• Korean Journal of Microbiology
• /
• v.26 no.1
• /
• pp.37-43
• /
• 1988

This research was focused on investigation of the condition for protoplast formation and regeneration of protoplast fusion between Saccharomyces cerevisiae which has fermentation ability and Candida cariosilignicola which can grow at high temperature and utilize methanol. The results obtained were as follows; The highest production was collected in exponential growth phase. Ninety-nine% protoplast formation of C. cariosilignicola was obtained in glycin-NaOH buffer (pH10.0) containing Zymolyase 0.5mg/ml at $35^{\circ}C$ for 1hr incubation. The highest regeneration was produced when protoplast wuwpension containing 0.5% soft agar in buffered 50mM $CaCl_{2}$ was poured as a soft overlay onto 2% agar plates. Equal amuont of protoplast suspension of two strains was mixed and centrifuged. The subsequent pellet was added to 2ml of 35% polyethylene glycol (MW 4,000) containing 50mM $CaCl_{2}$, and incubated at $30^{\circ}C$ for 10min. Then 0.1ml of the suspension of aggregated protoplast was immediately covered with minimal medium and incubated at $40^{\circ}C$ for 5-7 days. As results, $SC_{1}$, $SC_{2}$, and $SC_{3}$ fusants were obtained. The physiological characteristics of fusants produced by protoplast fusion were; $SC_{1}$, and $SC_{2}$ utilized maltose, galactose, methanol, potassium nitrate. $SC_{3}$ utilized all the above materials except galactose.

• Journal of Applied Biological Chemistry
• /
• v.52 no.4
• /
• pp.157-162
• /
• 2009

An agar-liquefying bacteria (SC-22), which produces a diffusible agarase that caused agar softening around the colony was isolated from Daecheong lake in Korea. Chemotaxanomic and phylogenetic analyses based on 16S rRNA gene sequences revealed the strain was classified as Cellvibrio mixtus SC-22. The isolate SC-22 showed maximal extracellular agarase activity with 58.5 U/mL after 48 h cultivation in the presence of 0.2% agar. It was observed that the isolate produced two kinds of extracellular and three kinds of intracellular isoenzymes. The major agarase was purified from the culture filtrate of agarolytic bacteria by ammonium sulfate precipitation, anion exchange and gel filtration column chromatographic methods. The molecular mass of the purified enzyme was estimated to be 25 kDa by SDS-PAGE. The optimum pH and temperature of the purified enzyme were pH 7.0 and $50^{\circ}C$, respectively. The agarase activity was activated by $Fe^{2+}$, $Na^+$ and $Ca^{2+}$ ions while it was inhibited by $Hg^{2+}$, $Mn^{2+}$ and $Cu^{2+}$ at 1 mM concentration. The predominant hydrolysis product of agarose by the enzyme was galactose and disaccharide on TLC, indicating the cleavage of $\beta$-1,4 linkage in a random manner. The enzyme showed high substrate specificity for only agar and agarose among various polysaccharides.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.22 no.5
• /
• pp.787-793
• /
• 2018

In this paper, we propose a new single carrier - frequency domain equalization (SC-FDE) structure to cope with narrow band interference. In the conventional SC-FDE structure, when a high-power narrow band interference exists, channel estimation and data recovery is difficult. To relieve from this problem, this paper proposes a new SC-FDE frame structure to enable frequency-domain channel estimation in the environments that exist narrow band interference. Specifically, in the conventional method, the channel estimation is performed in time-domain first and from that, the frequency-domain channel is obtained by Fourier transform. In contrast, we proposed a new SC-FDE structure to enable frequency-domain channel estimation directly from received signals without time-domain channel estimation. The receiver performance improvement is verified through computer simulation. According to the results, the proposed technique can detect the signal with less than 2 dB loss compared with jammer-free environments, while the conventional method does not communicate with each other.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.15 no.9
• /
• pp.5776-5782
• /
• 2014

Relay-assisted wireless communication systems have been studied widely to cope with shadow areas and extend the cell coverage. This paper proposes a space-frequency (SF) block coded single carrier-frequency division multiple access (SC-FDMA) transmission system in a relaying multi-path shadow area and present the performance comparison of SC-FDMA systems based on the signal-to-noise power ratio (SNR) between a relay and a destination station. The performance of relaying SC-FDMA systems can be improved by applying SF block code to the recovered signals of relays before re-transmitting them. The simulation result showed that the SNR performance of the proposed SF block coded relaying SC-FDMA system was approximately 5 dB better than the SNR performance of the single-path relaying SC-FDMA system at a symbol error rate (SER) of $10^{-2}$.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.35 no.2
• /
• pp.265-274
• /
• 2015

The structural safety of nuclear power plant against impact from aircraft crash has been performed so far in two viewpoints such as local behavior and global behavior, and the local behavior has been evaluated using local damage evaluation formulas suggested based on the results of experimental data of RC (Reinforcement Concrete) wall. However, few data have been collected from recent research to evaluate the local behavior and damage of SC (Steel plate reinforced Concrete) wall, which is recently applied to the newly designed nuclear power plant. In this study, local damages of SC wall and RC wall against an idealized aircraft engine projectile impact are evaluated through FE simulation analyses with various wall thicknesses and steel ratio. Through analysis of local collision simulation results of SC and RC wall, the penetration depth of SC wall and RC wall are compared.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.20 no.3
• /
• pp.75-82
• /
• 2016

