• Journal of Microbiology and Biotechnology
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• 제23권4호
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• pp.579-585
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• 2013

Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is an important pathogen associated with opportunistic infections of a wide range of species, including pigs and humans. The absence of a suitable vaccine makes it difficult to control SEZ infection. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been previously identified as an immunogenic protein using immunoproteomic techniques. In the present study, we confirmed that the sequence of GAPDH was highly conserved with other Streptococcus spp. The purified recombinant GAPDH could elicit a significant humoral antibody response in mice and confer significant protection against challenge with a lethal dose of SEZ. GAPDH could adhere to the Hep-2 cells, confirmed by flow cytometry, and inhibit adherence of SEZ to Hep-2 cells in an adherence inhibition assay. In addition, real-time PCR demonstrated that GAPDH was induced in vivo following infection of mice with SEZ. These suggest that GAPDH could play an important role in the pathogenesis of SEZ infection and could be a target for vaccination against SEZ.

• 한국경제지리학회지
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• 제19권1호
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• pp.122-142
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• 2016

이 연구는 1990년대 초반부터 시작된 북한의 경제특구 실험의 의의를 북한의 체제변화와 한반도 지정학적 질서의 변화 측면에서 살펴보았다. 구체적으로 북한 경제특구 정책의 변화과정을 북한 경제특구 전략 고유의 영역화 논리, 분권화/분산화에 대한 강조, 그리고 중국 개혁개방모델과의 비교 등 세 가지 측면을 중심으로 분석하였다. 이를 통해 북한 경제체제의 변화를 폐쇄에서 개방으로 이해하는 단선적 접근을 비판하고, 북한 특구전략의 역동성을 다층적인 차원에서 접근하였다. 북한의 경제특구전략을 경제난에 의한 불가피한 선택이나 중국과 같은 개혁개방 노선을 따르는 것으로 이해할 수는 없으며, 오히려 특구전략 기저에 작동하고 있는 지정학적 논리와 함께 지방경제발전에 대한 강조를 중심으로 보아야 한다고 주장하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권7호
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• pp.1104-1110
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• 1999

We condicted two experiments to evaluate the interaction among fumaric acid (FA), protected acids (PA), or no additional acid (NO) and two different levels of acid buffering capacity (BC) in diets for 14-d-old weaned pigs. BC was varied substituting mono-calcium phosphate and calcium sulfate for dicalcium phosphate and calcium carbonate. In the high BC diet plus PA, FA was also added. In Exp. 1, 48 gilts were raised for 31 days on the six diets, evaluating growth performance and fecal digestibility. In Exp. 2, 42 gilts were raised. With each diet three subjects were sacrificed after 19 days and four after 38 days. In addition, six subjects were sacrificed at weaning. Growth and carcass performance, ileal digestibility, bacterial populations and pH in the gut were assessed. The piglet performance and stomach, ileal and cecal pH, and empty body composition were not affected by the diets. Empty body composition other than ash content was affected by piglet age (p<0.01). The BC did not influence digestibility. The dietary inclusion of PA improved fecal digestibility of protein (p<0.05) compared to the addition of FA and NO. Ileal digestibility slightly increased with both acid additions (p<0.10), the groups receiving PA showing the higher values. Piglets fed diets with low BC had lower Lactobacillus and E. coli counts in the ileum (p=0.07) and higher Lactobacillus in the colon (p=0.08). Acidified diets tended to reduce E. coli counts in the ileum (p=0.10) and increased Lactobacillus in the colon (p=0.09). The addition in the diet of PA increased Lactobacillus in the ileum compared to the sole addition of free fumaric acid (p=0.07). The addition of protected acids, combined with free fumaric acid in the case of high BC diets, increased protein digestibility and Lactobacillus counts and reduced E.coli counts. Only some changes in the concentration of bacterial population can be expected with a diet of low BC.

