• Title/Summary/Keyword: Rubens

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Description of eight new mitochondrial genomes for the genus Neoarius and phylogenetic considerations for the family Ariidae (Siluriformes)

  • Luiz Guilherme Pereira Pimentel;Iuri Batista da Silva;Igor Henrique Rodrigues-Oliveira;Rubens Pasa;Fabiano Bezerra Menegidio;Karine Frehner Kavalco
    • Genomics & Informatics
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    • v.21 no.4
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    • pp.51.1-51.5
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    • 2023
  • The genus Neoarius, known as marine catfish, is a group of the family Ariidae, composed of 10 species found in Oceania. None of the species in this genus have their mitochondrial genome described, which is highly valuable in phylogenetic and molecular evolution studies. For the present work, eight species from the Neoarius genus were selected: Neoarius utarus, Neoarius midgleyi, Neoarius graeffei, Neoarius leptaspis, Neoarius berenyi, Neoarius paucus, Neoarius pectoralis, and Neoarius aff. graeffei. DNA sequences of the eight species were obtained through the NCBI Sequence Read Archive (SRA) database, and the mitochondrial genomes were assembled using the NOVOplasty tool on the Galaxy platform, subsequently annotated with the MitoAnnotator tool. We then utilized the protein-coding genes from the mitogenomes to estimate the phylogenetic relationships within the group, including seven additional mitogenomes available in the NCBI. In all species, the mitochondrial genomes presented 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 D-loop.

Selenium and Vitamin E for Prostate Cancer - Justifications for the SELECT Study

  • Ramamoorthy, Venkataraghavan;Rubens, Muni;Saxena, Anshul;Shehadeh, Nancy
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.7
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    • pp.2619-2627
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    • 2015
  • There are several studies that relate oxidative damage as possible mechanism for many cancers. Many studies have also shown that anti-oxidants like selenium and vitamin E decrease the risk for prostate cancer. The main objective of the Selenium and Vitamin E Cancer Prevention Trial (SELECT) study was to look for the benefits of selenium and vitamin E supplementation on prostate cancer. The study had a large sample size, stringent experimental conditions, very long duration, standardized laboratories for biochemical analyses and other factors that contribute to high external validity. The SELECT study failed to show any significant risk reduction for prostate cancers ascribable to selenium and vitamin E supplementations. Because of these conflicting results, many researchers argue about the methods used, supplementations administered (selenium and vitamin E) and indicators used for assessing levels of supplementations. We reviewed many epidemiological studies, clinical trials, and pre-clinical studies. With corroborative evidences we justify that SELECT study has a sound methodology and rationale. In lieu of the contrary results of the select study, researchers should focus on the probable mechanisms for these contrary findings and continue their search for newer and effective agents for prevention of prostate cancer.

Exploratory Study to Evaluate Changes in Serum Lipid Levels as Early Diagnostic and/or Prognostic Indicators for Oral Submucous Fibrosis and Cancer among Gutkha Consumers in India

  • Agarwal, Ankit;Saxena, Anshul;Rubens, Muni;Ahuja, Rinky;Tijare, Manisha
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.15
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    • pp.6439-6444
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    • 2015
  • Background: In India smokeless tobacco users account for 25.9% of total tobacco use, Gutkha being the most common form. This study evaluated the association between serum lipid profile and Gutkha use as an early diagnostic and/or prognostic indicator for oral submucous fibrosis (OSMF) and oral cancer (Oral Ca) in non-smokers and non-alcohol consumers. Materials and Methods: A total of 163 participants were recruited from two sites in India. Participants were divided into four groups: individuals without any Gutkha chewing habit and without any oral lesions (control group), individuals with Gutkha chewing habit but without any oral lesions (GWL), patients with a confirmed clinical diagnosis of OSMF, and patients with a confirmed diagnosis of Oral Ca. Mixed linear modelling (MLM) was conducted to detect the change in mean serum lipid levels among four groups. Results: The sample consisted of 69% males and 31% females. Results of MLM show an inverse relationship between serum lipid levels in OSMF, and Oral Ca groups when compared to the control group. Conclusions: The results of our study demonstrated that GWL, OSMF and Oral Ca patients had lower serum lipid levels. Low serum lipid levels could be an indicator of initial neoplastic changes in oral cancer.

