• Title/Summary/Keyword: Rubber blade

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Oxygen Transfer in Animal Cell Culture by Using a Silicone Tube as an Oxygenator (실리콘 튜브를 이용한 동물세포 배양장치의 산소전달)

  • 정흥채;김정회
    • Microbiology and Biotechnology Letters
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    • v.20 no.4
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    • pp.445-450
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    • 1992
  • An enhancement of the oxygen transfer rate in a 1$\ell$ bioreactor for mammalian cell culture by using a silicone rubber tubing as an oxygenator was investigated. When the silicone membrane was used to supply oxygen to the culture broth, the oxygen transfer coefficients ($k_{\iota}a$) measured in deionized-distilled water were markedly increased. Effect of surface aeration without the tubing aeration was very low under $1.0hr^{-1}$ of $k_{\iota}a$. The enhancing effects of agitation rates on $k_{\iota}a$ were much more effective than those of aeration rates. The increase of $k_{\iota}a$ with increasing tube length was observed as a result of the large surface area for oxygen supply. However, 2 m of the tube length was adequate for a 1$\ell$ vessel. The larger blade type of impeller was effective to enhance the kLa values because of its high mixing intensity. In culture medium supplemented with 5% serum, kLa values were reduced to approximately 40% probably due to the viscosity. We also obtained the normal cell concentration of $5{\times}10^6$ cells/m$\ell$ of HepG2 on microcarriers, which could be achieved in a typical bioreactor for animal cell culture.

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Effects of Particle Size and Structure of Fillers on the Friction and Wear Behavior of Filled Elastomer (충전제의 입자크기 및 구조에 따른 고무 배합물의 마찰 마모특성)

  • Kaang, Shin-Young;Ryu, Chang-Seok;Hong, Chang-Kook;Moon, Chae-Woo
    • Elastomers and Composites
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    • v.41 no.3
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    • pp.194-204
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    • 2006
  • The effects of the particle size and the structure of carbon blacks on the friction and the wear behavior of filled natural rubber were investigated in this study. The particle size and the structure of carbon black had a significant effect on the wear rates and the worn surface pattern, and the effect of them on abrasion resistance should be considered for the optimum design of desired wear properties. Ten carbon blacks with various sizes and structures are mixed with natural rubber in order to investigate the effects on the wear rate ($W_R$). The friction and the wear behavior were examined by self-made blade type friction-wear abrader, and the ,elation with characteristic parameter (${\psi}=\sqrt{{N_2^2}+{DBP^2}}$), obtained from the particle size and the structure, was studied. The wear rate ($W_R$) had a Power Law relation with the frictional work ($W_f$) and it was inversely proportional to the characteristic parameter of carbon black. It means that smaller particle size and better structure development of carbon black resulted in improved abrasion resistance.

Automotive Windshield Wiper Linkage Dynamic Modeling for Vibration Analysis (자동차 와이퍼 링키지의 진동해석을 위한 동역학 모델링)

  • Lee, Byoung-Soo
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.18 no.4
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    • pp.465-472
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    • 2008
  • An automotive windshield wiper system is modeled mainly for vibration analysis purpose. The model is composed of solid links, ideal joints, imperfect joints to simulate unavoidable manufacturing defects and bushings having stiffness, contact between a wiper blade and a wind screen glass, friction, a spring and an actuator. Main stream of wiper dynamics analysis has been obtaining a closed form of system of equations using Newton's or Lagrange's formula and doing a numerical simulation study to understand and predict the behavior of it. However, the modeling process is complex since a wiper system is of multibody and a contact problem occurs. When imperfection, such as dead zone of a joint and stiffness of a rubber bushing, should be included, the added complexity makes the modeling difficult. Since the imperfection is understood as main cause of problematic vibration, the dynamics model of a wiper system aiming vibration analysis should include such unavoidable manufacturing defects in the model. An open form of dynamic model of a automotive windshield wiper system with imperfect joints using a commercial software is obtained and a simulation analyssis is conducted for vibration reduction study.

