• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.355-365
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• 2004

Two experiments were conducted to determine the effect of alkaline hydrogen peroxide (AHP) treatment (1% NaOH+1.5% $H_2O_2$; 1 AHPMS, 2% NaOH+1.5% $H_2O_2$; 2AHPMS) on rate and extent of degradation of mustard straw (MS) in sacco in sheep, and its in vivo digestion and ruminal fermentation characteristics when fed to sheep with concentrate (200 g per sheep daily). The treatment of straw with 1 and 2% AHP increased its sodium content by 148 and 296% to that of untreated straw (UMS). There was significant decrease in NDF and hemicellulose contents of AHP treated straw and increase in cellulose and lignin contents. Phenolic acids like ferrulic, $\rho$-coumaric and o-coumaric significantly (p<0.001) reduced by AHP treatment of mustard straw. In first experiment the in sacco degradation of DM, OM and NDF was significantly (p<0.01) greater for 2 AHPMS than for UMS at all incubation periods. The disappearance of nutrient from 1 AHPMS and 2 AHPMS treated straws continue to increase up to 96 h whereas in UMS the peak disappearance was found at 48 h. By using the equation {(y=a+b) ($1-e^{-ct}$)} the degradation rates (c) for DM, OM, and NDF were significantly higher for UMS than AHP treated straws. Level of alkali (1 and 2%) had significant effect on degradation characteristics (a, b, c and $P_{0.05}$) of DM and NDF fraction of MS. However, the effect was not pronounced on OM fraction of MS. In feeding experiment, the intake of nutrients for DM, OM, cell wall constituents and energy was higher on 2 AHPMS, whereas no effect on the digestibility of these nutrients was observed. The apparent nitrogen retention was higher (p<0.05) both in 1 and 2 AHPMS groups. Water intake by animals was significantly increased due to AHP treated mustard straw feeding. Rumen liquor pH was higher in 2 AHPMS fed animals. The $NH_3-N$ of rumen liquor was not affected by feeding of AHP treated MS based diets. Total VFA concentration was significantly (p<0.01) higher in UMS fed group. The fractional out flow rate of DM was higher (p<0.05) in animals fed on 2 AHPMS diets compared to UMS and lAHPMS fed groups. The population of large holotrichs was higher (p<0.05) on AHP treated MS fed diets compared to UMS. The study indicated that treatment of mustard straw with AHP changed its chemical composition towards a better feed. The nutritive value of 2% AHP treated mustard straw was better in terms of dry matter intake and apparent nitrogen retention. The higher in sacco DM, OM and NDF disappearance however, was not confirmed by in vivo data in this study.

• Journal of Animal Science and Technology
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• 제48권3호
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• pp.393-400
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• 2006

현미의 한우에 대한 사료가치 평가를 위해 옥수수 50%와 100%를 현미로 대체한 배합사료의 반추위 발효성상, in situ 영양소 분해율, in vivo 전장소화율을 측정한 바 결과는 다음과 같다. 옥수수와 현미의 체내 영양소 소화율과 영양가는 유사하였으나 반추위 내 분해율은 현미가 옥수수에 비하여 빠르게 나타났다. 옥수수와 현미가 함유된 시험사료를 급여한 후 반추위 pH는 시험사료 간 차이를 보이지 않았으나 NH3-N 농도는 현미 급여수준이 증가할수록 낮아졌다(P<0.05). 반추위액의 총휘발성지방산 함량은 옥수수구가 현미구에 비하여 높게 나타났으며, 초산과 프로피온산 비율(A/P)은 옥수수구에 비해 현미를 급여한 구에서 높은 수치를 보였다(P<0.05).이러한 결과를 볼 때 소 사료에 옥수수를 대체하여 현미를 사용하는 것에 영양학적으로 문제가 되지 않으며, 특히 곡류사료 위주 사양체계 하에서 낮은 초산과 프로피온산 비율에 대한 현미의 긍정적인 효과가 기대되어 이러한 곡류 내 가용성탄수화물의 이용성에 대한 다양한 연구가 수행되어야 할 것으로 사료된다.

• 한국토양비료학회지
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• 제45권6호
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• pp.1086-1093
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• 2012

