• Asian-Australasian Journal of Animal Sciences
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• 제20권11호
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• pp.1705-1712
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• 2007

The effect of different tropical feed sources on rumen ecology, cellulolytic bacteria, feed intake and digestibility of beef cattle was investigated. Four fistulated, castrated male crossbred cattle were randomly allocated to a $4{\times}4$ Latin square design. The treatments were: T1) urea-treated (5%) rice straw (UTS); T2) cassava hay (CH); T3) fresh cassava foliage (FCF); T4) UTS:FCF (1:1 dry matter basis). Animals were fed concentrates at 0.3% of body weight on a DM basis and their respective diets on an ad libitum basis. The experimental period was 21 days. The results revealed that the use of UTS, CH, FCF and UTS:FCF as roughage sources could provide effective fiber and maintain an optimal range of ruminal pH and $NH_3-N$. Total viable and cellulolytic bacterial populations were enhanced (p<0.05) with UTS as the roughage source. Animals fed FCF had a higher rumen propionate production (p<0.05) with a lower cellulolytic bacteria count. Moreover, three predominant cellulolytic bacteria species, namely Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens, were found in all treatment groups. Roughage intake and total DM intake were highest with UTS (2.2 and 2.5% BW, respectively) as the roughage source (p<0.05). Nutrient intake in terms of organic matter intake (OMI) was similar in UTS, CH and UTS:FCF treatments (8.0, 6.8 and 8.7 kg/d, respectively), while crude protein intake (CPI) was enhanced in CH, FCF and UTS:FCF as compared to the UTS treatment (p<0.05). Digestion coefficients of DM and organic matter (OM) were similar among treatments, while the CP digestion coefficients were similar in CH, FCF and UTS:FCF treatments, but were higher (p<0.05) in CH than in UTS. CP and ADF digestible intakes (kg/d) were highest (p<0.05) on the CH and UTS treatments, respectively. It was also observed that feeding FCF as a full-feed resulted in ataxia as well as frequent urination; therefore, FCF should only be fed fresh as part of the feed or be fed wilted. Hence, combined use of FCF and UTS as well as CH and FCF were recommended.

• Journal of Animal Science and Technology
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• 제44권3호
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• pp.341-350
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• 2002

본 시험은 한우수소 24두를 이용하여 성장단계별 증체량 및 건물 섭취량을 측정하고, 그에 대한 추정식을 유도하기 위하여 수행되었다. 시험구는 조사료 3종류(볏짚, 건초, 옥수수사일리지)와 조사료 급여비율 4수준(100, 60, 40 및 20%)의 3${\times}$4요인시험으로서 공시축은 incomplete turnover design에 의해 처리당 4두씩 배치되었다. 조사료의 종류 및 급여비율에 따른 한우수소의 일일 증체량은 옥수수사일리지 급여구 및 조사료 20% 급여구에서 각각 가장 많았다. 한우수소에게 건초 혹은 옥수수사일리지만 전량 급여하였을 경우에도 전 기간동안 증체가 지속되었지만, 볏짚구에서는 체중 350kg대 이후부터 체중이 감소하였다. 대사체중당 건물 섭취량은 체중이 증가할 수록 줄어들었으며, 조사료 공급원과 조사료 급여비율에 따른 건물 섭취량은 옥수수사일리지 및 조사료 20%구에서 각각 가장 높았다. 대사체중당($BWkg^{0.75}$) 건물 섭취량(DMI, kg/ day)에 따른 한우수소의 일일 증체량(DG, kg) 추정공식은 다음과 같이 유도되었다. DG = －0.842 + 17.5DMI/$BWkg^{0.75}$체중(BW, kg)과 일일 증체량(DG, kg)을 고려한 사료의 NDF 함량(NDF, %)과 건물 섭취량(DMI, kg/day)과의 관계식은 다음과 같이 유도되었다 DMI = 4.125 + 0.013BW + 1.075DG － 0.045NDF

• Asian-Australasian Journal of Animal Sciences
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• 제17권6호
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• pp.794-798
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• 2004

