• Title/Summary/Keyword: SLIDE 2D

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Growth of Thin Film using Chemical Bath Deposition Method and Their Photoconductive Characterics ($Cd_{1-x}Zn_{x}S$ 박막의 성장과 광전도 특성)

  • Lee, S.Y.;Hong, K.J.;You, S.H.;Shin, Y.J.;Lee, K.K.;Suh, S.S.;Kim, H.S.;Yun, E.H.;Kim, S.U.;Park, H.S.;Shin, Y.J.;Jeong, T.S.;Shin, H.K.;Kim, T.S.;Moon, J.D.;Lee, C.I.;Jeon, S.L.
    • Journal of Sensor Science and Technology
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    • v.4 no.3
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    • pp.60-70
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    • 1995
  • Polycrystalline $Cd_{1-x}Zn_{x}S$ thin film were grown on slide glass(corning-2948) substrate using a chemical bath deposition (C.B.D) method. They were annealed at various temperature and X -ray diffraction patterns were measured by X-ray diffractometor in order to study $Cd_{1-x}Zn_{x}S$ polycrystal structure using extrapolation method of X-ray diffraction patterns for the CdS, ZnS sample annealed in $N_{2}$ gas at $550^{\circ}C$. It was found hexagonal structure which had the lattice constant $a_{0}\;=\;4.1364{\AA}$, $c_{0}\;=\;6.7129{\AA}$ in CdS and $a_{0}\;=\;3.8062{\AA}$, $c_{0}\;=\;6.2681{\AA}$ in ZnS, respectively. Hall effect on these sample was measured by Van der Pauw method and then studied on carrier density and mobility depending on temperature. We measured also spectral response, sensitivity maximum allowable power dissipation and response time on these sample.

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Development of Mono Layer Cell Adsorption Apparatus to Create a Slide for Microscopic Diagnosis (현미경 진단용 슬라이드 제작을 위한 단층세포 흡착장치 개발)

  • Oh, H.Y.;Mun, M.G.;Kim, S.H.;Kim, D.W.;Kang, S.M.;Sung, R.G.;Kim, H.C.
    • Journal of Biomedical Engineering Research
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    • v.36 no.1
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    • pp.1-6
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    • 2015
  • This study aims to design a monolayer cell adsorption apparatus that would help to produce high-quality slides for Liquid-Based Cytology (LBC) of an early cancer diagnosis for human bodies. The LBC collects exfoliated cells of human bodies and spreads the cells on the slides. Through processes of dyeing and cytological examination, the LBC screens for cancers in early stage. In this study, both of a cell suction module and a cell adsorption module, which are the key elements of the monolayer cell adsorption apparatus, were developed, and using those modules, the study set, first, conditions to help both GYN and NON-GYN apply principal cells without de-endothelialization before conducting its own analysis on the utility. As a results, for GYN, apparatus was determined to be able to produce high-quality slides under the condition of 4 and for NON-GYN, the apparatus would come up with other slides of high-quality under the condition of 2. The study carried out a repetitive test on selected conditions and proved 96% of the repetitive success rate. By the results of what has been learned so far, the study presents that the apparatus has a possibility to replace device from South Korea as one of those other currently-applied systems to run the LBC and that the system will also present a new paradigm for cancer diagnosis as it makes a contribution to the improvement in the LBC.

Optimal Conditions of Single Cell Gel Electrophoresis (Comet) Assay to detect DNA single strand breaks in Mouse Lymphoma L5178Y cells

