• Mass Spectrometry Letters
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• v.11 no.4
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• pp.108-112
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• 2020

A certified reference material (CRM) for the analysis of nutrient elements in an edible mushroom (Ganoderma lyceum) powder has been developed (KRISS CRM 108-10-011). The mass fractions of calcium (Ca), iron (Fe), and zinc (Zn) were measured by isotope dilution inductively coupled plasma mass spectrometry (ID ICP/MS). To dissolve the fungi cell wall of mushroom consisted of chitin fibers, sample preparation method by single reaction chamber type microwave-assisted acid digestion with acid mixtures was optimized. The mean measurement results obtained from 12 sample bottles were used to assign as the certified values for the CRM and the between-bottle homogeneities were evaluated from the relative standard deviations. The certified values were metrologically traceable to the definition of the kilogram in the International System of Units (SI). This CRM is expected to be used for validation of analytical methods or quality control of measurement results in analytical laboratories when they determine the mass fractions of elements in mushroom or other similar samples.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1077-1086
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• 2001

The sprA and sprB gene encoding chymotrypsin-like proteases Streptomyces griseus protease A (SGPA) and Streptomyces griseus protease B (SGPB) and the sprT gene that encodes Streptomyces griseus trypsin (SGT) were cloned from Streptomyces griseus ATCC10137 and overexpressed in Streptomyces lividans TK24 as a heterologous host. The chymotrypsin activity of tole culture broth measured with the artificial chromogenic substrate , N-succinyl-ala-ala-pro-phe-p-nitroanilide, was 10, 14 and 14 units/mg in the transformants haboring the sprA, sprB and sprD genes, respectively. The growth of S. lividans reached the maximum cell mass after 4 days of culture, yet SGPA and SGPD production started in the stationary phase of cell growth and kept increasing for up to 10 days of culture in an R2YE medium. The trypsin activity of the culture broth measured with the artificial chromogenic substrate , N-${\alpha}$-benzoyl-DL- arginine-p-nitroanilide , was 16 units/mg and SGT production started in the stationary phase of cell growth and kept increasing for up to 10 days of culture in an R2YE medium. The introduction of the sprA gene into S, lividans TK24 triggered the biosynthesis of pigmented antibiotics, actinorhodin and undecylprodigiosin, and induced significant morphological changes in the colonies in Benedict, R2YE, and R1R2 media. In addition, the introduction of the sprT gene also induced morphological changes in the colony shape without affecting the antibiotic production, thereby implying that certain proteases would appear to play very important and specific roles in secondary-metabolites formation and morphological differentiation in Streptomyces.

• BMB Reports
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• v.32 no.1
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• pp.86-91
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• 1999

The sprT gene encoding Streptomyces griseus trypsin (SGT) was introduced into Streptomyces lividans TK24 and Streptomyces lividans 1326 to study which strain would be better to overexpress the extracellular proteinase. Various media with different compositions were also used to maximize the productivity of SGT in heterologous hosts. The SGT productivity was best when the transformants of S. lividans TK24 and 1326 were cultivated in R2YE medium, and their relative trypsin activity of the culture broth measured with an artificial chromogenic substrate, N-${\alpha}$-benzoyl-DL-arginine-${\rho}$-nitroanilide, were 382 units/ml and 221 units/ml, respectively. They produced high levels of SGT in GYE medium but relatively lower than those in R2YE medium, and negligible amount of SGT was produced in Ferm, RASF, LIVID, and NDSK media. Considering non-SGT associated activity in Pronase powder, it was estimated that the transformant of S. lividans TK24 can produce SGT in R2YE 3.5 times more than the amount by S. griseus 10137 from which the sprT gene had been originated. The growth of S. lividans reached the maximum level of cell mass at 5 d of culture, but SGT production started in the stationary phase of cell growth and kept increasing until the ninth day of culture in R2YE medium, but in GYE media the productivity reached at the maximum level at 7 d of cultivation.

