• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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• pp.33-33
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• 2023

Potatoes are the world's 4th major food crop after maize, rice, and wheat and also are a staple food for 1.3 billion people. Due to their wide adaptability to various environmental conditions, their yeild capacity, and high commercial value, potatoes have contributed to global food security. Many potato germplasms are commonly preserved as whole plants in fields or in storage to maintain their particular genetic combinations. However, field maintenance is expensive and has the risk of potential losses from diseases, pests, plant ageing and climate change. Over the past four decades, meaningful efforts have been made toward the safe long-term conservation of potatoes through cryopreservation methods such as droplet-vitrification. In this study, we tested 4 Korean potato varieties('Golden Egg', 'Golden Ball', 'Ja-Young' and 'Ha-Ryeong') with the modified potato droplet -vitrification protocol. Potato shoot tips are precultured in a sucrose-enriched medium(0.3 and 0.7M for 7 and 17hrs, respectively) and submitted to a loading step with C4 solution for osmoprotection. The treated explants were dehydrated with Plant Vitrification Solution(PVS)2 which is 80% A3 solution in ice for 30 minutes. Thawing and unloading steps were performed with 0.8M sucrose solution for 30 sec(40℃) followed by 30min(25℃, room temperature). In a potato post-culture medium(MS+0.1 mg·L^-1 GA₃+0.1 mg·L^-1 kinetin), we obtained a survival rates of post-thawed explants ranging 16.1-82.2%. The results suggest that modified and optimized protocols are required dependinig on every cultivar, genetic and ecological types. To achieve higher survival and regeneration rates, each step within the cryoprocedure must be carefully optimized.

• The Journal of Korean Physical Therapy
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• 제18권5호
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• pp.13-23
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• 2006

Purpose: This study was to investigate two-point discrimination(TPD) and related factors in rural community residents. Methods: The sample consisted of 68 people who have been living in rural community in June 2005 studied. TPD was measured from the tips of the thumb, index, middle, ring, and little finger of each hand with the TPD esthesiometer. The research was designed to be a cross-sectional measured study. SAS statistical software was used for the analysis. The characteristics of the study sample were described by mean and standard deviation(SD) for continuous variables and by frequency and percentage for categorical variables. The Student's t-test and analysis of variance (ANOVA) were used to compare TPD in gender and diabetes mellitus. A Pearson's correlation analysis was conducted for relationship among values of 5 fingers. Multiple regression analysis was performed to determine the factors associated with TPD. Results: A total of 68 residents were measured, their average $age{\pm}SD$ was $54.2{\pm}23.2$ years(range: $12{\sim}88\;yr$). The mean TPD for 5 fingers tips in dominated side was 4.76mm(thumb 3.98mm, index 4.22mm, middle 4.79mm, ring 5.17mm, and little finger 5.65mm, respectively). 4.91mm for thumb finger of women was significantly higher than 3.54mm that of men's in difference between gender(p=.01l4), also women(4.39mm) was higher than men(3.71mm) in median nerver area(p=.0318). There was a statistically significant difference in age(p=.0022), which were under age of 20(3.36mm), 30 years(3.61mm), 40 years(5.38mm), 50 years(4.84mm), 60 years(5.28mm), 70 years(5.18mm), and over age of 80(5.29mm). Factors related to TPD of fiver fingers by multiple regressions were gender(${\beta}=.03$, p=.0001). Conclusions: Taken together, gender and age were significantly associated with TPD. We recommend that further research should measure TPD by using larger sample sizes and more sensitive measurement instruments.

• The Plant Pathology Journal
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• 제34권1호
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• pp.1-10
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• 2018

A previously unreported bacterial disease on chili pepper (Capsicum annuum L.) seedlings affecting as many as 4% of seedlings was observed in greenhouses in Chihuahua, Mexico (Delicias and Meoqui counties). Initial lesions appeared as irregular small spots on leaves and brown necrosis at margins tips were observed. Later, the spots became necrotic with a chlorotic halo. Advanced disease was associated with defoliation. A Gram negative, rod-shaped bacterium was isolated from diseased chili pepper seedlings. Three inoculation methods revealed that isolated strains produce foliage symptoms, similar to those observed in naturally infected seedlings. Pathogenic strains that caused symptoms in inoculated seedlings were re-isolated and identified to fulfill koch's postulate. Polyphasic approaches for identification including biochemical assays (API 20E and 50CH), carbon source utilization profiling (Biolog) and 16S rDNA, hsp60 and rpoB sequence analysis were done. Enterobacter cloacae was identified as the causal agent of this outbreak on chili pepper seedlings.

