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Selection of Culture Scale for Stable Culture of an Estunrine Cladoceran Diaphanosoma celebensis (기수산 물벼룩 Diaphanosoma celebensis의 안정 배양을 위한 배양 용기의 크기 선택)

  • JUNG Min-Min;KIM Hyeung-Sin;RHO Sum
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.4
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    • pp.466-469
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    • 1999
  • Cladocera are important food organism for seed production of finfishes. freshwater cladocera such as Daphnia and Moina are well known food organisms for the larval rearing of freshwater fishes and are easy for mass culture. However, mass culture technique for marine cladocera are not yet developed, The only mass produced food organisms available these days for the larval production of marine finfishes are rotifer and Artemia. An estuarine cladoceran, Diaphanosoma celebensis, has a high possibility of being used as a food organism for the larval rearing of marine finfishes because this species is much easier to mass culture than marine ones. Therefore many studies are needed for this species. In this study, the effects of the volumes of culture container, 40, 1,500 and 15,000 ml, on the stable production of this species were tested and results are as follow: The maximum densities of this species in each of the culture volumes were reached after 14 days in 40 ml, 12 days in 1,500 ml, and 21 days in 15,000 ml with values of 3.4 $\pm$ 0.4, 14.2 $\pm$ 2.1 and 2.5 $\pm$ 1.6 per ml, respectively. The relative population growth index (RPGI) was stable in the culture volume of 1,500 ml. Moreover, possible harvesting number(individual/ml/day) was much higher in the 1,500 ml container than the other culture volumes. Therefore, optimum culture volume among the tested volumes for mass production of this species was 1,500 ml.

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Anti-inflammatory and Antioxidant Activities of Ulva lactuca Linnaeus Extract (참갈파래 추출물의 항염증 및 항산화 활성)

  • Min, Kyung-Cheol;Kim, Geun-Dae;Nam, Hyeon-Gyu;Lee, Sang-Hyeon
    • Journal of Life Science
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    • v.32 no.5
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    • pp.340-347
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    • 2022
  • The anti-inflammatory and antioxidant activities of the Ulva lactuca Linnaeus extracts were measured. As a result of the MTT assay of the extract, it was found that the cell viabilities were higher than 90% at all concentrations, and the cell promotion rate exceeded 100% at a certain concentration. Therefore, in all subsequent experiments, the highest concentration was set at 500 ㎍/ml. In the nitric oxide (NO) assay, one of the anti-inflammatory experiments, the Ulva lactuca Linnaeus extracts exhibited inhibitory effects of 16%, 19%, and 62% at concentrations of 5, 50, and 500 ㎍/ml, respectively, compared with the LPS-only group. In tumor necrosis factor-α (TNF-α) assay, the extracts showed inhibitory effects of 16%, 17%, and 27% at concentrations of 5, 50, and 500 ㎍/ml, respectively, compared with the LPS-only group. In the interleukin-6 (IL-6) assay, the extracts also showed inhibitory effects of 15% and 28% at concentrations of 50 and 500 ㎍/ml, respectively, compared with the LPS-only group. In the total polyphenol content assay, the extracts exhibited 8.02, 9.90, and 23.8 mg GAE/g at concentrations of 0.3, 3, and 30 mg/ml, respectively. In the ABTS assay, the extracts exhibited scavenging activities of 2.6, 12.6, and 77.3% at concentrations of 0.3, 3, and 30 mg/ml, respectively. Therefore, it is considered that Ulva lactuca Linnaeus extracts can be used as anti-inflammatory and antioxidant substances for the development of functional foods.

Inactivation of Escherichia coli O157:H7, Salmonella and Listeria monocytogenes by Organic Acid (유기산이 Escherichia coli O157:H7, Salmonella 및 Listeria monocytogenes의 증식에 미치는 영향)

  • Jang, Jae-Seon;Lee, Hye-Jeong;Oh, Bo-Young;Lee, Jea-Mann;Go, Jong-Myeong;Kim, Yong-Hee
    • Journal of Environmental Health Sciences
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    • v.33 no.5
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    • pp.403-407
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    • 2007
  • The inhibitory effect of the food processing agent on growth of Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes was performed with organic acid, and combination of citric acid, acetic acid, propionic acid and vanillic acid. The minimun inhibitory concentration(MIC) of propionic acid was 5,000 ppm in E. coli O157:H7, 2,500 ppm in Salmonella Enteritidis and Listeria monocytogenes. MIC of citric acid was 10,000 ppm in E. coli O157:H7 and Salmonella Enteritidis, 2,500 ppm in Listeria monocytogenes. MIC of acetic acid was 2,500ppm, while in vanillic acid was 5,000 ppm in Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes. MIC of combined organc acid in E. coli O157:H7 were 2,500ppm in PC, 1,250 ppm in PA, PV, CA, CV and AV. MIC of combined organc acid in Salmonella Enteritidis were 2,500 ppm in PC, PA, PV, CA, and CV, 1,250 ppm in AV. MIC of combined organc acid in Listeria monocytogenes were 1,250 ppm in all treatment group. MIC of combined treatment of three organc acid in E. coli O157:H7, S. Enteritidis and L. monocytogenes were 1,250 ppm in PCA, PCV, PAV and CAV. The inhibitory effect of organc acid in E. coli O157:H7, S. Enteritidis and L. monocytogenes could be confirmed from the result of this experiment. Therefore, it was expected that the food process would increase or maintain by using organic acid.