Studies on Bionomics and Control of Cutworms (거세미나방류의 생태 및 방제에 관한 연구)

  • Kim H.S.;Kim S.H.;Choi K.M.
    • Korean journal of applied entomology
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    • v.19 no.4 s.45
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    • pp.243-248
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    • 1980
  • Experiments were conducted to study on bionomics and control of cutworms; Agrotis tokionis, A. ipsilon, A. fucosa in Suweon, 1978-1979. A. ipsilon and A. fucosa had two or three generations and A. tokionis had one generation a year. A large number of A. toikonis occured at the end of September, and the major peaks of the first generation of A. tokionis and A. fucosa were in mid-June, the second generation in mid-August, and the third generation was at the end of September. These cutworms laid many eggs on the lower surface of curved downward leaf of Chinese cabbage and the larvae later than 3rd instar began to cut the basal part of stem and then pulled into the soil. A parasite of A. tokionis, a braconid wasp, Meteorus rubens, and two unidentified Ichneumnid wasps were found. Mocap and Volaton gave effective control to the cutworms but Volaton should be applied not to contact with plant.

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Apical root canal cleaning after preparation with endodontic instruments: a randomized trial in vivo analysis

  • Fornari, Volmir Joao;Hartmann, Mateus Silveira Martins;Vanni, Jose Roberto;Rodriguez, Rubens;Langaro, Marina Canali;Pelepenko, Lauter Eston;Zaia, Alexandre Augusto
    • Restorative Dentistry and Endodontics
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    • v.45 no.3
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    • pp.38.1-38.10
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    • 2020
  • Objectives: This study aimed to evaluate vital pulp tissue removal from different endodontic instrumentation systems from root canal apical third in vivo. Materials and Methods: Thirty mandibular molars were selected and randomly divided into 2 test groups and one control group. Inclusion criteria were a positive response to cold sensibility test, curvature angle between 10 and 20 degrees, and curvature radius lower than 10 mm. Root canals prepared with Hero 642 system (size 45/0.02) (n = 10) and Reciproc R40 (size 40/0.06) (n = 10) and control (n = 10) without instrumentation. Canals were irrigated only with saline solution during root canal preparation. The apical third was evaluated considering the touched/untouched perimeter and area to evaluate the efficacy of root canal wall debridement. Statistical analysis used t-test for comparisons. Results: Untouched root canal at cross-section perimeter, the Hero 642 system showed 41.44% ± 5.62% and Reciproc R40 58.67% ± 12.39% without contact with instruments. Regarding the untouched area, Hero 642 system showed 22.78% ± 6.42% and Reciproc R40 34.35% ± 8.52%. Neither instrument achieved complete cross-sectional root canal debridement. Hero 642 system rotary taper 0.02 instruments achieved significant greater wall contact perimeter and area compared to reciprocate the Reciproc R40 taper 0.06 instrument. Conclusions: Hero 642 achieved higher wall contact perimeter and area but, regardless of instrument size and taper, vital pulp during in vivo instrumentation is not entirely removed.

Investigation of Enzymatic Activities in Marine Algae-Derived Fungi

  • Dawoon Chung;Woon-Jong Yu;Hyeong Seok Jang;Yong-Min Kwon;Seung Seob Bae;Grace Choi
    • Journal of Marine Life Science
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    • v.8 no.1
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    • pp.56-67
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    • 2023
  • Marine macroalgae are important in coastal ecosystems and interact with marine microorganisms. In this study, we isolated fungi from seven types of marine macroalgae including Cladophora sp., Gloiopeltis furcate, Gracilariopsis chorda, Hydroclathrus clathratus, Prionitis crispata, Sargassum micracanthum, and Ulva lactuca collected in Korea. Morphological and phylogenetic analyses identified the isolates as four Aspergillus spp. (A. fumigatus, A. sydowii, A. tamarii, and A. terreus), three Penicillium spp. (P. crustosum, P. jejuense, and P. rubens), and Cladosporium tenuissimum. Among them, A. fumigatus TOP-U2, A. tamarii SH-Sw5, and A. terreus GJ-Gf2 strains showed the activities of all enzymes examined (amylase, chitinase, lipase, and protease). Based on the enzymatic index (EI) values in solid media, A. terreus GJ-Gf2 and C. tenuissimum UL-Pr1 exhibited the highest amylase and lipase activities, respectively. Chitinolytic activity was only observed in A. terreus GJ-Gf2, A. tamarii SH-Sw5, and A. fumigatus TOP-U2. Penicillium crustosum UL-Cl2 and C. tenuissimum UL-Pr1 showed the highest protease activities. To the best of our knowledge, this is the first report of lipolytic and proteolytic activities in a marine-derived C. tenuissimum strain. Overall, the fungal strains isolated from the marine macroalgae in this study actively produced industrially important enzymes.

