• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3811-3816
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• 2015

Background: Hairy and enhancer of split 1 (Hes-1) protein is a downstream target of Notch signaling and is a basic helix-loop-helix transcriptional repressor. However, definitive evidence for a role in hepatocellular carcinoma (HCC) cells has not been reported. Here, Hes-1 was revealed to an important component of the Notch signaling cascade in HCC cell lines possessing different potential for lung metastasis. Materials and Methods: RNAi mediated by plasmid constructs was used to analyze the role of Hes-1 in MHCC-97L HCC cells by assessing proliferation, apoptosis, cell migration and matrigel invasion following transfection. Hes-1 protein expression analysis in HCC tissue was also conducted by immunohistochemistry. Results: Our studies revealed that Hes-1 was decreased in HCC cell lines with higher lung metastasis potential at both the mRNA and protein levels. Down-regulation of the Hes-1 gene in MHCC-97L cells resulted in increased cell proliferation, reduced apoptosis and increased migration and invasion. Conclusions: Hes-1 has potential prognostic value in post-surgical HCC patients and may be an independent prognostic indicator for overall survival and tumor recurrence. These findings have important implications for understanding the mechanisms by which Hes-1 participates in tumor proliferation and invasion.

• Applied Biological Chemistry
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• 제39권2호
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• pp.140-146
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• 1996

Imidacloprid와 일련의 새로운 1-(6-chloro-3-pyridylmethy)-3-N-nitroimidazolidin-2-ylideneamine 유도체들을 합성하고 imidazolidine고리의 3-N-치환기(R) 변화에 따른 구조와 벼멸구(Nilaparvata lugens) 및 복숭아 혹 진딧물(Myzus persicae)에 대한 선택적인 살충활성 관계(SAR)를 검토한 바, 벼멸구에는 STERIMOL 파라미터인 B1상수$({\AA})$보다 소수성$({\pi})$, 그리고 복숭아 혹 진딧물에는 입체상수(Es)보다 극성 치환기상수$({\sigma}^{ast})$ 큰 변수로 작용하였다. 벼멸구의 경우에는 ${\pi}$상수의 적정값이 0.52이고 복숭아 혹 진딧물의 경우에는 $\sigma^{\ast}$상수의 적정값이 1.17로 이들 값에 가까운 값을 갖는 치환체일수록 큰 살충활성이 예상되었다. 두 경우, 모두 공통적으로 methyl 및 benzenesulfonyl-치환체(7 및 8)와 같은 센 전자끌게들은 살충활성에 기여하지 않았다. 그러나, 비치환(H)체(1)인 imidacloprid는 가장 큰 살충활성을 나타내었으며 H의 ${\pi}$상수와 ${\sigma}^{\ast}$상수가 이들 두 상수의 적정값에 미치지 못하지만, 양호한 살충활성을 나타낸 까닭은 2-N-nitro group의 산소원자와 3-imid group의 수소원자 사이에 형성된 분자내 회합(H-결합)에 기인함을 알았다. 그리고 pH 6.0 이하의 산성 수용액에서는 대단히 큰 잔류성을 나타내었으며 $45^{\circ}C$의 pH 7.0에서$(K_{obs};5{\times}10^8sec.^{-1})$ 반감기(t1/2)는 대략 6개월이었다.

• 한국발생생물학회지:발생과생식
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• 제13권3호
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• pp.173-181
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• 2009

One of the most extensively studied populations of multipotent adult stem cells are mesenchymal stem cells (MSCs). MSCs derived from the human umbilical cord vein (HUC-MSCs) are morphologically and immunophenotypically similar to MSCs isolated from bone marrow. HUC-MSCs are multipotent stem cells, differ from hematopoietic stem cells and can be differentiated into neural cells. Since neural tissue has limited intrinsic capacity of repair after injury, the identification of alternate sources of neural stem cells has broad clinical potential. We isolated mesenchymal-like stem cells from the human umbilical cord vein, and studied transdifferentiation-promoting conditions in neural cells. Dopaminergic neuronal differentiation of HUC-MSCs was also studied. Neural differentiation was induced by adding bFGF, EGF, dimethyl sulfoxide (DMSO) and butylated hydroxyanisole (BHA) in N2 medium and N2 supplement. The immunoreactive cells for $\beta$-tubulin III, a neuron-specific marker, GFAP, an astrocyte marker, or Gal-C, an oligodendrocyte marker, were found. HUC-MSCs treated with bFGF, SHH and FGF8 were differentiated into dopaminergic neurons that were immunopositive for tyrosine hydroxylase (TH) antibody. HUC-MSCs treated with DMSO and BHA rapidly showed the morphology of multipolar neurons. Both immunocytochemistry and RT-PCR analysis indicated that the expression of a number of neural markers including NeuroD1, $\beta$-tubulin III, GFAP and nestin was markedly elevated during this acute differentiation. While the stem cell markers such as SCF, C-kit, and Stat-3 were not expressed after neural differentiation, we confirmed the differentiation of dopaminergic neurons by TH/$\beta$-tubulin III positive cells. In conclusion, HUC-MSCs can be differentiated into dopaminergic neurons and these findings suggest that HUC-MSCs are alternative cell source of therapeutic treatment for neurodegenerative diseases.