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Bioconcentration Factor(BCF) of Perchlorate from Agricultural Products and Soils (농산물과 토양에 대한 퍼클로레이트 함량 평가 및 생물농축계수 산출)

  • Kim, Ji-Young;Kim, Min-Ji;Lee, Jeong-Mi;Kim, Doo-Ho;Park, Ki-Moon;Kim, Won-Il
    • Korean Journal of Environmental Agriculture
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    • v.32 no.3
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    • pp.224-230
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    • 2013
  • BACKGROUND: Perchlorate(${ClO_4}^-$) is an anion that is extremely water-soluble and environmentally stable. It mostly exists in the form of sodium perchlorate, ammonium perchlorate and potassium perchlorate which are used in rocket fuels, propellants, ignitable sources, air bag inflation systems and explosives. Perchlorate can be taken into the thyroid glands and interfere with iodide uptake. The determination of perchlorate in agricultural products is important due to its potential health impact on humans. The objective of this study was to determine the perchlorate concentrations in the samples of various agricultural products and soils. METHODS AND RESULTS: In this study, samples of cereal(Rice, Barley, Corn, Bean), vegetable(Spinach, Lettuce, Sesame, Chives, Chili, Pumpkin, Tomato), fruit(Apple, Pear, Tangerine, Grape) were analyzed for perchlorate contents. Perchlorate concentrations were analyzed by liquid chromatography-tandem mass spectrometry. The results showed that agricultural products respectively contained perchlorate concentrations in the range of : cereals N.D.~$7.46{\mu}g/kg$, vegetables $0.52{\sim}23.06{\mu}g/kg$, fruits $0.19{\sim}2.66{\mu}g/kg$. Bioconcentration factor was in the order of : vegetables > cereals > fruits. Bioconcentration factor was highest follwed by Sesame 37.88, Corn 21.51, Spinach 10.57, Tangerine 4.39, Chives 2.89 and Lettuce 1.90. The recoveries of perchlorate from spiked agricultural products and soils ranged from 87.72~111.26% and 102.09~111.23%. CONCLUSION(S): The health risk assessment results obtained in this study are lower than the RfD(Reference Dose, 0.0007 mg/kg/body weight/day) value as suggested by the Integrated Risk Information System(US IRIS). Our results indicate that, people currently exposed to perchlorate from agricultural products consumption are considered as safe.

Community Structure of Macrobenthic Assemblages around the Wolseong Nuclear Power Plant, East Sea of Korea (월성 원자력발전소 주변해역에 서식하는 대형저서동물의 군집구조)

  • Seo, In-Soo;Moon, Hyung-Tae;Choi, Byoung-Mi;Kim, Mi-Hyang;Kim, Dae-Ik;Yun, Jae-Seong;Byun, Ju-Young;Choi, Hue-Chang;Son, Min-Ho
    • Korean Journal of Environmental Biology
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    • v.27 no.4
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    • pp.341-352
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    • 2009
  • This study was carried out to investigated community structure of macrobenthic assemblages around the Wolseong Nuclear Power Plant, East Sea of Korea and seasonal sampling was performed from October 2007 to July 2008. A total of 163 macrobenthic fauna were collected. The overall average macrobenthos density and biomass were 1,005 individuals $m^{-2}$ and $21.81\;gWWt\;m^{-2}$, respectively. Based on the LeBris (1988) index, there were 10 dominant species accounting for approximately 69.00% of total individuals. The major dominant species were the polychaetes Spiophanes bombyx (349 inds. $m^{-2}$), Mediomastus californiensis (82 inds. $m^{-2}$), Sigambra tentaculata (55 inds. $m^{-2}$), Magelona japonica (50 inds. $m^{-2}$), Scoletoma longifolia (33 inds. $m^{-2}$) and the Unidentified amphipod (Amphipoda spp., 72 inds. $m^{-2}$). The conventional multi-variate statistics (cluster analysis and non-metric multi-dimensional scaling) applied to assess spatial variation in macrobenthic assemblages. Cluster analysis and nMDS ordination analysis based on the Bray-Curtis similarity identified 2 major station groups. The major group 1 was associated with sand dominated stations and was characterized by high abundance of the bivalves Mactra chinensis, Siliqua pulchella and the polychaete Protodorvillea egena. On the other hand, major group 2 was connected with mud dominated stations and was numerically dominated by the polychaetes M. californiensis, M. japonica, Sternaspis scutata, S. longifolia and the bivalves Thyasira tokunagai and Theora fragilis. However, macrobenthic community structure were no significant differences between the environmental variables (sediment type and depth) and heated discharge.

