• Title/Summary/Keyword: SIV

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Comparison of serological methods for detection of avian influenza virus antibodies (가금인플루엔자 바이러스 항체검출을 위한 혈청학적 진단법 비교)

  • Han, Myung-guk;Park, Kyoung-yoon;Kwon, Yong-kuk;Kim, Jae-hong
    • Korean Journal of Veterinary Research
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    • v.42 no.1
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    • pp.73-80
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    • 2002
  • An enzyme-linked immunosorbent assay (ELISA) using purified hemagglutinin of swine influenza virus (H1N1) as antigen was developed for detection of antibody to avian influenza virus (AIV). The sensitivity and specificity of a developed and commercial available ELISA kits were compared with those of agar gel precipitation (AGP) test and hemagglutination inhibition (HI) test using sera collected from chickens under condition of field exposure. The concentration of antigen, serum dilution and concentration of enzyme-conjugated secondary antibody in developed ELISA (S-ELISA) were 0.5ug/100ul, 1:200 and 0.03ug/100ul, respectively. The correlation coefficients between S-ELISA and commercial ELISA and HI titers were 0.419 and 0.533, respectively. A significant correlation (p < 0.01) was not found between HI and ELISA titers. The S-ELISA was found to be as more sensitive and specific than the AGP test, showing 86.8% sensitivity and 85.3% specificity. It is suggested that the ELISA using the SIV as antigen may be useful method as an investigating tool for AIV serological surveillance.

Applicability of Surface Image Velocimetry Using Far Infarared Cameras (원적외선 카메라를 이용한 표면영상유속계의 적용성 검토)

  • Kim, Sanghyuk;Yu, Kwonkyu;Bae, Inhyuk;An, Myeong-Hui;Yoon, Byungman
    • Proceedings of the Korea Water Resources Association Conference
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    • 2015.05a
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    • pp.71-71
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    • 2015
  • 표면영상유속계(Surface Image Velocimety, SIV)는 하천 표면의 영상을 분석하여 유속을 산정하는 매우 실용적이며 간편한 유속측정 방법이다. 그러나 표면영상유속계는 수표면의 움직임을 계산하여 표면유속을 산정하기 때문에 빛이 없는 야간의 경우 수표면의 움직임을 촬영하기 어려워 밤에 발생하는 홍수 유량 측정이 어려운 단점이 있다. 이를 해결하기 위해 수표면에 야광 입자를 흘려 촬영하는 방법이 있었으나 대하천의 경우 야광물질의 크기가 작아 영상 내에서 식별이 어려운 문제가 있었고, 높은 밝기의 조명을 사용하여 영상을 획득하는 방법 또한 수행되었으나 빛이 수표면에 반사되어 하천 전체를 촬영하기 어려운 문제점이 있었다. 이 후 근적외선 카메라를 이용하여 야간 측정의 어려움을 극복하고자 하였으나 촬영범위가 최대 50 m 정도로 조사 거리의 한계를 나타내었다. 최근 Fujita(2013)는 원적외선카메라를 이용한 하천 유량 측정을 실시하였는데 이는 기존 야간촬영 시 시도되었던 방법의 문제점을 해결할 수 있음을 보여주었다. 이에 본 연구에서는 원적외선 카메라를 이용한 표면영상유속계의 적용성을 검토하고자 실험수로에서 실험을 수행하였다. 실험 시 주간 및 야간의 상황을 설정하여 원적외선 카메라, 근적외선 카메라 그리고 일반 캠코더를 이용하여 흐름을 촬영하였다. 또한 프로펠러유속계를 이용하여 흐름의 표면유속을 측정하였다. 원적외선 카메라를 이용하여 촬영된 영상을 상호상관법을 이용하여 표면유속을 측정하였고, 이를 프로펠러유속계 측정결과와 비교하여 정확도를 검토하였다. 또한 일반 캠코더와 근적외선카메라의 영상 분석 결과와도 비교하여 개선점을 확인하였다.

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Instantaneous Voltage Control Scheme of Auxiliary Power Supply System for Electric Railway Vehicles (철도차량 보조전원장치의 순시전압제어)

  • 김재식;최재호;임성수;이은규
    • The Transactions of the Korean Institute of Power Electronics
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    • v.4 no.4
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    • pp.349-356
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    • 1999
  • This paper presents an instantaneous voltage control scheme of au킹liary power supply system for the electric railway v vehicles, The resonance problem of the LC filter and the existing steady state error are more serious as the use of l instantaneous voltage control techniques for the fast transient response at the nonlinear load, A filter capacitor current f feedback loop is considered to increase the damping ratio of the voltage transfer function for the suppression of the resonance problem of the LC inverter output filter. To eliminate the steady state en‘or existing in case of the AC l instantaneous voltage control. the high gain transfer function is added to the conventional PI controller. The theoretical a analysis is well described with the simulation results. The validity of the proposed schemes is well verified through the s simulation and expelimental results for the 5 kVA prototype.