Study of Turbine Module Design for Die Casting Mold Release Injection Robot System (다이케스팅 이형재 분사 로봇시스템의 터빈 모듈 설계에 관한 연구)

  • Choi, Hyun-Jin;Son, Young-Bum;Park, Chul-Woo;Lee, Seung-Yong;Choi, Seong-Dae
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.14 no.5
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    • pp.1-7
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    • 2015
  • Cleaning by injecting dry ice and water is a generally adopted trend these days to clean molds (injection, diecasting foundry, press, rubber mold, etc). This cleaning method is performed manually, or by installing multiple high pressure spray nozzles. We have manufactured a turbine cleaning module device that is able to clean diecasting modules at any position and angle in the space by mounting an articulated robot instead of the existing pipe type injection nozzle, to minimize lead time and enhance working yield of the cleaning process. In this paper, we analyzed process factors that are required to design the turbine module by reviewing number of revolution, and results according to different blade angles and thicknesses of the mold release injection turbine module, using computational fiuid dynamics (CFD).

Comparative Analysis of Dietitians' and Cooks' Performance for Equipment Sanitation Management at School Foodservices in Gyeonggi Province (학교급식 기기류 소독관리에 관한 영양사와 조리사의 업무현황 비교분석 -경기도 지역-)

  • Lee, Mi-Jeong;Jang, Myeong-Suk;Lee, Jin-Mi
    • Journal of the Korean Dietetic Association
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    • v.13 no.3
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    • pp.250-264
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    • 2007
  • The purpose of this study was to compare dietitians' with cooks' actual sterilization performance for improving points of sanitation practices of cooking instruments and facilities in Kyonggi Province school foodservices. A total of 400 questionnaires were mailed to the dietitians and the cooks at elementary and middle schools in Kyonggi Province. Finally, 140 dietitians and 140 cooks replied by making a total of 280 replies(70%). The results of this study found that the cooks' attitudes toward the sterilization of the vegetable cutter body, multiple shelf, and distributing cart showed higher frequencies than the dietitians'. Cooks disinfected the vegetable cutter blade, multiple shelf, distributing cart, cook's aprons, and 'L' transporting cart better than dietitians' guides expected. In the sterilization method, cooks performed better than indicated when cooking with rubber gloves. The most difficult thing in dietitians' managing sterilization was the lack of cooking facilities and instruments. They strongly demanded the detailed explanatory notes according to their own Cities and Provinces and the circumstantial manual of disinfection parts in School Foodservice Sanitary Management Guide. There were possibilities of food-poisoning, infection and safety incidents due to the budgetary shortage of cooking facilities and instruments.

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Characteristics of Hybrid Protective Materials with CNT Sheet According to Binder Type

  • Jihyun Kwon;Euisang Yoo
    • Elastomers and Composites
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    • v.57 no.4
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    • pp.197-204
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    • 2022
  • Recently, the demand has increased for protective clothing materials capable of shielding the wearer from bullets, fragment bullets, knives, and swords. It is therefore necessary to develop light and soft protective clothing materials with excellent wearability and mobility. To this end, research is being conducted on hybrid design methods for various highly functional materials, such as carbon nanotube (CNT) sheets, which are well known for their low weight and excellent strength. In this study, a hybrid protective material using CNT sheets was developed and its performance was evaluated. The material design incorporated a bonding method that used a binder for interlayer combination between the CNT sheets. Four types of binders were selected according to their characteristics and impregnated within CNT sheets, followed by further combination with aramid fabric to produce the hybrid protective material. After applying the binder, the tensile strength increased significantly, especially with the phenoxy binder, which has rigid characteristics. However, as the molecular weight of the phenoxy binder increased, the adhesive force and strength decreased. On the other hand, when a 25% lightweight-design and high-molecular-weight phenoxy binder were applied, the backface signature (BFS) decreased by 6.2 mm. When the CNT sheet was placed in the middle of the aramid fabric, the BFS was the lowest. In a stab resistance test, the penetration depth was the largest when the CNT sheet was in the middle layer. As the binder was applied, the stab resistance improvement against the P1 blade was most effective.