Effect of Fertilizer and Organic Matter Level on Marketable Tuber Production in Chinese Yam (Dioscorea opposita) (마(산약) 생산을 위한 시비법 개선연구)

  • Shin, Jong-Hee;Kim, Sang-Kuk;Kang, Dong-Kyoon;Park, Sang-Zo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.2
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    • pp.144-150
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    • 2014
  • Yams (Dioscorea spp), which are edible or medicinal tuber crops, are a important crop in South Korea. Yams require a high level of soil fertility. The various cultural practices such as fertilizing and plowing were tested for marketable tuber production in Chinese yam (Dioscorea opposita). Tuber yield was also affected by organic matters in soil. Application higher level of organic matters result in increased each tuber weight and tuber yield per unit area. The nutrient absorption quantity of the plant such as nitrogen, phosphoric acid, calcium and potassium was increased from 100~120 days after planting, which time to begin tuber enlargement. The tuber yield was increased when the fertilization increased in quantity. Total yield and marketable ratio were the highest in 31~32 kg/10a of nitrogen fertilizer. From above result, income become larger with increase of marketable yield and quality improvement at 63% (27 kg/10a) level of conventional N fertilization (43 kg/10a). The tuber yield was not significantly different between with in various application level of potash fertilizer. Tuber size and weight decreased accordingly to decreased fertilizing level, so the rates of small tubers increased greatly at cultivation without chemical fertilizer. In considering the accumulation rates of allantoin in Chinese yam tubers, the apt harvest season was after October. The allantoin quantity of it was not influenced with nitrogen fertilizing. Moreover it was advantageous with decrease of chemical fertilizer and appropriate fertilizing in soil environment protection. Commercial tuber's number and yield were increased in trenching before planting with trencher compared with rotavating with tractor.

An Observation Study on the Health Benefits of Fermented Milk in Relation to Gastrointestinal Diseases Prevention in Korean (유산균발효유(乳酸菌醱酵乳)의 음용(飮用)이 소화기질환(消化器疾患) 예방(豫防)에 미친 효과(效果)에 관한 조사연구(調査硏究))

  • Lee, Won-Chang;Yoon, Yoh-Chang
    • Journal of Dairy Science and Biotechnology
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    • v.14 no.1
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    • pp.57-69
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    • 1996
  • This study was desiged to carry out an observation study on the cognitive level of health benefitis of fermented milk and subsequntly, on prevention of gastrointestinal diseases for the consumers in Korea. Data used in this study were collected from two different source ; 1) 987 university students living in Seoul, were selected randomly and interviwed induvidual from May 25 to September 30 1994, to investigate the awareness status as a consomer of fermented milk, and 2) health benefits of fermented milk with respect to prevention gastric intestinal diseases such as typhoid fever, paratyphoid fever and bacillary dysentery in Korea with the raw data from the Yearbook of Health and Social Statistics by Ministry of Health and Social Affairs (1976${\sim}$1995) and Ministry of Agriculture, Forestry & Fisheries (1976${\sim}$1995), Republic of Korea. The results of this study were as follows : The effects of femented milk on health appeared to be attributed to taking care of an environment of the gastrointestinal tract regarding diarrhea, constipation and digestion, with showing 75.1% of male and 71.1% of female of university students in Seoul answered positively, The correlation coefficients of statistics between amounts of consumption per capita of fermented milk and incidence rates of gastrointestinal diseases during the period from 1975 to 1994 in the whole country was r = -0.308 (p<0.05). those of Seoul a major comsuming area of fermented milk in the country and Kangwon province, a minor consuming area were r = -0.704 (p<0.01) and r = +0.262 (n.s.), respectively. In conclusion, the results obtained in this study suggest that fermented milk exert its effect on gastrointestinal tract. Furthermore, the results warrant a futher study in the field of preventive medicine and health science with respect to efficacy of fermented milk consumption.

