• Korean Journal of Agricultural Science
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• v.12 no.2
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• pp.242-252
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• 1985

In order to get information on the ecology of rice false smut, germination ability and pathogenicity of sclerotia and chlamydospores of the pathogen, environmental conditions affecting the disease outbreak and varietal resistance have been investigated. 1. The degree of outbreak of rice false smut was higher in the upland rice in comparison with the paddy field rice in respect to the number of affected grains per ear, the size and weight of smut balls formed on affected grains as well as the ratio of sclerotial formation produced on smut balls. 2. Germination percentage and days required for germination of overwintered sclerotia placed on the soil surface in July were 81% and 19 days, respectively, while those of overwintered sclerotia treated in May were 60-70% and 41 days. Sclerotia placed on the soil surface or under 1 cm depth of the soil surface and incubated at $25-30^{\circ}C$ were germinated well, whereas those placed under 3 cm or 5 cm depth of the soil surface were not germinated at all. Germinability and stroma productivity of sclerotia were reduced when the sclerotia were cutted into small pieces. 3. The average number of stroma formed on a sclerotium was six and that of perithecia formed in a stroma was about 50 to 140. 4. Percentage of germination of chlamydospores on the yellow balls was very high and was decreased as the color of the balls being darken with maturation. 5. Panicle of rice plants were successfully infected by injection inoculation with suspention of ascospores and chlamydospores of the pathogen to the sheaths at the booting stages, while seeding infection by spraying with suspensions of chlamydospores was unsuccessful. 6. More number of infected grains was distributed on basal parts of an affected ear than that of infected ones distributed upper parts of the ear, when the affected ear was divided into five parts from its basal portion to the apical of the ear. 7. The occurrence of the disease was more severe in the late maturing varieties of rice in comparison with the early maturing varieties. 8. When the level of nitrogen applied was increased, the incidence of disease increased, and the infection percentage of the disease was increased as the transplanting date was delayed. 9. The weight of panicles and 1000 kernels and the ratio of ripenness were reduced, and the contamination degree of grains with chlamydospores were increased as the number of smut balls per panicle were increased.

• Korean Journal of Remote Sensing
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• v.33 no.5_2
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• pp.699-708
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• 2017

The present study was to develop an approach for predicting soybean yield using a crop growth simulation model at the regional level where the detailed and site-specific information on cultivation management practices is not easily accessible for model input. CROPGRO-Soybean model included in Decision Support System for Agrotechnology Transfer (DSSAT) was employed for this study, and Illinois which is a major soybean production region of USA was selected as a study region. As a first step to predict soybean yield of Illinois using CROPGRO-Soybean model, genetic coefficients representative for each soybean maturity group (MG I~VI) were estimated through sowing date experiments using domestic and foreign cultivars with diverse maturity in Seoul National University Farm ($37.27^{\circ}N$, $126.99^{\circ}E$) for two years. The model using the representative genetic coefficients simulated the developmental stages of cultivars within each maturity group fairly well. Soybean yields for the grids of $10km{\times}10km$ in Illinois state were simulated from 2,000 to 2,011 with weather data under 18 simulation conditions including the combinations of three maturity groups, three seeding dates and two irrigation regimes. Planting dates and maturity groups were assigned differently to the three sub-regions divided longitudinally. The yearly state yields that were estimated by averaging all the grid yields simulated under non-irrigated and fully-Irrigated conditions showed a big difference from the statistical yields and did not explain the annual trend of yield increase due to the improved cultivation technologies. Using the grain yield data of 9 agricultural districts in Illinois observed and estimated from the simulated grid yield under 18 simulation conditions, a multiple regression model was constructed to estimate soybean yield at agricultural district level. In this model a year variable was also added to reflect the yearly yield trend. This model explained the yearly and district yield variation fairly well with a determination coefficients of $R^2=0.61$ (n = 108). Yearly state yields which were calculated by weighting the model-estimated yearly average agricultural district yield by the cultivation area of each agricultural district showed very close correspondence ($R^2=0.80$) to the yearly statistical state yields. Furthermore, the model predicted state yield fairly well in 2012 in which data were not used for the model construction and severe yield reduction was recorded due to drought.