An attempt was made to improve the efficiency of growth and lactation performance of dairy heifers subjected to a stair-step growth scheme using rice straw as the sole roughage source. Twenty-four young Holstein heifers were randomly assigned to either control or test group. The control diet met the National Research Council (NRC) requirement, with heifers calving at 24 to 26 mo of age. The test group was individually fed according to a schedule of 3, 2, 4, 2, 5 and 2 mo in which feed intake was alternately 20% below or 25% above the NRC requirements. Heifers on the stair-step growth pattern gained more body weight and consumed less dry matter (10.80 and 11.22%, respectively), resulting an increasing growth efficiency compared with the control. Body condition, first estrus, first conception, services per conception and calving difficulty (data not shown) were not affected. Milk yield of the test group was 8.5% higher than that of the control group. During the early lactation period, the milk yield was significantly higher in the stair-step group than in that of the control group (p<0.05). Milk composition was not affected by compensatory growth induced by the stair-step scheme. Also, weight at calving and calf growth performance was not affected by stair-step growth. The results indicate that using rice straw as a sole roughage source in a stair-step compensatory growth scheme can contribute to the improvement of growth efficiency and early lactation performance.

• 한국초지조사료학회지
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• 제32권4호
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• pp.387-396
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• 2012

A study was conducted to determine the dietary effects of Na-bentonite (NaB) and probiotics on meat characteristics and health of Hanwoo steers fed rice straw as a sole roughage source. A total of 24 growing Hanwoo steers (avg BW 232 kg) were assigned to two treatments which included a control diet (concentrate mix and rice straw) and a treatment diet (control diet + 0.5-1.0% NaB + 0.5-1.0% probiotics. The diets were fed for 22 months up to the time the animals were slaughtered. Dietary treatment increased (p<0.05) concentrations of trace minerals such as Zn, Cu, and Fe in the longissimus muscle compared to the control. The treatment diet did not affect cold carcass weight, yield traits such as backfat thickness, longissimus muscle area, yield index, yield grade and quality traits such as marbling score, meat color, fat color, texture, maturity and quality grade. Blood profiles of growing steers were within the normal ranges for healthy cattle. In conclusion, feeding a combination of clay mineral and probiotics to Hanwoo steers fed rice straw as a sole roughage source could have a desirable effect on improving trace mineral retention in longissimus muscle without any deleterious effects on carcass traits of steers.

• Asian-Australasian Journal of Animal Sciences
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• 제31권12호
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• pp.1890-1896
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• 2018

Objective: As the climate changes, it influences ruminant's feed intake, nutrient digestibility, rumen methane production and emission. This experiment aimed to evaluate the effect of feeding Sweet grass (Pennisetum purpureum cv. Mahasarakham; SG) as a new source of good quality forage to improve feed utilization efficiency and to mitigate rumen methane production and emission. Methods: Four, growing crossbred of Holstein Friesian heifers, 14 months old, were arranged in a $4{\times}4$ Latin square design to receive four dietary treatments. Treatment 1 (T1) was rice straw (RS) fed on ad libitum with 1.0% body weight (BW) of concentrate (C) supplementation (RS/1.0C). Treatment 2 (T2) and treatment 3 (T3) were SG, fed on ad libitum with 1.0% and 0.5% BW of concentrate supplementation, respectively (SG/1.0C and SG/0.5C, respectively). Treatment 4 (T4) was total Sweet grass fed on ad libitum basis with non-concentrate supplementation (TSG). Results: The results revealed that roughage and total feed intake were increased with SG when compared to RS (p<0.01) while TSG was like RS/1.0C treatment. Digestibility of nutrients, nutrients intake, total volatile fatty acids (VFAs), rumen microorganisms were the highest and CH4 was the lowest in the heifers that received SG/1.0C (p<0.01). Total dry matter (DM) feed intake, digestibility and intake of nutrients, total VFAs, $NH_3-N$, bacterial and fungal population of animals receiving SG/0.5C were higher than those fed on RS/1.0C. Reducing of concentrate supplementation with SG as a roughage source increased $NH_3-N$, acetic acid, and fungal populations, but it decreased propionic acid and protozoal populations (p<0.05). However, ruminal pH and blood urea nitrogen were not affected by the dietary treatments (p>0.05). Conclusion: As the results, SG could be a good forage to improve rumen fermentation, decrease methane production and reduced the level of concentrate supplementation for growing ruminants in the tropics especially under global climate change.

