• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.94-100
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• 2010

Zymomonas mobilis was immobilized in a modified graphite felt cathode with neutral red (NR-cathode) and Saccharomyces cerevisiae was cultivated on a platinum plate anode. An electrochemical redox reaction was induced by 3 volts of electric potential charged to the cathode and anode. The Z. mobilis produced 1.3-1.5 M of ethanol in the cathode compartment, whereas the S. cerevisiae produced 1.7-1.9 M in the anode compartment after 96 h. The ethanol produced by the Z. mobilis immobilized in the NR-cathode and S. cerevisiae cultivated on the platinum plate was 1.5-1.6 times higher than that produced under conventional conditions. The electrochemical oxidation potential inhibited Z. mobilis, but activated S. cerevisiae. The SDS-PAGE pattern of the total soluble proteins extracted from the Z. mobilis cultivated under the electrochemical oxidation conditions was gradually simplified in proportion to the potential intensity. Z. mobilis and S. cerevisiae were cultivated in the cathode and anode compartments, respectively, of an electrochemical redox combination system. The Z. mobilis culture cultivated in the cathode compartment for 24 h was continuously transferred to the S. cerevisiae culture in the anode compartment at a rate of 300 ml/day. Approx. 1.0-1.2 M of ethanol was produced by the Z. mobilis in the cathode compartment within 24 h, and an additional 0.8-0.9 M produced by the S. cerevisiae in the anode compartment within another 24 h. Thus, a total of 2.0-2.1 M of ethanol was produced by the electrochemical redox combination of Z. mobilis and S. cerevisiae within 48 h.

• Current Research on Agriculture and Life Sciences
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• v.8
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• pp.9-18
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• 1990

Germinating ability of 54 weed species collected throughout Korea in 1987 and 1988 was determined in incubator at $25^{\circ}C$ for 15 days. The seeds of Echinochloa sp, collected from 70 different places throughout Korea, were classified by Yabuno's seed morphology method. Plant characters of two identified Echinochloa species, one having two subspecies were evaluated. SDS-PAGE(sodium dodecyl sulfate polyacrylamide gel electrophoresis) of seed protein assay was used to identify locally collected Echinochloa species classified by Yabuno's seed morphology method. 1. Out of 54 weed species collected, 31 species showed germination at 15 days after incubation, and 14 species showed over 50% and no germination was observed in 24 species, indicating that approximately 40 species had varied degree of germinating ability. 2. It was confirmed that two annual species of Echinochloa such as E. oryzicola and E. crus-galli occurred in Korea and one of which particularly E. crus-galli has two subspecies such as E. c. var. praticola and E. c. var. crus-galli. 3. E. oryzicola was distinguished from E. c. var crus-galli and E. c. var praticola by presence or absence of one major protein band in B zone. However, it was rather difficult to distinguish E. crus-galli species because they showed similar protein pattern.

• Food Science of Animal Resources
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• v.25 no.4
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• pp.423-429
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• 2005

Properties of pant surimi derived from porcine longissimus muscle were investigated Rapid glycolysis of muscle reduced yield $\%$ of water-washed pork and moisture $\%$ of pent surimi because of ie lower ultimate pH. Gel Hardness was significantly (p<0.05) higher in pork surimi from rapid glycolysis muscle, but springiness was higher (p<0.05) in pork surimi from normal glycolysis muscle. SDS-PAGE pattern showed denaturation of sarcoplasmic proteins onto myofibrillar proteins in rapid glycolysis muscle, resulted in dark color and hard texture of pork surimi. Color and texture of gels were related with water-holding capacity of muscle proteins and moisture $\%$ in gel matrix. Results imply that glycolysis rate of porcine muscle at postmortem could affect gel properties of pork surimi, and muscle with rapid glycolysis muscle could produce a hard texture of pork surimi and dark color.

• Journal of Aquaculture
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• v.4 no.1
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• pp.67-71
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• 1991

Sensitivity to ethylmethanesulphonate(EMS) on Ulva Pertusa Kjellman thalli was investigated as preliminary studies aimed at mutation breeding of seaweeds. When the thalli were grown in medium containing the mutagen no growth and about $20{\%}$ growth inhibition occurred at $0.05{\%}$ and $0.025{\%}$ EMS-mixed medium respectively. In the case the thalli were treated short time at high concentration mutagen solution followed by culture in control medium no growth and about $10{\%}$ growth inhibition occurred at 40 min. in $1.0{\%}$ and 80 min. in $0.5{\%}$ EMS solution respectively. Among the mutagenized tissues, some showed variation in growth from and cell color contrast to the mother tissue. The mutagenized tissue polypeptide pattern using sodium dodecyl sulphate-polyacrylamide gel electrophoresis was simiiar that of the mother tissue except about 6.6 kD polypeptides.

