• Title/Summary/Keyword: S-100 standard

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Herbicidal Phytotoxicity under Adverse Environments and Countermeasures (불량환경하(不良環境下)에서의 제초제(除草劑) 약해(藥害)와 경감기술(輕減技術))

  • Kwon, Y.W.;Hwang, H.S.;Kang, B.H.
    • Korean Journal of Weed Science
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    • v.13 no.4
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    • pp.210-233
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    • 1993
  • The herbicide has become indispensable as much as nitrogen fertilizer in Korean agriculture from 1970 onwards. It is estimated that in 1991 more than 40 herbicides were registered for rice crop and treated to an area 1.41 times the rice acreage ; more than 30 herbicides were registered for field crops and treated to 89% of the crop area ; the treatment acreage of 3 non-selective foliar-applied herbicides reached 2,555 thousand hectares. During the last 25 years herbicides have benefited the Korean farmers substantially in labor, cost and time of farming. Any herbicide which causes crop injury in ordinary uses is not allowed to register in most country. Herbicides, however, can cause crop injury more or less when they are misused, abused or used under adverse environments. The herbicide use more than 100% of crop acreage means an increased probability of which herbicides are used wrong or under adverse situation. This is true as evidenced by that about 25% of farmers have experienced the herbicide caused crop injury more than once during last 10 years on authors' nationwide surveys in 1992 and 1993 ; one-half of the injury incidences were with crop yield loss greater than 10%. Crop injury caused by herbicide had not occurred to a serious extent in the 1960s when the herbicides fewer than 5 were used by farmers to the field less than 12% of total acreage. Farmers ascribed about 53% of the herbicidal injury incidences at their fields to their misuses such as overdose, careless or improper application, off-time application or wrong choice of the herbicide, etc. While 47% of the incidences were mainly due to adverse natural conditions. Such misuses can be reduced to a minimum through enhanced education/extension services for right uses and, although undesirable, increased farmers' experiences of phytotoxicity. The most difficult primary problem arises from lack of countermeasures for farmers to cope with various adverse environmental conditions. At present almost all the herbicides have"Do not use!" instructions on label to avoid crop injury under adverse environments. These "Do not use!" situations Include sandy, highly percolating, or infertile soils, cool water gushing paddy, poorly draining paddy, terraced paddy, too wet or dry soils, days of abnormally cool or high air temperature, etc. Meanwhile, the cultivated lands are under poor conditions : the average organic matter content ranges 2.5 to 2.8% in paddy soil and 2.0 to 2.6% in upland soil ; the canon exchange capacity ranges 8 to 12 m.e. ; approximately 43% of paddy and 56% of upland are of sandy to sandy gravel soil ; only 42% of paddy and 16% of upland fields are on flat land. The present situation would mean that about 40 to 50% of soil applied herbicides are used on the field where the label instructs "Do not use!". Yet no positive effort has been made for 25 years long by government or companies to develop countermeasures. It is a really sophisticated social problem. In the 1960s and 1970s a subside program to incoporate hillside red clayish soil into sandy paddy as well as campaign for increased application of compost to the field had been operating. Yet majority of the sandy soils remains sandy and the program and campaign had been stopped. With regard to this sandy soil problem the authors have developed a method of "split application of a herbicide onto sandy soil field". A model case study has been carried out with success and is introduced with key procedure in this paper. Climate is variable in its nature. Among the climatic components sudden fall or rise in temperature is hardly avoidable for a crop plant. Our spring air temperature fluctuates so much ; for example, the daily mean air temperature of Inchon city varied from 6.31 to $16.81^{\circ}C$ on April 20, early seeding time of crops, within${\times}$2Sd range of 30 year records. Seeding early in season means an increased liability to phytotoxicity, and this will be more evident in direct water-seeding of rice. About 20% of farmers depend on the cold underground-water pumped for rice irrigation. If the well is deep over 70m, the fresh water may be about $10^{\circ}C$ cold. The water should be warmed to about $20^{\circ}C$ before irrigation. This is not so practiced well by farmers. In addition to the forementioned adverse conditions there exist many other aspects to be amended. Among them the worst for liquid spray type herbicides is almost total lacking in proper knowledge of nozzle types and concern with even spray by the administrative, rural extension officers, company and farmers. Even not available in the market are the nozzles and sprayers appropriate for herbicides spray. Most people perceive all the pesticide sprayers same and concern much with the speed and easiness of spray, not with correct spray. There exist many points to be improved to minimize herbicidal phytotoxicity in Korea and many ways to achieve the goal. First of all it is suggested that 1) the present evaluation of a new herbicide at standard and double doses in registration trials is to be an evaluation for standard, double and triple doses to exploit the response slope in making decision for approval and recommendation of different dose for different situation on label, 2) the government is to recognize the facts and nature of the present problem to correct the present misperceptions and to develop an appropriate national program for improvement of soil conditions, spray equipment, extention manpower and services, 3) the researchers are to enhance researches on the countermeasures and 4) the herbicide makers/dealers are to correct their misperceptions and policy for sales, to develop database on the detailed use conditions of consumer one by one and to serve the consumers with direct counsel based on the database.