• Journal of applied mathematics & informatics
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• 제8권1호
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• pp.59-69
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• 2001

The paper deals with the interaction between three Griffith cracks propagating under antiplane shear stress at the interface of two dissimilar infinite elastic half-spaces. The Fourier transform technique is used to reduce the elastodynamic problem to the solution of a set of integral equations which has been solved by using the finite Hilbert transform technique and Cooke’s result. The analytical expressions for the stress intensity factors at the crack tips are obtained. Numerical values of the interaction efect have been computed for and results show that interaction effects are either shielding or amplification depending on the location of each crack with respect to other and crack tip spacing. AMS Mathematics Subject Classification : 73M25.

• 한국잡초학회지
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• 제18권3호
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• pp.205-213
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• 1998

세계적으로 중요한 식량자원인 감자에 제초제 저항성 형질을 도입한다면, 노지재배가 가능하여 노동력과 인건비를 줄일 수 있을 뿐만 아니라 비닐멀칭 방법의 부산물인 폐비닐에 의한 환경오염을 줄일 수 있는 매우 효과적인 방법이다. 그러나 형질전환식물체의 선발시 일반적으로 사용해온 항생제 내성 표지유전자는 효과적으로 형질전환체를 선발할 수 있는 장점을 가지나 항생제 내성 표지유전자들이 환경중에 노출되었을 때의 안전성 문제, 그리고 일반 소비자들이 항생제 표지유전자를 이용한 형질전환체에 대해 거부감이 느껴지게 할 수 있다는 문제점이 존재한다. 이러한 문제점들은 항생제 내성 표지유전자를 제거하거나 새로운 표지유전자로 대체하는 방법을 사용하여 해결할 수 있을 것으로 생각된다. 따라서 본 실험의 목적은 비선택성 제초제 bialaphos에 저항성을 가지는 phosphinothricin acetyltransferase(PAT) gene을 감자에 도입하는데 있어 항생제 표지 유전자를 사용하지 않는 선발체계를 개발하고자 하였다. 감자 식물체의 재분화는 IBA 0.1mg/L + BA 0.5mg/L를 첨가한 MS배지에서 하였다. 또한 형질전환을 위해 단독 PAT 유전자만을 가지는 pDY502 vector를 재조합하였으며, 재조합된 vector들은 disarmed 된 Agrobacterium MP90에 도입하여 감자의 형질전환에 이용하였다. 형질전환은 강자의 잎과 줄기절편체를 상기 실험에서 재 조합된 vector를 함유한 A. tumefaciens MP90과 공동배양하는 방법으로 수행하였다. PAT 유전자를 표지유전자로 이용하여 bialaphos에 저항성을 가진 감자를 개발하고자 다양한 농도의 bialaphos를 함유한 재분화배지에 감자절편체를 치상하여 내성을 조사한 결과 대조구의 감자절편체가 모두 고사하는 bialaphos 5mg/L를 선발조건으로 하였다. 이와 같은 선발 조건에서 Agrobacterium과 공동 배양한 절편체를 선발배지에 치상한 후 3-4주 정도가 경과하면 shoot가 유기되었으며, 선발배지에서 유기된 shoot의 정단부위를 lcm정도로 잘라 bialaphos가 20mg/L 첨가된 선발배지로 옮겨 2차 선발을 하였다. 형질전환체의 확인은 2차 선발배지에서 선발된 식물체를 대상으로 하였으며, 먼저 PCR반응을 이용하여 도입 유전자의 증폭을 확인하였고, Southern blot을 실시하여 유전자의 도입을 확인하였다. 따라서 PAT 유전자를 감자 형질전환의 표지유전자로 활용할 수 있었으며, 아울러 항생제 표지유전자가 없는 제초제 저항성 형질전환 감자를 획득할 수 있었다.

• 식물조직배양학회지
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• 제28권1호
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• pp.25-32
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• 2001

우리나라 주요 과수류중 하나인 포도의 경정배양을 통하여 증식재료로 이용할 수 있는 다아체의 형성 및 이들의 신장에 미치는 생장조절물질의 영향에 관해 검토하였다. 경정배양 시 재료의 살균은 5월 초순에 생육이 왕성한 신초를 채취하여 NaOCl 1%용액에 10분간 살균한 것에 비해 Wilson액에 5분간 침지 살균했을 때 생존율이 높았다. 다아체의 증식은 3/4 MS 기본배지에 NAA 0.1 mg/L와 BA 3.0mg/L에서 양호하였다. 다아체로부터 신초의 신장은 3/4 MS배지에 AdSO$_4$80 mg/L, NAA 0.1 mg/L와 BA를 1.0~2.0 mg/L 혼합한 배지에서 양호하였으며 품종간 생장조절물질의 적정농도 차이는 거의 없었다.