• Journal of Veterinary Clinics
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• v.25 no.5
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• pp.317-323
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• 2008

Canine trypsin-like immunoreactivity (cTLI), which is a mirror of the concentration of trypsin and trypsinogen, is a pancreas-specific enzyme and a suitable marker for canine pancreatitis and especially exocrine pancreatic insufficiency (EPI). To develop the immunochromatographic test kit, monoclonal antibodies that recognize cTLI were prepared. Anionic trypsin, cationic trypsin, and chymotrypsin from canine pancreas were successfully purified to homogeneity, using ammonium sulfate fractionation and benzamidine-affinity chromatography. The purification fold for anionic trypsin was 108 times when compared with that of the homogenation of pancreas. The molecular weights by SDS-PAGE analysis were approximately 23 kDa for chymotrypsin and approximately 20 kDa for cationic trypsin and anionic trypsin, respectively. Using the purified trypsin-like proteins, ten hybridomas which secret canine trypsin-specific monoclonal antibody were prepared. Klotz plot indicated that hybridomas, 5G2H10G4 and 2F4A11, have high affinity constant (Ka) of $4.1\;{\times}\;10^{9}$ and $1.8\;{\times}\;10^{9}$, respectively. Especially, 5F9H3 showed the cationic typsin-specific binding pattern and its Ka was determined to $4.5\;{\times}\;10^{9}$. The development of immunochromatographic test kit using these monoclonal antibodies against cTLI will be very useful in the diagnosis of canine EPI or canine pancreatitis.

• Food Science of Animal Resources
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• v.32 no.2
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• pp.162-167
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• 2012

Phosvitin was extracted from a chicken egg yolk and the iron-binding, along with antioxidative activity of the extracted phosvitin, was determined after mixing with ground beef at the concentrations of 100 and 500 mg/kg of meat. The electrophoretic pattern of the extracted phosvitin on SDS-PAGE was found to be identical to that of the standard phosvitin. The extracted phosvitin at $1,000{\mu}g$/mL showed an ability to bind approximately 65% of the iron in a 3 mM iron solution. Lipid oxidation was inhibited in the ground beef mixed with 500 mg/kg of the extracted phosvitin, during storage at $4^{\circ}C$ compared to that of the control (p<0.05). Additionally, color stability of ground beef containing the extracted phosvitin was enhanced (p<0.05). The pH, cooking loss, texture, and sensory properties of the ground beef were not affected, by adding up to 500 mg/kg of the extracted phosvitin. This result suggests that the phosvitin extracted from egg yolk could be used as an antioxidant reagent. In particular, phosvitin would be more amenable for use in meat products because it is a natural protein derived from animal products.

• Korean Journal of Food Science and Technology
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• v.22 no.1
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• pp.50-55
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• 1990

Differences in the major components between non-greening and greening garlic bulbs were compared, and the several factors to be affected on the greening were investigated to study on the greening phenomenon occured in garlic puree made from stored garlic bulbs. From the several chemical analyses it was found that the contents of total nitrogen and lipid were lower in the greening than in the non-greening bulb but the contents of pyruvic and succinic acid were not. The content ratio of glutamic acid to total amino acid and the composition ratios of linoleic and linolenic acid were high in the greening bulb in comparsion with the non-greening. The protein pattern of the greening bulb by SDS-PAGE was significantly differed from that of the non-greening, especially in molecular weight ranges of 45.0-66.2 Kd. From the studies on the effects of pH, temperature and duration on the development of green color in the garlic powder solution, the optimum pH for the greening was 5.5 and maximum degree of the greening in the pH adjusted solution was shown after 30 min at $50^{\circ}C$.

