• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.50-55
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• 2014

Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.27 no.1
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• pp.35-40
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• 2021

Calcium hydroxide (CH) reacts with CO2 to produce moisture, and sodium carbonate (SC) reacts with CO2 in the presence of moisture. Using these different characteristics of these two reactants, a CH/SC mixture of CO2 absorber tuned for kimchi ripening to produce CO2 in a flexible package was selected. A ratio of CH:SC (1:2) in highly gas permeable microporous spunbonded film (Tyvek) sachet was found to be appropriate for delayed and consistent CO2 absorption useful for kimchi package. Addition of superabsorbent polymer (SAP) as moisture buffer was helpful for boosting the consistency of CO2 absorption. In a package of 0.5 kg kimchi at 10℃, the sachet consisting of 0.794 g of CH + 2.276 g of SC + 0.4 g of SAP suppressed its volume expansion and maintained a suitable range of CO2 partial pressure (PCO2) steadily inside. These optimal conditions may vary depending on the type and salinity of kimchi, storage and distribution temperature, and the material and area of the absorber sachet. This study showed a potential of mixture CO2 absorber to be tuned for CO2 producing packaged kimchi for the purpose of keeping consistent PCO2 at tolerable volume expansion.

• Korean Journal of Crystallography
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• v.10 no.1
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• pp.71-75
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• 1999

융액인상법에 의하여 Nd3+ 이온이 15 at% 주입된 LaSc3(BO3)4 단결정을 성장하였다. 최적의 결정성장 조건하에서 성장된 결정은 결정형이 잘 발달되고 보라색 투명하였다. 편광 현미경에 의한 결정결함 분석결과 성장된 결정 중심부에서 0.1 mm 크기의 기포가 검출되었고, B2O3가 증발하여 결정에 증착된 shoulder 부위에서는 균열이 발생되었으나 body에서는 결함이 검출되지 않았다. X선 회절에 의하여 cell parameter를 측정한 결과 a=7.73 , b=9.85 , c=12.05 , β=105.48o 및 공간군 C2/c의 monoclinic 구조로 분석되었다. 양질의 단결정을 성장하기 위한 결정성장 요소는 회전속도 10 rmp, 인상속도 1.5 mm/h이었다. 성장된 결정은 808 nm에서 강하고 넓은 흡수대와 1050∼1080 nm에 걸친 형광 방출대가 관찰되었다. 성장된 결정을 이용하여 직경 3 mm, 두께 1mm 크기의 micro-chip laser 소자의 제조기술을 확립하였다.

• Review of KIISC
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• v.11 no.6
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• pp.12-23
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• 2001

지식정보사회에서 모든 유통 및 관리 정보의 보호를 위하여 다양한 형태의 보호서비스가 활용되고 있다. 이러한 보호 서비스 중에서 민감한 정보의 비밀성을 보장하기 위한 암호 기법의 연구 개발과 이를 전자정부의 구현과 전자상거래와 같은 사업에의 적용에 따른 시장 점유와 연계된 표준화 활동이 암호기술 선진국을 중심으로 전개되고 있다. 이에 대하여 우리나라에서도 한국정보보호진흥원을 중심으로 국내 정보보호 분야 전문가들과 함께 개발하여 이미 TTAS.KO-12.0004(1999.9)로 단테 표준화한 SSED를 지난 ISO/IEC JTC 1/SC 27 동경회의(2000. 10)에 제안하여 최근 국내에서 산업자원부 기술표준원이 개최한 ISO/IEC JTC 1/SC 27 서울회의(2001. 10)를 통하여 3차 WD에 수용되기에 이르렀다. 본 고에서는 비밀성 서비스를 지원하는 블록 암호 알고리즘의 지역(AES, NESSIE, CRYPTREC) 및 국제(ISI/IEC JTC 1) 표준화활동을 분석 정리하고 특히 ISO/IEC JTC 1/SC 27에서 블록 암호 회의를 통한 SEED의 국제표준화 활동을 소개하기로 한다.