• Korean Journal of Food Science and Technology
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• v.11 no.4
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• pp.249-257
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• 1979

Using the whole cell immobilized glucose isomerase which was prepared in the previous work (Korean J. Food Sci. & Technol., 11(3), 192 (1979), the specific activity of the immobilized enzyme was 48.1 units in the batch reaction system and 114 units in the continuous reaction system per g of matrix, respectively. In the continuous reactor the voidity was 0.36, which was suitable for the packed bed reactor. This immobilized enzyme showed a good operational stability of 115 days of half life which was sufficient for the continuous operation. The experimental result showed that 55 % of the substrate was converted to the product in the packed bed reactor. The productivity was dependent on the flow rate, column geometry, enzyme loading, and substrate concentration. An intrapaticle diffusion was observed by the effectiveness factor of 0.75 and interparticle diffusion by the decrease of Km' with increasing the superficial velocity.

• Proceedings of the KSR Conference
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• 2008.11b
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• pp.1933-1942
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• 2008

Over the years, the Social Republic of Vietnam has demonstrated its high annual growth rate over 8%. In order to secure its sustainable economic growth, its has also demonstrated its sharp concerns on building up core infrastructures by luring foreign investments on a large scale, which would underpin the nation's economic propulsion. Among others, transport infrastructures and networks centering on railway systems are obviously seen as the most urgent to be built to continue its growth. Seoul Metro recently took a significant step forward in Vietnam to launch railway business by executing its export project of 6 units of metro cars to Hanoi as on July 10, 2008. Those 6 cars are scheduled to be put on the tracks linking Yen Bian, Hanoi with Ha Long, for demonstrative running, around October, 2008, carrying tourists on that 163kms of the National Railway line serving the region. A successful running on the track will duly entail further export of additional 54 units of Seoul Metro cars to Vietnam. In this thesis, description will be noted on the reuse and economic value of the Seoul Metro's de-commissioned metro cars, reflecting on the current urban railway law at home regulating the service life of rolling stocks domestically. This thesis will also study the points at issue, and the recommendable future orientation of the Vietnam railway business project.

• Journal of the Korea Institute of Building Construction
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• v.23 no.6
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• pp.809-820
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• 2023

In contemporary society, there is a notable rise in single-person households, coupled with an increased demand for residential spaces, necessitating the exploration of suitable housing solutions. Modular architecture is gaining prominence as a method to deliver cost-effective and sustainable living spaces efficiently. This study introduces a method for optimizing the design and construction processes of modular building units through Building Information Modeling(BIM) technology. It involves an analysis of factors that enhance living quality and meet the housing needs of single-person households. Utilizing BIM, a resource-efficient and cost-efficient modular apartment unit has been developed. BIM facilitates the visualization and integration of architectural components, thereby reducing design errors and enhancing productivity. This research evaluates both the benefits and constraints of modular architecture. Modular constructions, when combined with BIM, emerge as a potent solution for housing challenges, enriching the quality and variety of residential options. Nevertheless, considerations such as technical prerequisites and financial implications are also discussed. The study showcases the potential and merits of utilizing BIM for designing apartment units tailored to single-person households, offering a viable approach to ameliorate their living conditions.

• Journal of the Korean Association of Geographic Information Studies
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• v.16 no.3
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• pp.40-53
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• 2013

Most of the existing domestic studies to identify the distribution of longevity population and influencing factors oriented confirmatory approach. Furthermore, most of the studies in this research topic simply have used their own definition of spatial unit of analysis or employed arbitrary spatial units of analysis according to data availability. These research approaches can not sufficiently reflect the spatial characteristic of longevity phenomenon and exposed to the Modifiable Aerial Unit Problem(MAUP). This research performed the Exploratory Spatial Data Analysis(ESDA) to identify the spatial autocorrelation of the distribution of longevity population and investigated whether the modifiable areal unit problem in the aspect of scale effect using spatial population data in Korea. We used Si_Gun_Gu and Eup_Myeon_Dong as two different spatial units of regional longevity indicators measured. Then, we applied Getis-Ord Gi* to investigate the existence of spatial hot spots and cold spots. The results from our analysis show that there exist statistically significant spatial autocorrelation and spatial hot spots and cold spots of regional longevity at both Si_Gun_Gu and Eup_Myeon_Dong levels. This result implies that the modifiable areal unit problem does exist in the studies of spatial patterns of longevity population distribution. The demand for longevity researches would be increased inevitably. In addition, there were apparent differences for the global spatial autocorrelation and local spatial cluster which calculated different spatial units such as Si_Gun_Gu and Eup_Myeon_Dong and this can be seen as scale effect of MAUP. The findings from our analysis show that any study in this topic can mislead results when the modifiable areal unit problem and spatial autocorrelation are not explicitly considered.