• Korean Journal of Soil Science and Fertilizer
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• v.27 no.2
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• pp.125-135
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• 1994

Field experiment was carried out in order to clarify effects of plastic film mulching on temperature and moisture of rhizosphere soil and their subsequent effects on seedling emergence, earlier growth, vegetative growth and grain yield of sesame. The textural class of the experimental field soil was the sandy loam(Bonyang series) and the variety of sesame planted was "Ansan-ggae". The experiment was conducted by combining four sowing dates of April 25, May 10, May 25, June 10 and two mulching treatments(mulching, non-mulching) over two year of 1991 and 1992. The results were summarized as follows : 1. The daily mean soil temperature of 5cm deep soil was increased by 1.4 to $2.8^{\circ}C$ by plastic film mulching. The average soil water content was increased by 0.5 to 3.0%(V/V) in the drier season, while decreased by 1.0 to 2.0%(V/V) in the rainy season by mulching. 2. The establishment rate of sesame seedling was very sensitive to soil temperature. For normal seedling emergence, from the seeding date to the 7th date after sowing, the daily mean soil temperature higher than $21.0^{\circ}C$ was required at the experimental field conditions. 3. The average soil water content in the range of 14.0 to 21.0%(V/V) at 5cm deep soil seemed not to be limiting for the germination and emergence of sesame. The effect of soil water content on seedling establishment was very small in this range, but the optimum level of soil water content ranged from 14.0 to 15.0%(V/V) in the experimented sandy loam. 4. The wetter the soil profile was, the larger the gap of soil temperature between the mulched and non-mulched condition was. The effect of mulching on the establishment rate of sesame seedlings was much greater in the lower air temperature conditions. However, when the sowing of sesame came earlier than at the date with the daily mean air temperature below $19.0^{\circ}C$, the effects of earlier sowing and mulching were offsetted by the retarded seedling growth due to the low air temperature, and thus earlier sowing with mulching did not enhance the grain yield of sesame.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.300-307
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• 2015

In order to develop optimum paddy-upland rotation system, we evaluated the 1st and the 2nd upland growth and yield characteristics of foxtail millet, proso millet, sorghum rotated from paddy field and rice rotated from upland in paddy-upland rotation. Average number of ears per hill was 3.3 in the 2nd upland cultivation. The value was greater by 1 ear as compared to 1st upland cultivation (2.2 ears per hill). In average yield per 10a, the 2nd upland cultivation showed 220.3 kg, 23% increased yield compared to the 1st upland cultivation (179 kg per 10a). In average number of ears per hill, the 2nd upland cultivation showed 8.3 ears, increased 4 ears compared to the 1st upland cultivation (4.2 ears per hill). In average yield per 10a, the 2nd upland cultivation showed 152.8 kg, 16.8% increased yield compared to the 1st upland cultivation (130.8 kg per 10a). In average days from seeding to heading of 5 sorghum varieties, there were no significant difference between the 1st (68.6 days) and the 2nd (67.4 days) upland cultivation rotated from paddy field. In the average number of grains per ears, the 2nd upland cultivation showed 2,931.6 grains per ear, 12% increased compared to the 1st upland cultivation (2,619.6 grains per ears). Average yield per 10a of sorghum in the 2nd upland cultivation showed 242.3 kg, 4.6% increased compared to the 1st upland cultivation (231.7 kg per 10a). In growth and yield characteristics of rice in paddy-upland rotation, culm length in paddy-upland-paddy plot showed 82.9 cm, 7.3 cm longer compared to the continuous rice paddy field (75.6 cm). Ear length was also 1 cm longer than that of the continuous rice paddy field. In average number of ears per hill, paddy-upland-paddy plot showed 25.0 ears, 4.3 ears more than that of the continuous rice paddy field (20.7 ears per hill). In average yield of rice per 10a, the paddy-upland-paddy rotation plot showed 526.8 kg, 9.8% higher yield compared to the continuous rice paddy field (479.9 kg per 10a).

• Radiation Oncology Journal
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• v.19 no.2
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• pp.163-170
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• 2001

Purpose : The measurement of radiation survival using a clonogenic assay, the established standard, can be difficult and time consuming. In this study, We have used the MTT assay, based on the reduction of a tetrazolium salt to a purple formazan precipitate by living cells, as a substitution for clonogenic assay and have examined the optimal condition for performing this assay in determination of radiation sensitivity. Materials and Methods : Four human cancer cell lines - PCI-1, SNU-1066, NCI-H630 and RKO cells have been used. For each cell line, a clonogenic assay and a MTT assay using Premix WST-1 solution, which is one of the tetrazolium salts and does not require washing or solubilization of the precipitate were carried out after irradiation of 0, 2, 4, 6, 8, 10 Gy. For clonogenic assay, cells in $25\;cm^2$ flasks were irradiated after overnight incubation and the resultant colonies containing more than 50 cells were scored after culturing the cells for $10\~14$ days. For MTT assay, the relationship between absorbance and cell number, optimal seeding cell number, and optimal timing of assay was determined. Then, MTT assay was performed when the irradiated cells had regained exponential growth or when the non-irradiated cells had undergone four or more doubling times. Results : There was minimal variation in the values gained from these two methods with the standard deviation generally less than $5\%$, and there were no statistically significant differences between two methods according to t-test in low radiation dose (below 6 Gy). The regression analyses showed high linear correlation with the $R^2$ value of $0.975\~0.992$ between data from the two different methods. The optimal cell numbers for MTT assay were found to be dependent on plating efficiency of used cell line. Less than 300 cells/well were appropriate for cells with high plating efficiency (more than $30\%$). For cells with low plating efficiency (less than $30\%$), 500 cells/well or more were appropriate for assay. The optimal time for MTT assay was after 6 doubling times for the results compatible with those of clonogenic assay, at least after 4 doubling times was required for valid results. In consideration of practical limits of assay (12 days, in this study) cells with doubling time more than 3 days were inappropriate for application. Conclusion : In conclusion, it is found that MTT assay can successfully replace clonogenic assay of tested cancer cell lines after irradiation only if MTT assay was undertaken with optimal assay conditions that included plating efficiency of each cell line and doubling time at least.

