• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.1
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• pp.55-59
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• 2000

A specific and sensitive sandwich enzyme-immunoassay (EIA) using Avidin-Biotin complex was developed for the measurement of GTH II levels in pituitary content and pituitary cell culture medium of the rainbow trout-(Oncorhpchus mykiss). Biotin-salmon GTH II rabbit IgG (sefondary antibody) wai purified by a protein A sepharose affinity chromatography column and that was biotinylated by using Biotin-N-hydroxysuccinimide ofter (BNHS). Non-biotin salmon GTH II rabbit IgG (first antibody) was obtained only through a protein A sepharose affinity chromatography column. The assay was performed by the so-called 'sandwich' method using a microtiter plate, A dose-response curve was obtained between $0.12 to 125 ng/ml$ of salmon GTH II. The displacement curves for pituitary extraction and pituitary cell culture medium of testosterone-treated rainbow trout were Parallel to the standard curie. The intra-assay and inter-assay coefficients of variation (CV) were $8.2{\%} (N=5) and 12.5{\%} (N=6)$, respectively, This assay system was used to measure the amount of GTH II that accumulated in the culture medium of dispersed pituitary cells in testosterone-treated immature rainbow trout, The accumulation was increased with the amount or salmon gonadotropin-releasing hormone. GTH II values determined by the present method were well correlated with those determined by radioimmunoassay. As a result, this assay system was found to be suitable for the measurement of GTH II for pituitary extraction and pituitary culture medium in many salmonid fishes.

• Journal of Animal Science and Technology
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• v.44 no.6
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• pp.661-668
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• 2002

This study was conducted to investigate the effect of environmental factor on milk yield and to estimate lactation curve in Korean cattle. The data for milk yields were collected from 118 cows from 1997 to 2000 at National Livestock Research Institute in Daekwanryoung, Kangwon-do. Average daily milk yields for 1st, 2nd, 3rd and 4th month after calving were 3.74kg, 3.64kg, 3.26kg and 2.99kg. Average daily milk yield for the four months was 3.52kg. The milk yields for cows calved in spring were larger than those calved in fall. Lactation curve of Korean cattle was $y_n$=$2.4845n^{0.1734}e^{－0.0060n}$. Peak milk yield was 3.75kg on 29.03 day after calving. The peak milk yields for multi-parous cows were larger than those of primiparous cows. The peak milk yields for multi-parous cows reached later than those for primiparous cows. The cows calved in spring had higher and earlier peak milk yields than those calved in fall had.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.3
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• pp.238-244
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• 2001

A method of estimating the fluid drag of a fishing gear and otter board spread in a midwater trawl on full scale was described by implementing a three-dimensional semi-analytic treatment of the towing cable (warp) of a trawl system with the field experiments obtained with the SCANMAR system. The shape of hand rope, bridle and float(or ground) rope attached behind otter boards in a horizontal plane was assumed to be of form $y_r=Ax_r^B$. The distance between otter boards (otter board spread) obtained by the three dimensional analysis of a towing cable must be equal to that obtained by the functional equation of the shape of ropes behind otter boards, The angle of attack of ropes which can be obtained from the functional equation enables one to estimate the fluid drag of trawl net (net drag) by subtracting the fluid drag of the hand rope and bridles from the drag component of the tension of hand rope attached just behind the otter boards.