• Journal of Korean Society of Forest Science
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• v.113 no.1
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• pp.51-65
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• 2024

Phyto-sociological methods were used in this study to assess the vegetation structure of a forest stand at Mt. Goehwa in Sejong-Si with the aim of providing vegetation information for urban forest utilization and management plans. The actual forest vegetation was classified into two types of community groups (Quercus serrata-Lindera obtusiloba and Coreopsis lanceolata community groups) at the highest hierarchical level. The Q. serrata-L. obtusiloba community group was classified into six units, which included artificial forest and natural forest vegetation. Artificial forests were classified into three communities (Pinus rigida, Castanea crenata, and Robinia pseudoacacia), whereas natural forests were classified into three communities (Quercus variabilis, Quercus acutissimaa, and Pinus densiflora). The Coreopsis lanceolata community group, which exhibited vegetative characteristics of urban forest edge areas, was categorized into four units. The urban forest edges were classified into four communities (Indigofera bungeana, Lespedeza bicolor, Amorpha fruticosa, and Lespedeza cuneata). Accordingly, the vegetation structure of Mt. Goehwa was categorized into 10 vegetation unit systems. An importance value analysis showed the highest importance value for C. crenata at 6.7%, followed by P. rigida at 6.4%, and R. pseudoacacia at 6.3%, indicating that the ecological impact of plantation species can be significant on Mt. Goehwa. A community coefficient of similarity analysis revealed that the artificial and natural forests had similar species compositions; however, both forests differed from the urban forest edge. This variation was further confirmed by Detrended correspondence analysis(DCA), with similar results. Canonical correspondence analysis(CCA) showed that the artificial forest and natural forest community types were positively correlated with altitude, bare rock, and the present species. By contrast, the urban forest edge community types were negatively correlated with these factors.

• The Journal of the Korea Contents Association
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• v.9 no.3
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• pp.250-255
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• 2009

This study performed and evaluated the performance test in 40 general X-ray units among diagnostic X-ray units, which were being used in hospitals of gyeongsangnam-do gimhae-si through X-ray tube variable limiting device ability test, the light field and X-ray field alignment test and collimation and beam alignment test of diagnostic X-ray unit. The results are as followings: In a variable beam limiting device ability test, the result of maximum X-ray field test showed that 4(10%) of were incongruent while the result of minimum X-ray field test represented that 5(12.5%) of were incongruent. The result of the light field and X-ray field alignment test showed 23(57.5%) of were within 2% of maximum permissible level and the other 17(42.5%) units were misalignment. The result of beam alignment test represented that 11(27.5%) coincided and another 11(27.5%) within $0.5^{\circ}$ respectively, 10(25%) were $0.6^{\circ}-1.5^{\circ}$ intervals, 7(17.5%) were $1.6^{\circ}-3^{\circ}$ and 1(2.5%) were more than $3^{\circ}$.

• Journal of Environmental Impact Assessment
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• v.28 no.6
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• pp.580-591
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• 2019

This study analyzed roadkill hotspots in Yeongju, Mungyeong-si Andong-si and Cheongsong-gun to compare the method of searching the area of the spatial cluster for selecting the roadkill hotspots. The local spatial autocorrelation index Getis-Ord Gi* statistics were calculated by different units of analysis, drawing hotspot areas of 9% from 300 m and 14% from 1 km on the basis of the total road area. The rating of Z-score in the 1km hotspot area showed the highest Z-score in the 28th National Road section on the border between Yecheon-gun and Yeongj-si. The kernel density method performed general kernel density estimation and network kernel density estimation analysis, both of which made it easier to visualize roadkill hotspots than district unit analysis, but there were limitations that it was difficult to determine statistically significant priority. As a result, local hotspot areas were found to be different according to the cluster analysis method, and areas that are in common need of reduction measures were found to be the hotspot of 28th National Road through Yeongju-si and Yecheon-gun. It is deemed that the results of this study can be used as basic data when identifying roadkill hotspots and establishing measures to reduce roadkill.