본 연구는 유기성 바이오매스의 혐기소화율 평가에 주로 이용되는 VDI4630법에 대하여 소화액에 녹아 있는 탄산이온 ($CO_3{^{2-}}$)과 혐기소화 미생물 반응에 참여하는 수분 ($H_2O$)이 유기물의 혐기적 분해율에 미치는 영향을 분석하였으며, 이를 위해 탄산이온과 수분반응물에 의한 유기물의 혐기적 분해율 산출 보정식을 개발 하고자하였다. 돼지 혈액, 돼지 내장잔재물, 돼지 장내잔재물, 소 반추위잔재물의 화학조성식은 각각 $C_{3.78}H_{8.39}O_{1.46}N_1S_{0.01}$, $C_{9.69}H_{15.42}O_{2.85}N_1S_{0.03}$, $C_{25.17}H_{43.32}O_{15.04}N_1$, $C_{27.23}H_{42.38}O_{15.93}N_1S_{0.11}$으로 나타났으며, 돼지 혈액, 돼지 내장 잔재물, 돼지 장내잔재물, 소 반추위잔재물에서 이론적으로 1 mol의 유기물이 분해되는데, 0.336, 0.485, 0.227, 0.266 mol의 수분이 참여하였다. 혐기적 유기물 분해율에서 이론적 메탄생산퍼텐셜 대비 실험적 메탄생산퍼텐셜 ($B_u/B_{th}$)의 비율로 산출한 유기물 분해율은 돼지 혈액, 돼지 내장 잔재물, 돼지 장내잔재물, 소 반추위잔재물에서 각각 82.3, 81.5, 70.8, 66.1%이었으며, VDI4630에 근거한 유기물 분해율 (AB)은 각각 72.2, 87.8, 74.2, 62.0%를 보여 이론적 메탄생산퍼텐셜 대비 실험적 메탄생산퍼텐셜 ($B_u/B_{th}$)의 비율로 산출하는 유기물 분해율과는 전체 시험구에서 통계적으로 유의성 있는 차이를 보였다. VDI4630법에 소화액 중의 알칼리도를 보정한 유기물 분해율 (AB-I)은 돼지 혈액, 돼지 내장 잔재물, 돼지 장내잔재물, 소 반추위잔재물에서 각각 72.4, 88.1, 74.5, 62.1%를 보였으며, 알칼리도와 수분 반응물을 동시에 보정한 유기물 분해율 (AB-II)에서는 각각 72.5, 88.5, 74.5, 62.3%를 보여 본 연구에서 시험한 각각의 시료에서의 유기물 분해율 AB, AB-I, AB-II 간의 평균은 통계적으로 유의성 있는 차이를 보이지 않았다. 그러나 알칼리도, 수분 반응물의 보정식은 유기물의 혐기적 분해율의 측정에서 좀 더 높은 정확도를 보일 수 있을 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제9권2호
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• pp.181-187
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• 1996

About 8 million tons of straw (dry matter) become rotten during the monsoon(June to August) season in Bangladesh. The possibility of preserving straw with urea, under wet condition during dry period (December to May) and its utilization by cattle has been studied. Five tons of fresh and wet(600g DM/kg material) rice straw were preserved for six months, with 5% urea(W/W) in either dome or rectangular shaped heap without any polythene cover. The preservation quality, acceptability and nutritive value of preserved straw were then comparted with that of dry straw in growing cattle. In both types of heaps, straw was excellently preserved having strong ammonia smell,dark brown in colour with no fungal infestation. Urea preservation of straw increased its CP and ADF content. Preserved straw was readily accepted by the animals and they were healthy throughout the experimental period. Compared to dry straw, urea preserved straw had nonsignificantly higher rumen degradability, straw intgake and growth rate. Similarly, digestibilities of DM(p<0.01), OM(p<0.01) & ADF(p<0.01) were significantly higher in the preserved than the dry straw. It was concluded that wet straw with relatively lower moisture(400 to 500 g/kg straw) content can be readily preserved by using urea without being covered with polythene. Whether the same phenomenon occurs in the preservation of fresh and weet rice straw with relatively higher moisture(600 to 700 g/kg straw) content is yet to be determined.

• Asian-Australasian Journal of Animal Sciences
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• 제9권3호
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• pp.287-294
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• 1996

The objective of this study was to investigate the optimum level of the rumen protected lysine (RPLys) for early lactating Holstein dairy cow. This experiment was carried out with 16 Holstein dairy cows for 106 days and consisted of 4 treatments : $T_1$ (RPLys 0%), $T_2$ (RPLys 0.1%), $T_3$ (RPLys 0.2%) and $T_4$ (RPLys 0.3%). The results obtained are summarized as follows : 1. The daily intakes of feed were similar among treatments, but the digestibility of crude protein tended to increase 0.5-5.0% with increased level of RPLys and also the crude fiber digestibility increased (p < 0.05). 2. The daily weight gain for cows in $T_1$ was 253 g, which was lower than any other treatments (p < 0.05). The highest was 521 g in $T_3$. Also, the body condition score was changed from 3.22 at initial to 3.45 at final. The lowest increase in body condition score as 0.09 was obtained in control and the highest as 0.60 in $T_3$ (p < 0.01). 3. The total milk production of groups $T_2$, $T_3$ and $T_4$ were higher than $T_1$, as well as total protein, total fat and total solid yield. Especially in $T_4$ treatment group milk yield was higher than other treatments. The content of fat was higher in $T_2$ and $T_4$ compared to other treatments. Other components of milk were not significantly different (p > 0.05). The persistencies of lactation were increased in all RPLys treatments, especially, rate of reduction in milk yield was lowest in $T_4$ (p < 0.05). 4. The total amino acid content in the plasma, as well as plasma lysine content showed no consistent trend with treatments.