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Studies on the Estimation of Leaf Production in Mulberry Trees 1. Estimation of the leaf production by leaf area determination (상엽 수확고 측정에 관한 연구 - 제1보 엽면적에 의한 상엽량의 순서 -)

  • 한경수;장권열;안정준
    • Journal of Sericultural and Entomological Science
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    • v.8
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    • pp.11-25
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    • 1968
  • Various formulae for estimation of leaf production in mulberry trees were investigated and obtained. Four varieties of mulberry trees were used as the materials, and seven characters namely branch length. branch diameter, node number per branch, total branch weight, branch weight except leaves, leaf weight and leaf area, were studied. The formulae to estimate the leaf yield of mulberry trees are as follows: 1. Varietal differences were appeared in means, variances, standard devitations and standard errors of seven characters studied as shown in table 1. 2. Y$_1$=a$_1$X$_1$${\times}$P$_1$......(l) where Y$_1$ means yield per l0a by branch number and leaf weight determination. a$_1$.........leaf weight per branch. X$_1$.......branch number per plant. P$_1$........plant number per l0a. 3. Y$_2$=(a$_2$${\pm}$S. E.${\times}$X$_2$)+P$_1$.......(2) where Y$_2$ means leaf yield per l0a by branch length and leaf weight determination. a$_2$......leaf weight per meter of branch length. S. E. ......standard error. X$_2$....total branch length per plant. P$_1$........plant number per l0a as written above. 4. Y$_3$=(a$_3$${\pm}$S. E${\times}$X$_3$)${\times}$P$_1$.....(3) where Y$_3$ means of yield per l0a by branch diameter measurement. a$_3$.......leaf weight per 1cm of branch diameter. X$_3$......total branch diameter per plant. 5. Y$_4$=(a$_4$${\pm}$S. E.${\times}$X$_4$)P$_1$......(4) where Y$_4$ means leaf yield per 10a by node number determination. a$_4$.......leaf weight per node X$_4$.....total node number per plant. 6. Y$\sub$5/= {(a$\sub$5/${\pm}$S. E.${\times}$X$_2$)Kv}${\times}$P$_1$.......(5) where Y$\sub$5/ means leaf yield per l0a by branch length and leaf area measurement. a$\sub$5/......leaf area per 1 meter of branch length. K$\sub$v/......leaf weight per 100$\textrm{cm}^2$ of leaf area. 7. Y$\sub$6/={(X$_2$$\div$a$\sub$6/${\pm}$S. E.)}${\times}$K$\sub$v/${\times}$P$_1$......(6) where Y$\sub$6/ means leaf yield estimated by leaf area and branch length measurement. a$\sub$6/......branch length per l00$\textrm{cm}^2$ of leaf area. X$_2$, K$\sub$v/ and P$_1$ are written above. 8. Y$\sub$7/= {(a$\sub$7/${\pm}$S. E. ${\times}$X$_3$)}${\times}$K$\sub$v/${\times}$P$_1$.......(7) where Y$\sub$7/ means leaf yield estimates by branch diameter and leaf area measurement. a$\sub$7/......leaf area per lcm of branch diameter. X$_3$, K$\sub$v/ and P$_1$ are written above. 9. Y$\sub$8/= {(X$_3$$\div$a$\sub$8/${\pm}$S. E.)}${\times}$K$\sub$v/${\times}$P$_1$.......(8) where Y$\sub$8/ means leaf yield estimates by leaf area branch diameter. a$\sub$8/......branch diameter per l00$\textrm{cm}^2$ of leaf area. X$_3$, K$\sub$v/, P$_1$ are written above. 10. Y$\sub$9/= {(a$\sub$9/${\pm}$S. E.${\times}$X$_4$)${\times}$K$\sub$v/}${\times}$P$_1$......(9) where Y$\sub$7/ means leaf yield estimates by node number and leaf measurement. a$\sub$9/......leaf area per node of branch. X$_4$, K$\sub$v/, P$_1$ are written above. 11. Y$\sub$10/= {(X$_4$$\div$a$\sub$10/$\div$S. E.)${\times}$K$\sub$v/}${\times}$P$_1$.......(10) where Y$\sub$10/ means leaf yield estimates by leaf area and node number determination. a$\sub$10/.....node number per l00$\textrm{cm}^2$ of leaf area. X$_4$, K$\sub$v/, P$_1$ are written above. Among many estimation methods. estimation method by the branch is the better than the methods by the measurement of node number and branch diameter. Estimation method, by branch length and leaf area determination, by formulae (6), could be the best method to determine the leaf yield of mulberry trees without destroying the leaves and without weighting the leaves of mulberry trees.

