• Title/Summary/Keyword: SAM P6

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Secretion Stimulation of Golden Vernish Sap from Dendropanax morbifera Lev. (황칠(黃漆)나무의 칠액(漆液) 분필(分泌) 촉진(促進))

  • Kim, Sea Hyun;Jung, Nam Chul;Na, Chun Soo;Kim, Sam Sik
    • Journal of Korean Society of Forest Science
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    • v.87 no.2
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    • pp.253-259
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    • 1998
  • The stimulated secretion of golden varnish sap by the infection of microorganisms on the bark of Dendropanax morbifera Lev. was observed in the natural habitats. Four candidate strains of such microorganisms(i.e., Sb1, Sb2, Sb3 and Sb4) were isolated from the bark of D. morbifera which was infected in natural. Of them, Sb3 and Sb4 strains showed stimulated secretion of golden vernish sap with the greatest stimulation by Sb4 strains. The yield of golden varnish sap from the bark of D. morbifera was boosted about 3.4~7.7 times by the inoculation of Sb4 strain in compassion with that collected from lacquer tree(Rhus verniciflua Stokes) by the traditional method. The mycelium color of Sb4 strain was grayish white. The optimum growth temperatures and pH were ranged from 30 to $35^{\circ}C$ and from pH 6.5 to 7.0.

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Cloning and Sequencing of a Gene Involved in the Biosynthesis of Exopolysaccharide in Zoogloea Ramigera 115SLR (Zoogloea Ramigera 115SLR로부터 다당류 생합성에 관여하는 유전자의 분리 및 염기서열 결정)

  • Sam-Pin Lee;Min Yoo
    • Biomedical Science Letters
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    • v.6 no.1
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    • pp.1-9
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    • 2000
  • To identity the genes responsible for the biosynthesis of exopolysaccharide, recombinant plasmids pUEX10 and pLEX10 were constructed from plasmid pLEX3 which was isolated from the recombinant cosmid library of Zoogloea ramigera 115. The complete nucleotide sequence of the 1.7 kb genomic DNA insert in plasmid pUEX10 was determined. Its analysis identified two open reading frames (ORF3 & ORF4) which could encode two proteins. The amino acid sequence derived from ORF3 showed the homology with gumC protein in Xanthomonas campestris as well as exoP protein in Rhizobium melizoti. The partial amino acid sequence of ORF4 showed the homology with polysaccharide export protein in Thermotoga maritima. Z. ramigera 115SLR and Z. ramigera 115SLR/pLEX10 showed the similar pattern for EPS production. Yield of exopolysaccharides produced by Z. ramigera 115SLR and Z. ramigera 115SLR/pLEX10 was 0.26% (w/v) and 0.16% (w/v), respectively.

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Comparison of OK-432 and Doxycycline Pleurodesis for Malignant Pleural Effusions Caused by Lung Cancer (폐암에 의한 악성 흉막삼출환자에서 OK-432와 Doxycycline 흉막유착술의 비교)

  • Chung, Jae-Ho;Park, Moo-Suk;Cheong, Jae-Hee;Kim, Young-Sam;Chang, Joon;Kim, Joo-Hang;Kwak, Seung-Min;Kim, Sung-Kyu;Kim, Se-Kyu
    • Tuberculosis and Respiratory Diseases
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    • v.52 no.6
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    • pp.590-596
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    • 2002
  • Background : Lung cancer is the leading cause of malignant pleural effusions, which is currently most commonly treated using pleurodesis via bedside thoracostomy. Several agents had been used for the treatment of pleural sclerosis, but with differing efficacies and associated side effects. Our purpose with this study was to compare the efficacy, side effects and disease free survival times of patients being treated with OK-432 and doxycycline sclerotherapy in lung cancer induced malignant pleureal effusions. Materials and Methods : 79 patients who underwent pleurodesis with OK-432 and doxycycline, between Jan.1994 and Aug. 2001, were retrospectively reviewed. Response 30 days following pleurodesis were determined from chest radiographs, with the disease free survival time being evaluated according to the response. Results : The success rates, 30 day followint pleurodesis, with OK-432 and doxycycline 83 and 87%, respectively (p=0.677). With regard to the side effects, fever was more common when OK-432 was used (59%, p=0.001), and pain was more common with doxycycline use (73%, p=0.008). There was no significant difference in disease free survival times between OK-432 (13.6 Months) and doxycycline (11.6 Months) (p=0.532). Conclusion : with the use of OK-432, for pleurodesis, was as effective as doxycycline, can be considered as an alternative treatment for malignant effusion in patients with lung cancer.