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Study on values and value change in clinical nurses (임상간호사들의 가치관 및 가치성향에 대한 연구)

  • 이경혜;방희숙;왕임순
    • Journal of Korean Academy of Nursing
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    • v.25 no.2
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    • pp.279-294
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    • 1995
  • The purpose of this study was to determine values and value changes in clinical nurses in clinical situations. A survey was conducted of 200 nurses of one of university hospital in Seoul Korea. This study was conducted between July 1 and July 5, 1994, using the Survey of Interpersonal Values (SIV) developed by L. V. Gordon, and standardized for Korea by Kyung Hye Lee & Eung Yun Hwang. The results of the study were as follows, 1. The clinical nurses surveyed showed Independence(18.32 : 63rd percentile) as their highest value, with Support(14.8 : 62nd percentile) next and then Benevolence(16.28 53rd percentile), Leadership(9.06 : 46th percentile), Conformity(18.15 : 42nd percentile) and Recognition(18.32 : 41s1 percentile) in that order in the area of general values. Using a standardised women's value Indicator, the clinical nurses value orientation was within the 32nd∼69th percentile indicating means found among Korean women in general. 2. Looking at how the values were related to demographics, Leadership was most highly valued among the 30∼34 year olds and least valued among the 25∼29 year olds. Unmarried nurses valued Independence more than married nurses did, and junior college graduate nurses valued Conformity more than baccalaureate graduate nurses did. 3. The study showed that the values of with less than one year (16.00) clinical experience were higher than those who had over 10 years (13.60) clinical experience (p<.05). Therefore clinical experience did not positively influence value orientation for clinical nurses. 4. There was no significant differences in the value of nurses in relation to their workplace, their level of motivation, or their aptitude. The study shows that clinical nurses have similar values compared with ordinary Korean women. This means that professional nurses may not be able to satisfy client needs and also that they do not have satisfaction and a positive attitude regarding the nursing profession. Therefore it is suggested that new strategies and continuing education programs be established to help clinical nurses fomulate higher values.

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Sero-epidemiology of the major swine infectious disease in Cheju (제주지역에 대한 돼지 주요 전염병의 혈청학적 역학조사)

  • Lyoo, Young-soo;Park, Choi-kyu;Kim, Lo-mi;Lee, Chang-hee;Choi, Sang-ho;Kim, Sung-il;Bae, Jong-hee
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.765-772
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    • 1997
  • 제주지역 돼지에서 각종 전염성 질병 원인체에 대한 항체를 조사하여 그간 전염성 병원체에 대한 역학조사가 미진하였던 부분을 보완하여 질병의 분포를 파악하고자 1995년부터 1996년에 걸쳐 제주도 전역에서 돼지의 혈청을 채취하여 각종 병원체에 대한 항체 분포율을 조사하였다. 본 연구에서 검사한 돼지 혈청 시료에서는 돼지 오제스키병 바이러스에 대한 항체는 전혀 검출되지 않았다. 돼지 콜레라바이러스에 대한 항체는 기대 수준 이하로 낮아 백신접종이 원활히 수행되고 있지 않음을 시사하였으며 특히 농장에 따라 항체 보유돈과 항체 음성돈이 혼재하는 농장과 항체가 전혀 검출되지 않는 농장 등 돼지 콜레라 방역의 사각지대가 존재할 가능성이 있음을 보여주었다. 유 사산 원인체인 돼지 파보바이러스 및 뇌심근염에 대한 항체가가 다양하게 나타나 일부 문제가 있을 것으로 사료되었다. 돼지 생식기호흡기증후군(PRRS) 바이러스에 대한 항체 분포율은 내륙 보다 다소 낮게 나타났고, 돼지 influenza virus, 위축성 비염, 흉막 폐염 등 각종 세균성 질환에 대한 항체수준도 다양하게 나타났다. 본 혈청학적인 연구결과는 제주지역에서의 양돈방역 정책수립 및 질병방제의 기초자료로 유용하게 이용될 것으로 사료된다.