• Journal of Microbiology and Biotechnology
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• 제24권3호
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• pp.337-345
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• 2014

Pichia pastoris is one of the most widely used expression systems for the secretory expression of recombinant proteins. The secretory expression in P. pastoris usually makes use of the prepro $MAT{\alpha}$ sequence from Saccharomyces cerevisiae, which has a dibasic amino acid cleavage site at the end of the signal sequence. This is efficiently processed by Kex2 protease, resulting in the secretion of high levels of proteins to the medium. However, the proteins that are having the internal accessible dibasic amino acids such as KR and RR in the coding region cannot be expressed using this signal sequence, as the protein will be fragmented. We have identified a new signal sequence of 18 amino acids from a P. pastoris protein that can secrete proteins to the medium efficiently. The PMT1-gene-inactivated P. pastoris strain secretes a ~30 kDa protein into the extracellular medium. We have identified this protein by determining its N-terminal amino acid sequence. The protein secreted has four DDDK concatameric internal repeats. This protein was not secreted in the wild-type P. pastoris under normal culture conditions. We show that the 18-amino-acid signal peptide at the N-terminal of this protein is useful for secretion of heterologous proteins in Pichia.

• Asian-Australasian Journal of Animal Sciences
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• 제13권7호
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• pp.995-1002
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• 2000

To evaluate energy efficiency and partition of nutrients, 32 piglets were weaned at 14 d of age and individually fed diets containing 15% fat from coconut oil (CO, medium chain saturated), high oleate sunflower oil (HOSO, n-9 series), soybean oil (SO, n-6 series), or linseed oil plus fish oil, (LF, n-3 series). After 4 weeks, the subjects were sacrificed to evaluate empty body composition and apparent ileal digestibility with the slaughter method. No statistical effect of dietary fat sources on growth was observed. The digestibility of fat from the coconut oil diet was higher than fats from the diets containing high levels of unsaturated fatty acids. The efficiency of use of metabolizable energy for growth averaged 63% and was not affected by the diet. Dietary fat composition was reflected strongly in backfat. Total body neutral and polar fatty acids were influenced too. For the whole body phospholipid fraction the ratio of n-6 to n-3 and the double bond index were 4.3, 5.8, 7.2, 0.78 and 69, 87, 89, 87 for CO, HOSO, SO, and LF respectively. These results show that for the coconut oil diet the degree of unsaturation of phospholipids in the body was lower and that, in the other diets, it did not differ, but double bond index was maintained with different n-6 to n-3 ratios in carcass fat. On the whole the data on body fat composition indicate that the dietary fat tended to be deposited in similar quantity in the body, whatever was the dietary fatty acid profile.

• 대한수의학회지
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• 제47권4호
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• pp.409-415
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• 2007

An eight years old female barbary sheep (Ammotragus lervia), which bred at the Gwangju Uchi Park Zoo had shown anorexia, depression, respiratory problem for several weeks after parturition. In necropsy, extensive necrotizing pneumonia was found with severe immunocytes infiltration in the alveolar spaces and bronchioles. Pulmonary pleura were thickened with fibrin and inflammatory cells. Bacteria were isolated from lung and identified as Streptococcus equi subsp. zooepidemicus (SEZ) by biochemical tests and PCR on sodA and gusA genes, though seel gene was not detected. Isolation of zoonotic SEZ in public place such as a zoo should be emphasized for the public health mangagement.

• 토지주택연구
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• 제6권2호
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• pp.79-88
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• 2015

2010년 5월 북한의 김정일 위원장은 중국 베이징방문을 통하여 북 중 경협 강화와 경제특구 개발 가속화를 위해 라선과 황금평 위화도를 개발하기로 후진타오 주석과 공동인식을 달성하게 된다. 이것이 바로 "조중 공동개발 공동관리 라선경제무역지대와 황금평 경제지대"이다. 그러나 북 중 접경지역 경제협력의 대표적인 지역으로 발전과 변화를 기대했으나 2013년 2월 북한의 3차 핵실험과 더불어 시진핑(習近平) 국가 주석을 비롯한 중국 5세대 지도부의 북한에 대한 태도는 과거와 다른 냉랭함이 묻어 나오고 있다. 이는 북 중 관계의 흐름이 과거와 같지 않다는 것을 의미하는데, 황금평 경제지대 개발이 문서에만 그치고 있다는 점에서도 그러한 흐름을 발견할 수 있다. 라선경제무역지대의 경우 조중 공동관리위원회를 구성하여 일정한 수준의 발전과 변화를 보이고 있고, 최근에는 훈춘과 하산지역을 포함한 3국 국경 없는 국제관광구 등의 추진을 중국과 러시아에서 발표하고 있다. 물론 라선지대 개발계획에 비추어 그 변화는 상대적이지만 천천히 변화를 감지할 수 있는 모습을 보여주고 있다는 점에서 남 북 중 3자 협력의 가능성을 제기할 수 있다. 따라서 라선지역을 북한의 라선이 아니라 라선특별시 즉, 개방도시로 접근한다면 '5.24 조치'를 우회할 수 있는 길을 찾게 될 것이다. 이는 한 중 간 남 북 중 협력을 논할 수 있을 뿐만 아니라 북 중 간에도 우리가 남 북 중 협력을 논의하고 참여할 수 있는 방안을 마련해야 할 것이다. 대한민국의 새로운 블루오션을 구상하고 개척하기 위해 개방형 복합국가발전전략을 현실화시키는 전략적 선택이 필요한 시점이다.