• 한국균학회지
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• 제1권2호
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• pp.31-33
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• 1973

On September 1972, a blight disease of turf (Arostis canina L.)was found in a golf link in Seoul Country Club at Seoul, Korea. Yellow circular patches of 15-20cm in diameter were observed and spreaded irregulary to collapse of themselves. The roots of the diseased plants were dark in color and were in a final state of decay. We isolated Curvularia sp. from the diseased plants and investigated the morphological and cultural characters. Its characters as follows: Mycelia septate, branched, hyaline or yellowish brown colored. Conidiophores septate, thin brown, unbranched. $65-270{\times}3.5-5{\mu}$. Conidia clustered at the tips of conidiophores, boat form or slightly curved with 1-5 septa, brown; both end cells hyaline; the third cell much larger than the others. $26.1-33.3{\times}9.5-10.1{\mu}$ (4 cell's conidium). The pathogenicity of the fungus to host plant was confirmed by soil inoculation method. We classified the fungus according to Gilman's method as a species Curvularia geniculata (Tracy and Earle) Boedjin.

• 한국작물학회지
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• 제39권5호
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• pp.480-488
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• 1994

White clover는 기존초지에 도입시 정착이 불량한 것이 단점으로 지적되어 왔다. 본시험은 white clover의 발아직후 자엽 및 단엽제거가 건물생산과 형태적 특성에 미치는 영향을 조사하여 기존초지로 도입되는 white clover의 유묘기 관리에 관한 정보를 제공하고자 잎의 크기가 다른 4개 품종(Regal, La. S-1, Huia, S184)을 자엽기에 1개 또는 2개의 자엽을 제거하거나, 단엽기에 단엽, 단엽과 1개의 자엽 또는 단엽과 2개의 자엽을 모두 제거한 후 8주동안 개체당 건물중과 형태적 형질(엽수, 엽면적, 엽병장, 포복경장 및 분지경수)의 변화와 개체당 건물중과 이들 형질과의 관계를 밝히고자 실시하였던 바, 그 결과를 요약하면 다음과 같다. 1. 무탈엽에 비하여 자엽 및 단엽제거는 clover의 개체당 건물중과 형태적 형질을 현저히 감소시켰다. 2. 건물중과 형태적 형질은 단엽기에 단엽과 자엽 제거에 의하여 더욱 감소되고 자엽제거가 많을수록 감소가 큰 것으로 나타났다. 3. 자엽 및 단엽제거 후 생장기간이 증가함으로써 건물중과 형태적 형질이 증가한다 할지라도 포복경장과 분지경수는 처리 6주후부터 급격히 증가하였다. 4. 건물중, 엽면적 및 엽병장은 대엽종일수록 크나, 포복경장, 분지경수 및 엽수는 소엽종일수록 큰 경향을 보였다. 5. 건물중 증가는 엽면적 증가와 밀접한 관련이 있었으나 자엽기에 자엽제거가 단엽기에 단엽과 자엽제거, 그리고 탈엽이 심할수록 증가율(기울기)감소가 큰 것으로 분석되었다.

• Journal of Plant Biology
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• 제21권1_4호
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• pp.33-38
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• 1978

Phosphate absorption from a Na2H32PO4 solution by Oryza sativa L. was studied in order to elucidate kinetic mechanisms of ion transport. The rates of phosphate absorption from different concentraitons indicated the presence of dual mechanisms in root tips, one in the low (1$\times$10-6 to 8$\times$10-5M) and the other in the high (1$\times$10-4 to 8$\times$10-3M). A phosphate compensation point of phosphate transport was revealed with a 1$\times$10-6M solution of Na2H32PO4. The kinetic model that ion transport involves an exchange reaction of absorption and desorptin is prosposed as follows: where C represents an ionic-specific organic carrier in the membrane; M, Mo and Mi are the mineral ions, M-outside and M-inside; MC is a carrier-ion complex; and the K's represent rate constants. In this model, the Mi velocity, v, is given by: {{{{v= {dMi} over {dt}= {(K1K3Mo-K2K4Mi) Ct} over {(K2＋K3)＋K1Mo＋K4Mi} }} where Ct is equal to C＋MC, and t is time.

• The Korean Journal of Ecology
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• 제23권4호
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• pp.323-329
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• 2000

The allelopathic effects of volatile substances from Chamaecyparis obtusa (S. et Z.) Endl. were examined on the germination and seedling growth of some plant species, and on the population growth of some microorganisms. The germination and seedling growth of the receptor plants were suppressed more severely by leaf and fruit essential oils than by those of other parts. Colonial growth of fungi was severely inhibited by essential oils extracted from leaves and fruits. The development of root hairs of the receptor plants was also severely inhibited by the essential oils. The cortical cells at the root tips of Lactuca sativa L. treated with essential oils showed contraction of the cytoplasm, resulting in plasma membranes becoming detached from the cell walls and the cells metamorphosing irregularly. Accumulation of lipid granules inside the contracted cytoplasm and degeneration of mitochondrial cristae were also observed.