APICAL FITNESS OF NON-STANDARDIZED GUTTA-PERCHA CONES IN SIMULATED ROOT CANALS PREPARED WITH ROTARY ROOT CANAL INSTRUMENTS (전동화일로 형성된 근관에서 비표준화 Gutta-percha Cone의 적합성)

  • Kwon, O-Sang;Kim, Sung-Kyo
    • Restorative Dentistry and Endodontics
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    • v.25 no.3
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    • pp.390-398
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    • 2000
  • The purpose of this study was to evaluate the apical fitness of non-standardized gutta-percha cones in root canals prepared with rotary Ni-Ti root canal instruments of various tapers and apical tip sizes. Simulated sixty curved root canals of plastic blocks were prepared with crown-down technique using rotary root canal instruments of Maillefer ProFile$^{(R)}$ .04 and .06 taper (Maillefer Instrument SA, Switzerland). Specimens were divided into six groups and prepared as follows: Group 1, prepared up to size 25 of .04 taper ; Group 2, prepared up to size 30 of .04 taper ; Group 3, prepared up to size 35 of .04 taper ; Group 4, prepared up to size 25 of .06 taper ; Group 5, prepared up to size 30 of .06 taper ; Group 6 ; prepared up to size 35 of .06 taper. After cutting off the coronal portion of plastic, blocks perpendicular to the long axis of the canal with the use of a diamond saw, apical 5mm of canal space was analyzed. Prepared apical canal spaces were duplicated using rubber base impression material to evaluate two dimensional total area of apical canal space. Various sized gutta-percha cones were applied in the 5mm-apical canal space, which were size 25, size 30 and size 35 standardized gutta-percha cone, Diadent Dia-Pro ISO-.04$^{TM}$ and .06$^{TM}$(Diadent, Korea), and medium-fine (MF), fine (F), fine-medium (FM) and medium (M) sized non-standardized gutta-percha cones (Diadent, Korea). Coronal excess gutta-percha were cut off with a sharp blade. Photographs of impressed apical canal spaces and gutta-percha cones were taken with a CCD camera under a stereomicroscope and stored in a computer. Areas of the total canal space and gutta-percha cones were calculated using a digitalized image analysing program, CompuScope (Sungjin Multimedia Co., Korea). Ratio of apical fitness was obtained by calculating the area of gutta-percha cone to the total area of the canal space. The data were analysed statistically using One-way Analysis of Variance and Duncan's Multiple Range Test. The results were as follows: 1. In canals prepared up to size 25 ProFile$^{(R)}$ of .04 taper, non-standardized MF and F cones occupied significantly more canal space than Dia-Pro ISO-.04$^{TM}$ or size 25 standardized ones (p<0.05). 2. In canals prepared up to size 30 ProFile$^{(R)}$ of .04 taper, non-standardized F cones occupied significantly more canal space than Dia-Pro ISO-.04$^{TM}$ or size 30 standardized ones (p<0.05), and non-standardized MF cones occupied more canal space than size 30 standardized ones (p<0.05). 3. In canals prepared up to size 35 ProFile$^{(R)}$ of .04 taper, there was no significant difference in canal space occupation among non-standardized MF and F, size 35 standardized, and Dia-Pro ISO-.04$^{TM}$ cones (p>0.05). 4. In canals prepared up to size 25 ProFile$^{(R)}$ of .06 taper, non-standardized MF and F cones occupied significantly more canal space than Dia-Pro ISO-.06$^{TM}$, or size 25 standardized ones (p<0.05), and Dia-Pro ISO-.06$^{TM}$, cones occupied significantly more space than size 25 standardized ones (p<0.05). 5. In canals prepared up to size 30 ProFile$^{(R)}$ of .06 taper, non-standardized FM cones occupied significantly more canal space than Dia-Pro ISO-.06$^{TM}$ or size 30 standardized ones (p<0.05), and non-standardized F cones occupied significantly more canal space than size 30 standardized ones (p<0.05). 6. In canals prepared up to size 35 ProFile$^{(R)}$ of .06 taper, non-standardized M and FM, Dia-Pro ISO-.06$^{TM}$ occupied significantly more canal space than size 35 standardized ones (p<0.05). In summary, in both canals prepared with .04 or .06 taper ProFile$^{(R)}$, non-standardized cones showed better fitness than Dia-Pro ISO$^{TM}$ or standardized ones, which was more characteristic in smaller canals.

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