• Title/Summary/Keyword: S-locus

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Isolation of the Regulator Gene Responsible for Overproduction of Catalase A in $H_2O$$_2$-resistant Mutant of Streptomyces coelicolor

  • Hahn, Ji-Sook;Oh, So-Young;Keith F. Chater;Roe, Jung-Hye
    • Journal of Microbiology
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    • v.38 no.1
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    • pp.18-23
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    • 2000
  • Streptomyces coelicolor produces three kinds of catalases to cope with oxidative stress and to allow normal differentiation. Catalase A is the major vegetative catalase which functions in removing hydrogen peroxide generated during the process of aerobic metabolism. To understand the regulatory mechanism of response against oxidative stress, hydrogen peroxide-resistant mutant (HR4O) was isolated from S. coelicolor J1501 following UV mutagenesis. The mutant overproduced catalase A more than 50-fo1d compared with the wild type. The mutation locus catRI was mapped closed to the mthB2 locus by genetic crossings. An ordered cosmid library of S. coelicolor encompassing the mthB2 locus was used to isolate the regulator gene (catR) which represses catalase overproduction when introduced into HR4O. A candidate catR gene was found to encode a Fur-like protein of 138 amino acids (15319 Da).

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Genetic Responses to Metal ion in Aslmonella typhimurium (Salmonella typhimurium의 금속이온에 대한 유전적 반응)

  • Jung, Ju-Ri;Park, Kyeong-Ryang;Koh, Sang-Kyun;Park, Yong-Keun;Lee, In-Soo
    • Journal of Life Science
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    • v.8 no.2
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    • pp.216-225
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    • 1998
  • Metal ion-induced and it’s regulatory genes were screened in virulent salmonella typhimurium UK1 and tested cross-regulation with various stresses. Using the techniqud of P22-MudJ(Km, lacZ)-directed lacZ operon fusion, LF40 cuiA::MudJ and Lf153 cuiD::MudJ which were induced by copper were selected. cuia and cuiD were determined anaerobic coper inducible and copper tolerance response gene, respectively. Also cuiA and cuiD locus were determined at 81 and 8min, respectively, on salmonella Genetic Map. The two regulators were identified as cuaR, and cudR, which controls cuiA and cuiD, respectively. cuaR, and cudR appeared as negative regulators because the expression of cuiA-lac-Z and cuiD-lacZ were increased. Copper adapted UK1 showed high resistance to H$_{2}$O$_{2}$, but cuiD did not. The product of the cudR locus was responsible for decreasing the tolerance to copper and H$_{2}$O$_{2}$. Furthemore cuiA and cuiD locus were found to be part of a regulon under the control of a trans-acting regulators, rpoS, oxyR and relA. Therefore, the results suggest CTR participate with oxidative stress on Salmonella.

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Genetic Diversity of Korean Cyanobacteria determined by DNA polymorphisms within the Phycocyanin Locus (Phycocyanin locus내의 DNA Polymorphism에 의한 한국산 Cyanobacteria의 유전적 다양성)

  • 박진숙;권주리;유순애
    • Korean Journal of Microbiology
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    • v.36 no.4
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    • pp.249-253
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    • 2000
  • The genetic diversity among Korean cyanobacteria was assessed by restriction fragment length polymorphism(RFLP) analysis of PCR products from the phycocyanin locus. Strains of all the genera tested were successfully amplified, and the size of amplified fragments was approximately 700bp. The restriction patterns generated by AluI, MspI, and HaeIII were conserved for strains within each of genera studied and were specific to the genus level. Intrageneric delineation of strains was revealed by the enzyme, CfoI for members of genera Anabeana and Synechocystis. Phenogram derived from the different RFLP patterns revealed a coherent cluster among Anabeana, Chlorogloea, and Synechocystis strains. PC-RFLP methods provided useful tools for classification of cyanobacteria.