• Korean Journal of Environmental Agriculture
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• v.19 no.4
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• pp.358-363
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• 2000

This study was carried out to obtain the basic data for selecting the applicable crops in reclaimed land during desalinization period. A pot experiment was conducted with 5 different electrical conductivities of the saturated extracts $(ECe\;1,\;3,\;9,\;14,\;and\;16\;dS{\cdot}m^{-1})$ of soils taken from the Dae-Ho reclaimed tidal lands. Eight crops (Chinese cabbage, radish, tomato, red pepper, buckwheat, soybean, sesame, and green perilla) were grown for 37days. Plant height and number of leaves were surveyed on 2 and 4 weeks after seeding, and on harvest time (5 weeks). After harvest, dry weights of harvested crops were measured and soil chemical properties were analyzed. Emergence rates of crops were comparatively high except sesame. For sesame, there was no emergence at ECe over $3\;dS{\cdot}m^{-1}$. Growth and dry weight decreased significantly as increasing ECe. The ECe which decreased 50% of dry weight index were $14.2\;dS{\cdot}m^{-1}$ for radish, $11.4\;dS{\cdot}m^{-1}$ for Chinese cabbage, $10.2\;dS{\cdot}m^{-1}$ for red pepper, $8.9\;dS{\cdot}m^{-1}$ for buckwheat and green perilla, $8.6\;dS{\cdot}m^{-1}$ for soybean, and $8.9\;dS{\cdot}m^{-1}$ for tomato. At higher ECe that start the growth inhibition, increasing $1\;dS{\cdot}m^{-1}$ in ECe, 7.7, 6.5, 5.9, 5.6, 5.2, and 4.9% of dry weight decreased for buckwheat, green perilla, Chinese cabbage, radish, soybean, and tomato (red pepper), respectively. The critical value of ECe for crop survival except sesame was $15.4\;{\sim}\;23.1\;dS{\cdot}m^{-1}$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.52-57
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• 2006

This research was carried out to determine the effect of controlling nitrogen application on tiller development, yield, and quality of rice under scanty or excess seedling stands in direct-seeded rice on flooded soil surface. Seedling stand was set to 3 levels: scanty $(60/m^2)$, optimum $(120/m^2)$, and excess $(200/m^2)$. In the scanty plot, additional 4 kg/10a nitrogen was applied at the 3rd leaf stage to promote tiller development. On the other hand, applying 3.3 kg/10a nitrogen at 5th leaf stage in the excess plot was omitted to suppress tillering. Maximum numbers of tillers per plant were 15.2 and 8.6 in scanty and excess plots, respectively, as compared with 9.8 in optimum plot. Productive tiller rate decreased with the increase in number of seedling stands. Regardless of seedling density, the first tiller developed on the 3rd node of rice stem from the bottom. The primary tillers developed at 3, 4, 5, 6, 7 nodes in scanty plot, 3, 4, 5 nodes in optimum plot, and 3, 4 nodes in excess plot. The secondary tillers developed only in some portion of plants in scanty and optimum plots. The order of tiller emergence was negatively correlated to stem length, panicle length, non-productive tiller number, grain number per panicle, and fertility in scanty plot, and to perfect grain ratio in excess plot. In the optimum plot, however, the order of tiller emergence was not correlated to any of the mentioned characteristics. The perfect grain ratio of scanty plot was the highest because green-kerneled rice was a very small portion in the primary tillers as compared with those of optimum and excess plots. Yield indexes of scanty and excess plots were 99%, and 97%, respectively, of the yield (494 kg/10a) in optimum plot. In conclusion, when seedling stands are not at optimum level, rice yield and quality similar to optimum planting density level can be obtained by means of controlling nitrogen application.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.166-176
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• 2015