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An Analysis of Work Stress of Physical Therapist and Reaction (물리치료사의 업무 스트레스 현황과 대응수준 -부산지역을 중심으로-)

  • Dong, Jong Ick;Ryu, Hwang Gun;Bae, Sung-Kwon
    • The Korean Journal of Health Service Management
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    • v.2 no.1
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    • pp.37-55
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    • 2008
  • This study aims to enhance work efficiency and satisfaction by offering data that make a physical therapist cope with stress coming from the job efficiently by identifying and analyzing the job satisfaction and work stress perceived by a physical therapist, and the level of reaction of a physical therapist. The study distributed survey questionnaires to 300 physical therapists working in medical institutions in Busan via mail and by visit from March 3, 2007 by selecting them simply and randomly from the physical therapist list, and collected the questionnaires by March 20, 2007, 103 respondents were working at 17 general hospitals including a university hospital, 65 respondents at 12 medical centers, and 79 respondents at 39 doctor's offices. The study collected 251 copies, which showed the collection rate of 83.7%, and analyzed 247 copies (82.3%) excluding 4 copies of insincere answers. As a research tool for measuring job satisfaction the study used a tool employed for research into the job satisfaction of physical therapists who work at medical institutions in Gwangju, Jeonnam, and Jeonbuk by Kim Hee-Gwon(1992) and research regarding job satisfaction by Jeong Jeong-Hee(2004) as well as research regarding the factors of job satisfaction by Flippo(1980) & Seberhagen(1970) after adjusting the research tools to the purpose of the study. Also for questions about work stress, the study employed nurses' job stress measurement tool developed by Kim Mae-Ja and em Mi-Ok(1984) by modifying the tool to the purpose of the study, and for a measurement tool for reaction to stress, the study used a tool employed for research into reaction to stress of nurses at general hospitals by Choi Eun-Deok(2005) without modification. For data analysis, the study used the SPSS12.0 as a statistical method, and then used t-test or ANOVA for verifying actual numbers, percentile, average :score, standard deviation, rank, and difference. Also, the study conducted which is a post-test method for variables that show a significant difference at the level of p<.05 level after the analysis. The findings include the following. 1) The respondents' job satisfaction score was 3.21 points on the average (out of 5 full points). The peer relationship ranked the highest, posting 4.02 points on the average, and the job satisfaction with rewards was proven the lowest, posting 2.51 points. For the job satisfaction level by characteristics, there were significant differences (p<.05) in gender, hospital type, weekly working hours, monthly working days, number of patients per day, department in charge of therapy, and number of peers, and there was no significant difference in characteristics other than that. 2) The respondents' work stress score was 2.72 points (out of 5 full points) on the average. The respondents were shown to be under the highest stress when they suffered from excessive workload, posting 3.49 points on the average, and they were shown to be under the least stress when they had a conflict with peers at another department, recording 1.90 points on the average. for the job stress level by the characteristics of job, there was a significant difference in the reflection of job assessment(p<.05). 3) 1n respondents' reaction to stress, most of them were shown to make efforts in coping with stress, posting 2.80 points (out of 5 full points). For their experience of being wider stress, they answered that 'they felt depressed (2.85 points)" for their experience of coping with stress, they answered that 'they were indifferent to it or thought about something else' (2.62 points). Also, for their efforts in coping with stress, they answered that 'they were motivated to remove their strain by taking leave, playing, or using their preferences' (3.52 points), which ranked higher. For the level of reaction to stress by characteristics, there were significant differences by age, gender, marital status, total service years as a physical therapist, monthly working days, and department in charge of therapy(p<.05). It is necessary to offer correct information by conducting an in-depth analysis of the stressful situations of physical therapists who exert efforts in rehabilitating patients at hospitals by factor, and seeking management plans based on the research results. Also, it is necessary to develop a program for coping with stress efficiently for removing stress and to conduct research into the understanding and cooperation of administrators and persons in charge of physical therapists for reducing physical therapists' stress at hospitals.

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Production and Characterization of Extracellular Polysaccharide Produced by Pseudomonas sp. GP32 (Pseudomonas sp. GP32에 의해 생산된 세포 외 다당류의 생산 및 특성)