• Journal of Contemporary Eastern Asia
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• 제20권2호
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• pp.278-297
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• 2021

Despite competing strategical interests over Southeast Asia that have emerged in the last decade, with the launch of wide scope geopolitical strategies Chinese and Japanese initiatives have been characterized by a certain degree of implicit coordination, particularly in offering support to the Myanmar state's territorializing strategies for economic development. The case of the Thilawa Special Economic Zones (SEZ) is exemplary, as it was a Japan-led project which became a model and benchmark example for similar development initiatives supported by the People's Republic of China.

• KDI Journal of Economic Policy
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• 제37권sup호
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• pp.87-121
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• 2015

한국정부는 FDI 국내 유치를 위해 외국인투자에 특례와 지원을 제공하는 경제특구를 도입하였으나 성과가 미흡하다는 문제의식이 존재한다. 따라서 본 논문에서는 실증분석을 통해 노동생산성, 영업이익률, 투자성향, 혁신성향, 고용성향 등의 기업단위 성과가 경제특구에 입주한 외국인투자기업과 국내기업 간에, 그리고 외국인투자기업들 간에 유의한 차이를 나타내고 있는지를 회귀분석과 PSM 분석을 통해 살펴보았다. 본 논문의 주요 결과는 다음과 같다. 첫째, 노동생산성 및 영업이익률 측면에서 살펴보았을 때 외국인투자기업들이 국내기업들에 비해 효율성 및 수익성에 있어 더 높은 성과를 나타낸다는 가설을 지지할 실증적 증거는 발견할 수 없었다. 둘째, 투자성향에 있어서는 외국인투자지역에 분포한 외국인투자기업들이 국내기업들에 비해 높은 투자성향을 보였다. 셋째, 연구개발투자 측면에 있어 외국인투자기업 전체적으로는 국내기업에 비해 높은 투자성향을 보인 것으로 나타나나 이것이 경제특구정책에 의한 것임을 나타내는 실증적 증거는 존재하지 않는다. 교육훈련투자에 있어서는 비특구 외국인투자기업의 역할이 가장 크며, 특구지역 중에서는 경제자유구역에 위치한 기업들의 교육훈련투자가 국내기업들보다 더 높다는 실증적 증거가 발견되었다. 마지막으로 고용성향에 있어서는 외국인투자기업들의 성과가 더 높았으나 이것이 경제특구정책에 의한 것임을 나타내는 실증적 증거가 존재하지는 않았다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권2호
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• pp.271-281
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• 1999

The cost of production of high quality pork is compensated by high returns, but constraints by the market are increasing. A few opportunities to maintain and improve pork quality by dietary means are presented. The healthy value of pork is a prerequisite. A careful control of suppliers and preservation of feeds are essential to protect pork against presence of contaminants from the feed. The feeding level and some dietary components modify the partition of the dietary energy into different pig tissues and chemical components of pork, affecting the hygienic and nutritional value, tenderness and taste. It is difficult to transfer a nutrient from the diet to pork, if the requirements. for growth are satisfied. Fatty acids and Vitamin E are the most studied exceptions. There is some evidence that iron and selenium contents can be affected too. Varying the content of a nutrient frequently changes sensorial and technological properties of pork. The addition of oils improves the acidic profile of depot fats, but the effect on phospholipid composition is not well studied and negative effects on oxidability and consistency of meat products are observed. Vitamin E can improve many healthy and sensorial characteristics, but its effect is clearer when the meat is stressed or manipulated.