• Korean Journal of Ichthyology
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• v.29 no.3
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• pp.224-228
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• 2017

Two specimens of Ruvettus pretiosus (227.0 and 238.3 mm in standard length), belonging to the family Gempylidae, was firstly collected by using a gill net from the eastern coastal waters of Jejudo Island, Korea in November 2013. This species is characterized by having the following morphological traits: XV, 17~19+2 dorsal fin rays; II, 15~16+2 anal fin rays; belly keeled between pelvic fin and anus; body covered with cycloid scales, interspaced with rows of sharp bony tubercles. R. pretiosus can be easily distinguished from three Korean Snake mackerels (Thyrsitoides marleyi, Rexea prometheoides and Gempylus serpens) by having belly keeled between pelvic fin and anus, and cycloid scales covered the body and interspaced with rows of sharp bony tubercles. Based on morphological and molecular approaches, the specimens were identified as R. pretiosus. We propose new Korean names, "Gi-reum-chi-sok" and "Gi-reum-chi" for the genus and species, respectively.

• Journal of Food Hygiene and Safety
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• v.28 no.1
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• pp.50-55
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• 2013

Since 1 June 2012, it is prohibited to sell oilfish as a food material but there are still many illegal cases of selling oilfish as if it is tuna or grilled Patagonian toothfish. So it is absolutely crucial to construct the system to distinguish the real food material from oilfish. There are two sorts of oil fish called Ruvettus pretiosus and Lepidocybirium flavobrunneum involved in Percifomes order and Gempylidae class. 16S DNA gene region in mitochondria was selected to design the specific primers. For design species-specific primer, the theoretical experiment were performed for the sequences of R. pretiosus, L. flavobrunneum, Thunnus thynnus, Thunnus albacores, Makaira mitsukurii and Xiphias gladius, registered at the Gene bank from the National Centre for Biotechnology Information, using BioEdit 7.0.9.0. program. Through the analysis of the result from experiments, it was possible to design the 4 kinds of primers to distinguish R. pretiosus and L. flavobrunneum. As a comparison group, 3 kinds of tuna and 4 kinds of billfishes were selected and experimental verification was performed. As a result, for R. pretiosus and L. flavobrunneum, R.P-16S-006-F/R.P-16S-008-R and L.F-16S-004-F/L.F-16S-006-R primers were selected eventually and PCR condition was established. In addition, 178bp and 238bp of PCR products were confirmed from the established condition and non-specific band was not amplified among similar species. Therefore, the species-specific primers developed in this study would be very useful and used in various ways such as internet shopping mall and illegal distributions with fast and scientific results.