Morphometric Analyses with 15 Subspecies of Striped Field Mouse, Apodemus agrarius Pallas(Mammalia, Rodentia) from Eurasia (유라시아에서 서식하는 등줄쥐, Apodemus agrarius Pallas (포유 강,설치 목),15아종의 형태 형질의 분석)

  • Hung Sun Koh;G. Tikhonova
    • Animal Systematics, Evolution and Diversity
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    • v.14 no.4
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    • pp.341-355
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    • 1998
  • Thirty one morphometric characters of specimens in 15 subspecies of striped field mouse(Apodemus agrarius Pallas) from Eurasia were analyzed to clarify taxonomic status of these subspecies. Five major subgroups in A. agrarius were revealed: I, a largest-size form, specimens from two southern from other six localities in Korea, subspecies coreae and pallescens; III, the other large-size from, specimens from Astrachan in western Russia, subspecies volgensis; IV, a medium-size form, specimens from 16 localities in eastern Asia(North Korea, China, and eastern Russia), subspecies coreae, manchuricus, pallidior, ningpoensis, and insulaemus; V, a small-size form, specimens from 16 localities in western Asia and Europe (Kazakhstan, Russia, Lithuania, and Ukraine), subspecies tianschanicus, ognevi, agrarius, septentrionalis, nikolski, caucasicus, and karelicus. From this morphometric analyses, the followings are concluded: subspecies chejuensis is a larger-size form, as noted by Johnson and Jones(1955): subspecies pallescens is the synonym of subspecies agrarius, as suggested by Koh(1986): subspecies coreae from Korea is a large-size form and is idistinct from other 12 subspecies in Eurasia: the eastern form of subspecies ningpoensis by Corbet(1978) is a medium-size form o subspecies manchuricus, pallidior, ningpoensis, and insulaemus from eastern Asia (China and eastern Russia), and it includea North Korea specimens: a small-size form from western Asia and Europe(subspecies tianschanicus, ognevi, agrarius, septentrionalis, nikolski, caucasicus, and karelicus) is the western form of subspecies agrarius by Corbet(1978); the other large-size form of subspecies volgensis from western Russia is a distinct subspecies, which differs from the western subspecies agrarius. Therefore, it is concluded that 15 subspecies of A. agrarius can be classifed into five subspecies (chejuensis, coreae, ningpoensis, agrarius, and volgensis), although it is necessary to measure and analyze morphometric characters of specimens of other seven subspecies(albostriatus, maculatus, rubens, kahmanni, henrici, gloveri, and harti) for the complete reclassification of this species).

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Self-expandable metallic stent-induced esophagorespiratory fistulas in patients with advanced esophageal cancer

  • Iatagan R. Josino;Bruno C. Martins;Andressa A. Machado;Gustavo R. de A. Lima;Martin A. C. Cordero;Amanda A. M. Pombo;Rubens A. A. Sallum;Ulysses Ribeiro Jr;Todd H. Baron;Fauze Maluf-Filho
    • Clinical Endoscopy
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    • v.56 no.6
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    • pp.761-768
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    • 2023
  • Background/Aims: Self-expandable metallic stents (SEMSs) are widely adopted for the palliation of dysphagia in patients with malignant esophageal strictures. An important adverse event is the development of SEMS-induced esophagorespiratory fistulas (SEMS-ERFs). This study aimed to assess the risk factors related to the development of SEMS-ERF after SEMS placement in patients with esophageal cancer. Methods: This retrospective study was performed at the Instituto do Cancer do Estado de São Paulo. All patients with malignant esophageal strictures who underwent esophageal SEMS placement between 2009 and 2019 were included in the study. Results: Of the 335 patients, 37 (11.0%) developed SEMS-ERF, with a median time of 129 days after SEMS placement. Stent flare of 28 mm (hazard ratio [HR], 2.05; 95% confidence interval [CI], 1.15-5.51; p=0.02) and post-stent chemotherapy (HR, 2.0; 95% CI, 1.01-4.00; p=0.05) were associated with an increased risk of developing SEMS-ERF, while lower-third tumors were a protective factor (HR, 0.5; 95% CI, 0.26-0.85; p=0.01). No difference was observed in overall survival. Conclusions: The incidence of SEMS-ERFs was 11%, with a median time of 129 days after SEMS placement. Post-stent chemotherapy and a 28 mm stent flare were associated with a higher risk of SEMS-ERF.

