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Characterization of Prophages in Leuconostoc Derived from Kimchi and Genomic Analysis of the Induced Prophage in Leuconostoc lactis

  • Kim, Song-Hee;Park, Jong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.32 no.3
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    • pp.333-340
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    • 2022
  • Leuconostoc has been used as a principal starter in natural kimchi fermentation, but limited research has been conducted on its phages. In this study, prophage distribution and characterization in kimchi-derived Leuconostoc strains were investigated, and phage induction was performed. Except for one strain, 16 Leuconostoc strains had at least one prophage region with questionable and incomplete regions, which comprised 0.5-6.0% of the bacterial genome. Based on major capsid protein analysis, ten intact prophages and an induced incomplete prophage of Leu. lactis CBA3626 belonged to the Siphoviridae family and were similar to Lc-Nu-like, sha1-like, phiMH1-like, and TPA_asm groups. Bacterial immunology genes, such as superinfection exclusion proteins and methylase, were found on several prophages. One prophage of Leu. lactis CBA3626 was induced using mitomycin C and was confirmed as belonging to the Siphoviridae family. Homology of the induced prophage with 21 reported prophages was not high (< 4%), and 47% identity was confirmed only with TPA_asm from Siphoviridae sp. isolate ct3pk4. Therefore, it is suggested that Leuconostoc from kimchi had diverse prophages with less than 6% genome proportion and some immunological genes. Interestingly, the induced prophage was very different from the reported prophages of other Leuconostoc species.

Joint Relay Selection and Resource Allocation for Delay-Sensitive Traffic in Multi-Hop Relay Networks

  • Sha, Yan;Hu, Jufeng;Hao, Shuang;Wang, Dan
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.16 no.9
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    • pp.3008-3028
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    • 2022
  • In this paper, we investigate traffic scheduling for a delay-sensitive multi-hop relay network, and aim to minimize the priority-based end-to-end delay of different data packet via joint relay selection, subcarrier assignment, and power allocation. We first derive the priority-based end-to-end delay based on queueing theory, and then propose a two-step method to decompose the original optimization problem into two sub-problems. For the joint subcarrier assignment and power control problem, we utilize an efficient particle swarm optimization method to solve it. For the relay selection problem, we prove its convexity and use the standard Lagrange method to deal with it. The joint relay selection, subcarriers assignment and transmission power allocation problem for each hop can also be solved by an exhaustive search over a finite set defined by the relay sensor set and available subcarrier set. Simulation results show that both the proposed routing scheme and the resource allocation scheme can reduce the average end-to-end delay.

Implementation and benchmarking of the local weight window generation function for OpenMC

  • Hu, Yuan;Yan, Sha;Qiu, Yuefeng
    • Nuclear Engineering and Technology
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    • v.54 no.10
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    • pp.3803-3810
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    • 2022
  • OpenMC is a community-driven open-source Monte Carlo neutron and photon transport simulation code. The Weight Window Mesh (WWM) function and an automatic Global Variance Reduction (GVR) method was recently developed and implemented in a developmental branch of OpenMC. This WWM function and GVR method broaden OpenMC's usage in general purposes deep penetration shielding calculations. However, the Local Variance Reduction (LVR) method, which suits the source-detector problem, is still missing in OpenMC. In this work, the Weight Window Generator (WWG) function has been developed and benchmarked for the same branch. This WWG function allows OpenMC to generate the WWM for the source-detector problem on its own. Single-material cases with varying shielding and sources were used to benchmark the WWG function and investigate how to set up the particle histories utilized in WWG-run and WWM-run. Results show that there is a maximum improvement of WWM generated by WWG. Based on the above results, instructions on determining the particle histories utilized in WWG-run and WWM-run for optimal computation efficiency are given and tested with a few multi-material cases. These benchmarks demonstrate the ability of the OpenMC WWG function and the above instructions for the source-detector problem. This developmental branch will be released and merged into the main distribution in the future.

Design of the flexible switching controller for small PWR core power control with the multi-model

  • Zeng, Wenjie;Jiang, Qingfeng;Du, Shangmian;Hui, Tianyu;Liu, Yinuo;Li, Sha
    • Nuclear Engineering and Technology
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    • v.53 no.3
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    • pp.851-859
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    • 2021
  • Small PWR can be used for power generation and heating. Considering that small PWR has the characteristics of flexible operating conditions and complex operating environment, the controller designed based on single power level is difficult to achieve the ideal control of small PWR in the whole range of core power range. To solve this problem, a flexible switching controller based on fuzzy controller and LQG/LTR controller is designed. Firstly, a core fuzzy multi-model suitable for full power range is established. Then, T-S fuzzy rules are designed to realize the flexible switching between fuzzy controller and LQG/LTR controller. Finally, based on the core power feedback principle, the core flexible switching control system of small PWR is established and simulated. The results show that the flexible switching controller can effectively control the core power of small PWR and the control effect has the advantages of both fuzzy controller and LQG/LTR controller.