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Physicochemical Properties of Various Blends of Peatmoss and Perlite and the Selection of Rooting Media for Different Growing Seasons (다양한 종류의 피트모스와 펄라이트 혼합에 따른 물리·화학성 변화와 계절별 육묘를 위한 상토 선발)

  • Shim, Chang Yong;Kim, Chang Hyeon;Park, In Sook;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.34 no.6
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    • pp.886-897
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    • 2016
  • The physical properties of rooting media for the establishment of plugs in a greenhouse are modified according to variations in the greenhouse environment throughout the season. In this study, we established a standard for rooting media for the production of plug seedlings for each growing season (summer, winter and spring fall). Eight types of peatmoss (PM) and 4 types of perlite (PL) commonly used in Korea were collected and blended with the ratio of 7 parts PM to 3 parts PL (v/v) to make 32 different rooting media blends. We determined the total porosity (TP), container capacity (CC), air-filled porosity (AFP), pH, and electrical conductivity (EC) of the 32 media blends, and 6 media blends were selected for seasonal use. We also conducted additional analyses for plant easily available water (EAW), buffering water (BW), cation exchange capacity (CEC), and nutrient contents in the 6 media blends. The TP, CC, and AFP of the 32 media blends ranged from 64.7 to 96.0%, 42.9 to 90.1%, and 1.3 to 27.8%, respectively, indicating that the physical properties were strongly influenced by the type of PM and PL. The pH and EC of the PMs ranged from 2.96 to 3.81 and 0.08 to $0.47dS{\cdot}m^{-1}$, respectively. However, after blending the PM with the PL the pH was raised and the EC was lowered The media blends selected for the summer growing season were Blonde Golden peatmoss (BG) + No. 1 perlite size < 1 mm (PE1) and Latagro 0-10 mm (L1) + No. 2 perlite size 1-2 mm (PE2). These two media blends had 89.8-90.9% of TP, 80.8-81.3% of CC, and 9.0-9.7% of AFP. The media blends selected for the winter growing season were Sfagnumi Turvas (ST) + PE2 and Latagro 20-40 mm (L3) + PE2. These media blends had 79.9-86.7% of TP, 60.4-74.9% of CC, and 11.8-19.6% of AFP. The TP, CC, and AFP of two media blends, BG + No.3 perlite 2-5 mm (PE3) and Orange peatmoss (O) + PE3, selected for the spring and fall growing seasons, respectively, were 85.2-87.3%, 77.9%, and 7.4-9.4%, respectively. The percentage of EAW of the media blends selected for the spring, summer, and winter growing seasons ranged from 24.2-24.9%, 22.0-28.6%, and 18.0-21.8%, respectively, but the percentages of BW were not significantly different among the selected root media blends. The pH, EC, and CEC of the 6 selected media blends ranged from 3.11-3.97, $0.06-0.26dS{\cdot}m^{-1}$, and $97-119meq{\cdot}100g^{-1}$, respectively.

IN VITRO EVALUATION OF THE CONSISTENCY OF TWO ELECTRONIC APEX LOCATORS (2종 전자근관장측정기의 일관성에 관한 in vitro 연구)