The Clinical Study on Yangdorak Change with Idiopathic Facial Paralysis Patients (특발성안면신경마비환자의 양도락 변화에 대한 임상적 고찰)

  • Kim, Kyung-Ok;Yang, Jae-Chul;Mun, Kyung-Suk;Lee, Kyung-Yun;Jang, Jo-Ung
    • Journal of Acupuncture Research
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    • v.22 no.6
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    • pp.201-209
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    • 2005
  • Objectives : We investicated correlation between Yangdorak and Meridian at Idiopathic facial nerve paralysis patients. Methods : For Yangdorak examination, We made a comparison between 21 patients who were diagnosticated to Idiopathic facial nerve paralysis and 22 persons who were not. Results : 1. The stress showed the highest frequency in premonitory cause at onset. 2. In comparing results on the Yangdorak general mean, they were not statistically significant between two group(p<0.05). 3. In comparing results on the Stomach(ST)-meridian Yangdorak mean, Idiopathic facial nerve paralysis group is stastically significant in comparison with non-facial paralysis group(p<0.05). 4. In comparing results on the Small Intestine(SI)-meridian Yangdorak mean, Idiopathic facial nerve paralysis group is not stastically significant in comparison with non-facial paralysis group(p<0.05). Conclusion : Yangdorak may be used for a method to objective clinical examination. But further studies are required for a use of practical indicator.

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Evaluation of Tracheal Deviation in Patients with Thyroid Cancer (갑상선 암 환장서 기관지 편이 정도를 평가하는 방법의 연구)

  • Yoo, Young-Sam
    • Korean Journal of Bronchoesophagology
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    • v.16 no.2
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    • pp.138-144
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    • 2010
  • Background: Tracheal deviations are encountered frequently in head and neck tumors especially in thyroid cancer. Dyspnea and stridor are symptom calling for surgery. The method of evaluation in tracheal deviation is not well established yet. This paper is aimed to suggest objective tools to evaluate tracheal deviation in relation to cervical vertebra. Material and Method: Ten cases of thyroid cancers were recruited retrospectively. Tracheal inner shadow and shape of cervical vertebra were reconstructed three dimensionally using 3D-doctor to compare position of trachea in relation to vertebral body. Extent of deviation was scored in relation to both lateral borders of vertebral body. Angles between trachea and spinous process were measured in axial CT and compared between study group and control group. Results: Deviation scores were statistically significant between study group (mean=1.1) and control group (mean=0) (p=0.0008). Deviation angles at maximal tumor size in study group (mean=160.3 Degrees) and deviation angles of control group (mean=177.1 Degrees) were statistically significant (p=0.0007). Angles at maximal deviation of three dimensional images ages in study group (mean=162.6 Degrees) and deviation angles of control group (mean=177.1 Degrees) were statistically significant (p=0.0089). Conclusion: Tracheal deviation can be evaluated using scoring of three dimensional images and measuring angle between trachea and vertebral spine.

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A Numerical Prediction of Pollutant Material Budget during the Flood and Dry Season in Gwangyang Bay (광양만의 홍수기 및 갈수기의 오염물질수지 예측)

  • Kim, Jin-Hyuk;Lee, In-Cheol;Yoon, Han-Sam
    • Journal of Ocean Engineering and Technology
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    • v.23 no.4
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    • pp.25-31
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    • 2009
  • To predict pollutants during the flood and dry season in Gwangyang Bay, the net-fluxes and pollutant material budgets of COD, T-N, and T-P were calculated in Gwangyang Bay using a 2-D hydrodynamic model. Calculating the net-flux for each area in Gwangyang Bay showed that the net-fluxes in regions IV, V, and VII were increasing, but those of regions II, III, and VI were decreasing. In budget calculations for COD, T-N, and T-P in Gwangyang Bay, it was estimated that during the dry season the COD is approximately 1.6 times higher than during the flood season. The T-N during the flood season is approximately 7 times higher than during the dry season. However, the material budget for T-P in Gwangyang Bay predicted that it is almost nonexistent. Moreover, the central part of Gwangyang Bay (Region IV) has the highest material budget of overall pollutants.