Effect of a polyherbal formulation on anxiety and behaviour mediated via monoamine neurotransmitters

  • Balaraman, R;Mohan, M;Aurangabadkar, VM;Jadhav, GB;Austin, Anoop;Thirugnanasampathan, Thirugnanasampathan
    • Advances in Traditional Medicine
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    • v.7 no.4
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    • pp.409-417
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    • 2007
  • We investigated the effect of Arogh, a polyherbal formulation (PHF) on animal models of anxiety based on exploratory behavior. The anxiolytic activity of polyherbal formulation (30, 100, 300 and 500 mg/kg) was studied using various behavioural paradigms such as elevated plus maze (EPM), light/dark apparatus (LDA), open field apparatus (OFA), hole board apparatus (HBA). Diazepam (1 mg/kg) was used as a standard anxiolytic drug. The effect of PHF (100 and 300 mg/kg) on serotonin, dopamine and noradrenaline mediated behaviour was studied by lithium induced head twitches in rats, haloperidol induced catalepsy in mice and clonidine induced hypothermia in rats respectively. In EPM, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the time spent in open arms and the number of entries in open arms. In LDA, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the time spent in lit zone. In OFA, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the number of assisted rearing and the number of squares traversed. In HBA, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the number of head poking. In lithium induced head twitches, PHF (100 and 300 mg/kg) significantly (P < 0.05) decreased the number of head twitches. In haloperidol induced catalepsy, PHF (300 mg/kg) decreased the duration of catalepsy significantly (P < 0.05) at 60 min. In clonidine-induced hypothermia, PHF (300 mg/kg) did not modify the effect. Drugs must be carefully assessed on EPM test and therefore in the present study EPM is supported by other tests. Present study indicates that Arogh, a polyherbal formulation possess anxiolytic activity. It diminished serotonergic transmission and decreased the duration of catalepsy indicating potentiation of dopaminergic transmission. Thus, Arogh a polyherbal formulation contains bioactive principles which possess anxiolytic activity and modified 5-HT and DA mediated behaviour.

Antinociceptive and gastro-protective effect of the ethanolic extract of the flowering top of Anthocephalus Cadamba Roxb

  • Subhan, Nusrat;Hasan, Raquibul;Hossain, Mokarram;Akter, Raushanara;Majumder, Muntasir Mamun;Rahman, Mostafizur;Ahmed, Kamaluddin;Ghani, Abdul;Alam, Ashraful
    • Advances in Traditional Medicine
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    • v.9 no.4
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    • pp.326-334
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    • 2009
  • The effect of alcoholic extract of Anthocephalus (A.) Cadamba Roxb. was evaluated in experimental models of pain and ulcer. Hot tail flick test, hot plate test and acetic acid induced writhing test were employed for evaluating the peripheral as well as central analgesic mechanism exerted by the extracts. Gastroprotective activity was examined by HCl and ethanol induced gastric damage test. Test group received crude extract 500 mg/kg showed maximum time needed for the response against thermal stimuli (6.26 ${\pm}$ 0.439 s) which is comparable to diclofenac sodium (6.56 ${\pm}$ 0.381 s) in hot tail flick method. These experimental results also followed the experimental results of hot plate test where crude extract 500 mg/kg showed maximum time needed for the response against thermal stimuli (4.74 ${\pm}$ 0.234 s) which is comparable to diclofenac sodium (5.58 ${\pm}$ 0.585 s). The crude extract at 500 and 250 mg/kg showed significant reduction in acetic acid induced writhing in mice with a maximum effect of 68.026% reduction at 500 mg/kg dose which is comparable to standard diclofenac sodium (79.93%). In gastroprotective study the extract of A. Cadamba (250 and 500 mg/kg) significantly inhibited ulceration induced by both HCl and ethanol dose dependently. Results of the study suggest that the extract possesses both analgesic and gastroprotective activity on mice.

A study on wideband strip-line balun using a power divider and a phase converter (전력 분배기와 위상 변환기를 이용한 광대역 스트립라인 발룬에 관한 연구)

  • Lee, Chang-Seok;Park, Ung-Hee
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.12 no.7
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    • pp.1160-1164
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    • 2008
  • This paper presents an wideband stripline balun. And, the proposed balun is composed of one power divider and two phase converters on stripline substrate. The target characteristics of the proposed balun is the combination loss of below -0.05 dB, an amplitude imbalance of ${\pm}0.5\;dB$ and a phase imbalance of $180{\pm}10$ degrees, with the reflection coefficient(S11) of below -10 dB over frequencies ranging from 500 to 1500 MHz. The fabricated balun occupies the area of $12(W){\times}220(L){\times}2.3(T)\;mm3$. Experimental measurement shows that the fabricated balun has an amplitude imbalance of ${\pm}0.7\;dB$, a phase imbalance of $180{\pm}8$ degrees and an insertion loss of about -2 dB with the reflection coefficient(S11) of below -10 dB over frequencies ranging from 500 to 1,500 MHz.