• Asian-Australasian Journal of Animal Sciences
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• 제27권1호
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• pp.36-45
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• 2014

The objective of this study was to determine the effects of protein sources and roughage (R) to concentrate (C) ratio on in vitro fermentation parameters using a gas production technique. The experimental design was a $2{\times}5$ factorial arrangement in a completely randomized design (CRD). Factor A was 2 levels of protein sources yeast fermented cassava chip protein (YEFECAP) and soybean meal (SBM) and factor B was 5 levels of roughage to concentrate (R:C) ratio at 80:20, 60:40, 40:60, 20:80, and 0:100, respectively. Rice straw was used as a roughage source. It was found that gas production from the insoluble fraction (b) of YEFECAP supplemented group was significantly higher (p<0.05) than those in SBM supplemented group. Moreover, the intercept value (a), gas production from the insoluble fraction (b), gas production rate constants for the insoluble fraction (c), potential extent of gas production (a+b) and cumulative gas production at 96 h were influenced (p<0.01) by R:C ratio. In addition, protein source had no effect (p>0.05) on ether in vitro digestibility of dry matter (IVDMD) and organic (IVOMD) while R:C ratio affected the IVDMD and IVOMD (p<0.01). Moreover, YEFECAP supplanted group showed a significantly increased (p<0.05) total VFA and $C_3$ while $C_2$, $C_2:C_3$ and $CH_4$ production were decreased when compared with SBM supplemented group. In addition, a decreasing R:C ratio had a significant effect (p<0.05) on increasing total VFA, $C_3$ and $NH_3$-N, but decreasing the $C_2$, $C_2:C_3$ and CH4 production (p<0.01). Furthermore, total bacteria, Fibrobacter succinogenes, Ruminococcus flavefaciens and Ruminococcus albus populations in YEFECAP supplemented group were significantly higher (p<0.05) than those in the SBM supplemented group while fungal zoospores, methanogens and protozoal population remained unchanged (p>0.05) as compared between the two sources of protein. Moreover, fungal zoospores and total bacteria population were significantly increased (p<0.01) while, F. succinogenes, R. flavefaciens, R. albus, methanogens and protozoal population were decreased (p<0.01) with decreasing R:C ratio. In conclusion, YEFECAP has a potential for use as a protein source for improving rumen fermentation efficiency in ruminants.

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1583-1591
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• 2013

The objective of this study was to investigate microbial population in the rumen of dairy steers as influenced by supplementing with dietary condensed tannins and saponins and different roughage to concentrate ratios. Four, rumen fistulated dairy steers (Bos indicus) were used in a $2{\times}2$ factorial arrangement in a $4{\times}4$ Latin square design. The main factors were two roughage to concentrate ratios (R:C, 60:40 and 40:60) and two supplementations of rain tree pod meal (RPM) (0 and 60 g/kg of total DM intake). Chopped 30 g/kg urea treated rice straw was used as a roughage source. All animals received feed according to respective R:C ratios at 25 g/kg body weight. The RPM contained crude tannins and saponins at 84 and 143 g/kg of DM, respectively. It was found that ruminal pH decreased while ruminal temperature increased by a higher concentrate ratio (R:C 40:60) (p<0.05). In contrast, total bacterial, Ruminococus albus and viable proteolytic bacteria were not affected by dietary supplementation. Numbers of fungi, cellulolytic bacteria, Fibrobactor succinogenes and Ruminococus flavefaciens were higher while amylolytic bacteria was lower when steers were fed at 400 g/kg of concentrate. The population of Fibrobactor succinogenes, was found to be higher with RPM supplementation. In addition, the use of real-time PCR technique indicated that the population of protozoa and methanogens were decreased (p<0.05) with supplementation of RPM and with an increasing concentrate ratio. Supplementation of RPM and feeding different concentrate ratios resulted in changing the rumen microbes especially, when the animals were fed at 600 g/kg of concentrate and supplemented with RPM which significantly reduced the protozoa and methanogens population.

• Journal of Animal Science and Technology
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• 제48권3호
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• pp.401-414
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• 2006