• The Korean Journal of Ecology
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• v.16 no.2
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• pp.135-149
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• 1993

In order to study and ecotypic variation of Phragmites communis Trin., we investigated germination rates, velocities, and protein band patte군 of seeds of three population of salt marsh, estuary and fresh water areas of Muan Peninsular in southwestern coast of Korea from March, 1990 to October, 1992. The highest germination rates of seeds were observed at $25^{\circ}C$; those of population of the estuary and fresh water are were 100% and that of the salt marsh was 95%. Similar germination rates were observed from the populations of estuary and fresh water areas at $30^{\circ}C$ and $20^{\circ}C$, but they decreased at $15^{\circ}C$. The onset of germination of seeds of three population was earlier $^{\circ}C$, but they decreased at $15^{\circ}C$. The onset of germination of seeds of th three populations was earlier at both $25^{\circ}C$, which was higher than those of any other areas, while that of fresh water areas was the lowest. were different; those of salt marsh and estuary decreased to 30% and 2.5%, respectively, at 3.0% of salt content, but seeds of the fresh water area did not germinate at all at the same salt content. The onset of germination was delayed in the order of the salt marsh, esturay and fresh water areas as salt content of culture solution increased. Germination of seeds from the population of salt marsh was found to begin earliest. The highest germination velocity of three populations was observed in the culture containing no salt. The germination velocity constant decreased as salt content of culture solution increased from 0.5% to 3.0%: those of the populations of the salt marsh, estuary, and fresh water areas were 9.50, 0.75 and 0.00, respectively, at the salt concentration of 3.0%. Soluble protein patterns of seedings from the three populations were analyzed by SDS-PAGE method. The results showed that protein patterns of the three populations were distinctly different qualitatively and quantitatively. The present study suggests that populations of Phragmites communis Trin. in the coast of Korea had taken ecotypic variations of habitats, i.e., fresh water, estuary, and salt marsh types, according to the salt content.

• Applied Biological Chemistry
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• v.51 no.3
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• pp.159-163
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• 2008

Xylogone sphaerospora ${\beta}$-mannanase was purified by Sephadex G-100 column chromatography. The specific activity of the purified enzyme was 8.44 units/ml protein, representing an 56.27-folds purification of the original crude extract. The final preparation thus obtained showed a single band on SDS-polyacrylamide gel electrophoresis. The molecular weight was determined to be 42kDa. Konjac glucomannan was hydrolyzed by the purified ${\beta}$-mannanase, and then the hydrolysates was separated by activated carbon column chromatography. The main hydrolysates were composed of D.P. (Degree of Polymerization) 3 and 4 glucomannooligosaccharides. For elucidate the structure of D.P 3 and 4 glucomannooligosaccharides, sequential enzymatic action was performed. D.P 3 and 4 were identified as M-G-M and M-M-G-M (G- and M- represent glucosidic and mannosidic link-ages). To investigate the effects of konjac glucomannooligosaccharides on in vitro growth of Bifido-bacterium longum, B. bifidum, B. infantis, B. adolescentis, B. animalis, B. auglutum and B. breve. Bifidobacterium spp. were cultivated individually on the modified-MRS medium containing carbon source such as D.P. 3 and D.P. 4 glucomannooligosaccharides, respectively. B. longum and B. bifidum grew up 3.9-fold and 2.8-fold more effectively by the treatment of D.P. 4 glucomannooligosaccharides, compared to those of standard MRS medium. Especially, D.P. 4 was more effective than D.P. 3 glucomannooligosaccharide on the growth of Bifidobacterium spp.

• Korean Journal of Weed Science
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• v.12 no.4
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• pp.368-373
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• 1992

The effect of alachlor treatment on protein synthesis was studied. Protein synthesis was inhibited by $1{\times}10^{-4}$ M and $1{\times}10^{-3}$M of alachlor 5.8% and 86.5%, respectively, while did not occur blow $1{\times}10^{-5}$M alachlor. Soluble protein of alachlor treated oat root tips was examined by polyacrylamide gel electrophoresis. The proteins extracted from oat root tips showed that they were made up of subunits blow 100 kd polypeptides by SDS-PAGE. As compared to control, high molecular proteins(above 47 kd) were inhibited of oat root treated with alachlor, while low molecular proteins(below 23 kd) were increased. Two-D gels showed that alachlor caused decrease(1-6 spots) or increase(7-10 spots) in number of polypeptides on silver staining. The intensity of some polypeptides of soluble proteins (molecular mass of 83 kd : 1, 2 spots, 70 kd : 3, 4 spots, and 47.5 kd : 5, 6 spots) decreased in alachlor treatment, whereas the intensity of other peptide bands (20 kd : 7 spot and 16 kd : 8, 9, 10 spots) increased. Oat root tip proteins present in the neutral zone are masked by diffusing of major proteins, but proteins in acid zone are resolved minor proteins.