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Exploration of genetic diversity of Bacillus spp. from industrial shrimp ponds in Vietnam by multi-locus sequence typing

  • Le, Xuan The;Pham, Dung Tien;Pham, Tuan Anh;Tran, Tung Thanh;Khuat, Thanh Huu;Le, Hoa Quang;Vu, Ut Ngoc
    • Fisheries and Aquatic Sciences
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    • v.22 no.8
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    • pp.17.1-17.9
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    • 2019
  • Bacillus is a diverse genus consisting of more than 200 species with extensive genetic diversity. Their beneficial effects in industrial shrimp farming have been well documented. However, little is known about the biodiversity of the Bacillus spp. in this aquaculture system. Taxonomic analysis by 16S rRNA sequencing does not always allow species-level identification of Bacillus spp. In this study, 26 Bacillus isolates from two industrial Litopenaeus vannamei shrimp ponds in Bac Lieu Province, Vietnam, were analyzed for their genetic diversity by multi-locus sequence typing (MLST). A total of 22 sequence types were identified and segregated into four distinct clusters, corresponding to B. subtilis, B. velezensis, B. siamensis, and B. licheniformis. Bacillus subtilis and B. velezensis accounted for more than 73% of the Bacillus isolates. Notably, the MLST scheme exhibited high discriminatory power and might be further simplified to be a convenient method to identify species of the genus Bacillus.

Development of a Sequence Characteristic Amplified Region Marker linked to the L4 Locus Conferring Broad Spectrum Resistance to Tobamoviruses in Pepper Plants

  • Kim, Hyun Jung;Han, Jung-Heon;Yoo, Jae Hyoung;Cho, Hwa Jin;Kim, Byung-Dong
    • Molecules and Cells
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    • v.25 no.2
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    • pp.205-210
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    • 2008
  • To develop molecular markers linked to the $L^4$ locus conferring resistance to tobamovirus pathotypes in pepper plants, we performed AFLP with 512 primer combinations for susceptible (S pool) and resistant (R pool) DNA bulks against pathotype 1.2 of pepper mild mottle virus. Each bulk was made by pooling the DNA of five homozygous individuals from a T10 population, which was a near-isogenic $BC_4F_2$ generation for the $L^4$ locus. A total of 19 primer pairs produced scorable bands in the R pool. Further screening with these primer pairs was done on DNA bulks from T102, a $BC_{10}F_2$ derived from T10 by back crossing. Three AFLP markers were finally selected and designated L4-a, L4-b and L4-c. L4-a and L4-c each underwent one recombination event, whereas no recombination for L4-b was seen in 20 individuals of each DNA bulk. Linkage analysis of these markers in 112 $F_2$ T102 individuals showed that they were each within 2.5 cM of the $L^4$ locus. L4-b was successfully converted into a simple 340-bp SCAR marker, designated L4SC340, which mapped 1.8 cM from the $L^4$ locus in T102 and 0.9 cM in another $BC_{10}F_2$ population, T101. We believe that this newly characterized marker will improve selection of tobamovirus resistance in pepper plants by reducing breeding cost and time.

A Study on the Relationship Between Health Protective Behavior and Health Locus of Control (예방적 건강행위와 건강통제위 성격 성향과의 관계에 대한 연구)