• Asian-Australasian Journal of Animal Sciences
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• 제22권11호
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• pp.1521-1530
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• 2009

A metabolic study was conducted with four ruminally-cannulated lactating goats (Saanen, 29 weeks lactation, 65${\pm}$5 kg) in a 4${\times}$4 Latin square design with 4 dietary treatments. The goats were fed a basal mixed diet consisting of 80% concentrate and 20% chopped rye grass hay (DM basis, CON). The goats were also fed the CON diet supplemented with soybean oil at a 5% level of the concentrate (SO), the SO diet supplemented with 0.5% of sodium bicarbonate (SO-B) or the SO-B diet supplemented with 30 ppm monensin (SO-BM). The goats were housed in individual pen and the study was conducted for 8 weeks. An increased molar proportion of propionate (C3) was observed at 1 h (p<0.003) and 6 h (p<0.029) post-feeding from all the supplemented diets. Calculated methane emission was markedly decreased prior to morning feeding (p<0.01), and at 1 h (p<0.05) and 6 h post-feeding (p<0.05) in goats fed the supplemented diets. All the supplements increased (p<0.0001) cis9, trans11-CLA content in rumen fluid. Concentrations of both cis9, trans11-CLA (p<0.0001) and trans10, cis12-CLA (p<0.026) were also increased in the milk fat of lactating goats fed the supplemented diets. The SO-B and SO-BM diets further increased CLA content in goat milk compared to the SO diet. All supplements increased unsaturated (UFA, p<0.002), monounsaturated (MUFA, p<0.002) and polyunsaturated fatty acids (p<0.014) and reduced SFA to UFA ratio (p<0.023). The concentration of MUFA was even greater (p<0.002) for SO-BM than for the SO-B diet. In conclusion, feeding soybean oil (5% of concentrate) to lactating goats was a useful way to improve milk fat and to improve fatty acid profile in the milk by increasing potentially healthy fatty acids such as CLA. Supplementation of sodium bicarbonate or sodium bicarbonate with monensin to the soybean oil-based diet increased CLA content further in goat milk. Supplementation of soybean oil may be an effective method to reduce methane emission in lactating goats.

• Asian-Australasian Journal of Animal Sciences
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• 제32권9호
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• pp.1381-1388
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• 2019

Objective: The aims of this study were to reveal the magnitude of the differences in protein structures at a cellular level as well as protein utilization and availability among soybean meal (SBM), canola meal (CM), and rapeseed meal (RSM) as feedstocks in China. Methods: Experiments were designed to compare the three different types of feedstocks in terms of: i) protein chemical profiles; ii) protein fractions partitioned according to Cornell Net Carbohydrate and Protein System; iii) protein molecular structures and protein second structures; iv) special protein compounds-amino acid (AA); v) total digestible protein and energy values; vi) in situ rumen protein degradability and intestinal digestibility. The protein second structures were measured using FT/IR molecular spectroscopy technique. A summary chemical approach in National Research Council (NRC) model was applied to analyze truly digestible protein. Results: The results showed significant differences in both protein nutritional profiles and protein structure parameters in terms of ${\alpha}-helix$, ${\beta}-sheet$ spectral intensity and their ratio, and amide I, amide II spectral intensity and their ratio among SBM, CM, and RSM. SBM had higher crude protein (CP) and AA content than CM and RSM. For dry matter (DM), SBM, and CM had a higher DM content compared with RSM (p<0.05), whereas no statistical significance was found between SBM and CM (p = 0.28). Effective degradability of CP and DM did not demonstrate significant differences among the three groups (p>0.05). Intestinal digestibility of rumen undegradable protein measured by three-step in vitro method showed that there was significant difference (p = 0.05) among SBM, CM, and RSM, which SBM was the highest and RSM was the lowest with CM in between. NRC modeling results showed that digestible CP content in SBM was significantly higher than that of CM and RSM (p<0.05). Conclusion: This study suggested that SBM and CM contained similar protein value and availability for dairy cattle, while RSM had the lowest protein quality and utilization.