  • Hwang, Gyu-Young;Roh, Byoung-Duck;Kim, Eui-Sung;Lee, Seung-Jong
    • Restorative Dentistry and Endodontics
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    • v.33 no.1
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    • pp.20-27
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    • 2008
  • The purpose of this study was to evaluate the consistency of two electronic apex locators in vitro model. Materials consisted of fifty two extracted premolars and two electronic apex locators; Root ZX (J. Morita, Osaka, Japan) and E-Magic Finder Deluxe (S-Denti. Cheonan, Korea). After access preparation, the teeth were embedded in a saline-mixed alginate model. Canal lengths of each tooth were measured at "0.5" and "Apex" mark of the apex locators, respectively so that each tooth had two measurements from 0.5 and Apex points. The file was fixed at final measurement using a glass ionomer cement. The apical 4 mm from the apex was exposed to measure the distance from the file tip to the major apical foramen of each tooth. Average distances and standard deviations were used to evaluate the consistency. Results showed that all measurements of both Root ZX and E-Magic Finder located the major foramen the range of ${\pm}0.5\;mm$ level. Both apex locators showed better consistency at Apex mark than at 0.5 mark. The average distance of file tip-major foramen was - 0.18 mm at 0.5 mark and - 0.07 mm at Apex mark in Root ZX, - 0.25 mm at 0.5 mark and - 0.02 mm at Apex mark in E-Magic Finder. Standard deviation was 0.21 at 0.5 mark and 0.12 at Apex mark in Root ZX, 0.12 at 0.5 mark and 0.09 at Apex mark in E-Magic Finder.

Microbiological Hazard Analysis of Foods in Large Western-style Restaurants (대형 양식당 음식에 대한 미생물학적 위해분석)

  • Nam, Eun-Jeong;Kang, Young-Jae;Lee, Yeon-Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.8
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    • pp.1049-1057
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    • 2008
  • The purpose of this study was to microbiologically evaluate common foods and preparation processes in three large (over 100 pyeong) western-style restaurants in Daegu. Microbiological testing was conducted for pathogens including E. coli, Salmonella spp., Listeria monocytogenes, Staphylococcus aureus, E. coli O157:H7, Vibrio parahaemolyticus, and Bacillus cereus, as well as total plate count and coliforms. Food samples selected for testing represent common menu items and preparation processes. These samples included: 1) hamburger steak (cooking process), 2) mashed potatoes (reheating process), and 3) salad (non-heating process). The results showed that E. coli and S. aureus were detected in the raw ground beef used to prepare hamburger steak, but they were not detected after cooking at $82{\sim}86^{\circ}C$, 10 min. Microbiological quality of the mashed potatoes was better after reheating than during storage in a refrigerator after cooking. Total plate count and coliforms increased, and E. coli was detected in the salad ingredients stored in the refrigerator before serving. In order to serve foods of good microbiological safety and quality in western-style restaurants, standard cooking and reheating temperatures must be maintained during the cooking and reheating processes, and sanitation standard operating procedures must be followed for washing and storage of non-heated and refrigerated menu items.

A Study on Significance Testing of Driver's Visual Behavior due to the VMS Message Display Forms on the Road (도로상 VMS 표출방식별 운전자 유의성 검증에 관한 연구)

  • Kum, Ki-Jung;Son, Young-Tae;Bae, Deok-Mo;Son, Seung-Neo
    • International Journal of Highway Engineering
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    • v.7 no.4 s.26
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    • pp.151-162
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    • 2005
  • Variable Message Sign (VMS), which provides drivers with direct information about state of traffic congestion and for prevent an accident, is the most effective method among the methods of providing information in Advanced Transportation Management System. Currently establishment and the VMS which is operated foundation lets in Guidelines on the use of Variable message sign (a book of the VMS) of 1999 November the Ministry Construction & Transportation, these contents mean main viewpoint on physical part such as message special quality variable (font, character size and line space, word interval) and position mainly among standard about establishment in general. But, it is true that using without effect verification on the character of VMS display and that using mode of stationary-centered. In this paper, it executed significance test to effort verification on the character of VMS display for more practical and effective information transmission based on the driver viewpoint For the researches; develop 3D-Simulation, select characteristics of driver's visual cognition behavior (the conspicuity, the legibility and the comprehensibility), evaluation each issue (day or night, 80km/h or 100km/h). Especially, that used the Eye Marker Recorder to measure of reading-time (legibility) thus, confirmed objectivity and reduce an observational error. The results showed that the conspicuity is Flashing> Stationary>Scroll. The legibility is not deference that Flashing between stationary form. Also the comprehensibility result showed that Flashing> Stationary>Stroll form.