This study analytically reviewed the behavior of steel plate concrete (SC) walls subjected to forced vibration to investigate the effects of shape and arrangement spacing of studs on the behavior spacing of studs in SC wall were carried out. From the analyses, it was noted that the damping ratio obtained from the time history analyses showed overall high value in Half-power Bandwidth method and the lowest value in Fitted Exponential Curve method. And, in half of the design strength, the damping ratio presented approximately 3.0~4.2% and, in the design strength, it was approximately 4.1~5.2%. When the developed studs were used, the damping ratio was reduced slightly and it did not show consistent results between DS1 and DS2. When the distance between the studs increases more than necessary, it was also confirmed that the natural frequency was reduced and the damping ratio was increased.

• Archives of Pharmacal Research
• /
• v.18 no.6
• /
• pp.381-384
• /
• 1995

Covalent binding of the reactive metabolites of SC_42867 to microsomal proteins has been examined. In the absence of inhibitor of cytochrome oxydase (.alpha.-naphtyl-isothiocyanate) or a radical scavenger (3-terthiobuty-4-hydroxyanisol), up to 4.0% of total redioactivity used in the assay could irreversibly bind to proteins. In the presence of an inhibitor, the highest percentage of covalent binding observed is 0.7% a significant decrease of the metabolism of SC42876 was observed. These results suggest in a cytochrome P-450 dependent generation of SC_42867 metabolites significantly take part in the covalent binding process.

• Korean Journal of Poultry Science
• /
• v.44 no.3
• /
• pp.161-172
• /
• 2017

This study was conducted to determine the effect of Saccharomyces cerevisiae (SC) as a dietary probiotic and evaluated the most suitable feeding interval for this probiotic on growth performance, carcass yield, and meat quality parameters in broiler chickens. In total, 1,050 one-day-old Cobb 500 chicks were randomly assigned to one of seven dietary treatment groups, in a $2{\times}3$ factorial arrangement with 3 SC dosages (0.6%, 1%, and 1.4%) and two feeding intervals [long term (LT) for 35 days and short term (ST) for 28 days after hatching], with a negative control diet (NC; 0% SC). Triplicate experiments were performed with 50 birds per cage. Broilers fed a diet including SC showed increased (p<0.01) daily gain and feed efficiency compared to the control. Further, broilers fed the 1.4% SC supplemented diet showed a significantly increased (p<0.01) average daily gain (ADG) and feed conversion ratio (FCR) compared to broilers fed the 0.6% and 1% SC incorporated diets. Similarly, broilers fed an LT SC diet showed a greater (p<0.01) increase in ADG and FCR compared to broilers fed an ST SC diet. Moreover, broilers fed an LT SC diet displayed a reduced (p<0.05) meat pH, gizzard weight, and increased (p<0.05) meat water-holding capacity compared to broilers fed an ST SC diet. Broilers fed the 1.4% SC supplemented diet showed increased (p<0.05) thigh muscle weight compared to broilers fed the 0.6% and 1% SC supplemented diets. In conclusion, broilers fed LT SC diets showed improved growth performance and carcass quality parameters compared to broilers fed ST SC diets, and the NC diet, from hatching to day 35.

• Journal of the Korean Society for Heat Treatment
• /
• v.17 no.1
• /
• pp.23-28
• /
• 2004

In this work, the effect of Sc and Mm(misch metal) on the high temperature behavior of Al-Mg alloys was observed. Hardness was increased due to appearance of fine $Al_3Sc$ precipitates. The elongation of Al-Mg-Sc alloy at high temperature was higher than that of Al-Mg-Sc-Mm alloy because Al-Mg-Sc alloy has finer grain sizes than Al-Mg-Sc-Mm alloy. The strain rate sensitivity factor, "m" of Al-Mg-Sc and Al-Mg-Sc-Mm at $475^{\circ}C$ and $1{\times}10^{-2}s^{-1}$ were 0.33 and 0.46, respectively. The activation energy of Al-Mg-Sc and Al-Mg-Sc-Mm alloy for superplastic deformation was 133KJ/mol and 103KJ/mol respectively. The elongation of Al-Mg-Sc alloy at high temperature was decreased by the addition of Mm, but the strength at high temperatures and low strain rate was improved.

• Journal of Life Science
• /
• v.11 no.5
• /
• pp.457-463
• /
• 2001

A bacterial strain SC-22 which produced alkaline lipase was isolated from salf-fermented shrimps. Strains SC-22 was identified as Staphylococcus xylosus. An alkaline lipase excreted by Staphylococcus xylosus SC-22 was purified by ammonium sulfate predipitation and column chromatography on Sephadex G-100 and DEAE-Sephace. The specific activity of purified lipase was 756U/mg of protein with 17.2% yield. The approximate molecular weight of the purified enzyme was 47 kDa. The partially purified lipase preparation had and optimum temperature of 4$0^{\circ}C$, an optimum pH of 8.0, and a stable of 5~10. Lipase activities were enhanced by salt ions such as $Ca^{2+}$, $Mg^{2+}$,N $a^{2+}$ while inhibited remarkably by heavy metal ions, C $u^{2+}$ and P $b^{2+}$.EX> 2+/.