  • Ryu, Jae-Chun;Kwon, Oh-Seung;Kim, Hyung-Tae
    • Environmental Mutagens and Carcinogens
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    • v.21 no.2
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    • pp.89-94
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    • 2001
  • Recently, single cell gel electrophoresis, also known as comet assay, is widely used for the detection and measurement of DNA strand breaks in vitro and in vivo in many toxicological fields such as radiation exposure, human monitoring and toxicity evaluation. As well defined, comet assay is a sensitive, rapid and visual method for the detection of DNA strand breaks in individual cells. Briefly, a small number of damaged cells suspended in a thin agarose gel on a microscope slide were lysed, unwinded, electrophoresed, and stained with a fluorescent DNA binding dye. The electric current pulled the charged DNA from the nucleus such that relaxed and broken DNA fragments migrated further. The resulting images which were subsequently named for their appearance as comets, were measured to determine the extent of DNA damages. However, some variations could be occurred in procedures, laboratories's conditions and kind of cells used. Hence, to overcome and to harmonize these matters in comet assay, International Workshop on Genotoxicity Test Procedure (IWGTP) was held with several topics including comet assay at Washington D.C. on March, 1999. In spite of some consensus in procedures and conditions in IWGTP, there are some problems still remained to be solved. In this respect, we attempted to set the practical optimal conditions in the experimental procedures such as lysis, unwinding, electrophoresis and neutralization conditions and so on. First of all, we determined optimal lysis and unwinding time by using 150 $\mu$M methyl methanesulfonate (MMS) which is usually used concentration. And then, we determined optimal positive control concentrations of benzo(a)pyrene (BaP) and MMS in the presence and absence of S9 metabolic activation system, respectively.

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Stability assessment of soil slopes in three dimensions: The effect of the width of failure and of tension crack

  • Pantelidis, Lysandros;Gravanis, Elias;Gkotsis, Konstantinos-Paraskevas
    • Geomechanics and Engineering
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    • v.22 no.4
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    • pp.319-328
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    • 2020
  • This paper investigates the effect of the width of failure and tension crack (TC) on the stability of cohesive-frictional soil slopes in three dimensions. Working analytically, the slip surface and the tension crack are considered to have spheroid and cylindrical shape respectively, although the case of tension crack having planar, vertical surface is also discussed; the latter was found to return higher safety factor values. Because at the initiation of a purely rotational slide along a spheroid surface no shear forces develop inside the failure mass, the rigid body concept is conveniently used; in this respect, the validity of the rigid body concept is discussed, whilst it is supported by comparison examples. Stability tables are given for fully drained and fully saturated slopes without TC, with non-filled TC as well as with fully-filled TC. Among the main findings is that, the width of failure corresponding to the minimum safety factor value is not always infinite, but it is affected by the triggering factor for failure (e.g., water acting as pore pressures and/or as hydrostatic force in the TC). More specifically, it was found that, when a slope is near its limit equilibrium and under the influence of a triggering factor, the minimum safety factor value corresponds to a near spherical failure mechanism, even if the triggering factor (e.g., pore-water pressures) acts uniformly along the third dimension. Moreover, it was found that, the effect of tension crack is much greater when the stability of slopes is studied in three dimensions; indeed, safety factor values comparable to the 2D case are obtained.

Application of Remote Sensing and Geographic Information System in Forest Sector (원격탐사와 지리정보시스템의 산림분야 활용)

  • Lee, Woo-Kyun;Kim, Moonil;Song, Cholho;Lee, Sle-gee;Cha, Sungeun;Kim, GangSun
    • Journal of Cadastre & Land InformatiX
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    • v.46 no.2
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    • pp.27-42
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    • 2016
  • Forest accounts for almost 64 percents of total land cover in South Korea. For inventorying, monitoring, and managing such large area of forest, application of remote sensing and geographic information system (RS/GIS) technology is essential. On the basis of spectral characteristics of satellite imagery, forest cover and tree species can be classified, and forest cover map can be prepared. Using three dimensional data of LiDAR(Light Detection and Ranging), tree location and tree height can be measured, and biomass and carbon stocks can be also estimated. In addition, many indices can be extracted using reflection characteristics of land cover. For example, the level of vegetation vitality and forest degradation can be analyzed with VI (vegetation Index) and TGSI (Top Grain Soil Index), respectively. Also, pine wilt disease and o ak w ilt d isease c an b e e arly detected and controled through understanding of change in vegetation indices. RS and GIS take an important role in assessing carbon storage in climate change related projects such as A/R CDM, REDD+ as well. In the field of climate change adaptation, impact and vulnerability can be spatio-temporally assessed for national and local level with the help of spatio-temporal data of GIS. Forest growth, tree mortality, land slide, forest fire can be spatio-temporally estimated using the models in which spatio-temporal data of GIS are added as influence variables.