Diagnostic value of eosinopenia and neutrophil to lymphocyte ratio on early onset neonatal sepsis

  • Wilar, Rocky
    • Clinical and Experimental Pediatrics
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    • v.62 no.6
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    • pp.217-223
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    • 2019
  • Purpose: To determine the diagnostic value of eosinopenia and the neutrophil-to-lymphocyte ratio (NLR) in the diagnosis of early onset neonatal sepsis (EONS). Methods: This cross-sectional study was conducted in the Neonatology Ward of R.D. Kandou General Hospital Manado between July and October 2017. Samples were obtained from all neonates meeting the inclusion criteria for EONS. Data were encoded using logistic regression analysis, the point-biserial correlation coefficient, chi-square test, and receiver operating characteristic curve analysis, with a P value <0.05 considered significant. Results: Of 120 neonates who met the inclusion criteria, 73 (60.8%) were males and 47 (39.2%) were females. Ninety (75%) were included in the sepsis group and 30 (25%) in the nonsepsis group. The mean eosinophil count in EONS and non-EONS groups was $169.8{\pm}197.1cells/mm^3$ and $405.7{\pm}288.9cells/mm^3$, respectively, with statistically significant difference (P<0.001). The diagnostic value of eosinopenia in the EONS group (cutoff point: $140cells/mm^3$) showed 60.0% sensitivity and 90.0% specificity. The mean NLR in EONS and non-EONS groups was $2.82{\pm}2.29$ and $0.82{\pm}0.32$, respectively, with statistically significant difference (P<0.001). The diagnostic value of NLR in the EONS group (cutoff point, 1.24) showed 83.3% sensitivity and 93.3% specificity. Conclusion: Eosinopenia has high specificity as a diagnostic marker for EONS and an increased NLR has high sensitivity and specificity as a diagnostic marker for EONS.

Effect of Natural Foods on the Inhibition of N-Nitrosodimethylamine Formation (천연식물성분이 N-Nitrosodimethylamine 생성억제에 미치는 영향)

  • 이수정;신정혜;정미자;성낙주
    • Journal of Food Hygiene and Safety
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    • v.15 no.2
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    • pp.95-100
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    • 2000
  • The effect of natural foods, utilizing the extracts or juices of teas(Green tea; Camellia sinesis, Du'chung; Eucommia ulmoides Oliver), medicinal plants(Eu sung cho; Houttuynia cordata Thunb, Sam back cho; Saurus Chinensis, Baek hwa sa seal cho; Oldenladia diffusa Roxb.) seaweeds(Laver; Porphyra tenera, Sea mustard; Undaria pinnatifida, Sea staghorn; Condium fragile) and vegetables(Sweet pepper; Capsicum annuum var. angulosum, Kale; Brassia oleracea var.. acephala, Cucumber; Cucumis sativus, Onion; Allium cepa) and fruits(Tomato; Lycopericon esculentum, Maesil; Prunus mume, Plum; Prunus saticina and Grape; Vitis spp.)on the inhibition of N-Nitrosodimethylamine(NDMA) formation was investigated from the various conditions. The inhibition effect was observed in vitro using the reaction fluids of pH 1.2, 4.2 and 6.0. From the teas and medicinal plants, there was a positive response of NDMA formation; however, From the seaweed extracts, there was a negative response of the inhibition effect of NDMA formation, and as the pH of reaction fluids and the amount of materials increase, the inhibition of NDMA formation was strengthened. The inhibition ratios by the level of pH are as follows: under pH 1.2 vegetable juice were 57.6∼99.7% and fruits were 35.9∼99.7%; under pH 4.2 vegetable juice were 55.0∼97.5% and fruits were 21.3∼96.8%. All of the materials observed has been proved and shown the inhibition effect of NDMA formation.

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Effects of Soil Acidity and Organic Matter by Application of Organic Materials and Soil Mulching with Pine Needles for Soil Surface Management in Blueberry Eco-Friendly Farming

