• Title/Summary/Keyword: S-246

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n-Alkane Utilizing Capability and Location of the Genes for Alkane Hydroxylases in Pseudomonas maltophilia N246

  • Choi, Soon-Young;Lee, Myung-Hye;Hwang, Moon-Ok
    • Journal of Microbiology and Biotechnology
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    • v.3 no.4
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    • pp.252-255
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    • 1993
  • Pseudomonas maltophilia N246 carrying on OCT plasmid grew on n-alkanes of 6 to 14 carbon atoms, but not on n-alkanes of more carbon atoms. P. maltophilia strains with and without OCT plasmid could utilize primary alcohols. aldehydes and fatty acids derived from n-alkane. The N246 strain could also utilize monocarboxylic and dicarboxylic acids, and terminal branched dimethyloctane. Unlike the genes of alcohol dehydrogenase and aldehyde dehydrogenase which were located on both the chromosome and the OCT plasmid, genes for the alkane hydroxylase components were located only on the OCT plasmid in P. maltophilia N246.

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DPSS laser를 이용한 비정질 칼코게나이드 박막의 회절격자 형성

  • Nam, Gi-Hyeon;Jeong, Won-Guk;Jeong, Hong-Bae
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.246-246
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    • 2010
  • In this paper, we investigated the diffraction grating efficiency on AsSeS and Ag-doped amorphous chalcogenide Ag/AsSeS thin film for used to volume hologram. The Chalcogenide film thickness was 0.5um and Ag thin film was varied from 10nm and 20nm. Diffraction efficiency was obtained from (P:P) polarized Diode Pumped Solid State laser(DPSS, 532.0nm: 200mW) beam on AsSeS and Ag/AsSeS thin films. As a results, diffraction grating was not formed at AsSeS thin film but at Ag-doped AsSeS thin film, diffraction grating was formed well compare with the former.

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Blockchain-based ticket trading system (블록체인 기반 티켓 거래 시스템)

  • Seong-Eun Park;Yeon-Hui Park;Joo-Hyun Lee;Seon-Jeong Heo
    • Proceedings of the Korea Information Processing Society Conference
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    • 2023.05a
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    • pp.246-247
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    • 2023
  • 최근 문화 공연 수요의 증가에 따라 암표 거래도 증가하는 추세이다. 이를 해결하고자 본 논문은 블록체인 기반 티켓 거래 시스템을 제시한다. 메타마스크로 티켓 거래를 진행하며 블록체인에 거래 내역을 기록함으로써 악의적인 재판매 행위를 근절하는데 목적이 있다. 블록체인 기술을 활용해 티켓 거래 내역의 위변조 위험성을 낮추고 무결성을 보장하여 기존 티켓 거래 사이트보다 높은 신뢰성을 제공한다. 또한 티켓 재판매 시 가격을 원가 이하로 제한하고 양도자의 지갑 주소를 양수자에게 비공개하여 사이트 외 추가적인 불법 거래를 방지한다. 본 연구가 공정한 디지털 거래 환경을 조성할 것이라 기대한다.

Phosphorylation of $Ser^{246}$ Residue in Integrin-linked Kinase 1 by Serum- and Glucocorticoid-induced Kinase 1 is Required to Form a Protein-protein Complex with 14-3-3

  • Chun, Jae-Sun;Kang, Sang-Sun
    • Animal cells and systems
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    • v.9 no.3
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    • pp.161-171
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    • 2005
  • Integrin-linked kinase 1 (ILK1) regulates several protein kinases, including PKB/Akt kinase and glycogen synthase kinase ${\beta}$. ILK1 is also involved distinctively in the cell morphological and structural functions by interacting with the components of the extracellular matrix or integrin. According to the information of serum- and glucocorticoid-induced kinase 1 (SGK1) substrate specificity (R-X-R-X-X(S/T)-${\phi};{\phi}$ indicates a hydrophobic amino acid), two putative phosphorylation sites, $Thr^{181}\;and\;Ser^{246}$, were found in ILK1. We showed that ILK1 fusion protein and two fluorescein-labeled ILK1 peptides, $FITC-^{174}RTRPRNGTLN^{183}$ and $FITC-^{239}CPRLRIFSHP^{248}$, were phosphorylated by SGK1 in vitro. We also identified that 14-3-3 ${\theta}\;{\varepsilon}\;and\;{\xi}$, among several 143-3 isotypes $({\beta},\;{\gamma},\;{\varepsilon},\;{\eta},\;{\sigma},\;{\theta},\;{\tau}\;and\;{\xi})$ formed protein complex with ILK1 in COS-1 cells. Furthermore, the phosphorylation of $Ser^{246}$ by SGK1 induced the binding with 14-3-3. It was also demonstrated that 14-3-3-bound ILK1 has reduced kinase activity. Thus, these data suggest that SGK1 phosphorylates $Thr^{181}\;and\;Ser^{246}$ of ILK1 and the phosphorylation of its $Ser^{246}$ makes ILK1 bind to 14-3-3, resulting in the inhibition of ILK1 kinase activity.