• Journal of Institute of Control, Robotics and Systems
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• v.7 no.1
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• pp.1218-1225
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• 2001

This paper presents a design procedure for the supervisory controller(SC) of the high-speed train car. The proposed SC manages control logic and scenario of the overall train system, and monitors input/output signals among the sub-controllers in the system, The roles and functions of each sub-control system are defined, and the interfaces among the control systems are developed. Train control system is modeled with automata, and the model is implemented into a program using a computer-aided software engineering(CASE) tool, Object GEODE, The designed SC is verified and evaluated by using simulation. The SC is shown to successfully perform the designed functions without any errors.

• Journal of Communications and Networks
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• v.17 no.1
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• pp.27-33
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• 2015

In this paper, we consider a joint frequency-domain scheduling and user-pairing problem for virtual MIMO in the single carrier frequency division multiple access (SC-FDMA) system, e.g., the uplink transmission for third generation partnership project-long term evolution (3GPP-LTE) standard. Due to the subcarrier adjacency constraint inherent to SC-FDMA, its complexity becomes unmanageable. We propose a greedy heuristic algorithm for PF scheduling so as to deal with the complexity issue in this joint problem. It has been shown that its performance can reach up to 90% of its upper bound.

• IMMUNE NETWORK
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• v.3 no.1
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• pp.23-28
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• 2003

Background: Intracellular antibody specific to hepatitis B virus X protein (HBx) might be useful for studying the role of HBx in hepatocellular carcinogenesis and HBV replication. Methods: With variable region genes for H7 monoclonal anti-HBx Ab, we constructed a vector for bacterial expression of single chain Ab (scFv) and a vector for eukaryotic cell expression of it. The expression of H7 scFv and its binding activity against HBx was examined by immunoblotting and immunofluorescence microscopy. Results: H7 scFv expressed in bacterial cells retained reactivity to HBx. We demonstrated its intracytoplasmic expression in CosM6 eukaryotic cells. Conclusion: This is the first study showing the expression of intracellular anti-HBx Ab in eukaryotic cells. H7 scFv may be a good tool to study the function of HBx in HBV infection.

• Journal of Plant Biotechnology
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• v.8 no.1
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• pp.9-14
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• 2006

As an alternative method to produce low cost reagents for immunodiagnosis and protect the plants from viral disease, a gene encoding a single chain variable fragment(scFv) recombinant antibody targeted to the coat protein of cucumber mosaic virus (CMV) was expressed in Nacotiana tabacum. The source of the scFv recombinant antibody gene was from spleen tissue of an immunized mouse. The gene was initially cloned into the pCANTAB5E phagemid and expressed in E. coli. In the following study, the antibody gene was subcloned into the plant expression vector, pCAMBIA-1301 and introduced into tobacco leaf tissue via Agrobacterium tumefacients mediated transformation. After transformation, 56 out of 58 plants were shown to carry the desired anti-CMV scFv gene by PCR analysis. Overall, only 12.5% of the 56 putative transgenic plants were found to express the antibody to a detectable level.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.4 no.1
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• pp.25-31
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• 1979

All the resitors in the active RC filter networks can be relplaced by the switched capacitors. Therefore, An SC filter circuit can be fully integrated using MOS technology. A switched capacitor is much better than a resistor in temperature and linearity characteristics, and the former can be fabricated on the much smaller area then the latter. In this paper, It is given the generalized disign method of the active SC filter from the active RC filter using Bilinear Z-transformation. By SC filtering Techniques using Bilinear Z-transform, It enalbes us to realize the FDNR and Gyrator filters, which could not be realized in the exsisting designs, and it permits the processing of signals at much higher frequenies that many previous designs do. Experiments show that the response of the SC active filter is similiar to that of its prototype active RC filter.

• Journal of information and communication convergence engineering
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• v.1 no.4
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• pp.217-222
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• 2003

In this paper, we introduce a hyper-chaos synchronization method using hyper-chaos circuit consist of State-Controlled Cellular Neural Network (SC-CNN). We make a hyper-chaos circuit using SC-CNN with the n-double scroll. A hyper-chaos circuit is created by applying identical n-double scroll or non-identical n-double scroll and Chua's oscillator with weak coupled method to each cell. Hyper-chaos synchronization was achieved using GS(Generalized Synchronization) method between the transmitter and receiver about each state variable in the SC-CNN.