• Title/Summary/Keyword: Rotation ratio

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Enhanced Chondrogenesis by Three-dimensional Co-culture of Chondrocytes and Mesenchymal Stem Cells (연골세포와 중간엽줄기세포의 3차원 Co-culture를 통한 연골화 향상)

  • Hwang, Seul-Gee;Cha, Hyun-Myoung;Lim, Jin-Hyuk;Lee, Ji-Hee;Shim, Hye-Eun;Kim, Dong-Il
    • KSBB Journal
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    • v.31 no.2
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    • pp.120-125
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    • 2016
  • Two-dimensional cultivation is typically used for cell growth, but the method reduces the characteristics of chondrocytes and stem cells, and limits culture area. Therefore, development of three-dimensional culture method is needed to mimic in vivo environment, improve quality of cells and scale-up efficiently. Improving proliferation and chondrogenesis is available by co-culture of chondrocytes and mesenchymal stem cells (MSCs) that leads to interaction between two kinds of cells. However, the co-culture has problems that permeability of sphere diminishes as aggregate size increased and ratio of two kinds of cells composing each spheres is different. In this work, co-cultivation method using controlled sphere composed of chondrocytes and MSCs was established and enhanced chondrogenesis. Periosteum-derived progenitor cells (PDPCs) that are appropriate for cell therapy source of articular cartilage were used as MSCs. Controlled spheres were formed in the hanging-drop plates and shifted for being induced chondrogenesis in 35-mm non-adhesive culture dishes at a rotation rate of 60 rpm. After inducing chondrogenesis, gene expressions related with chondrogenesis were found to be improved and it was apparent that the utilization of controlled spheres promoted chondrogenesis. As a result, available numbers of cells per unit area were increased and chondrogenic differentiation ability was improved compared to typical two-dimensional culture. This approach shows the potential in cartilage regeneration as it can provide sufficient numbers of chondrocytes.

Simplified Nonlinear Dynamic Progressive Collapse Analysis of Welded Steel Moment Frames Using Collapse Spectrum (붕괴스펙트럼을 활용한 용접철골모멘트골조의 비선형 동적 연쇄붕괴 근사해석)

  • Lee, Cheol Ho;Kim, Seon Woong;Lee, Kyung Koo;Han, Kyu Hong
    • Journal of Korean Society of Steel Construction
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    • v.21 no.3
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    • pp.267-275
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    • 2009
  • This paper presents the behavior of column-removed double-span beams in welded steel moment frames and proposes a simplified nonlinear dynamic analysis method for the preliminary evaluation of progressive collapse potential. The nonlinear finite element analysis and the associated analytical study showed that the column gravity load and the beam span-to-depth ratio govern the maximum dynamic deformation demand of the double-span beams. Based on these results, the concept of a collapse spectrum, which describes the relationship between the gravity load parameter and the maximum chord rotation of the double-span beams, was newly proposed. A procedure for the application of the collapse spectrum to multi-story welded steel moment frames was then suggested. The inelastic dynamic finite element analysis results showed that the proposed method gives satisfactory prediction of the nonlinear progressive collapse behavior of welded steel moment frames.

Torque Measurement of Rotating Shaft Using Fiber Bragg Grating Sensors and Rotary Optical Coupler (광섬유격자센서와 회전광학커플러를 사용한 새로운 회전축의 토크 측정방법)

  • Lee, Jong-Min;Hwang, Yo-Ha
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.17 no.12
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    • pp.1195-1200
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    • 2007
  • Torque of a rotating shaft has been mostly measured by strain gages combined with either a slip ring or telemetry. However, these methods have severe inherent problems like low S/N ratio, high cost, limited number of channels and difficult installation. In this paper, a new method using FBG(fiber bragg grating) sensors and a rotary optical coupler for online non-contact torque monitoring is suggested. FBG sensor can measure both strain and temperature, and has much batter characteristics than those of a strain gage. A rotary optical coupler is a optical connecting device between a rotating shaft and stationary side without any physical contact. It has been devised for transmitting light between a rotating optical fiber and a stationary optical fiber. The proposed method uses this rotary optical coupler to connect FBG sensors on the rotating shaft to instruments at stationary side. And a reference FBG sensor is also applied to compensate the insertion loss change of the rotary optical coupler due to rotation. Three FBG sensors have been fabricated in a single optical fiber. Two FBG sensors are attached on the shaft surface to measure torque and one sensor is installed at the shaft center to compensate the insertion loss change. The torque of a rotating shaft has been successfully measured by the suggested method proving its superior performance potential.

