• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.579-585
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• 2013

Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is an important pathogen associated with opportunistic infections of a wide range of species, including pigs and humans. The absence of a suitable vaccine makes it difficult to control SEZ infection. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been previously identified as an immunogenic protein using immunoproteomic techniques. In the present study, we confirmed that the sequence of GAPDH was highly conserved with other Streptococcus spp. The purified recombinant GAPDH could elicit a significant humoral antibody response in mice and confer significant protection against challenge with a lethal dose of SEZ. GAPDH could adhere to the Hep-2 cells, confirmed by flow cytometry, and inhibit adherence of SEZ to Hep-2 cells in an adherence inhibition assay. In addition, real-time PCR demonstrated that GAPDH was induced in vivo following infection of mice with SEZ. These suggest that GAPDH could play an important role in the pathogenesis of SEZ infection and could be a target for vaccination against SEZ.

• Journal of the Economic Geographical Society of Korea
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• v.19 no.1
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• pp.122-142
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• 2016

This paper examines the implications of North Korea's Special Economic Zone (SEZ) strategy since the early 1990s in terms of the shifts in both North Korea's economic system and geopolitical order on the Korean peninsula. Specifically, it analyzes the shifts in North Korea's SEZ policy in three different aspects-North Korea's unique territorial logic, stress on decentralization, and comparison with Chinese reform and opening-up. Based upon this analysis, this paper criticizes a linear approach to understand North Korea's economic transformation from isolation to opening-up, and explores the dynamics of North Korea SEZ strategy in various dimensions. It contends that North Korea's SEZ strategy is neither an inevitable choice from economic difficulty nor an adoption of Chinese model of reform and opening up. Rather this paper focuses on the geopolitical logic and local development imperatives underlying SEZ strategy.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.7
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• pp.1104-1110
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• 1999

We condicted two experiments to evaluate the interaction among fumaric acid (FA), protected acids (PA), or no additional acid (NO) and two different levels of acid buffering capacity (BC) in diets for 14-d-old weaned pigs. BC was varied substituting mono-calcium phosphate and calcium sulfate for dicalcium phosphate and calcium carbonate. In the high BC diet plus PA, FA was also added. In Exp. 1, 48 gilts were raised for 31 days on the six diets, evaluating growth performance and fecal digestibility. In Exp. 2, 42 gilts were raised. With each diet three subjects were sacrificed after 19 days and four after 38 days. In addition, six subjects were sacrificed at weaning. Growth and carcass performance, ileal digestibility, bacterial populations and pH in the gut were assessed. The piglet performance and stomach, ileal and cecal pH, and empty body composition were not affected by the diets. Empty body composition other than ash content was affected by piglet age (p<0.01). The BC did not influence digestibility. The dietary inclusion of PA improved fecal digestibility of protein (p<0.05) compared to the addition of FA and NO. Ileal digestibility slightly increased with both acid additions (p<0.10), the groups receiving PA showing the higher values. Piglets fed diets with low BC had lower Lactobacillus and E. coli counts in the ileum (p=0.07) and higher Lactobacillus in the colon (p=0.08). Acidified diets tended to reduce E. coli counts in the ileum (p=0.10) and increased Lactobacillus in the colon (p=0.09). The addition in the diet of PA increased Lactobacillus in the ileum compared to the sole addition of free fumaric acid (p=0.07). The addition of protected acids, combined with free fumaric acid in the case of high BC diets, increased protein digestibility and Lactobacillus counts and reduced E.coli counts. Only some changes in the concentration of bacterial population can be expected with a diet of low BC.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.337-345
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• 2014

Pichia pastoris is one of the most widely used expression systems for the secretory expression of recombinant proteins. The secretory expression in P. pastoris usually makes use of the prepro $MAT{\alpha}$ sequence from Saccharomyces cerevisiae, which has a dibasic amino acid cleavage site at the end of the signal sequence. This is efficiently processed by Kex2 protease, resulting in the secretion of high levels of proteins to the medium. However, the proteins that are having the internal accessible dibasic amino acids such as KR and RR in the coding region cannot be expressed using this signal sequence, as the protein will be fragmented. We have identified a new signal sequence of 18 amino acids from a P. pastoris protein that can secrete proteins to the medium efficiently. The PMT1-gene-inactivated P. pastoris strain secretes a ~30 kDa protein into the extracellular medium. We have identified this protein by determining its N-terminal amino acid sequence. The protein secreted has four DDDK concatameric internal repeats. This protein was not secreted in the wild-type P. pastoris under normal culture conditions. We show that the 18-amino-acid signal peptide at the N-terminal of this protein is useful for secretion of heterologous proteins in Pichia.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.7
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• pp.995-1002
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• 2000

To evaluate energy efficiency and partition of nutrients, 32 piglets were weaned at 14 d of age and individually fed diets containing 15% fat from coconut oil (CO, medium chain saturated), high oleate sunflower oil (HOSO, n-9 series), soybean oil (SO, n-6 series), or linseed oil plus fish oil, (LF, n-3 series). After 4 weeks, the subjects were sacrificed to evaluate empty body composition and apparent ileal digestibility with the slaughter method. No statistical effect of dietary fat sources on growth was observed. The digestibility of fat from the coconut oil diet was higher than fats from the diets containing high levels of unsaturated fatty acids. The efficiency of use of metabolizable energy for growth averaged 63% and was not affected by the diet. Dietary fat composition was reflected strongly in backfat. Total body neutral and polar fatty acids were influenced too. For the whole body phospholipid fraction the ratio of n-6 to n-3 and the double bond index were 4.3, 5.8, 7.2, 0.78 and 69, 87, 89, 87 for CO, HOSO, SO, and LF respectively. These results show that for the coconut oil diet the degree of unsaturation of phospholipids in the body was lower and that, in the other diets, it did not differ, but double bond index was maintained with different n-6 to n-3 ratios in carcass fat. On the whole the data on body fat composition indicate that the dietary fat tended to be deposited in similar quantity in the body, whatever was the dietary fatty acid profile.