A Case Study on the Slope Collapse and Reinforcement Method of the Phyllite Slope (천매암 지역에서의 비탈면 붕괴 원인규명 및 보강대책 사례연구)

  • Cho, Younghun;Lim, Daesung;Chun, Byungsik
    • Journal of the Korean GEO-environmental Society
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    • v.11 no.8
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    • pp.83-93
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    • 2010
  • The purpose of this study is to present emergency rehabilitation, cause and the countermeasure of reinforcement about reinforced retaining wall and the slope collapse of the phyllite ground. The study area is broken easily because this area has rock mass discontinuity such as stratification, foliation, joint and fold. And this area consists of the ground where it happens easily to the failure of structure like reinforced retaining wall because of the phyllite ground sensitive to weathering. Counterweight fill in front of reinforced retaining wall was performed as emergency rehabilitation about displacement of reinforced retaining wall and the failure at the rear of slope on phyllite ground. After that, additional displacement didn't occur. Boring and geophysical exploration were launched to present emergency rehabilitation and develop the long-term method of reinforcement. This could grasp anticipated range of the failure section and identify internal and external factors of the cause of the slope collapse. Several methods of reinforcement were suggested by conducting the numerical analysis. When conducting design and construction of major structures at the ground which has complex discontinuities, the precise site investigation should be conducted. During construction, immediate action for over-displacement should be taken by performing the periodic measurement.

Studies on Chemical Resistance of Mites. II. Orchard Mite Control and their Resistance to Metasystox, Folidol and C-8514 in Korea (응애류의 약제 저항성에 관한 연구 II . Metasystox, Folidol 및 C-8514에 대한 과수응애류의 저항성과 방제시험)

  • Lee S. C.;Yoo J. K.
    • Korean journal of applied entomology
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    • v.10 no.2
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    • pp.109-116
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    • 1971
  • The study involved determination of resistance levels of spider mites to Metasystox, Folidol and C-8514 using slide dip technique: a feid trial of alternative acaricides using an O/P resistant strain. 1. Resistant strains of two-spotted spider mite( Tetranychus urticae Koch) were collected from Taegu, Kyungsan, Chunchun, Yesan, Suwon, Osan and Chungju, and kept in the glasshouse through the experiments. Resistant strains of European red mite (Panonychus ulmi Koch) were collected from Chungju, Taegu, Yesan, Chunchun, Suwon and Whasung, and Wonju susceptible was collected. Immediately after collection, tests were applied. All strains except susceptible populations had regularly been sprayed with Organo-phosphates including Parathion (or Folidol), Metasystox, Phenkapton and Trithion for more than the ten Bast years; and Organo-chlorines such as Kelthane and C-8514 more than five years. Comparisons of the resistant strains and susceptible strains at the $LC_50$ values are as follows: a. With Metasystox, resistance levels of the Chungju, Taegu, Yesan, Chunchun and Suwon resistant strains of P. ulmi were respectively, 96,52,4,3 and 2, times as resistant as the Wonju susceptible strain. b. With Folidol, resistance levels of the Chungju, Chunchun, Yesan, Whasung and Suwon strains of P. ulmi showed respectively, 126, 48, 33, 30 and 6-fold resistance levels over the susceptible strain. c. With C-8514, resistance levels of the Taegu, Suwon, Whasung and Chungju strains of P. ulmi were 42, 31, 30 and 20 times greater than the susceptible strain, respectively. d. With Metasystox, resistance levels of the Taegu, Chunchun, Yesan and Suwon, strains of T urticae were respectively, 32,29,25 and 17 times as resistant as the susceptible strain. e. With C-8514, resistance levels of the Taegu, Chungju and Kyungsan strains of T. urticae showed respectively, 59, 29 and 19-fold resistance levels over the Osan strain. 2. Field trials were carried out to assess the toxicities of eleven alternative materials which would be used for control of O/P resistant strain of Panonychus ulmi. The acaricide groups represented were 2 Organo-chlorines (Kelthane MF and Prethylen), 1 carbamate (Furadan), 1 nitrophenyl (Morocide), 1 carbonate (Morestan), 1 tin(Plictran), 1 fluorine (Nissol), 2 mixtures (Fundal and Banmite) and Organo-phosphate (Phenkapton). Of all acaricides tested Kelthane, Plictran, Fundal and Morestan were the most effective; fol]owed by Banmite, Furadan, Prethylen and Nissol. Sumite and Morocide were intermediate, but Phenkapton(Organo-phosphate) was very poor. In other words, a first application of the above outstanding materials gave very high degrees of control of O/P resistant population of European red mite for about 7 to 12 days. However, the results indicate that secondary application would sometimes be necessary. There was no foliage damage to apple trees by any of the acaricides tested of the concentrations used.