Accessibility of Korean Cultural Values at Screens: Decoding Patterns of North American Audiences

  • Xiuli, Chen;Sirui, Yao;Jiyeon, Baek;Jiena, Sha;Kesu, Lin;Kyung-young, Chung
    • International Journal of Advanced Culture Technology
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    • v.10 no.4
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    • pp.153-159
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    • 2022
  • This research investigates the accessibility of Korean modern values at screens from the perspectives of North American audiences while consuming Korean films and TV dramas. In order to decode Korean cultural values at screens, this study collects the data of 266 Korean films and TV dramas distributed in North America from IMDb platform, unlocking that the success of box office for Korean projects in North America is positively correlated with the characteristics of Violence & Gore and Profanity. Meanwhile, the audiences' positive responses to the violent project mainly depends on the degree of "Sex and Nudity", the plots of "social groups". The current success of Korean cultural and creative industry as well as the future global markets strengths are analyzed in this research. Stuart Hall's cultural studies approach and cultural psychological account theory are applied to discuss the success of Korean entertainment industry in the transnational cultural communication.

Dynamic characteristics monitoring of a 421-m-tall skyscraper during Typhoon Muifa using smartphone

  • Kang Zhou;Sha Bao;Lun-Hai Zhi;Feng Hu;Kang Xu;Zhen-Ru Shu
    • Structural Engineering and Mechanics
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    • v.87 no.5
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    • pp.451-460
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    • 2023
  • Recently, the use of smartphones for structural health monitoring in civil engineering has drawn increasing attention due to their rapid development and popularization. In this study, the structural responses and dynamic characteristics of a 421-m-tall skyscraper during the landfall of Typhoon Muifa are monitored using an iPhone 13. The measured building acceleration responses are first corrected by the resampling technique since the sampling rate of smartphone-based measurement is unstable. Then, based on the corrected building acceleration, the wind-induced responses (i.e., along-wind and across-wind responses) are investigated and the serviceability performance of the skyscraper is assessed. Next, the amplitude-dependency and time-varying structural dynamic characteristics of the monitored supertall building during Typhoon Muifa are investigated by employing the random decrement technique and Bayesian spectral density approach. Moreover, the estimated results during Muifa are further compared with those of previous studies on the monitored building to discuss its long-term time-varying structural dynamic characteristics. The paper aims to demonstrate the applicability and effectiveness of smartphones for structural health monitoring of high-rise buildings.

Potential Mechanisms of Benzyl Isothiocyanate Suppression of Invasion and Angiogenesis by the U87MG Human Glioma Cell Line

  • Zhu, Yu;Zhang, Ling;Zhang, Guo-Dong;Wang, Hong-Ou;Liu, Ming-Yan;Jiang, Yuan;Qi, Li-Sha;Li, Qi;Yang, Ping
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.19
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    • pp.8225-8228
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    • 2014
  • Glioma is one of the most common tumors in China and chemotherapy is critical for its treatment. Recent studies showed that benzyl isothiocyanate (BITC) could inhibit the growth of glioma cells, but the mechanisms are not fully understood. This study explored the inhibitory effect of BITC on invasion and angiogenesis of U87MG human glioma cells in vitro and in vivo, as well as potential mechanisms. It was found that BITC could inhibit invasion and angiogenesis of human glioma U87MG cells by inducing cell cycle arrest at phase G2/M. It also was demonstrated that BITC decreased expression of cyclin B1, p21, MMP-2/9, VE-cadherin, CD44, CXCR4 and MTH1, the activity of the telomerase and $PKC{\zeta}$ pathway. Microarray analysis was thus useful to explore the potential target genes related to tumorigenic processes. BITC may play important roles in the inhibition of invasion and angiogenesis of human glioma cells.