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THE LUMINOSITY-LINEWIDTH RELATION AS A PROBE OF THE EVOLUTION OF FIELD GALAXIES

  • GUHATHAKURTA PURAGRA;ING KRISTINE;RIX HANS-WALTER;COLLESS MATTHEW;WILLIAMS TED
    • Journal of The Korean Astronomical Society
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    • v.29 no.spc1
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    • pp.63-64
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    • 1996
  • The nature of distant faint blue field galaxies remains a mystery, despite the fact that much attention has been devoted to this subject in the last decade. Galaxy counts, particularly those in the optical and near ultraviolet bandpasses, have been demonstrated to be well in excess of those expected in the 'no-evolution' scenario. This has usually been taken to imply that galaxies were brighter in the past, presumably due to a higher rate of star formation. More recently, redshift surveys of galaxies as faint as B$\~$24 have shown that the mean redshift of faint blue galaxies is lower than that predicted by standard evolutionary models (de-signed to fit the galaxy counts). The galaxy number count data and redshift data suggest that evolutionary effects are most prominent at the faint end of the galaxy luminosity function. While these data constrain the form of evolution of the overall luminosity function, they do not constrain evolution in individual galaxies. We are carrying out a series of observations as part of a long-term program aimed at a better understanding of the nature and amount of luminosity evolution in individual galaxies. Our study uses the luminosity-linewidth relation (Tully-Fisher relation) for disk galaxies as a tool to study luminosity evolution. Several studies of a related nature are being carried out by other groups. A specific experiment to test a 'no-evolution' hypothesis is presented here. We have used the AUTOFIB multifibre spectro-graph on the 4-metre Anglo-Australian Telescope (AAT) and the Rutgers Fabry-Perot imager on the Cerro Tolalo lnteramerican Observatory (CTIO) 4-metre tele-scope to measure the internal kinematics of a representative sample of faint blue field galaxies in the red-shift range z = 0.15-0.4. The emission line profiles of [OII] and [OIII] in a typical sample galaxy are significantly broader than the instrumental resolution (100-120 km $s^{-l}$), and it is possible to make a reliable de-termination of the linewidth. Detailed and realistic simulations based on the properties of nearby, low-luminosity spirals are used to convert the measured linewidth into an estimate of the characteristic rotation speed, making statistical corrections for the effects of inclination, non-uniform distribution of ionized gas, rotation curve shape, finite fibre aperture, etc.. The (corrected) mean characteristic rotation speed for our distant galaxy sample is compared to the mean rotation speed of local galaxies of comparable blue luminosity and colour. The typical galaxy in our distant sample has a B-band luminosity of about 0.25 L$\ast$ and a colour that corresponds to the Sb-Sd/Im range of Hub-ble types. Details of the AUTOFIB fibre spectroscopic study are described by Rix et al. (1996). Follow-up deep near infrared imaging with the 10-metre Keck tele-scope+ NIRC combination and high angular resolution imaging with the Hubble Space Telescope's WFPC2 are being used to determine the structural and orientation parameters of galaxies on an individual basis. This information is being combined with the spatially resolved CTIO Fabry-Perot data to study the internal kinematics of distant galaxies (Ing et al. 1996). The two main questions addressed by these (preliminary studies) are: 1. Do galaxies of a given luminosity and colour have the same characteristic rotation speed in the distant and local Universe? The distant galaxies in our AUTOFIB sample have a mean characteristic rotation speed of $\~$70 km $s^{-l}$ after correction for measurement bias (Fig. 1); this is inconsistent with the characteristic rotation speed of local galaxies of comparable photometric proper-ties (105 km $s^{-l}$) at the > $99\%$ significance level (Fig. 2). A straightforward explanation for this discrepancy is that faint blue galaxies were about 1-1.5 mag brighter (in the B band) at z $\~$ 0.25 than their present-day counterparts. 2. What is the nature of the internal kinematics of faint field galaxies? The linewidths of these faint galaxies appear to be dominated by the global disk rotation. The larger galaxies in our sample are about 2"-.5" in diameter so one can get direct insight into the nature of their internal velocity field from the $\~$ I" seeing CTIO Fabry-Perot data. A montage of Fabry-Perot data is shown in Fig. 3. The linewidths are too large (by. $5\sigma$) to be caused by turbulence in giant HII regions.

