• Title/Summary/Keyword: SAG2

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Effects of Phosphorus Starvation on Fatty Acid Production by Microalgae Cultivated from Wastewater Environment (인 결핍에 따른 하수배양 미세조류의 지방산 특성 분석 연구)

  • Woo, Sung-Geun;Park, Joon-Hong
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.4B
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    • pp.253-259
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    • 2012
  • Wastewater-adapted microalgae such as Chlorella vulgaris AG10032, Ankistrodesmus gracilis SAG278-2 and Scenedesmus quadricauda AG10308 are useful biological resources for recovering biofuel and other bio-based materials from wastewater because of their efficient removals of nitrogen and phosphorus from wastewater and their high fatty acid contents in biomass. Although the concentrations of phosphorus typically vary in wastewater environment, very little is known about the effect of phosphorus concentration, especially phosphorus starvation, on microalgal fatty acid synthesis. This is partially due to the lack of methodological establishment for algal fatty acid analysis. In this study, we compared the analysis performances of microalgal fatty acids by two different methods; one is a non-polar GC (gas chromatography) column based method, which is generally used for microbial fatty acids, and the other is a polar WAX-type GC column method, which is typically used for plant fatty acids. And then, we explored the effect of phosphorus concentration levels on fatty acid production in microalgae cultivated from wastewater. As results, the polar WAX-type column method has better ability to separate poly unsaturated fatty acids (PUFA) including $C_{18:3}$ (linolenic acid), and was found to be more applicable in analyzing fatty acids from wastewater-cultivated microalgae than the non-polar column method. The fatty acid characterization by the WAX-type column method revealed little effect of phosphorus starvation on the quantity and composition of fatty acids from wastewater-cultivated microalgae.

Molecular Ecological Characterization of Wastewater Bacterial Communities in Response to Algal Growth (조류성장에 따른 하수 박테리아 군집 변화에 관한 분자생태학적 연구)

  • Lee, Ju-Youn;Lee, Jang-Ho;Park, Joon-Hong
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.11
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    • pp.847-854
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    • 2011
  • To deal with issues from global climate changes, renewable bioenergy has become important. Algae have been regarded as a good resource for biorefinery and bioenergy, and also have potential capability to remove nutrient and non-decompositional pollutants for wastewater advanced treatment. Although algal-bacterial ecological interaction would be a crucially important factor in using algae for wastewater advanced treatment and resource recovery from wastewater, very little is known about ecological interaction between algae and bacteria in a real wastewater environment. In this study, under a real municipal wastewater condition, we characterized wastewater pollutant treatability and bacterial communities in response to growth of Ankistrodesmus gracilis SAG278-2, which can grow in wastewater and has a high lipid contents. The growth of algal population using the wastewater was inhibited by increase in wastewater bacteria while bacterial survival and cellular decay rate were not influenced by the algal growth. Removals of recalcitrant organic matters and total nitrogen were improved in the presence of algal growth. According to T-RFLP and statistical analysis, algal growth affected time-course changes in bacterial community structures. The following 16S rRNA gene amplicon, cloning results showed that the algal growth changes in bacterial community structure, and that bacterial populations belonging to Sediminibacterium, Sphingobacterium, Mucilaginibacter genera were identified as cooperative with the algal growth in the wastewater.

Calculations of Equilibrium Species and Solution Combustion Process for Spray Combustion Synthesis (SCS) (분사연소합성(SCS)을 위한 평형종 계산과 용액연소공정)

  • ;;;;Gary L. Messing
    • Journal of the Korean Ceramic Society
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    • v.38 no.6
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    • pp.545-550
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    • 2001
  • 본 연구에서는 분사연소합성(SCS)을 위한 기초단계로서 용액연소합성에 대한 거동을 살펴보고자 알루미나 합성을 모델로 하였으며 이를 위해 전구체에 대한 열분해거동, 그리고 각 온도에서의 평형종 분압 계산 및 합성과정을 조사하였다. 각각의 열중량 분석(TGA) 결과 산화제와 환원제(연료)의 열분해 이력이 서로 다르게 나타났으며, 열역학 응용 프로그램인 ChemSag에 의한 평형종 분압의 계산에서 연소속도를 저하시킬 수 있는 $CO_2$와 수증기 가스 분압이 상당부분 존재하였다. 산화제/환원제 혼합물의 열분석(DTA/TG) 결과 산화제와 환원제의 열분해 거동의 차이, 그리고 매우 작은 시료의 양으로 인해 263$^{\circ}C$에서 발열피크가 매우 작게 나타났다. 열분석 시료에 비해 발열 에너지를 높이기 위해 산화제와 환원제 혼합 전구체를 비이커에서 증기압을 조절하며 가열시켜 본 결과 27$0^{\circ}C$에서 $\alpha$-Al$_2$O$_3$생성물을 얻을 수 있었다. 따라서 분사연소합성 반응을 통해 세라믹 원료를 합성하기 위해서는 연소과정 중 열분해 거동과 평형종의 분압을 고려하여야 한다.