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Studies on the Mechanical Properties of Weathered Granitic Soil -On the Elements of Shear Strength and Hardness- (화강암질풍화토(花崗岩質風化土)의 역학적(力學的) 성질(性質)에 관(關)한 연구(硏究) -전단강도(剪斷强度)의 영향요소(影響要素)와 견밀도(堅密度)에 대(對)하여-)

  • Cho, Hi Doo
    • Journal of Korean Society of Forest Science
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    • v.66 no.1
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    • pp.16-36
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    • 1984
  • It is very important in forestry to study the shear strength of weathered granitic soil, because the soil covers 66% of our country, and because the majority of land slides have been occured in the soil. In general, the causes of land slide can be classified both the external and internal factors. The external factors are known as vegetations, geography and climate, but internal factors are known as engineering properties originated from parent rocks and weathering. Soil engineering properties are controlled by the skeleton structure, texture, consistency, cohesion, permeability, water content, mineral components, porosity and density etc. of soils. And the effects of these internal factors on sliding down summarize as resistance, shear strength, against silding of soil mass. Shear strength basically depends upon effective stress, kinds of soils, density (void ratio), water content, the structure and arrangement of soil particles, among the properties. But these elements of shear strength work not all alone, but together. The purpose of this thesis is to clarify the characteristics of shear strength and the related elements, such as water content ($w_o$), void ratio($e_o$), dry density (${\gamma}_d$) and specific gravity ($G_s$), and the interrelationship among related elements in order to decide the dominant element chiefly influencing on shear strength in natural/undisturbed state of weathered granitic soil, in addition to the characteristics of soil hardness of weathered granitic soil and root distribution of Pinus rigida Mill and Pinus rigida ${\times}$ taeda planted in erosion-controlled lands. For the characteristics of shear strength of weathered granitic soil and the related elements of shear strength, three sites were selected from Kwangju district. The outlines of sampling sites in the district were: average specific gravity, 2.63 ~ 2.79; average natural water content, 24.3 ~ 28.3%; average dry density, $1.31{\sim}1.43g/cm^3$, average void ratio, 0.93 ~ 1.001 ; cohesion, $ 0.2{\sim}0.75kg/cm^2$ ; angle of internal friction, $29^{\circ}{\sim}45^{\circ}$ ; soil texture, SL. The shear strength of the soil in different sites was measured by a direct shear apparatus (type B; shear box size, $62.5{\times}20mm$; ${\sigma}$, $1.434kg/cm^2$; speed, 1/100mm/min.). For the related element analyses, water content was moderated through a series of drainage experiments with 4 levels of drainage period, specific gravity was measured by KS F 308, analysis of particle size distribution, by KS F 2302 and soil samples were dried at $110{\pm}5^{\circ}C$ for more than 12 hours in dry oven. Soil hardness represents physical properties, such as particle size distribution, porosity, bulk density and water content of soil, and test of the hardness by soil hardness tester is the simplest approach and totally indicative method to grasp the mechanical properties of soil. It is important to understand the mechanical properties of soil as well as the chemical in order to realize the fundamental phenomena in the growth and the distribution of tree roots. The writer intended to study the