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Detection of Copy Number Variation of the KIT Gene in the Landrace Breed using an Quantitative Oligonucleotide Ligation Assay(qOLA) (Quantitative Oligonucleotide Ligation Assay(qOLA)를 이용한 Landrace 품종의 KIT 유전자 반복수 변이 탐지)

  • Seo, B.Y.;Kim, J.H.;Nahm, D.W.;Yoo, C.K.;Lee, S.H.;Lee, J.B.;Lim, H.T.;Jung, E.J.;Cho, I.C.;Heo, K.N.;Jeon, J.T.
    • Journal of Animal Science and Technology
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    • v.49 no.5
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    • pp.559-568
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    • 2007
  • Recently, copy number variations (CNV) of genes or genomic segments have been intensively studied and various analysis methods have been developed. In this study, quantitative oligonucleotide ligation assay (qOLA) was applied to investigate CNV of KIT gene in the Landrace breed. A combined assay using qOLA and pyrosequencing, 6 genotype classes, I1/I1 or I3/i (IBe), I1/I2 or I3/IP, I1/I3, I1/IP or I2/i (IBe), I2/I2and I2/IP, were identified from 44 Landrace pigs. Genotype assignment using grouping features of measurements on a scatter plot showed 100% agreement with those using a statistical assignment by PROC FASTCLUS procedure implemented in the SAS package. Two versions (3100 and 3130) of ABI sequencers gave the same genotyping results, indicating there was no influence on qOLA by different versions of instrument, however, the means of standard deviation and coefficient of variation from the qOLA on a ABI 3130 (2.33 and 4.10) was lower than those from the qOLA on a ABI 3100 (2.67 and 4.81). Effect of proteinase K treatment on the PCR product followed by qOLA was very clear because noise peaks were disappeared and the observed ration fit better to the reference ratio corresponding to each genotype.

Optimum Size Selection and Machinery Costs Analysis for Farm Machinery Systems - Programming for Personal Computer - (농기계(農機械) 투입모형(投入模型) 설정(設定) 및 기계이용(機械利用) 비용(費用) 분석연구(分析硏究) - PC용(用) 프로그램 개발(開發) -)

  • Lee, W.Y.;Kim, S.R.;Jung, D.H.;Chang, D.I.;Lee, D.H.;Kim, Y.H.
    • Journal of Biosystems Engineering
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    • v.16 no.4
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    • pp.384-398
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    • 1991
  • A computer program was developed to select the optimum size of farm machine and analyze its operation costs according to various farming conditions. It was written in FORTRAN 77 and BASIC languages and can be run on any personal computer having Korean Standard Complete Type and Korean Language Code. The program was developed as a user-friendly type so that users can carry out easily the costs analysis for the whole farm work or respective operation in rice production, and for plowing, rotarying and pest controlling in upland. The program can analyze simultaneously three different machines in plowing & rotarying and two machines in transplanting, pest controlling and harvesting operations. The input data are the sizes of arable lands, possible working days and number of laborers during the opimum working period, and custom rates varying depending on regions and individual farming conditions. We can find out the results such as the selected optimum combination farm machines, the overs and shorts of working days relative to the planned working period, capacities of the machines, break-even points by custom rate, fixed costs for a month, and utilization costs in a hectare.

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Effect of Foodservice Employee's Hand Hygiene Improvement according to Food Safety Education (위생교육 실시에 따른 조리종사원의 손 위생 개선 효과)

  • Cho, Hyun-Ok;Bae, Hyun-Joo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.2
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    • pp.284-292
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    • 2016
  • The purpose of this study was to analyze the personal hygiene status of foodservice employees as well as microbiological counts of their hands according to food safety education. A survey was conducted for employees who worked at five foodservice facilities, and the results of the survey were assessed using the SAS program (ver. 9.3). A total of 20 employees' hands were sampled to analyze aerobic plate counts (APCs), coliforms counts (CCs), and Staphylococcus aureus. The food safety education program was composed of four education sessions, including microbiological evaluation of employees' hands. According to the survey results, all employees wore a sanitary uniform, hair cap, and shoes. However, 96% of employees used the restroom wearing sanitary shoes, whereas just 20% of them washed their hands using the standard method. Additionally, employees washed hands after using the restroom (100%), before using single-use gloves (76%), after eating (76%). The results of the microbiological hazards analysis of foodservice employees' hands showed that the average numbers of APCs and CCs before cooking were 5.53 log CFU/hand and 2.95 log CFU/hand respectively, whereas the average numbers of APCs and CCs while cooking at foodservice facilities were 6.49 log CFU/hand and 3.29 log CFU/hand, respectively. Staphylococcus aureus was detected in 10% of their hands before cooking and 5% of their hands while cooking. The average numbers of APCs and CCs were significantly reduced before cooking as well as while cooking, and Staphylococcus aureus was not detected in any of the samples as the frequency of food safety education increased. In conclusion, this study shows that it is necessary for foodservice employees to regularly participate in food safety education with effective instruments in order to improve their hygiene level.