxide dismutase (SOD) and catalase of various tissues. Body weight was almost similar in lutein and control groups during 3 weeks. NDEA increased significantly the activities of typical marker enzymes of liver function (AST, ALT and ALP) in both groups. However, the increase of plasma aminotransferase activity significantly decreased in lutein group. Lipid peroxidation and SOD in various tissues, such as heart, lung, liver, kidney, spleen and plasma were significantly increased by NDEA, which were significantly reduced by lutein at a dose of 50 mg/kg. Catalase activity decreased significantly in control and lutein groups treated with NDEA, the effect being less in lutein group. Lesser effect on SOD and catalase in NDEA-treated lutein group indicates the improvement of protective mechanisms by lutein. Thus, it can be concluded from the present study that lutein can offer a useful protection against NDEA-induced oxidative stress.

• 한국자원식물학회지
• /
• 제21권6호
• /
• pp.457-461
• /
• 2008

Effects of Gal geun (Puerariae Radix) EtOH ext. on lipid lowering and antioxidant were investigated in hyperlipidemic rat. Concentration of FFA and triglyceride in plasma showed a tendency to decrease in Gal geun ext. groups. Concentration of plasma total cholesterol and LDL-cholesterol decreased in Gal geun ext. groups. However the concentration of HDL-cholesterol showed no significantly different in all treatment groups. Concentration of liver total cholesterol and triglyceride showed a tendence to decrease in Gal geun ext. groups. Concentration of plasma and liver TBARS showed a low values in Gal geun ext. groups. The values of GSH-Px and SOD activity showed no significantly different among all the treatment groups. However the values of SOD and CAT activity showed a high value in the Gal geun ext. groups.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권2호
• /
• pp.255-258
• /
• 2005

Several commercial yogurt starter cultures (L. acidophilus LA5, L. casei 01, L. acidophilus LA100, L. bulgaricus LB207 and L. rhamnosus GG744) were investigated for antioxidant activity by using in vitro assays. From the results of the present work, all strains tested showed varying degrees of antioxidant activity. L. bulgaricus LB207 showed the highest antioxidant activity, with a linoleic acid peroxidation inhibition of 81.3%. Hydroxy radical scavenging activity, ferrous iron chelating activity, reducing power and superoxide dismutase (SOD) activity were also studied. L. bulgaricus LB207 showed the highest hydroxy radical scavenging activity and L. casei 01 showed the highest chelating activity. L. bulgaricus LB207 and L. acidophilus LA100 showed good reducing power. All the strains in this study showed low SOD activity. The results of the present work suggest that antioxidant activity of L. bulgaricus LB207 due to its strong hydroxy radical scavenging activity and reducing power.

• 한국식품영양학회지
• /
• 제35권5호
• /
• pp.313-323
• /
• 2022

To investigate antioxidant effects of tea tree root extracts using various extraction methods, cytotoxicity, DPPH and ABTS radical scavenging, SOD, nitrite scavenging activity and inhibitory activity of lipid peroxidation, reducing power, ferrous ion chelating activity were measured. Cytotoxicity for RAW 264.7 cells was not observed at concentrations treated with below 90 ㎍/mL in all extracts. The maximum DPPH radical, nitrite scavenging, SOD activity and inhibitory activity of lipid peroxidation were obtained at the ethylacetate and 70% ethanol extract. The maximum ABTS radical scavenging activity was obtained at the ethylacetate and hot water extract. However, in the case of reducing power and ferrous ion chelating activity, they were obtained at 70% ethanol and hexane extract, respectively. Nitrate scavenging activity showed the most excellent scavenging ability of 59.6% at 90 ㎍/mL of ethylacetate. The hexane extract had the highest ferrous ion chelating activity, showing 61.05% at 50 ㎍/mL, 66.07% at 70 ㎍/mL and 76.81% at 90 ㎍/mL, respectively. The results of this research show that the ethylacetate and 70% ethanol extracts of tea tree root can be used as a natural material for scavenging the radicals. However, future study is necessary to understand the mechanism of antioxidant activity by identification of substances.

• Radiation Oncology Journal
• /
• 제28권2호
• /
• pp.91-98
• /
• 2010

목 적: Troglitazone (TRO)은 PPAR-$\gamma$ 작동제로서 온열감수성의 결정에 중요한 요인인 heat shock protein (HSP) 70의 합성을 저해하고 superoxide dismutase (SOD)와 카타라제를 증가시키는 것으로 알려져 있다. 이에 자궁경부암 세포를 대상으로 TRO가 온열감수성에 미치는 영향을 연구하였다. 대상 및 방법: HeLa 세포를 $5{\mu}M$ TRO로 24시간 처치한 후 $42^{\circ}C$에서 1시간 동안 온열처리를 시행하였다. 세포 생존 분획은 clonogenic assay로 측정하였다. 단백질 발현의 변화는 Western blot으로 분석하였다. SOD와 카타라제의 활성도를 측정하였으며, reactive oxygen species (ROS) 는 2',7'-dichlorofluorescin diacetate와 dihydroethidium 를 사용하여 측정하였다. 결 과: 온열처리에 의해 감소된 생존분획이 TRO 전처치에 의해 증가하였다. 온열처리에의해 HSP 70의 발현은 증가하였으나 TRO 전 처치에 의해 감소되지는 않았다. SOD와 카타라제의 활성도가 각각 1.2배, 1.3배 증가하였다. 온열처리에 의해 ROS가 증가하였으며, 증가된 ROS는 TRO 전 처치에 의해 감소하였다. 결 론: TRO는 SOD와 카타라제의 활성도를 증가시키며 이는 온열에 의한 ROS를 감소시켜 결과적으로 온열감수성을 저하시킨다.