  • Lee, Myoung Eun;Lee, Hyun Don;Suh, Hyun-Hyo
    • Journal of Life Science
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    • v.25 no.9
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    • pp.1027-1035
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    • 2015
  • A strain GP32 which produces a highly viscous extracellular polysaccharide was conducted with soil samples and identified as Pseudomonas species. The culture flask conditions for the production of extracellular polysaccharide by Pseudomonas sp. GP32 were investigated. The most suitable carbon and nitrogen source for extracellular polysaccharide production were galactose and (NH4)2SO4. The optimum carbon/nitrogen ratio for the production of extracellular polysaccharide was around 50. The optimum pH and temperature for extracellular polysaccharide production was 7.5 and 32℃, respectively. In batch fermentation using a jar fermentor, the highest extracellular polysaccharide content (15.7 g/l) was obtained after 70 hr of cultivation. The extracellular polysaccharide produced by Pseudomonas sp. GP32 (designated Biopol32) was purified by ethanol precipitation, cetylpyridinium chloride (CPC) precipitation, and gel permeation chromatography. Biopol32, which has an estimated molecular weight of over 3×107 datons, is a novel polysaccharide derived from sugar components consisting of galactose, glucose, gulcouronic acid and galactouronic acid in an approximate molar ratio of 1.85 : 3.24 : 1.00 : 1.42. The solution of Biopol32 showed non-Newtonian characteristics. The viscosity of Biopol32 exhibited appeared to be higher at all concentration compared to that of zooglan from Zoogloea ramigera. An analysis of the flocculating efficiency of Biopol32 in industry wastewater (food, textile, and paper wastewater) revealed chemical oxygen demand (COD) reduction rates 58.4-67.3% and suspended solid (SS) removal rates 82.6-91.3%. Based on these results, Biopol32 is a possible candidate for industrial applications such as wastewater treatment.

Comparison of Growth Characteristics, Forage Productivity and Quality between Italian Ryegrass and Synthetics Derived from Interspecific and Intergeneric Hybrids and Ecotypes (이탈리안 라이그라스와 종.속간 잡종 및 생태형 유래 합성종간의 생육특성, 수량성 및 사료가치 비교)

  • Choi, G.J.;Lim, Y.W.;Lim, Y.C.;Sung, B.R.;Kim, M.J.;Kim, K.Y.;Park, G.J.;Kim, S.R.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.4
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    • pp.265-272
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    • 2002
  • Forage breeding laboratory of National Livestock Research Institute, R.D.A. has made interspecific hybrids of Lolium multiflorum $\times$ L. pratensis and intergeneric hybrids of Lolium $\times$ Festuca since 1984, and has collected ecotypes of Italian ryegrass since 1991. Growth characteristics of these hybrids and ecotypes were researched, and then these clone lines were named. Among these clone lines, the several clones that have polen fertility, high cold-tolerance, and similar heading time were used for making synthetics, Naehan 6, 7, 8, 9, with polycrossing method in 1997. Field experiments were carried out to compare the mophological and agronomical characteristics and forage productivity and quality of the synthetics with those of Italian ryegrass varieties, Barmultra and Hwasan 101. in Suwon and Yonchun from 1999 to 2000. Heading time of the synthetics were 22th to 24th May that belong to late-mature types to be similar to that of Barmultra and Hwasan 101 in Suwon. The synthetics were 101 to 106 c3n in plant length, medium or thick in thickness of stem, dark peen in leaf color, broad and long in flag leaf, strong in lodging resistance, and excellent in regrowth. Winter survivals of the synthetics were no different from that of Barmultra or Hwasan 101 in Suwon, but better than that of Barmultra or Hwasan 101 in Yonchun where was -10 to -12$^{\circ}C$ of minimum average air temperature in January or February. Dry matter(DM) yields of the synthetics were similar to DM 8,238kg per ha of Barmultra in Suwon, but in Yonchun, were more 7 to 13% than DM 7,291kg per ha of Barmultra. Forage qualities, IVDMD, ADF, NDF and TDN of the synthetics were lower than those of Hwasan 101, but higher than those of Barmultra.

Assays of Maturity and Antifungal Activity against Plant Pathogen during the Animal Manure Composting Process (가축분 퇴비화 과정에서 부숙도 및 퇴비의 항균활성 검정)