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Hormonic Evaluation of An Imported IAT(Intra Airport Transit) System in Incheon International Airport (인천 국제공항청사 경전철 도입에 따른 고조파 영향 분석)

  • Kim, Jin-O;Song, Hak-Seon
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.20 no.1
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    • pp.41-47
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    • 2006
  • This paper presents harmonic evaluation of an IAT(Intra Airport Transit) system in Incheon International Airport. It will be used for electric vehicles with 80[kW] per car produced by Mitsubishi Heavy Industries Ltd, and which is constructed with SIV(Static Inverter), VVVF controller and two induction motor. The vehicles operated in the IAT system can be treated as rapidly changing DC loat and at a feeding substation, 3-phase electric power is transferred to DC 750[V] by rectifier. Since vehicles are changing continuously, the voltages for the load fluctuate in the IAT system, and moreover, the voltage fluctuation generates high-order harmonics. It results the difficulty in maintaining power quality in KEPCO systems' side. The power quality of the IAT system in Incheon International Airport is evaluated using PSCAD/EMTDC simulator in the paper. The THD(Total Harmonic Distortion) of voltages and TDD(Total Demand Distortion) of currents, indices are calculated for the IAT system using the results of PSCAD/EMTDC dynamic simulation.

Seroprevalence survey of swine influenza virus (H1N1, H3N2) in pigs in Gyeongnam area (경남지역 내 돼지에서의 swine influenza virus (H1N1, H3N2) 감염률 조사)

  • Jang, Eun-Hee;Hah, Do-Yun;Park, Dong-Yeop;Lee, Kuk-Cheon;Heo, Jung-Ho
    • Korean Journal of Veterinary Service
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    • v.34 no.3
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    • pp.195-200
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    • 2011
  • Swine influenza is an acute respiratory disease prevalent in pig-growing areas all around the world and plays the roles of an intermediate host to be transmitted to mammals including human beings through a genetic recombination with the avian influenza virus. Recognizing that people could be contracted with swine influenza, this study set out to investigate the seroprevalence of individual and multiple infections with two subtypes (H1N1 and H3N2) of the swine influenza virus in pig farms in the Gyeongnam region according to age, area, and season, as well as to provide basic data for the prevention and control of swine influenza. Used in the study were total 904 swine sera that were not vaccinated against the influenza gathered from the pig farms in the Gyeongnam region from November, 2009 to October, 2010. HerdChek SIV (H1N1, H3N2) ELISA kit (IDEXX Laboratories, USA) was used for antibody testing against swine influenza. The test results show that 370 sera (40.9%) were infected with either H1N1 or H3N2 with 37.3% (337 sera) being contracted with H1N1, 13.1% (118 sera) with H3N2, and 9.4% (85) with both H1N1 and H3N2.

A study on the flow measurement method of river using High-performance GPS floater (고성능 GPS 전자부자를 활용한 하천 수리량 측정법 연구)

  • Lee, Jeong Min;Lee, Chang Hyun;Kim, Young Do;Kim, Dongsu
    • Proceedings of the Korea Water Resources Association Conference
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    • 2021.06a
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    • pp.53-53
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    • 2021
  • 본 연구에서는 수심센서가 장착된 고성능 GPS 전자부자를 활용하여 하천 수리량 측정에 대한 방법과, 개발 된 고성능 GPS 전자부자의 성능 검증을 위해서 수리량 측정장비인 Flowtracker 측정데이터와 비교분석을 하고자 하였다. 국내 하천환경에서의 수리량 조사는 필수적인 요소이다. 최근 국내 첨단기술을 활용한 수리량 계측 기술들은 ADCP, ADV, UVM, SIV와 같은 많은 계측 기술들이 있다. 이러한 기술들은 장비가 매우고가이며, 많은 시간과 인력이 필요하고 상황에 따른 한계점들이 존재한다. 이러한 수리량 계측 기술들은 오일러리안 타입 계측 방식의 측정으로 계측 구간이 한정되어 있어 수질 오염사고와 같은 장구간의 지속적인 정보가 필요한 하천상황에 따라 라그랑지안 방식의 계측도 필요하다. 본 연구에서 개발 된 고성능 GPS 전자부자는 IoT 기술이 접목되어 실시간 데이터 활용이 가능하며 실시간 데이터 분석을 통해 라그랑지안 타입의 장구간 계측이 가능하다. 고성능 GPS 전자부자는 외경 197mm 지름 88mm, 무게 700g 이내로 간편하게 소지 및 사용 시 투척이 간단하여 인력 및 현장 접근성에 대한 장점을 가지고 있다. 또한 기존 선행 연구들의 GPS 전자부자들의 경우 홍수 발생 시에 유량 측정에서 어려움이 있었기 때문에 본 연구에서 개발된 고서은 GPS 전자부자의 경우 수심센서의 장착으로 유량까지 산정할 수 있기 때문에 홍수 발생이나, 오염물질 발생으로 인한 접근성이 어려운 하천상황에서 유용하게 사용될 수 있다. 고성능 GPS 전자부자를 활용하여 중·소하천인 경북 김천 및 구미시에 위치한 감천에서 성능 평가를 진행하였으며 Flowtracker 수리량 측정 데이터 대비 오차율이 10% 이내로 들어오는 것을 확인하였다. 이러한 성능 검증을 바탕으로 고성능 GPS 전자부자를 활용한 측정법을 제시하였다.