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Nominative/Accusative Adpositions in Negative Auxiliary Constructions

  • No, Yong-Kyoon
    • Language and Information
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    • v.8 no.2
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    • pp.73-91
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    • 2004
  • The nominative and accusative postpositions in Korean may intervene between the negative auxiliary verb ANH and its complement verb phrase. As Korean is an OV language, this means that 'verb + {nom, acc} + ANH' as well as the simpler concatenation 'verb + ANH' is possible. This fact, together with an overwhelming regularity of these postpositions' optionality in virtually all constructions, poses a problem for formal approaches to the syntax of the language. Working in a constraint-based grammatical framework shaped by such works as Sag and Wasow (1999) and Copestake (2002), we put forth type hierarchies for major_class, which represents verb inflection, and for pos, which has two immediate subtypes, i.e., htrp_pos and ord_pos. What we call the 'half transparency' of the case postpositions separates them from all the other lexical items in the language. The type htrp_pos is used to constrain one of the two newly proposed head_comp_rules, where a newly proposed feature HEAD2 of a phrase inherits its value from the HEAD feature of the head word. The COMPS list of the negative auxiliary ANH is seen as containing a single phrase whose HEAD is a kind of nominal clause and whose HEAD2 is something that is one of the three maximal types: acc, nom, and null.

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Active Metal Brazing Applied to Joining of ZrO2-Ti Alloy (ZrO2-Ti합금의 활성금속 브레이징)

  • Kee, Se-Ho;Park, Sang-Yoon;Jung, Jae-Pil;Kim, Won-Joong
    • Journal of Welding and Joining
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    • v.30 no.3
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    • pp.38-43
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    • 2012
  • In this study, active metal brazing methods for $ZrO_2$ and Ti alloy were discussed. To get a successful metal-ceramic bonding, various factors (melting temperature, corrosion, sag resistance, thermal expansion coefficient etc. of base materilas and filler metal) should be considered. Moreover, in order to clarify bonding between the metal and ceramic, the mechanism of the interfacial structure of the joints should be identified. The driving force for the formation of metal and ceramic interfaces is the reduction of the free energy which occurs when their contact becomes complete. Interfacial bonding depends on the material combinations and the bonding processes. This study describes the bonding between ceramic and metal in an active metal brazing.

A role of carboxy-terminal region of Toxoplasma gondii-heat shock protein 70 in enhancement of T. gondii infection in mice

  • Mun, Hye-Seong;Norose, Kazumi;Aosai, Fumie;Chen, Mei;Yano, Akihiko
    • Parasites, Hosts and Diseases
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    • v.38 no.2
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    • pp.107-110
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    • 2000
  • We investigated the role of recombinant Toxoplasma gondii heat shock protein (rT.g.HSP) 70-full length, rT.g. HSP70-NH2-terminal region, or rT.g. HSP70-carboxy-terminal region in prophylactic immunity in C57BL/6 mice perorally infected with Fukaya cysts of T. gondii. At 3, 4, 5, and 6 weeks after infection, the number of T gondii in the brain tissue of each mouse was measured by quantitative competitive-polymerase chain reaction (QC-PCR) targeting the surface antigen (SAG) 1 gene. Immunization with rT.g.HSP70-full length or rT.g.HSP70-carboxy-terminal region increased the number of T. gondii in the brain tissue after T. gondii infection, whereas immunization with rT.g.HSP70-NHa-terminal region did not. These results suggest that T.g. HSP70-carboxy-terminal region as well as T.g.HSP70-full length may induce deleterious effects on the protective immunity of mice infected with a cyst-forming T. gondii strain, Fukaya.