• Korean Journal of Veterinary Research
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• v.47 no.4
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• pp.409-415
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• 2007

An eight years old female barbary sheep (Ammotragus lervia), which bred at the Gwangju Uchi Park Zoo had shown anorexia, depression, respiratory problem for several weeks after parturition. In necropsy, extensive necrotizing pneumonia was found with severe immunocytes infiltration in the alveolar spaces and bronchioles. Pulmonary pleura were thickened with fibrin and inflammatory cells. Bacteria were isolated from lung and identified as Streptococcus equi subsp. zooepidemicus (SEZ) by biochemical tests and PCR on sodA and gusA genes, though seel gene was not detected. Isolation of zoonotic SEZ in public place such as a zoo should be emphasized for the public health mangagement.

• Land and Housing Review
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• v.6 no.2
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• pp.79-88
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• 2015

North Korean leader Kim, Jung-Il visited Beijing, China, May 2010, when he made a common recognition with Chinese President Hu Jintao on construction of the Rasun SEZ and the Hwanggumpyong-Wyhwado SEZ for development of Economic cooperation between N.Korea and China and accelerating establishment of SEZs in N.Korea. However, after N.Korea's third nuclear test on Feb. 2013, the relationship between N.Korea and China became a little worse. Recently, three nations' border region near Rasun in N.Korea is reconsidered that it is very important place for collaboration between and among 2, 3 or 4 countries, S.Korea, N.Korea, China and Russia. This thesis examined these changes of cooperation and plans among the countries near the border region and proposed some measures for participation of S.Korea on the projects in the Rasun region.

• Journal of Contemporary Eastern Asia
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• v.20 no.2
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• pp.278-297
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• 2021

Despite competing strategical interests over Southeast Asia that have emerged in the last decade, with the launch of wide scope geopolitical strategies Chinese and Japanese initiatives have been characterized by a certain degree of implicit coordination, particularly in offering support to the Myanmar state's territorializing strategies for economic development. The case of the Thilawa Special Economic Zones (SEZ) is exemplary, as it was a Japan-led project which became a model and benchmark example for similar development initiatives supported by the People's Republic of China.

• KDI Journal of Economic Policy
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• v.37 no.sup
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• pp.87-121
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• 2015

To attract more FDI inflows, the Korean government has designated several special economic zones (SEZs), offering various advantages and support to the FDI. There is, however, a shared acknowledgement that those efforts have gained little reward. In this regard, this paper empirically analyzes company-level performances of labor productivity, operating profit ratio, propensity to invest and innovate, etc. and then conducts regression analysis and PSM analysis to see whether these performances are meaningfully different between foreign-invested firm and domestic firm and between foreign-invested firms. The main findings of this paper are as follows. First, in the aspects of labor productivity and operating profit ratio, no empirical evidence was found to support the hypothesis that foreign-invested firm outperforms domestic firm in efficiency and profitability, Second, in the aspects of propensity to invest, foreign-invested firms in foreign investment zones outperformed domestic firms. Third, in the aspect of R&D investment, overall, foreign-invested firms showed a stronger propensity to invest than domestic firms, but there is no empirical evidence that high propensity to invest was driven by the policy on special economic zones. In the aspect of investment in educational training, empirical evidences were found that the role of foreign-invested firms outside the special zones turned out to be the strongest and that among firms inside special zones, it was those in the free economic zone that outperformed domestic firms. Lastly, foreign-invested firms showed a stronger propensity to employ than domestic firms, but there is no empirical evidence that high propensity to employ was driven by the policy on special economic zones.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.271-281
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• 1999

The cost of production of high quality pork is compensated by high returns, but constraints by the market are increasing. A few opportunities to maintain and improve pork quality by dietary means are presented. The healthy value of pork is a prerequisite. A careful control of suppliers and preservation of feeds are essential to protect pork against presence of contaminants from the feed. The feeding level and some dietary components modify the partition of the dietary energy into different pig tissues and chemical components of pork, affecting the hygienic and nutritional value, tenderness and taste. It is difficult to transfer a nutrient from the diet to pork, if the requirements. for growth are satisfied. Fatty acids and Vitamin E are the most studied exceptions. There is some evidence that iron and selenium contents can be affected too. Varying the content of a nutrient frequently changes sensorial and technological properties of pork. The addition of oils improves the acidic profile of depot fats, but the effect on phospholipid composition is not well studied and negative effects on oxidability and consistency of meat products are observed. Vitamin E can improve many healthy and sensorial characteristics, but its effect is clearer when the meat is stressed or manipulated.