  • 김정희
    • Journal of Korean Academy of Nursing
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    • v.15 no.2
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    • pp.49-61
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    • 1985
  • It is assumed that the more society advances in its complexity and development, the more people pay attention to their health and accordingly the more people tend to practice health protective behavior. Most of human behavior is based on social inter-actions. The concept in Locus of Control has been developed from social learning theory to help better understanding the social phenomena affecting human behavior. Multidimensional Health Locus of Control is consisted of three dimensions: Internal, Powerful Others and Chance. This study was conducted to find out the health protective behavior patterns of the people and its relationship with Health Locus of Control as an influencing factor to their behavior. All the subjects in this study were the ones of the bank employees in Seoul, Among the total of 1,430 bank employees, 761 were chosen as a sampled subjects. Questionnair survey was conducted from March 4 to March 13, 1985. Research instruments used in this study were two kinds, one was the Health Locus of Control Scale developed by Wallston & Wallston and the other was the Measuring Scale for Health Protective Behavior developed by the investigator. Analysis of data was done by using Descriptive Statistics, X²-test, t-test, ANOVA, Pearson's correlation coefficient. The results of this study are summarized as follows: The mean score of Internal was 24.1, Powerful others was 19.7 and Chance was 15.3 out of maximum range of 6-30 respectively. The mean score of Health Protective Behavior Scale fell as 53.2 out of a maximum range of 18-90. Internals were more likely closely related with sex, educational levels and religion, Powerful others were related with age, educational levels and the number of family members. Chances were related with edu cational levels. The older the subjects were, the more concerned about their health. They tended to practice more favorable health protective behaviors. Furthermore, married people tended to fay more attention to their health than single individuals. Also, the number of family members and religion affected their attitudes in the health protective behavior patterns. Internals and Powerful Others were related with health protective behaviors. If one believes he can do something about his health and others play a significant role on his health, he is more apt protective patterns more suitable for health and actually shows that he is better off.

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Exploring What Effects on Vaccination for Covid-19: Converging Health Locus of Control and Health Belief Model (코로나 19 백신 접종영향 요인의 탐색: 건강통제소재와 건강신념모형의 융합)

  • Joo, Jihyuk
    • Journal of the Korea Convergence Society
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    • v.12 no.11
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    • pp.347-357
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    • 2021
  • Since the outbreak of Covid-19, many countries have tried to defense Covid-19 to protect their people and as an influential and reliable policy as of now, they have recommended vaccinating. Thus, this research explored what influences the intention to vaccinate against Covid-19 with three health locus of control from multi-dimension health locus of control (MHLC) and perceived susceptibility and severity from health belief model (HBM) through PLS path modeling. Consequently, chance locus of control (CHLC) influence indirectly intention to vaccinate against Covid-19 mediating with susceptibility perception. It implies that the more fatalistic people attitude toward Covid-19, the more susceptible they perceived to the disease, and then, the stronger intention to vaccinate they would have. Thus, the health promotion authorities should motivate to activate people's susceptibility perception toward the disease through utilizing a variety of policies and consider that the fatalistic tendency toward the disease of people could play an antecedent role in the process.

A Model for Health Promoting Behaviors in Late-middle Aged Woman (중년후기 여성의 건강증진행위 모형구축)