Research was initiated to investigate root growth characteristics of major cool-season grasses (CSG) and to collect basic information useful for sports turf design, construction and maintenance. Several turfgrasses were evaluated in the USGA (United States Golf Association) soil system. Turfgrass entries were comprised 3 blends and 3 mixtures of Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), and tall fescue (TF, Festuca arundinacea Schreb.). Significant differences were found in root growth, rooting potential and rooting development. These characteristics increased with time after seeding, but varied with establishment stages. In early stage, root length was highest with PR, intermediate with TF and lowest with KB. Evaluation in a middle stage indicated that root growth was similar to early-stage evaluation, but decreased by 13 to 31% compared with early-stage values. Root growth of late stage increased by 34 to 85% over middle-stage root growth. Overall, thhere was not much difference in root length among treatments, with all except Mixture I reaching 22cm in root length. Rooting potential ranking was variable with establishment stage, being PR > KB > TF in early stage, PR > TF > KB in middle stage and TF > PR > KB in late stage. At the end of the study, TF was rated best for rooting development, followed by PR and finally KB. Our results showed that TF was the best species in regard to overall rooting characteristics. TF exhibited excellent rooting development with time after establishment. Bunch-type PR showed fast root growth in the early stage, but rooting quality characteristics decreased with time, especially for rooting development. By contrast, rhizomatous-type KB was poor in early-stage root growth, but rooting characteristics improved with time after establishment. These variations in rooting characteristics among CSGs were considered to arise from differences in establishment vigor, growth habit and genetic characteristics. Information on root growth, rooting potential and rooting development by establishment stages will be useful for sports turf design, construction and maintenance.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.4
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• pp.256-262
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• 2003

The emission of methane and nitrous oxide was measured from fields of upland rice and soybean. Rice cultivar, Daejinbyeo, was seeded in a row of 30 cm space in upland soil on April 28, 2001. The fields were irrigated with sprinkler irrigation or natural precipitation. Three soybean cultivars Jangyeobkong, Taekwangkong and Hwangkeumkong, were sown with seeding space of $60{\times}15cm$ on June 16, 2001. Gas samples were collected twice a week during the growth period, and methane and nitrous oxide were analyzed. Methane emission from upland rice field of sprinkler irrigation was $3.5kg\;CH_4\;ha^{-1}$. However, negative emission of methane, $-1.7kg\;CH_4\;ha^{-1}$, was observed in upland rice field of natural precipitation. Total nitrous oxide emission from upland rice field of sprinkler irrigation was $50.8kg\;N_2O\;ha^{-1}$, while the emission in the field of natural precipitation was $8.3kg\;N_2O\;ha^{-1}$. Global warming potentials (GWP) in the upland rice fields of sprinkler irrigation and natural precipitation were 15,822 and $2,216kg\;CO_2\;ha^{-1}$, respectively. Methane emissions from fields of soybean cultivars Jangyeobkong, Taekwangkong and Hwangkeumkong were -14.7, -4.3 and $4.8kg\;CH_4\;ha^{-1}$, respectively. Total nitrous oxide emissions from fields of soybean cultivars Jangyeobkong, Taekwangkong and Hwangkeumkong were 4.7, 4.6 and $3.0kg\;N_2O\;ha^{-1}$, respectively. Total GWPs for soybean cultivars Jangyeobkong, Taekwangkong and Hwangkeumkong were 1152, 1323 and $1027kg\;CO_2\;ha^{-1}$, respectively.

• Korean Journal of Weed Science
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• v.3 no.1
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• pp.29-38
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• 1983

Growth habit, intraspecific competition of Digitaria sanguinalis, and interspecific competition between soybean and D. sanguinalis were evaluated to obtain the basic informations on establishing an effective control measure in upland corps. The earlier planting on April 1 produced approximately 3.5 times higher dry matter than that of the latest planting on July 22 and resulted in the earliest heading on July 8 and the latest heading was observed on September 4 planted on July. 22, but the interval between planting and heading dates was shortened progressively as the planting dates were delayed, showing irregularity of heading within a hill. Tiller numbers per plant were inhibited as densities increased from one to forty. One plant planted per pot produced significantly higher tiller numbers than density of 5 to 40 plants per pot. However, total tiller numbers was the highest in density of 40 plants planted per pot. Regardless of planting densities used, D. sanguinalis at all densities produced the similar dry matter per pot, showing severe intraspecific competition as density increased, but on each plant basis dry weight production was the highest in the lowest density such as one plant planted per pot. Competition between soybean and D. sanguinalis for the entire growing season decreased the total dry weight of soybean by 59.6%. Soybean required the maintenance of a weed free condition, about 3 to 4 weeks immediately after seeding, for obtaining the maximum yield, beyond which soybean crops effectively suppressed the growth of D. sanguinalis.