• Journal of Korean Society of Forest Science
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• v.61 no.1
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• pp.8-14
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• 1983

This study was to diagnose the ontogenetic ability of drought tolerance in trees, and was to investigate the seasonal changes in tissue-water relations parameters, ${\pi}_o$, ${\pi}_p$, $E_{max}$, $N_s/DW$, $V_o/W_s$ and $RWC_{(tlp)}$, obtained from P-V curves on the Pinus koraiensis and Abies holophylla shoots. The results obtained are as follows; 1) Seasonal changes of original osmotic pressure at maximum turgot, ${\pi}_o$, were -1.2 to -1.6 MPa in Pinus koraiensis and -1.4 to -1.7 MPa in Abies holophylla. 2) Seasonal changes of osmotic pressure at incipient plasmolysis, ${\pi}_p$, were -1.8 to -2.1 MPa in Pinus koraiensis and -1.6 to -2.1 MPa in Abies holophylla. 3) Seasonal changes of relative water content at incipient plasmolysis, $RWC_{(tlp)}$, were 70 to 77% in Pinus koraiensis and 69 to 85% in Abies holophylla. 4) Seasonal changes of maximum bulk modulus of elasticity at maximum hydration, $E_{max}$, were 2.2 to 6.3 MPa in Pinus koraiensis and 3.1 to 7.9 MPa in Abies holophylla. 5) Seasonal changes of number of osmoles of solute in symplasm versus dry weight, $N_s/DW$, were 0.5 to 1.3 in Pinus koraiensis and 0.3 to 1.0 in Abies holophylla. 6) Seasonal changes of original osmotic water volume versus total water volume (symplasmic and apoplastic water), $V_o/W_s$, were 55 to 65%r in Pinus koraiensis and 40 to 65% in Abies holophylla. Consequently, as the comparative values of ${\pi}_o$, ${\pi}_p$, $E_{max}$, $N_s/DW$, $V_o/W_s$, and $RWC_{(tlp)}$, it might be suggested that Abies holophylla shoot could have more or less a greater ability of drought tolerance as compared with Pinus koraiensis shoot.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.11
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• pp.1667-1674
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• 2010

The fatigue life is estimated while determining the reliability of aircraft structures. In this study, the estimation of fatigue life was carried out on the basis of a cumulative damage theory; the working S-N curve and the equivalent stress on the engine support structure significantly affect the safety of the aircraft. The maximum stress observed was 1,080 MPa in the case of scissors link under crash load condition, and there was a 5% margin for the allowable stress corresponding to the temperature reduction factor. The maximum stress was 876 MPa, and the stress equation coefficient had a maximum value of 0.019 MPa/N in the case of scissors link under fatigue loads. In the results of the fatigue life analysis, the safety life in a fretting area of scissors link upper part was 416,667 flight hour, and other parts showed to infinite life. Therefore, it was demonstrated that the fatigue life requirement of aircraft engine support structure (scissors link, straight link) could be satisfied.

• Journal of the Korean Chemical Society
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• v.18 no.6
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• pp.400-407
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• 1974

Steady-state anodic and cathodic polarization curves were developed for the Al electrode in 60 mole %$AlCl_3$-40 mole % NaCl at $180^{\circ}C$$453^{\circ}K$). Ohmic resistance contributed substantially to the anodic polarization at current densities greater than 50 mA/$CM^2$ even with capillary tip placed close to the electrode. This could not be rationalized from the resistivity of the melt, which would lead to a much smaller polarization. It was therefore concluded that a layer of high resistance $AlCl_3$ (or $AlCl_3$-rich melt) formed close to the anode surface. From the IR-corrected anodic Tafel and Allen-Hickling plots an apparent anodic charge-transfer coefficient of ${\alpha}_a$ = (2.3 RT/F)(d log i/d${\eta}$) = $1.5{\pm}0.25$ was obtained. At cathodic current densities greater than approximately 30 mA/$cm^2$, slow ion diffusion and dendrite growth both interfered with the measurement of kinetic parameters.