correlation between the soil hardness and the distribution of tree roots of Pinus rigida Mill. planted in 1966 and Pinus rigida ${\times}$ taeda in 199 to 1960 in the denuded forest lands with and after several erosion control works. The soil texture of the sites investigated was SL originated from weathered granitic soil. The former is situated at Py$\ddot{o}$ngchangri, Ky$\ddot{o}$m-my$\ddot{o}$n, Kogs$\ddot{o}$ng-gun, Ch$\ddot{o}$llanam-do (3.63 ha; slope, $17^{\circ}{\sim}41^{\circ}$ soil depth, thin or medium; humidity, dry or optimum; height, 5.66/3.73 ~ 7.63 m; D.B.H., 9.7/8.00 ~ 12.00 cm) and the Latter at changun-long Kwangju-shi (3.50 ha; slope, $12^{\circ}{\sim}23^{\circ}$; soil depth, thin; humidity, dry; height, 10.47/7.3 ~ 12.79 m; D.B.H., 16.94/14.3 ~ 19.4 cm).The sampling areas were 24quadrats ($10m{\times}10m$) in the former area and 12 in the latter expanding from summit to foot. Each sampling trees for hardness test and investigation of root distribution were selected by purposive selection and soil profiles of these trees were made at the downward distance of 50 cm from the trees, at each quadrat. Soil layers of the profile were separated by the distance of 10 cm from the surface (layer I, II, ... ...). Soil hardness was measured with Yamanaka soil hardness tester and indicated as indicated soil hardness at the different soil layers. The distribution of tree root number per unit area in different soil depth was investigated, and the relationship between the soil hardness and the number of tree roots was discussed. The results obtained from the experiments are summarized as follows. 1. Analyses of simple relationship between shear strength and elements of shear strength, water content ($w_o$), void ratio ($e_o$), dry density (${\gamma}_d$) and specific gravity ($G_s$). 1) Negative correlation coefficients were recognized between shear strength and water content. and shear strength and void ratio. 2) Positive correlation coefficients were recognized between shear strength and dry density. 3) The correlation coefficients between shear strength and specific gravity were not significant. 2. Analyses of partial and multiple correlation coefficients between shear strength and the related elements: 1) From the analyses of the partial correlation coefficients among water content ($x_1$), void ratio ($x_2$), and dry density ($x_3$), the direct effect of the water content on shear strength was the highest, and effect on shear strength was in order of void ratio and dry density. Similar trend was recognized from the results of multiple correlation coefficient analyses. 2) Multiple linear regression equations derived from two independent variables, water content ($x_1$ and dry density ($x_2$) were found to be ineffective in estimating shear strength ($\hat{Y}$). However, the simple linear regression equations with an independent variable, water content (x) were highly efficient to estimate shear strength ($\hat{Y}$) with relatively high fitness. 3. A relationship between soil hardness and the distribution of root number: 1) The soil hardness increased proportionally to the soil depth. Negative correlation coefficients were recognized between indicated soil hardness and the number of tree roots in both plantations. 2) The majority of tree roots of Pinus rigida Mill and Pinus rigida ${\times}$ taeda planted in erosion-controlled lands distributed at 20 cm deep from the surface. 3) Simple linear regression equations were derived from indicated hardness (x) and the number of tree roots (Y) to estimate root numbers in both plantations.

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