Graphitization of PAN-based carbon fibers by CO2 laser irradiation

  • Yao, Liangbo;Yang, Weimin;Li, Sanyang;Sha, Yang;Tan, Jing;An, Ying;Li, Haoyi
    • Carbon letters
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    • v.24
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    • pp.97-102
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    • 2017
  • Graphite fibers are materials with a high specific modulus that have attracted much interest in the aerospace industry, but their high manufacturing cost and low yield are still problems that prevent their wide applications in practice. This paper presents a laser-based process for graphitization of carbon fiber (CF) and explores the effect of laser radiation on the microstructure of CF. The obtained Raman spectra indicate that the outer surface of CF evolves from turbostratic structures into a three-dimensional ordered state after being irradiated by a laser. The X-ray diffraction data revealed that the growth of crystallite was parallel to the fiber axis, and the interlayer spacing $d_{002}$ decreased from 0.353 to 0.345 nm. The results of scanning electron microscopy revealed that the surface of irradiated CFs was rougher than that of the unirradiated ones and there were scale-like small fragments that had peeled off from the fibers. The tensile modulus increased by 17.51% and the Weibull average tensile strength decreased by 30.53% after being irradiated by a laser. These results demonstrate that the laser irradiation was able to increase the graphitization degree of the CFs, which showed some properties comparable to graphite fibers.

Domestic and Foreign Research Trend on the Pediatric Chuna Treatment (소아 추나에 대한 국내·외 연구 동향)

  • Lee, Jin Hwa;Han, Jae Kyung;Kim, Yun Hee
    • The Journal of Pediatrics of Korean Medicine
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    • v.29 no.4
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    • pp.67-76
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    • 2015
  • Objectives The purpose of this review is to investigate the domestic and foreign studies of pediatric Chuna treatment and propose the directions of future studies and clinical applications. Methods We searched for the study at RISS, KISS, DBPIA, Pubmed, CNKI by keywords, '추나', 'Osteopathic', 'Chiropractic', 'Manipulation', '推拿', '導引', '按摩', After 2010. Results 1. Selected 3 domestic studies were categorized as 1 survey study and 2 case reports. Selected 41 foreign studies from Pubmed were categorized as 15 systemic reviews, 8 survey studies, 12 case reports and 6 control studies. Selected 82 foreign studies from CNKI were categorized as 10 systemic reviews, 22 case reports and 50 control studies. 2. 2 clinical domestic studies researched on idiopathic Scoliosis. The foreign clinical studies from Pubmed are 18 cases, and those studies were categorized into Premature baby care (3), Infant colic (2), ADHD (2), Congenital talipes equinovarus (1), Somatic dysfuntion (1), Nonsynostotic occipital plagiocephaly (1), Conversion disorder (1), Lower back pain (1), Chronic bilateral dorsal foot pain and stiffness (1), plantar fasciitis (1), Migraine headaches (1), Cyclic vomiting syndrome (1), Acute otitis media (1) and Cerebral palsy (1). The other 72 foreign clinical studies were from CNKI, and they studied 39 different diseases. Systematically, they studied about digestive diseases (25), respiratory diseases (20), fever (6), musculoskeletal diseases (5), nervous system diseases (5), dermatology diseases (2) and other disease states. The Chuna treatment was used in variety of studies. 3. 2 clinical domestic studies adopted techniques of Osteopathy Chuna. The foreign clinical studies from Pubmed adopted techniques of Osteopathic manipulation (10) and Chiropractic manipulation (8). The other foreign clinical studies from CNKI adopted techniques of Acupressure (69), Abdominal manipulation (23), Spinal manipulation (21), Thoracic manipulation (11), Traction manipulation (2), Muscular manipulation (2), Squeezing Sha manipulation (1), Spine correction (1), Joint manipulation (1) and Fascia manipulation (1). Conclusions In addition to musculoskeletal disorders, variety of pediatric diseases could be treated with Chuna treatment instead of acupuncture.

Somatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits

  • Shang, Jiang-Hua;Xu, Ru-Xiang;Jiang, Xiao-Dan;Zou, Yu-Xi;Qin, Ling-Sha;Cai, Ying-Qian;Yang, Zhi-Jun;Zheng, Xing;Cui, Sheng
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.9
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    • pp.1354-1360
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    • 2007
  • The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients for somatic cell nuclear transfer (SCNT). Within a common SCNT protocol, a comparison between oocytes recovered by direct aspiration (aspirated) from available ovarian follicles and oocytes flushed out from oviducts (flushed) was carried out. The results showed that maturation and enucleation rates of aspirated oocytes were 70.7% and 69.2%, significantly lower than 95.3% (p<0.01) and 83.6% (p<0.05), respectively, from flushed oocytes. However, following enucleation of matured oocytes as ooplasm recipients for SCNT, no difference was recorded in fusion and cleavage rates, as well as blastocyst development from cleaved embryos or hatching of blastocysts between aspirated and flushed groups. Additionally, some matured aspirated and flushed oocytes were also used for immediate parthenogenetic activation and the resulting embryo development was not significantly different. Results from this study show the following: i) the majority of oocytes aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration are matured and can be used directly as ooplasm recipients for SCNT; ii) the reconstructed embryos derived from these oocytes have similar in vitro developmental ability to those flushed from the oviducts.