• Journal of radiological science and technology
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• v.31 no.3
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• pp.223-228
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• 2008

The most critical point in the medical use of radiation is to minimize the patient's entrance dose while maintaining the diagnostic function. Low-energy photons (long wave X-ray) among diagnostic X-rays are unnecessary because they are mostly absorbed and contribute the increase of patient's entrance dose. The most effective method to eliminate the low-energy photons is to use the filtering plate. The experiments were performed by observing the image quality. The skin entrance dose was 0.3 mmCu (copper) filter. A total of 80 images were prepared as two sets of 40 cuts. In the first set (of 40 cuts), 20 cuts were prepared for the non-filter set and another 20 cuts for the Cu filter of signal + noise image set. In the second set of 40 cuts, 20 cuts were prepared for the non-filter set and another 20 cuts for the Cu filter of non-signal image (noisy image) with random location of diameter 4 mm and 3 mm thickness of acryl disc for ROC signal at the chest phantom. P(S/s) and P(S/n) were calculated and the ROC curve was described in terms of sensitivity and specificity. Accuracy were evaluated after reading by five radiologists. The number of optically observable lesions was counted through ANSI chest phantom and contrast-detail phantom by recommendation of AAPM when non-filter or Cu filter was used, and the skin entrance dose was also measured for both conditions. As the result of the study, when the Cu filter was applied, favorable outcomes were observed on, the ROC Curve was located on the upper left area, sensitivity, accuracy and the number of CD phantom lesions were reasonable. Furthermore, if skin entrance dose was reduced, the use of additional filtration may be required to be considered in many other cases.

• Analytical Science and Technology
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• v.8 no.1
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• pp.1-7
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• 1995

Spectrophotometric determination of U(VI) and Th(IV) by Flow injection method is described. Chrome Azurol S forms water soluble complexes with U(VI) and Th(IV) in the presence of cethyltrimethylammonium bromide. The maximum adsorption of U(VI) and Th(IV) complexes are at 600nm with molar absorptivity of $2.3{\times}10^5Lmol^{-1}cm^{-1}$ and 611nm with molar absorptivity of $3.8{\times}10^5Lmol^{-1}cm^{-1}$ in acetate buffer medium having pH 5.0 and 5.5. The calibration curves of U(VI) and Th(IV) are linear over the range of 0.1~0.8ppm and the correlation coefficients are ca. 0.9960 and 0.9930 respectively. The detection limits(S/N) are 20ppb for U(VI) and 15ppb for Th(IV). The relative standard deviation are ${\pm}1.8%$ for U(VI) and ${\pm}2.1%$ for Th(IV). The sample throughput was ca. $50hr^{-1}$.

• Journal of Ocean Engineering and Technology
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• v.23 no.2
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• pp.74-80
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• 2009

Surface hardening treatments, such as using the high-frequency induction hardening method, are widely used to increase the fatigue life and prevent the failure of materials by locally increasing the surface hardness. This method, in particular, brings an improvement in static strength by compressive residual surface stress due to the hardening. In this study, the mechanical properties of high-frequency induction hardened SCM440 steel were investigated. These results were also compared with those for base metal and a Q/T (tempering after quenching) treatment specimen. The test results showed that partially high-frequency induction hardened SCM440 steel specimens were more improved in static strength, surface hardness, fatigue limit, and anti-wear than the base metal and Q/T treatment specimens. In particular, the fatigue limit of the high-frequency induction hardened SCM440 steel increased by more than about 52% compared to that of base metal and by about 25% compared to that of the Q/T specimen.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.10 no.3
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• pp.13-18
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• 2011

This study deals with the effect of the honing in SCM420H planetary gears. The hardness, tooth profile, lead and roughness of the gear surface can be improved by honing. Therein, the honing techniques are welcomed especially as one of the physical surface improvement methods. As a result, gear honing reduces vibration and noise, and will increase the life of gear. And gear honing also changes the surface of gear tooth in a short time and price, so it is a very important method in manufacturing industry. But nowadays, it is hard to find the detail information of gear honing. The test gear is a planetary gear which is used in the automotive industry. It is manufactured by the hobbing, shaving, carburizing and honing. This study investigates the effect of honing in surface shape of SCM420H planetary gears, including fatigue strength test and characteristics of honing of SCM420H planetary gears.