  • Seo, Myung-Chul;So, Kyu-Ho;Park, Won-Mok
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.3
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    • pp.285-294
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    • 1999
  • Changes of chemico-physical properties and mturitiy during pig manure composting were analysed using three kinds of bulking agents with rice hull(T1), rice hull and extruding hull mixture (T2, 1:1, v/v), and extruding hull(T3). During composting process, temperature of T1, T2 and T3 were maintained over $50^{\circ}C$ for 31, 21, and 35 days respectively. Organic matter content of each treatment was decreased from 82.2%, 82.0%, and 82.8% to 70.5%, 68.9% and 69.7% and pH increased to 8.85, 9.91, and 8.80, respectively. Total nitrogen content of all treatments gradually decreased, but C/N ratio, phosphorous, and potassium content did not, show any changes during composting process. Both germination rate and early growth were tested using radish seeds for composting maturity. From those results, it was concluded that all treatments were stabilized after 45th day and extruding hull(T3) added compost was superior to others. The test of suppressive effect showed that all treatment have no effect against Fusarium oxysporum, Alternaria altemata, Botrytis cinerea. Compost supplemented with rice hull showed an inhibitory effect after 30th days, while compost supplemented with rice hull and extruding hull(T2) had an inhibitory effect during all period against Rhizoctonia solani. But treatment with extruding hull(T3) added compost did not have any inhibitory effect against Rhizoctonia solani. Only 63th samples in T1 and T2 treatment showed inhibitory effect against Colletoerichum gloeosporioides. However, T3 did not. Suppressive effect of extracts from 67 kinds of composts was investigated in vitro against plant pathogens, such as Fusauum oxysporum. Alternaria alternata, Colletotrichum gloeospoioides, Rhizoctonia solani, and Botrytis cinerea. Thirty two of them showed inhibitory effect against more than one phytopathogen, nine against one pathogen, four against two, six against three, six against four, and seven against five.

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Investigation on Characteristics of Swine Manure of Optimum Volume for Escalator Reversing Composting Facility (돼지분뇨 특성에 따른 기계교반 퇴비화시설의 적정용적 산정 연구)

  • Kwag, J.H.;Choi, D.Y.;Park, C.H.;Jeong, K.H.;Kim, J.H.;Yoo, Y.H.;Youn, C.K.;Ra, C.S.
    • Journal of Animal Environmental Science
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    • v.14 no.2
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    • pp.105-112
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    • 2008
  • This study was carried out to investigate evaporation rate of moisture per surface area and degradation rate of organic matter in full scale escalator reversing composting facility were analyzed to develope a computer program for the computation of an optimum volume of composting facility according to handling methods of swine farm, moisture levels of manure, degradation rate of organics and evaporation rate of moisture during composting. The obtained results can be followed as bellow; The temperature in full scale escalator reversing composting facility during composting reached $70^{\circ}C$ in 4 days and maintained until 11 days. Reduction rate of moisture and density was average 1.20% and 29.7%, respectively. Annual degradation rate of organic matter was 3.53%, showing lowest rate in winter as 3.23%. These seasonal degradation rate could be a factor to be considered for proper management and installation of composting facility. When computed with the amount of feces, urine, slurry and manure plus wastewater produced, the optimum volumes of composting facility for slurry and manure plus wastewater including each 95% moisture was $229m^3$ and $277m^3$, respectively, showing 21% ($48m^3$) difference.

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A Cohort Study on Risk Factors for Chronic Liver Disease: Analytic Strategies Excluding Potentially Incident Subjects (만성간질환 위험요인에 대한 코호트연구: 잠재적 발병자 집단을 감안한 분석전략)

  • Kim, Dae-Sung;Kim, Dong-Hyun;Bae, Jong-Myun;Shin, Myung-Hee;Ahn, Yoon-Ok;Lee, Moo-Song
    • Journal of Preventive Medicine and Public Health
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    • v.32 no.4
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    • pp.452-458
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    • 1999
  • Objectives: The authors conducted the study to evaluate bias when potentially diseased subjects were included in cohort members while analyzing risk factors of chronic liver diseases. Methods: Total of 14,529 subjects were followed up for the incidence of liver diseases from January 1993 to June 1997. We have used databases of insurance company with medical records, cancer registry, and death certificate data to identify 102 incident cases. The cohort members were classified into potentially diseased group(n=2,217) when they were HBsAg positive, serum GPT levels higher than 40 units, or had or has liver diseases in baseline surveys. Cox's model were used for potentially diseased group, other members, and total subjects, respectively. Results: The risk factors profiles were similar for total and potentially diseased subjects: HBsAg positivity, history of acute liver disease, and recent quittance of smoking or drinking increased the risk. while intake of pork and coffee decreased it. For the potentially diseased, obesity showed marginally significant protective effect. Analysis of subjects excluding the potentially diseased showed distinct profiles: obesity increased the risk, while quitting smoking or drinking had no association. For these intake of raw liver or processed fish or soybean paste stew increased risk; HBsAg positivity, higher levels of liver enzymes and history of acute liver diseases increased the risk. Conclusions: The results suggested the potential bias in risk ratio estimates when potentially diseased subjects were included in cohort study on chronic liver diseases, especially for lifestyles possibly modified after disease onset. The analytic strategy excluding potentially diseased subjects was considered appropriate for identifying risk factors for chronic liver diseases.

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