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A four year study of the personal and interpersonal value systes of freshman nursing students at Yonsei University (서울 시내 일 간호대학 입학생의 4년간 개인 및 대인가치관의 횡단적 조사 연구)

  • Choe, Yeon-Sun
    • The Korean Nurse
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    • v.30 no.5
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    • pp.61-74
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    • 1991
  • The purpose of this study was to determine the values of freshman nursing students and to determine changes each year over the four years of the study and to provide base data for curriculum and student guidance. The sample consisted of the freshman students who entered college of nursing from 1988 through 1991. The total sample was 358 students and data were collected during March of each of the four years. The data were analyzed using t-test, ANOVA and Pearson Correlation to examine the relationship between the subcategories of values and Duncan multiple range test to examine the relationship between the scores for each year and the demographic data. The instrument used in this study was the Survey of Personal Values and Interpersonal Values modified by UngYun Hwang and KyungHae Lee in accordance with L.V. Gordon's instrument. This tool provides scores on practical mindedness, achievement, variety, decisiveness, orderliness, goal orientation, support, conformity, recognition, independence, benevolence and leadership. The results of the study were as follows: 1. The nursing freshman students surveyed had the highest score for goal orientation (21.44) and the lowest for variety (17.13) on the SPY (Survey of Personal Values) and the highest score for benevolence (21.19) and lowest for leadership (17.00) on the SIV (Survey of Interpersonal Values]. In accordance with the standarized scores for Gordon's Spy for other' woman college students, the students in the study were between the 78% ile - 97 % ile and were in the 72 % ile 97%ile for the SlV. 2. When t-test scores were compared, no significant variation was found between the years for each of the values (P< .05). 3. A significant variation was found on variety, between 1989 and 1991 (P< .05), and between 1988 and 1991. The highest score was in 1991 in both comparisons. Supporting was also higher for 1991 than for 1988 (P< .05). 4. Religion and occupation of father did not show any significant differences (P< .05). There was no significant difference in values according to the student's religion or the occupation of their father. Students born in the metropolitan area had a higher score on variety than those from the rural area and vice versa on regulartiy (P< .05). The results of this study show that there is a higher score on values for'! University freshman than shown for freshmen in other universities reported from other research, To keep or promote values, it is very important that there be a systematic strategy of education and special educational programs.

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One-Stage Polymerase Chain Reaction for the Comprehensive Detection of Type D Retrovirus Provial DNA (Type D Retrovirus 감염의 포괄적 검색을 위한 One-Stage 중합효소 연쇄반응법의 개발)

  • Jeong, Yong-Seok
    • The Journal of Korean Society of Virology
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    • v.27 no.1
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    • pp.19-27
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    • 1997
  • To develop the polymerase chain reaction (PCR) for the detection of type D simian retrovirus (SRV) infection, an oligonucleotide primer pair was designed to hybridize to the sequences within env gene of SRV subtype 1 (SRV-1). The 3' proximal env sequences annealing to the primers had been rather conserved among three different subtypes of SRV, SRV-1, SRV-2, and SRV-3 (Mason-Pfizer Monkey Virus: MPMV). The PCR using the primer pair targeting an env region successfully detected and amplified all three subtypes of SRV with excellent specificity after single round of reaction. The tests with peripheral blood mononuclear cells infected either with simian immunodeficiency virus or simian T-Iymphotropic virus type 1, major immunosuppressive viral agents together with SRV in simian, verified the specificity of the PCR by excluding any cross reactivity. Semiquantitative titration PCR, amplifying serially diluted plasmid DNA of each subtype, was performed to evaluate sensitivity limits of the reaction. Based on molecular weight of each cloned SRV genome, the PCR should be able to detect one SRV-infected cell per more than $5-7{\times}10^4$ uninfected cells after simple ethidium bromide staining of resulting products. The PCR must be very efficient screening system with its quickness, certainty, and sensitivity for SRV-infected animals used in human AIDS research model. Second round amplification of the reaction products from the first PCR, or Southern hybridization by radiolabeled probes shall render to compete its efficacy to ELISA which has been the most sensitive technique to screen SRV infection but with frequent ambiguity problem.

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