젖소 거세우의 육질을 개선시키기 위하여 조사료원으로서 대나무(잎과 가지포함)를 암모니아 처리 볏짚 급여수준에 대하여 4 수준(0, 30, 40, 50%)으로 나누어 체중 300kg 부터 700kg 까지 급여하면서, 증체량, 사료섭취량, 사료효율, 경제성, 소화율 및 도체특성에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 일당증체량은 대나무 30% 급여수준은 암모니아 볏짚 급여구와 차이가 없었으나 대나무 40%와 50% 급여구는 일당증체량이 감소하였다. 농후사료섭취량은 조사료중의 대나무 급여수준에 의하여 처리구간에 큰 차이가 없었다. 조사료 섭취량은 대나무 급여수준이 높아질수록 약간씩 증가하는 경향이었다. 총 사료섭취량은 대나무 급여수준 간에 차이가 없었다. 사료효율은 대나무 30% 급여수준은 암모니아 처리 볏짚 급여구와 차이가 없었으나 대나무 40% 및 50% 수준에서는 나빠졌다. 경제성에 미치는 영향을 보면 조수익에서는 대나무 30% 급여구가 암모나아 볏짚 급여구에 비하여 약 13% 높았으나, 대나무 40%와 50% 급여구는 조수익이 낮았다. 건물, 조지방, 조단백질, 가용무질소물 및 조회분 소화율은 대나무 30% 급여구와 암모니아 처리 볏짚 급여구간에 차이가 없었으나, 조섬유 소화율은 암모니아 처리 볏짚 급여구가 대나무 급여구 보다 높았고, 대나무 급여수준 간에는 차이가 없었다. 도체율과 등지방두께는 처리구간에 차이가 없었으나 배 최장근 단면적은 대나무의 급여수준이 증가할수록 점차적으로 좁혀졌다. 육량등급은 처리구간에 같은 B 등급이었다. 육색, 지방색, 조직감, 성숙도 및 근내지방도도 처리구간에 차이가 없었고, 육질등급은 처리구간에 2 등급이었다. 그러므로 본 연구에 의하면 젖소 수소는 거세를 시키고 조사료로서 암모니아 볏짚을 급여하면서 대나무(잎과 줄기포함)를 파쇄하여 30% 정도 대체 급여하면 젖소 거세우의 소득 향상을 위해 바람직하다고 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권2호
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• pp.198-205
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• 2011

This study was conducted to investigate the effect of feed types on Ochratoxin A (OTA) degradation by Korean native goats. Rumen fluid from canulated goats fed whole roughage or 50% roughage served as a source of micro-organisms. Experiments were undertaken i) to investigate OTA degradation ability in a $2{\times}4$ factorial arrangement with different feed types (100% roughage vs. 50% roughage) and rumen fluid fractions (whole rumen fluid, cells, autoclaved rumen fluid and supernatant) supplemented with OTA ii) to evaluate OTA degradation by the rumen fluid of goats fed two different diets at different time points (0, 3, 6, 9 and 12 h) of feeding iii) to isolate potential rumen microorganisms and iv) to identify elements responsible for OTA degradation. Rumen fluid from goats fed 100% roughage had higher (p<0.05) OTA degradability than 50% roughage diets. OTA degradation based on rumen fluid collection times showed that rumen fluid at 0 h showed significantly higher (p<0.05) degradability. Carboxypeptidase A (CPA) enzyme has been reported to be responsible for OTA degradation. Thus, using real time PCR, primers designed to target the CPA gene from Bacillus licheniformis could be amplified using genomic DNA from rumen fluid of goats and sequenced, thus enabling evaluation of the Bacillus population under different feeding condition and times. Our findings showed that the Bacillus population was significantly higher (p<0.05) before feeding (0 h) in animals which were fed a whole roughage diet, giving indirect evidence of OTA degradation being influenced by Bacillus sps. Thus, it can be concluded that OTA degradability is influenced by feed, feeding time and Bacillus licheniformis population.

• Asian-Australasian Journal of Animal Sciences
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• 제6권4호
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• pp.509-514
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• 1993

The experiment was conducted to determine the effect of different fiber levels on milk production of crossbred Holstein milking cows fed urea-treated rice straw (UTS) as a roughage. Eight cows were allotted into 2 squares of 4 cows each with 4 treatments by a balanced design. The treatments were 17%, 22%, and 24% crude fiber (CF) diets and Thai feeding system (free choice of roughage and 1 kg of concentrates/2 kg of milk) as a control. Body weight change was not significantly different among the treatments during the experiment. Milk production (4% FCM) and milk protein content wee not different among the treatments, but milk fat content was low in the 17% CF group and high in the control group. Cows fed the 17% CF diet consumed less UTS and more concentrates than the others, and consequently total DM intake was not different among the treatments. The feed conversion ratio was significantly higher in the control. Feed cost per kg milk was lowest in the control and highest in the 17% CF diet. The fiber content of the diet would be more than 17%, preferably 22-24% for normally producing Thai crossbred Holstein cows when the UTS was fed as a main roughage source.