• Asian-Australasian Journal of Animal Sciences
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• 제5권3호
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• pp.505-510
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• 1992

The experiments were carried out to study the effect of physical forms of hay diet on phosphorus metabolism by offering sheep roughage diets containing a low phosphorus content. The sheep were fed coarse hay, short hay or finely ground pelleted hay. The physical forms of hat diet had little effect on the pathway of phosphorus excretion. In all groups, the most of phosphorus was excreted in feces and urinary phosphorus excretion was negligible. Duodenal fluid flow, rumen fluid outflow and estimated salivary flow appeared to be relatively high in sheep fed the coarse hay diet as compared to those in sheep fed the finely ground diet. The amount of phosphorus flow to the duodenum tended to be high in the coarse diet group as compared to those in other two groups. On the other hand, phosphorus concentrations in the duodenal fluid and the rumen fluid were lower in sheep fed the coarse hay diet than those in sheep fed the ground hay. Net intestinal phosphorus absorption tended to be higher in sheep fed the coarse hay than that in sheep fed the ground hay or pelleted hay. However, a negative phosphorus balance was observed in the coarse hay diet group although a positive phosphorus balance was found in other two groups. It was considered that salivary phosphorus secretion was greater than the amount of salivary phosphorus reabsorbed from the intestine, resulting in the negative of phosphorus balance in sheep fed the coarse hay.

• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1690-1698
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• 2011

Feedlot and in vitro ruminal experiments were conducted to assess the effects of saponin-containing surfactant applied during tempering of barley grain on cattle growth performance and on ruminal fermentation. In the feedlot experiment, treatments with three barley grain/barley silage based diets were prepared using barley grain at 7.7% moisture (dry, D), after tempering to 18% moisture (M), or after tempering with a saponin-based surfactant included at 60 ml/t (MS). Each treatment was rolled at settings determined previously to yield optimally processed barley. A total of 180 newly weaned British${\times}$Charolais steers were fed three diets in 18 pens for a 63-d backgrounding period and 91-d finishing period to determine feed intake, growth rate and feed efficiency. Cattle were slaughtered at the end of the experiment to measure the carcass characteristics. Tempering reduced (p<0.001) volume weight and processing index, but processing characteristics were similar between MS and M. Tempering increased (p<0.05) growth during backgrounding only, compared with D, but did not affect feed intake in either phase. During backgrounding, feed efficiency was improved with tempering, but during finishing and overall this response was only observed with the surfactant. Tempering did not affect carcass weight, fat content or meat yield. Surfactant doubled the proportion of carcasses grading AAA. In the in vitro experiment, barley (500 mg; ground to <1.0 mm or steam-rolled) was incubated in buffered ruminal fluid (40 ml) without or with surfactant up to 20 ${\mu}l/g$ DM substrate for 24 h. Surfactant increased (p<0.05) apparent DM disappearance and starch digestibility but reduced productions of gas and the volatile fatty acid and acetate:propionate ratio, irrespective of barley particle size. Compared with feeding diets prepared with non-tempered barley, tempering with surfactant increased the feed efficiency of feedlot steers. This may have arisen from alteration in processing characteristics of barley grain by surfactant rather than its direct effect on rumen microbial fermentation.

• Asian-Australasian Journal of Animal Sciences
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• 제17권6호
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• pp.777-783
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• 2004

Samples of 13 species of forest tree leaves fed to livestock in the semi-hilly arid zone of Punjab State in India were collected at 30 d interval for 12 months, in order to assess their nutritional worth for livestock. The ground samples were pooled for 4 different seasons viz. dry hot, hot humid, fall and winter. The chemical composition irrespective of the season revealed that CP content varied between 8.9 (Carrisa) to 22.0% (Leucaena). Globulin was the major protein fraction in most of the leaves. The lowest concentration of cell wall constituents was observed in Morus alba and Grewea. The leaves in general became fiberous and lignified during winter and fall as compared to summer season. The leaves of Grewea, Morus alba, Leucaena, Carrisa and Acacia were rich in Ca, P and most of the trace elements. The total phenolics ranged between 1.88% (Azardirachta) to 15.82% (Acacia). The leaves of Acacia had the highest concentration of hydrolysable tannins (14.6%) whereas that of Carrisa had that of condensed tannins (5.9%). The condensed tannins (more than 3%) were negatively correlated to the digestibility of dry matter (DM), neutral detergent fiber (NDF) and crude protein (CP). The digestion kinetic parameters for DM, NDF and CP revealed that leaves of Morus alba, Zizyphus and Ehretia had highest insoluble but potentially degradable fraction. The minimum rumen fill values also revealed that leaves of Grewea, Azardirachta, Morus, Ehretia and Leucaena had great potential for voluntary DM intake. The leaves of Ougeinia, Malha, Dodenia and Carrisa had significantly higher rumen fill value indicating poor potential for voluntary DM intake. Season did not have any significant impact on digestion kinetic parameters except that most of the leaves had low potentially degradable fraction, which was degraded at slow rate during winter. It was concluded that the leaves of Morus, Ehretia, Grewea and Leucaena had great potential as livestock feed, while feeding of Ougeinia, Malha and Dodonea leaves should be avoided.