  • Ahn, In;Kim, Sam-Hyun;Maeng, Woon-Young;Lee, In-Eae;Chang, Ki-Woon;Lee, Jong-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.6
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    • pp.556-562
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    • 2013
  • The blueberry eco-friendly farming requires the soil condition of pH 4.2 ~ 5.2 and high in organic matters for stable growth. Most of soil types of blueberry-growing land in Korea, however, belongs to alkaline soils with low organic matter content. As a result, the eco-friendly blueberry growers use peat moss and sulfur powder heavily to improve the soil condition, but the guideline on the effective use of organic materials was not established yet. Therefore, this sturdy was performed to investigate the effect of increasing soil acidity and organic matters by using organic materials. Among 5 organic materials, the pH of soil was lowest in degradable sulfur + bentonite (pH 4.1) and followed by Peat moss+Chaff+Pine Needlesmixtures(pH 4.5), OrganicAcid +vinegar (pH 4.7), Sulfur powder (pH 4.8), Temperature response Elutioner (pH 5.2). The soil organic matter content were increased in the following order: Peat moss+Chaff+Pine Needlesmixtures (8.4%) > degradable sulfur + bentonite (7.8%) > Organic Acid + vinegar (7.2%) > Sulfur powder ${\fallingdotseq}$ Temperature response Elutioner (6.3%). Although different in the degree, all organic materials treated was recognized a good material for improving soil pH and organic matter content. The plant height and stem diameter of blueberry were no clear difference among 5 organic materials. Another study was carried out to investigate amending soils with organic matter by soil mulching with pine needles for soil surface management in blueberry organic cultivation. The effect of increasing the soil pH by pine needle mulching in blueberry eco-friendly farming was recognized in four test fields. Pine needle mulching for soil surface management in blueberry appeared several advantages, such as improving of soil pH and organic matter content. Therefore, pineneedle mulching in blueberry organic farming is considered as the most efficient means of mulching cultivation for amending soil pH, weed suppression and moisture conservation among mulching materials.

유기질비료의 시용이 수수$\times$수단그라스 교잡종의 생산성, 사료가치 및 가축사육능력에 미치는 영향

  • Park, Sang-Su;Park, Jun-Hyeok;Lee, Ju-Sam
    • Proceedings of the Korean Society of Organic Agriculture Conference
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    • 2009.12a
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    • pp.133-139
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    • 2009
  • 1) 공시유기질비료의 전질소는 유기발효돈분(2.68 %) > 계분부산물퇴비(1.97 %) > 유기발효계분, 돈분부산물퇴비(1.92 %) > 유기퇴적우분(1.12 %) > 유기 생 우분(1.04 %) 순이었다. 또한 유기물 함량은 유기 생 우분(81.73 %) > 유기퇴적우분(75.52 %) > 계분부산물(68.25 %) > 유기발효돈분(63.37 %) > 돈분부산물(45.27 %) > 유기발효계분(32.05 %) 순이었다. 2) 수수$\times$수단그라스 교잡종의 건물수량은 1차에서는 유기발효계분(11.60 ton/ha)이 가장 많았으나 계분부산물퇴비(10.13 ton/ha), 돈분부산물퇴비(9.07 ton/ha)와의 유의차는 없었고, 2차 역시 유기발효계분(8.00 ton/ha)이 가장 많았으나 계분부산물퇴비(6.27 ton/ha), 돈분부산물퇴비(6.13 ton/ha)와의 유의차는 없었다(p<0.05). 3) CP 수량은 1차에서는 유기발효계분(0.69 ton/ha)이 가장 많았으나 돈분부산물퇴비(0.56 ton/ha), 계분부산물퇴비(0.55 ton/ha), 유기 생 우분(0.55 ton/ha)과의 유의차는 없었고, 2차에서는 유의차가 없었다(p<0.05). 4) TDN 수량은 1차에서는 유기발효계분(6.51 ton/ha)이 가장 많았으나 계분부산물 퇴비(5.80 ton/ha), 돈분부산물퇴비(5.08 ton/ha)와의 유의차는 없었고, 2차 역시 유기발효계분(4.80 ton/ha)이 가장 많이 나왔으나, 계분부산물퇴비(3.70 ton/ha)와 유의차는 없었다(p<0.05). 5) 상대사료가치(RFV)는 1차에서는 계분부산물퇴비 시비구가 80.66으로 유의하게 높았으나, 2차에서는 유기퇴적우분을 제외한 5개 시비구의 유의차는 없었다(p<0.05). 6) K는 유기발효계분(0.11 head/ha/yr)이 가장 높았으나 계분부산물퇴비(0.10 head/ha/yr), 돈분부산물퇴비(0.09 head/ha/yr)와 유의차는 없었고, $K_{CP}$ 역시 유기발효계분(7.23 head/ha/yr)이 가장 높게 나왔으나 돈분부산물퇴비(6.41 head/ha/yr), 유기 생 우분(6.15 head/ha/yr), 계분부산물퇴비(5.97 head/ha/yr)와의 유의차는 없었다(p<0.05). 또한 KTDN은 유기발효계분(8.90 head/ha/yr)이 가장 높게 나왔으나 계분부산물퇴비(7.49 head/ha/yr)와 유의차는 없었다(p<0.05). 7) 이상의 결과로 보았을 때, 유기발효계분이 수단그라스의 생산성, 사료가치 및 가축사육능력에서 가장 우수할 것으로 판단된다.

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