  • Park, Chai-Soon
    • Women's Health Nursing
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    • v.2 no.2
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    • pp.298-331
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    • 1996
  • Recent improvements in living standard and development in medical care led to an increased interest in life expectancy and personal health, and also led to a more demand for higher quality of life. Thus, the problem of women's health draw a fresh interest nowadays. Since late-middle aged women experience various physical and socio-psychological changes and tend to have chronic illnesses, these women have to take initiatives for their health control by realizing their own responsibility. The basic elements for a healthy life of these women are understanding of their physical and psychological changes and acceptance of these changes. Health promoting behaviors of an individual or a group are actions toward increasing the level of well-being and self-actualization, and are affected by various variables. In Pender's health promoting model, variables are categorized into cognitive factors(individual perceptions), modifying factors, and variables affecting the likelihood for actions, and the model assumes the health promoting behaviors are affected by cognitive factors which are again affected by demographic factors. Since Pender's model was proposed based on a tool broad conceptual frame, many studies done afterwards have included only a limited number of variables of Pender's model. Furthermore, Pender's model did not precisely explain the possibilities of direct and indirect paths effects. The objectives of this study are to evaluate Pender's model and thus propose a model that explains health promoting behaviors among late-middle aged women in order to facilitate nursing intervention for this group of population. The hypothetical model was developed based on the Pender's health promoting model and the findings from past studies on women's health. Data were collected by self-reported questionnaires from 417 women living in Seoul, between July and November 1994. Questionnaires were developed based on instruments of Walker and others' health promotion lifestyle profile, Wallston and others' multidimensional health locus of control, Maoz's menopausal symptom check list and Speake and others' health self-rating scale. IN addition, items measuring self-efficacy were made by the present author based on past studies. In a pretest, the questionnaire items were reliable with Cronbach's alpha ranging from .786 to .934. The models for health promoting behaviors were tested by using structural equation modelling technique with LISREL 7.20. The results were summarized as follows : 1. The overall fit of the hypothetical model to the data was good (chi-square=4.42, df=5, p=.490, GFI=.995, AGFI=.962, RMSR=.024). 2. Paths of the model were modified by considering both its theoretical implication and statistical significance of the parameter estimates. Compared to the hypothetical model, the revised model has become parsimonious and had a better fit to the data (chi-square =4.55, df=6, p=.602, GFI=.995, AGFI=.967, RMSR=.024). 3. The results of statistical testing were as follows : 1) Family function internal health locus of control, self-efficacy, and education level exerted significant effects on health promoting behaviors(${\gamma}_{43}$=.272, T=3.714; ${\beta}_[41}$=.211, T=2.797; ${\beta}_{42}$=.199, T=2.717; ${\gamma}_{41}$=.136, T=1.986). The effect of economic status, physical menopausal symptoms, and perceived health status on health promoting behavior were insignificant(${\gamma}_{42}$=.095, T=1.456; ${\gamma}_{44}$=.101, T=1.143; ${\gamma}_{43}$=.082, T=.967). 2) Family function had a significance direct effect on internal health locus of control (${\gamma}_{13}$=.307, T=3.784). The direct effect of education level on internal health locus of control was insignificant(${\gamma}_{11}$=-.006, T=-.081). 3) The directs effects of family functions & internal health locus of control on self-efficacy were significant(${\gamma}_{23}$=.208, T=2.607; ${\beta}_{21}$=.191, T=2.2693). But education level and economic status did not exert a significant effect on self-efficacy(${\gamma}_{21}$=.137, T=1.814; ${\beta}_{22}$=.137, T=1.814; ${\gamma}_{22}$=.112, T=1.499). 4) Education level had a direct and positive effect on perceived health status, but physical menopausal symptoms had a negative effect on perceived health status and these effects were all significant(${\gamma}_{31}$=.171, T=2.496; ${\gamma}_{34}$=.524, T=-7.120). Internal health locus and self-efficacy had an insignificant direct effect on perceived health status(${\beta}_{31}$=.028, T=.363; ${\beta}_{32}$=.041, T=.557). 5) All predictive variables of health promoting behaviors explained 51.8% of the total variance in the model. The above findings show that health promoting behaviors are explained by personal, environmental and perceptual factors : family function, internal health locus of control, self-efficacy, and education level had stronger effects on health promoting behaviors than predictors in the model. A significant effect of family function on health promoting behaviors reflects an important role of the Korean late-middle aged women in family relationships. Therefore, health professionals first need to have a proper evaluation of family function in order to reflect the family function style into nursing interventions and development of strategies. These interventions and strategies will enhance internal health locus of control and self-efficacy for promoting health behaviors. Possible strategies include management of health promoting programs, use of a health information booklets, and individual health counseling, which will enhance internal health locus of control and self-efficacy of the late-middle aged women by making them aware of health responsibilities and value for oneself. In this study, an insignificant effect of physical menopausal symptoms and perceived health status on health promoting behaviors implies that they are not motive factors for health promoting behaviors. Further analytic researches are required to clarify the influence of physical menopausal symptoms and perceived health status on health promoting behaviors with-middle aged women.

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Application of SCAR markers to self-incompatibility genotyping in breeding lines of radish (Raphanus sativus L.)