• Journal of Acupuncture Research
• /
• 제19권3호
• /
• pp.115-125
• /
• 2002

Objective : This dissertation was designed to evaluate the effect of BCL(refinded Bambusae Caulis in Liqua-men) oral administration and herbal acupuncture on alcohol metabolism and liver function. Methods : Mice were damaged by a large quantity of alcohol and received treatment of either BCL $1mg/kg$ in oral or BCL $250{\mu}g/kg$ in herbal acupuncture-BL18 BL19 bilateral. and then such parameters as GOT, GPT, catalase and superoxide dismustase(CuZn-SOD, Mn-SOD) were measured. Results : 1. Compared with control group, the activity of GOT, GPT in serum was significantly reduced and the proper degree of alcohol in serum was not significantly differ from oral administration group and herbal acupuncture group. 2. The activity of catalase in liver cell tissue and the activity of CuZn-SOD in liver cell tissue, compared with control group, was not sigificantly affected either by oral administration and herbal acupuncture group. But the activity of Mn-SOD was significantly increased in oral administration group, while it was not the case in acupuncture group. Conclusion : we consider that BCL oral administration and herbal acupuncture is highly effective in recovering alcohol metabolism and liver disfunction induced by alcohol.

• Journal of Nutrition and Health
• /
• 제35권1호
• /
• pp.30-36
• /
• 2002

This study was conducted to evaluate the effect of regular exercise and other relating factors on the activities of erythrocyte antioxidant enzymes and plasma total radical-trapping antioxidant potential (TRAP) in 61 healthy male college students. The study population were divided in two groups ; small amount of exerciser (exorcise time less than 30min/d) and moderate amount of exerciser (exorcise time more than 30min/d) according to their physical exercise habits measured by a questionnaire. Dietary intake of vitamin C and vitamin E, Plasma lipid Profiles, erythrocyte superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and catalase activities, as well as plasma TRAP levels were determined. Plasma TRAP level was significantly higher in moderate amount of exercisers than that in small amount of exercisers. No significant differences were observed in erythrocryte SOD, catalase and GSH-Px between the two groups. Mean exercise time was positively correlated with the plasma level of TRAP significantly, and amount of alcohol consumption was negatively correlated with the erythrocyte SOD activity, Dietary vitamin C and I intakes did not correlated with either erythrocyte enzyme activities or plasma TRAP levels. There were positive correlations between plasma HDL-cholesterol, and erythrocyte GSH-Px or plasma TRAP levels. Plasma vitamin C concentrations was negatively correlated with plasma TRAP levels and erythrocyte SOD activity, however plasma vitamin C concentration was positively correlated with erythrocyte GSH-Px activity, The results would suggest that regular moderate exercise, nonsmoking, high HDL-cholesterol and high plasma vitamin E concentration enhance antioxidant defences against reactive oxygen species and may increase the likelihood of a healthier life span.

• Toxicological Research
• /
• 제17권1호
• /
• pp.41-47
• /
• 2001

In order to test the effects of hyperbaric oxygen (HBO) and $\alpha$-tocopherol on full-thickness skin grafts in rats, we peeformed full-thickness skin grafts bilaterally on rats. After surgery, we analyzed the tissue-concentrations of superoxide dismutase (SOD), catalase, and glutathione peroxidase(GPx)/reductase(GPr) on days 0, 2, 4, 7, 10, 14, 21 and 28. The four groups had similar patterns of change in SOD, catalase, GPx and GPr values. SOD increased initially, and was significantly increased at day 7, returning to the preoperative activity level on day 14 (control, HBO, and $\alpha$-tocopherol treated alone) and 28 (HBO plus $\alpha$-tocopherol). Catalase had a similar pattern of change as the SOD enzyme activity, except for the surgical control on day 2. Glutathione peroxidase/reductase activity in the four groups had a similar pat-tern of enzyme activity, with a significant increase from preoperative level on day 4, peaking during days 7 to 10, and returning to preoperative level on day 21(surgical control, HBO, and $\alpha$-tocopherol-treated alone) and 28 (HBO plus $\alpha$-tocopherol treated group). Hence, the clinical use of HBO and $\alpha$-tocopherol mixture can be recommended as an adjunctive treatment for free skin grafts in rats. But, the antioxidant used, its dose, and the timing of its administration, as well as, the exposure time and the pressure of HBO, should be the subject of further research.

• 대한한의학방제학회지
• /
• 제29권1호
• /
• pp.1-8
• /
• 2021

Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Ojayeonjonghwan (OYH) in GC-1 germ cell lines. Methods : DPPH radical scavenging activity and cell viability assays in GC-1 germ cell lines were performed. In addition, the protective effects of OYH against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines were examined by measuring cell viability after H2O2 treatmet. The formation of ROS and the antioxidant enzymes activity such as SOD and catalase were measured in the same condition. Results : OYH scavenged DPPH radical dose-dependent manner and the IC50 was 63.79 ㎍/ml. OYH showed no cytotoxicity at concentration of 1, 10, 100 ㎍/ml. The hydrogen peroxide-induced cytotoxicity of GC-1 germ cell lines was protected to 53.66% by OYH at concentration of 10 ㎍/ml. OYH effectively inhibited ROS production in GC-1 germ cell lines. Mn SOD and catalase protein expression were significantly increased in GC-1 germ cell lines, but Cu/Zn SOD protein expression was not significantly changed. Conclusions : In conclusion, OYH has antioxidant activities against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines.