• Journal of Korean Society of Rural Planning
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• v.24 no.1
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• pp.67-74
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• 2018

The rural areas are large residential space with fewer people than urban areas. That is why they are vulnerable to social services such as health care and security. This research analyzed the vulnerability of emergency relief service in rural village through text mining and the weighting value have been calculated. Based on the calculated statistics data, the police facilities are the most important, While the fire fighting and hospital facilities are important as well. In addition, the distance from the emergency relief service facility to the rural village was confirmed by using Open API. By combining these results, The vulnerable areas of the rural villages and the emergency relief service facilities were calculated and classified into 5 levels. For rural areas, the 1st class will have 33 places, following by 1,179 in 2nd class, 199 in 3rd class, 17 in 4th class and 8 in 5th class. Hence in order to further supplement the vulnerable areas to emergency relief service in villages, geographical relocation and policy approach of emergency relief service facilities are necessary.

• Journal of Agricultural Extension & Community Development
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• v.23 no.3
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• pp.295-308
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• 2016

The purposes of this research are to not only investigate the time-based and regional characteristics in process of development of rural tourism in China, but also explain the significances of rural tourism in China by economic-political significance and social-cultural significance. The characteristics and significances investigated throughout this research are as follows. First, the development of rural tourism in China can be divided by three stages: the first stage(1980-1990), the second stage(1990-2000), the third stage (2000 -). Second, the rural tourism in China has been mainly developed around the metropolitan city such as Beijing, Chengdu. But, they showed different characteristics in process of development of rural tourism. Third, rural tourism in China has been used in solving san-nong(三農) problems which are the most urgent issues in contemporary China and reducing the gaps of living standard between urban and rural area. Forth, rural tourism in China has contributed in satisfying the needs of travel and leisure caused by the improvement of living standard of people due to China's rapid economic growth. Last, it is suggested in this research that the legal and institutional systems be prepared to guarantee the benefits of farmers.

• Journal of the Korean Institute of Rural Architecture
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• v.15 no.3
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• pp.25-32
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• 2013

This study aims to suggest the improvement strategy of law-system for rural landscape planning. The results are as follows. First, for the establishment of the rural and fishing village landscape planning, it is necessary to change the concept of the scene into Landscape to watch from Landscape to feel from now on. Second, it is necessary to establish a rural and fishing village landscape planning of the local unit to manage the rural and fishing village landscape systematically. In addition, it is necessary to cooperate with the Scenic Conservation Act in contents of the basic planning when we perform rural and fishing village development business. Third, it is necessary to make an extra landscape management planning to consider local landscape resources to develop a rural and fishing village. Finally, with an improvement direction of the plan establishment, the action to practice and management in the future is necessary. This method may do wider public information with establishing the management mechanism of the rural and fishing village landscape.

• Journal of Korean Society of Rural Planning
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• v.6 no.2 s.12
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• pp.82-93
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• 2000

The concept of sustainable development in rural area, which is focused on both preservation of nature and human's amenity, has been common paradigm. The purpose of this study is to suggest the development directions for the environmentally friendly rural house. The planning items for developing sustainable rural house were selected through literature survey, case study, and their adaptation evaluation. The establishment of principles and the evaluation of planning items were analyzed by a survey to experts. The results are as follows : 1) It is showed that sustainability evaluation of rural village is rated as above the moderate degree, but rural house is rated as 3.4(average score). Especially, there were lowly evaluated the utility, material, structure and inner environment in rural house. 2) It is revealed that 76.7% of planning items are located in both the upper left and upper right hand quadrant of the action grid, which is drawn by the importance-performance analysis(IPA). And any item is not found in the lower right hand quadrant(Possible Overkill). 3) Two basic principles and eight optional principles we identified by analysis of factor and regression. The rural zone is identified as major effective element for applying planning items into the rural house development. So it is desirable to apply the items differently by the rural zone type. 4) In view of sustainable development, it is desirable for rural village to have about 50 houses, and for rural house to have $155{\sim}180$ pyung(坪) as lot size, 30% as ratio of building, and 60% as building volume.

• Journal of Veterinary Science
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• v.22 no.4
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• pp.56.1-56.10
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• 2021

Background: Fluorescent antibody virus neutralization (FAVN) test is a standard assay for quantifying rabies virus-neutralizing antibody (VNA) in serum. However, a safer rabies virus (RABV) should be used in the FAVN assay. There is a need for a new method that is economical and time-saving by eliminating the immunostaining step. Objectives: We aimed to improve the traditional FAVN method by rescuing and characterizing a new recombinant RABV expressing green fluorescent protein (GFP). Methods: A new recombinant RABV expressing GFP designated as ERAGS-GFP was rescued using a reverse genetic system. Immuno-fluorescence assay, peroxidase-linked assay, electron microscopy and reverse transcription polymerase chain reaction were performed to confirm the recombinant ERAGS-GFP virus as a RABV expressing the GFP gene. The safety of ERAGS-GFP was evaluated in 4-week-old mice. The rabies VNA titers were measured and compared with conventional FAVN and FAVN-GFP tests using VERO cells. Results: The virus propagated in VERO cells was confirmed as RABV expressing GFP. The ERAGS-GFP showed the highest titer (10^8.0 TCID₅₀/mL) in VERO cells at 5 days post-inoculation, and GFP expression persisted until passage 30. The body weight of 4-week-old mice inoculated intracranially with ERAGS-GFP continued to increase and the survival rate was 100%. In 62 dog sera, the FAVN-GFP result was significantly correlated with that of conventional FAVN (r = 0.95). Conclusions: We constructed ERAGS-GFP, which could replace the challenge virus standard-11 strain used in FAVN test.

• Korean Journal of Veterinary Research
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• v.61 no.2
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• pp.13.1-13.8
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• 2021

Mammalian reovirus (MRV) causes respiratory and intestinal disease in mammals. Although MRV isolates have been reported to circulate in several animals, there are no reports on Korean MRV isolates from wildlife. We investigated the biological and molecular characteristics of Korean MRV isolates based on the nucleotide sequence of the segment 1 gene. In total, 144 swabs from wild animals were prepared for virus isolation. Based on virus isolation with specific cytopathic effects, indirect fluorescence assays, electron microscopy, and reverse transcription-polymerase chain reaction, only one isolate was confirmed to be MRV from a Korean roe deer (Capreolus pygargus). The isolate exhibited a hemagglutination activity level of 16 units with pig erythrocytes and had a maximum viral titer of 10^5.7 50% tissue culture infectious dose (TCID₅₀)/mL in Vero cells at 5 days after inoculation. The nucleotide and amino-acid sequences of the partial segment S1 of the MReo2045 isolate were determined and compared with those of other MRV strains. The MReo2045 isolate had nucleotide sequences similar to MRV-3 and was most similar (96.1%) to the T3/Bat/Germany/342/08 strain, which was isolated in Germany in 2008. The MReo2045 isolate will be useful as an antigen for sero-epidemiological studies and developing diagnostic tools.

• Korean Journal of Veterinary Research
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• v.61 no.2
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• pp.19.1-19.7
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• 2021

Feline panleukopenia virus (FPV) causes leukopenia and severe hemorrhagic diarrhea, killing 50% of naturally infected cats. Although intact FPV can serve as an antigen in the hemagglutination inhibition (HI) test, an accidental laboratory-mediated infection is concern. A non-infectious diagnostic reagent is required for the HI test. Here, we expressed the viral protein 2 (VP2) gene of the FPV strain currently prevalent in South Korea in a baculovirus expression system; VP2 protein was identified by an indirect immunofluorescence assay, electron microscopy (EM), Western blotting (WB), and a hemagglutination assay (HA). EM showed that the recombinant VP2 protein self-assembled to form virus-like particles. WB revealed that the recombinant VP2 was 65 kDa in size. The HA activity of the recombinant VP2 protein was very high at 1:2¹⁵. A total of 143 cat serum samples were tested using FPV (HI-FPV test) and the recombinant VP2 protein (HI-VP2 test) as HI antigens. The sensitivity, specificity, and accuracy of the HI-VP2 test were 99.3%, 88.9%, and 99.3%, respectively, compared to the HI-FPV test. The HI-VP2 and HI-FPV results correlated significantly (r = 0.978). Thus, recombinant VP2 can substitute for intact FPV as the serological diagnostic reagent of the HI test for FPV.

• Korean Journal of Veterinary Research
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• v.62 no.1
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• pp.10.1-10.9
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• 2022

Feline panleukopenia virus (FPV) causes fatal leukopenia and severe hemorrhagic diarrhea in cats. Although FPV isolates have been reported worldwide from several animals, the biological and genetic features of South Korean FPVs remain unclear. We characterized molecularly South Korean FPV isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate FPV from 60 organ homogenates. The isolates were confirmed to be FPVs via analyses of cytopathic effects, immunofluorescence studies, electron microscopy, and polymerase chain reaction. Viral genetic analyses used the full VP2 sequences. Eight isolates propagated in CRFK cells were confirmed to be FPVs. All isolates yielded viral titers ranging from 10^4.5 to 10^6.0 TCID₅₀/mL 5 days after inoculation into CRFK cells and exhibited hemagglutination titers ranging from 2⁷ to 2¹² (using pig erythrocytes). The Korean FPV isolates grew well in cat cells such as CRFK and Fcwf-4 cells. The FPV isolates were most similar to the KS42 strain isolated from a Korean cat in 2008. The FPV isolates will serve as useful antigens in future sero-epidemiological studies and will aid in the development of diagnostic tools.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.150-150
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• 2022

Plant reproduction associated with crop yields is highly vulnerable to global climate change components such as high and cold temperatures. The objectives of this study were to determine the effects of season-high exposure to temperature treatments in pollen morphology on Adzuki bean (V. angularis) and Mung bean (V. radiate). V. angularis and V. radiata were treated at high temperatures in the high temperature gradient greenhouse designed to cause temperature deviation. The pollen shapes treated at high temperature were compared by an electron scanning microscope. As a result, it was confirmed that the number of abnormal pollens morphology at high temperature was the least in V. radiata, and V. angularis was vulnerable to high temperatures. Also, it was found that the number of abnormal pollen morphology atT4 (Con +5~6℃) varied according to the cultivars of V. angularis. Therefore, the differences in Vigna species or cultivars with thermo-tolerance in pollen morphology to high temperature are projected to occur in the changeable future climate.

• Journal of Agricultural Extension & Community Development
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• v.13 no.1
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• pp.173-184
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• 2006

This study was diagnosed the managing of educational progress of homehelper training center proceeded with a goal to improve the proper direction to grasp of the contentment of education programs. In order to attain a research objective, I surveyed 18 charge of education person in homehelper training center. Through the problem of the mananging of homehelper education, I try to show the point for growth of homehelper training. First, the course of the education and training for home helper should be classified in the field of the service activity for home helper. Second, the education centers need to establish the weekend lecture or the night lecture for volunteer with an occupation. Third, the education centers need to division the educational course according a characteristic of student. Fourth, when the course is opened, the number of persons is suitable thirty. Fifth, it needs to add the education contents to establish an attitude with volunteer for elderly people.