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Operation modes and Protection of VS(Vertical Stabilization) Converter for International Thermonuclear Experimental Reactor (국제 핵융합실험로용 VS(Vertical Stabilization) 컨버터의 운전모드 및 보호동작)

  • Jo, Hyunsik;Jo, Jongmin;Oh, Jong-Seok;Suh, Jae-Hak;Cha, Hanju
    • The Transactions of the Korean Institute of Power Electronics
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    • v.20 no.2
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    • pp.130-136
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    • 2015
  • This study describes the structure and operation modes of vertical stabilization (VS) converter for international thermonuclear experimental reactor (ITER) and proposes a protection method. ITER VS converter supplies voltage (${\pm}1000V$)/current (${\pm}22.5kA$) to superconducting magnets for plasma current vertical stabilization. A four-quadrant operation must be achieved without zero-current discontinuous section. The operation mode of the VS converter is separated in 12-pulse mode, 6-pulse mode and circulation current mode according to the magnitude of the load current. Protection measures, such as bypass and discharge, are proposed for abnormal conditions, such as over current, over voltage, short circuit, and voltage sag. VS converter output voltage is controlled to satisfy voltage response time within 20 msec. Bypass operation is completed within 60 msec and discharge operation is performed successfully. The feasibility of the proposed control algorithm and protection measure is verified by assembling a real controller and implementing a power system including the VS converter in RTDS for a hardware-in-loop (HIL) facility.

Fast Envelope Estimation Technique for Monitoring Voltage Fluctuations

  • Marei, Mostafa I.;Shatshat, Ramadan El
    • Journal of Electrical Engineering and Technology
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    • v.2 no.4
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    • pp.445-451
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    • 2007
  • Voltage quality problems such as voltage sag, swell, flicker, undervoltage, and overvoltage have been of great concern for both utilities and customers over the last decade. In this paper, a new approach based on the $H_{\infty}$ algorithm to monitor voltage disturbances is presented. The key idea of this approach is to estimate the amplitude of the fundamental component of distorted and noisy voltage waveform instantaneously, and then the information can be extracted from the estimated envelope to identify and classify different voltage related power quality problems. The $H_{\infty}$ algorithm is characterized by a fast tracking, unlike that of existing techniques. The $H_{\infty}$ algorithm outperforms the Kalman Filter (KF) by its fast convergence and robust tracking performance against non-Gaussian noise. The paper investigates the effects of various types of noise on the performance of the $H_{\infty}$ algorithm. Digital simulation results confirm the validity and accuracy of the proposed method. The proposed $H_{\infty}$ algorithm is examined by tracking the flicker produced by a resistance welder simulated in the PSCAD/EMTDC package.

Current Limiting Characteristics of a SFCL with Two Triggered Current Limiting Levels in a Simulated Power Distribution System (모의배전계통에 두 트리거 전류레벨을 이용한 초전도한류기의 전류제한 특성 분석)

  • Ko, Seok-Cheol;Han, Tae-Hee
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.26 no.2
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    • pp.134-139
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    • 2013
  • When the accident occurred in power distribution system, it needs to control efficiently the fault current according to the fault angle and location. The flux-lock type superconducting fault current limiters (SFCL) can quickly limit when the short circuit accidents occurred and be made the resistance after the fault current. The flux-lock type SFCL has a single triggering element, detects and limits the fault current at the same time regardless of the size of the fault current. However, it has a disadvantage that broken the superconductor element. If the flux-lock type SFCL has separated structure of the triggering element and the limiting element, when large fault current occurs, it can reduce the burden of power and control fault current to adjust impedance. In this paper, this system is composed by triggering element and limiting element to analyze operation of limiting current. When the fault current occurs, we analyzed the limiting and operating current characteristics of the two triggering current level, and the compensation characteristics of bus-voltage sag according to the fault angle and location.

Antisense expression of a staygreen gene (SGR) delays leaf senescence in creeping bentgrass

  • Hwang, Ok-Jin;Han, Yun-Jeong;Paek, Nam-Chon;Kim, Jeong-Il
    • Rapid Communication in Photoscience
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    • v.3 no.2
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    • pp.28-31
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    • 2014
  • Loss of chlorophyll is the visible symptom of leaf senescence and staygreen refers to the delayed leaf senescence in plants. The staygreen gene (SGR) in rice (Oryza sativa L.) has been identified as its mutation maintains greenness during leaf senescence, and encodes a chloroplast protein required for the initiation of chlorophyll breakdown in plants. In this study, we isolated a rice SGR-homologous gene in creeping bentgrass (Agrostis stolonifera L.), and transgenic creeping bentgrass plants were obtained by introducing pCAMBIA3301 vector harboring antisense SGR gene under control of the senescence-specific SAG12 promoter. Transgenic plants were selected by herbicide resistance assays and genomic integration of the transgenes was confirmed by PCR analysis. Subsequent analyses demonstrated the staygreen phenotype of the transgenic creeping bentgrass plants with decreased chlorophyll loss during leaf senescence. These results suggest that the antisense SGR expression in creeping bentgrass delays leaf senescence, which provides a way to develop genetically engineered turfgrass varieties with the commercially useful staygreen trait.