  • Chung, Hee;Kim, Su;Park, HanYong;Kim, Ki-Taek
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.397-402
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    • 2009
  • Self-incompatibility (SI) prevents self-fertilization by inhibiting the pollen tube growth of self-pollen. Molecular analysis has revealed that the S locus comprises a number of genes, such as the S-locus glycoprotein (SLG), the S-locus receptor kinase (SRK), and SP11 (SCR). Although molecular markers related to those genes have been developed, a simple S-haplotype detecting method has not been reported due to the highly polymorphic and relatively small coding regions. In this study, the sequence characterized amplified region (SCAR) markers were used to establish an efficient radish genotyping method. We identified the S-haplotypes of 192 radish accessions using 19 different markers, which proved to be highly reliable. The accessions were assigned to 17 types of S-haplotypes, including 8 types of SRKs and 9 types of SLGs. Since the developed SCAR markers are based on their gene sequences, we could easily identify the S-haplotypes by a single specific band, with the highest frequencies detected for SLG 5, SRK 1, and SLG 1, in order. Among the tested markers, the SLG 1, SRK 1, and SRK 5 markers exhibited high reliability, compared to phenotypic results. Furthermore, we identified the seven types of unreported SLGs using SLG Class -I and -II specific markers. Although the developed SCAR markers still need to be improved for the genotyping of all S-haplotypes, these markers could be helpful for monitoring inbred lines, and for developing the MAS in radish breeding programs.

A Study of Factors Influencing Health Perception in the Elderly (노인의 건강지각에 영향을 미치는 요인에 관한 연구)

  • Lee, Young-Hee;Choi, Soo-Jeong
    • Journal of Korean Academy of Nursing
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    • v.30 no.4
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    • pp.880-892
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    • 2000
  • The purpose of this study was to investigate the factors influencing health perception in the elderly, to provide the basic data for health behavior program and nursing intervention. The subjects of this study were 240 elderly person over the age 60, living in Seoul and Kangnung. They were conveniently sampled for this study and the data was collected from June 1999 to September 1999. The instruments for this study were the Health Perception Questionaire developed by Ware(1979), the OARS Functional Assessment Questionaire (Duke University 1978), and Multidimensional Health Locus of Control by Wallston, Wallston, and De Vills(1978). The data were analyzed by using SPSS Win computer Program. The results are as follows; 1. The total mean score of the health perception was 52.02(S.D=${\pm}$7.07) in a range of 33 to 69, and the mean score of the functional status was 27.02 (SD=${\pm}$2.75) in a range of zero to 28 and the mean score of the health locus of control was 65.66(S.D=${\pm}$8.68) in a range of 43 to 90. And The mean scores on the HLOC subscales were HLOC-I: 23.73${\pm}$4.56 (range: 6-30), HLOC-P: 23.07${\pm}$4.74 (range: 6-30), HLOC-C: 18.55${\pm}$4.03 (range: 11-30). 2. There was a significant positive correlation between health perception and functional status(r=.216, p=.001), and health perception and the health locus of control(HLOC) were not correlated at the level of statistical significance. However, the HLOC-I and health perception were correlated positively(r=.328, p=.000), and the HLOC-P were correlated negatively (r=-.129, p=.046). 3. There was a significant difference statistically in the degree of health perception according to the age(F=3.351, p=.002), spouse(t=2.232, p=.021), education level(F=7.373, p=.001), disease(t=3.639, p=.000), group activity (t=2.458, p=.015). drink(t=2.327, p=.021). 4. Stepwise multiple regression analysis revealed that the most powerful predictor of health perception was internal health locus of control. A combination of HOLC-I, functional status, HLOC-P, group activity explained 17.9% of the variance for health perception in the elderly. In conclusion, the results of this study show that internal health locus of control factor is very important in explaining the health perception for the elderly. Therefore, it will be considered internal health locus of control factor in nursing intervention and program in order to enforce the health behavior for elderly people .

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