EFFECT OF RECOMBINANT BOVINE SOMATOTROPIN ON GROWTH PERFORMANCE AND CARCASS CHARACTERISTICS OF FINISHING KOREAN NATIVE BULLS

  • Kwak, B.O.;Ha, J.K.;Chang, B.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.1
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    • pp.101-106
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    • 1996
  • This experiment was conducted to investigate the effect of bST administration on growth performance and carcass characteristics of finishing Korean native bulls. Fifty four Korean native bulls of about 14 months of age weighting an average 420 kg were assigned to receive no injection of bST, 250 mg of bST s.c. injection every week, or 500 mg bST s.c. injection every 2 weeks (Control, 250ST, 500ST) in a $3{\times}3$ randomized block design for a period of 20 weeks Animals administered with bST responded with decreased feed DMI by 8%(p < 0.01), increased ADG by 12%(p < 0.05), and increased gain/feed ratio by 20%(p < 0.01). But there was no significant difference in the growth performance between 250ST and 500ST. Administration of bST increased dressing percentage by 1.8%(p < 0.05), and tended to increase longissimus muscle area and decrease backfat thickness and marbling score.

The Effect of Mineral Salts, Magnesium Sulphate and Potassium Nitrate on the Economic Parameters of Silkworm Bombyx mori L.

  • Goudar, K.S.;Kaliwal, B.B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.3 no.1
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    • pp.69-73
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    • 2001
  • The effect of topical application 100,300 and 500 $\mu$g/ ml with Mineral Salts of magnesium sulphate and potassium nitrate on economic parameters was ana- lyzed following treatment of last larval stadium. The treated larvae showed significantly decreased larval weight and silk gland weight in all the treated groups along with other decreased Iarval, cocoon and adult parameters. The female cocoon weight was significantly decreased in all the treated groups with decreased female cocoon shell weight and male cocoon weight at 300 and 500$\mu$g/ml respectively. The length and weight of filament was significantly decreased at 300 and 500 $\mu$g/ml respectively and denier at 500 $\mu$g/ ml. The fecundity decreased significantly in 300 and 500 $\mu$g/ml treated groups when compared with the corresponding parameters of the carrier control.

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The Analysis Results of Lightning Overvoltages by EMTP for Lightning Protection Design of 500kV Substations

  • Ju Hyung-Jun;Lee Heung-Ho
    • KIEE International Transactions on Power Engineering
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    • v.5A no.4
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    • pp.366-370
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    • 2005
  • To meet increasing power demand, 500 kV power systems are under consideration in the regions of some Middle Asian countries. As the power system voltage becomes higher, the cost for the power system insulation increases significantly. 500 kV transmission systems will become the basis of a region's power system and they require much higher system reliability. Consequently, by the methods of limiting overvoltages effectively, a reasonable insulation design and coordination must be accomplished. In particular, the Substations must be constructed to be of outdoor type. In order to determine the various factors for the insulation design, the EMTP (Electro-magnetic transient program) is used for the magnification of transient phenomena of the 500 kV systems in the planned network. In this paper, we will explain the calculation results of lightning overvoltages by the EMTP for lightning protection design for the 500 kV substations. To obtain reliable results, the multi-story tower model and EMTP/TACS model are introduced for the simulation of dynamic arc characteristics.

Japan Vending Industry News

  • 한국자동판매기공업협회
    • Vending industry
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    • v.5 no.1 s.13
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    • pp.84-88
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    • 2005
  • 일본 자판기 시장은 최근 위조 500엔 동전 문제가 핫이슈로 대두되고 있다. 과거 한국의 500원 동전을 이용한 자판기 털기 분제가 엄청난 파장으로 확산되어 신500엔 동전 발행으로 이어졌던 게 사실. 500엔 문제는 그 후 잠잠해지는 가 했더니 최근 새로운 위변조 동전이 다량 출몰하여 다시 사회문제화가 되고 있다. 일반 유인 유통은 물론 자판기, ATM등에 막대한 파급력이 예상되고 있기 때문에 일본 정부나 유관단체 등에서는 본격적인 대책강구에 분주해 지고 있다. 또한 500엔 동전 이외에도 1000엔권, 10000엔권 등에서도 위조지폐가 증가하고 있는 추세에 있는 등 일본사회는 ‘위폐와의 전쟁’을 앞으로 불사해야 할 상황이다. 금호 일본뉴스에서는 위폐문제로 골머리를 앓고 있는 현실들을 자세하게 담아봤다. 이외에도 점점 입지가 좁아지는 담배자판기 문제, 수난을 당하는 유해 도서 자판기 등의 뉴수도 다채롭게 준비했다. 일번 일본 뉴스들은 삶에 있어 자판기의 비중이 증대되면 될 수록 사회적인 역할과 책임이 커져야 한다는 것을 시사하고 있다.

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