• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.607-614
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• 2005

To determine the optimum fermentation condition, the 50:50 feed mixture of both crushed corn and wet brewer’s grain were anaerobically fermented at various conditions. Three supplementation levels(0, 2%, and 5%) of molasses, five supplementation levels(0, 0.1%, 0.5%, 1.0%, and 2.0%) of yeast and five different incubation temperatures(4$^{\circ}C$, 10$^{\circ}C$, 30$^{\circ}C$, 40$^{\circ}C$ and 50$^{\circ}C$) were tested to determine the optimum fermentation conditions. During fermentation, alcohol concentration, live yeast cell number (LYCN) and pH values of the mixture were analyzed. Alcohol concentrations of the fermented feed mixtures were proportionally increased with increasing level of molasses supplementation. After 24hr fermentation, alcohol concentrations at 0.1%, 0.5%, 1.0% and 2.0% of yeast supplementation was 1.9%, 2.4%, 3.1% and 3.1%, respectively. These results indicate a proportional increase of alcohol concentration with the increasing supplementation of yeast (P<0.05) up to 1%, thereafter showing plateau at 2% supplementation. LYCN was relatively high with more than 0.5% yeast supplementation, whereas the value was significantly low(P<0.05) at 0.1% yeast supplementation. Alcohol production was significantly higher at 30$^{\circ}C$ and 40$^{\circ}C$ than other incubation temperatures (P<0.05), with the highest concentration at 30$^{\circ}C$. pH was rapidly decreased until 24 hours of incubation with steeper decrease at lower temperatures(P<0.05). Based on there results, optimum fermentation conditions for the alcoholic-fermented feed production could be achieved with 5% supplementation of molasses, 1% supplementation of yeast, and 24hr long incubation at 30$^{\circ}C$.

• Journal of Animal Science and Technology
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• v.47 no.5
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• pp.789-804
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• 2005

This study was conducted to determine effects of supplementation levels of aqueous direct-fed microbials (DFM; Bacillus spp.) to TMR(exp. 1.) and aqueous DFM addition under the various ratios of starch and cellulose(exp. 2.) on ruminal fermentation and fibrolytic enzyme activity. In experiment 1, ruminal fluids taken from rumen-cannulated Holstein cows were incubated during 24 hr by using TMR as substrates. Aqueous DFM was applied at a rate of 0, 0.025 and 0.05%, respectively. The pH of 0.025% treatment was not significantly different from that of control at 6 and 9 hr, but it was significantly lower (P<0.05) than 0.05% treatment. Concentrations of ammonia-N and VFAs were not affected by supplementing aqueous DFM. The A:P ratio of 0.05% treatment was significantly increased(P<0.05) by supplementation of aqueous DFM as compared with that of control at 24 hr. Although overall fibrolytic enzyme activities were not significantly affected by supplementing aqueous DFM, CMCase(carboxymethylcellulase) activity showed significant increase(P<0.05) compared to control at 6hr. However, the xylanase activity of 0.05% treatment significantly decreased(P<0.05) at 12 hr due to the application of aqueous DFM. There was no significant difference for in vitro dry matter disappearance among treatments. In experiment 2, ruminal fluids were incubated under the condition of various ratios of starch to cellulose(90:10, 70:30, 50:50, 30:70 and 10:90) with or without aqueous DFM(0.025%). Ruminal pH was unaffected by the addition of aqueous DFM, however, as increased level of starch, ruminal pH partially showed significant decrease(P<0.05). Ammonia-N concentration was not affected by aqueous DFM and ratio of starch and cellulose. On 9 hr incubation, DFM addition at a ratio of 70:30 showed significantly (P<0.05) lower value of ammonia-N(35.65 mg/dL) than that(65.05 mg/dL) of control. Concentrations of VFAs were significantly increased(P<0.05) by aqueous DFM addition compared with control at the same ratio on 6 hr incubation. The overall CMCase activity was not affected by aqueous DFM addition. However, the xylanase activity by aqueous DFM partially showed significant differences at the ratios of 90:10, 30:70 and 10:90. Our results indicated that supplementation of aqueous DFM did not significantly improve in vitro fermentation and fibrolytic enzyme activity. In addition, the DFM utilized in this study did not show consistent results by having various effects on ruminal fermentation under different feeding regimens.

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.219-226
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• 2006

The wastes (the needle leaves and branches) produced from thinning the forest were fermented under an anaerobic condition (pine silage) to utilize as a forage source of ruminants. An in situ trial was conducted with two ruminally fistulated Korean native cows by 4 replicates (2 bags per cow), and in vivo digestibility of pine silage was estimated with five Korean native steers by 5 replicates in incomplete double turn-over design. In order to investigate feed efficiency and carcass traits, forty eight Korean native bulls were assigned to four treatments (0%, 25%, 50%, 75% of pine silage) with a completely randomized design in 12 pens accommodating 4 animals per pen. The amounts of concentrate and roughage allowed to experimental animals were in the range of 2.5% and 0.6% of body weight, respectively. Animals had freely accessed to mineral block and water in stanchion barn.An in situ crude protein digestibility of the roughage sources when suspended for 48 hrs in the rumen was higher for feeding the pine silage than the rice straw, whereas NDF digestibility was vice versa. No differences between the treatments were observed in in vivo digestibilities of dry matter, crude protein and NDF. Daily body weight gain was significantly (P<0.01) higher for the pine silage substitutions compared with the rice straw feeding except for the 25% silage feeding. The pine silage feeding did not affect feed efficiency.In carcass traits, marbling score tended to be higher for the 50% pine silage treatment than the other treatments while back fat thickness tended to be the thinnest for the rice straw feeding. From these results, it may be recommended to substitute pine silage, as a forage source, for rice straw by 50 percent.