Gross, organoleptic and histologic assessment of cadaveric equine heads preserved using chemical methods for veterinary surgical teaching

  • Rodrigo Romero Correa;Rubens Peres Mendes;Diego Darley Velasquez Pineros;Aymara Eduarda De Lima;Andre Luis do Valle De Zoppa;Luis Claudio Lopes Correia da Silva;Ricardo de Francisco Strefezzi;Silvio Henrique de Freitas
    • Journal of Veterinary Science
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    • v.25 no.2
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    • pp.29.1-29.11
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    • 2024
  • Background: Preservation of biological tissues has been used since ancient times. Regardless of the method employed, tissue preservation is thought to be a vital step in veterinary surgery teaching and learning. Objectives: This study was designed to determine the usability of chemically preserved cadaveric equine heads for surgical teaching in veterinary medicine. Methods: Six cadaveric equine heads were collected immediately after death or euthanasia and frozen until fixation. Fixation was achieved by using a hypertonic solution consisting of sodium chloride, sodium nitrite and sodium nitrate, and an alcoholic solution containing ethanol and glycerin. Chemically preserved specimens were stored at low temperatures (2℃ to 6℃) in a conventional refrigerator. The specimens were submitted to gross and organoleptic assessment right after fixative solution injection (D0) and within 10, 20, and 30 days of fixation (D10, D20, and D30, respectively). Samples of tissue from skin, tongue, oral vestibule, and masseter muscle were collected for histological evaluation at the same time points. Results: Physical and organoleptic assessments revealed excellent specimen quality (mean scores higher than 4 on a 5-point scale) in most cases. In some specimens, lower scores (3) were assigned to the range of mouth opening, particularly on D0 and D10. A reduced the range of mouth opening may be a limiting factor in teaching activities involving structures located in the oral cavity. Conclusions: The excellent physical, histologic, and organoleptic characteristics of the specimens in this sample support their usability in teaching within the time frame considered. Appropriate physical and organoleptic characteristics (color, texture, odor, and flexibility) of the specimens in this study support the use of the method described for preparation of reusable anatomical specimens.

Synesthetic Aesthetics in the Narrative, Painting and Music in the Film The Age of Innocence (영화 <순수의 시대>의 서사와 회화, 음악에 나타난 공감각적 미학 세계)

  • Shin, Sa-Bin
    • Journal of Popular Narrative
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    • v.27 no.1
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    • pp.265-299
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    • 2021
  • The purpose of this research paper is to facilitate the understanding of the synesthetic aesthetics in the film The Age of Innocence through the intertextuality among the narrative, paintings, and music in the film. In this paper, a two-dimensional intertextual analysis of the paintings in relation to the narrative is conducted on the paintings owned by Old New York, the paintings owned by Ellen, the portraits of unknown artists on the street outside of Parker House, and Rubens' painting at the Louvre. A three-dimensional intertextual analysis of performances in relation to the narrative is conducted on the stages and the box seats at the New York Academy of Music, in which Charles F. Gounod's Faust is performed, and the Wallack's Theatre, in which Dion Boucicault's The Shaughraun is performed. An intertextual analysis of music in relation to the narrative is also conducted on the diegetic and non-diegetic classical music of the film, including Beethoven's Piano Sonata No. 8 and Mendelssohn's String Quintet No. 2, as well as Elmer Bernstein's non-diegetic music of the film. The constituent event of The Age of Innocence represents the passion trapped in the reflection of love and desire that are not lasting, and the supplementary event embodies the narrow viewpoint and the inversion of values caused by the patriarchal authority of Old New York. The characters in the film live a double life, presenting an unaffected surface and concealing the problems behind it. The characters restrain their emotions at both the climax and the ending. The most powerful aspect of the film is the type and nature of oppressive life, which are more delicately described with the help of paintings and music, as there is a limit to describing them only by acting. In intertextual terms, paintings and music in The Age of Innocence continuously emphasize "feeling of emotions that cannot be expressed in language." With a synesthetic image, as if each part were imprinted on the previous part, the continuity "responds to continuous camera movements and montage effects." In The Age of Innocence, erotic dynamism brings dramatic excitement to the highest level, switching between the satisfaction of revealing desire and the disappointment of hiding desire due to its taboo status. This is possible because paintings and music related to the narrative have made aesthetic achievements that overcome the limitations of two-dimensional planes and limited frames. The significance of this study lies in that, since the identification in The Age of Innocence is based on the establishment of a synesthetic aesthetic through audio-visual representation of the film narrative, it helps us to rediscover the possibility of cinematic aesthetics.