Lower Extremity Reconstruction by Perforator Based Flap (천공지 기저 피판을 이용한 다양한 하지 재건)

  • Oh, Chang Hyun;Shim, Jeong Su;Park, Dae Hwan
    • Archives of Plastic Surgery
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    • v.36 no.6
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    • pp.720-726
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    • 2009
  • Purpose: Fasciocutaneous flap with random pattern flap has limitation in mobility and length - width ratio. This characteristic is more pronounced in lower extremity which has relatively poor vascularity. Perforator based flap in lower extremity reconstruction has various advantages as a axial flap, allowing abundant blood supply and widening of mobility range. So if it is not a case of wide defect, free flap can be replaced by perforator based flap. Methods: From April 2007 to March 2009, 18 cases of perforator flap were performed. 8 had defect in upper 1/3 of calf, 6 in middle 1/3, and 4 in lower 1/3. In 10 cases island flap were used, 3 case had transposition flap, 2 cases used advancement flap, 2 case had propeller flap and 1 case had rotation flap. Results: 17 cases survived without flap necrosis. Partial flap necrosis occurred in 1 case, so secondary split thickness skin graft was done. Chronic wound with pseudomonas infection occurred in 1 case, but it was completely cured with conservative treatment. Conclusion: Perforator based flap is useful in lower extremity reconstruction because of relative freedom in changing the size and thickness of the flap depending on the recipient site, good mobility, and abundant vascularity. And donor site morbidity can be minimized. Lower extremity reconstruction using perforator based flap is a good method because it can minimize the complication and obtain effective result.

Removal of Ring Artifact in Computed Tomography (전산화단층촬영장치에서 링 아티팩트 제거)

  • Chon, Kwon Su
    • Journal of the Korean Society of Radiology
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    • v.9 no.6
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    • pp.403-408
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    • 2015
  • Hard X-ray has been widely used in medical and industrial fields because it can be applied to observe the inside of a sample. Computed tomography provides sectional images of the sample through the reconstruction of the projection images. The quality of sectional images strongly depends on that of projection images. Ring artifact appeared on the seconal image can be made by the abnormal pixels of the detector used. In this study, we examine the ring artifact ratio in the circle phantom as a function of detection error of the detector used in computed tomography. The ring artifact increased with the increment of detection error under parallel and fan beam geometries and strongly increased near the center of rotation. The corrections, dead pixel and flat field corrections, for the images taken with the detector are required before the image reconstruction process to reduce the ring artifact in the computed tomography.

Thickness Effect of ZnO Electron Transport Layers in Inverted Organic Solar Cells

  • Jang, Woong-Joo;Cho, Hyung-Koun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.08a
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    • pp.377-377
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    • 2011
  • Organic solar cells (OSCs) with low cost have been studied to apply on flexible substrate by solution process in low temperature [1]. In previous researches, conventional organic solar cell was composed of metal oxide anode, buffer layer such as PEDOT:PSS, photoactive layer, and metal cathode with low work function. In this structure, indium tin oxide (ITO) and Al was generally used as metal oxide anode and metal cathode, respectively. However, they showed poor reliability, because PEDOT:PSS was sensitive to moisture and air, and the low work function metal cathode was easily oxidized to air, resulting in decreased efficiency in half per day [2]. Inverted organic solar cells (IOSCs) using high work function metal and buffer layer replacing the PEDOT:PSS have focused as a solution in conventional organic solar cell. On the contrary to conventional OSCs, ZnO and TiO2 are required to be used as a buffer layer, since the ITO in IOSC is used as cathode to collect electrons and block holes. The ZnO is expected to be excellent electron transport layer (ETL), because the ZnO has the advantages of high electron mobility, stability in air, easy fabrication at room temperature, and UV absorption. In this study, the IOSCs based on poly [N-900-hepta-decanyl-2,7-carbazole-alt-5,5-(40,70-di-2-thienyl-20,10,30-benzothiadiazole)] (PCDTBT) : [6,6]-phenyl C71 butyric acid methyl ester (PC70BM) were fabricated with the ZnO electron-transport layer and MoO3 hole-transport layer. Thickness of the ZnO for electron-transport layer was controlled by rotation speed in spin-coating. The PCDTBT and PC70BM were mixed with a ratio of 1:2 as an active layer. As a result, the highest efficiency of 2.53% was achieved.

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An Analysis on the Constructional Factor of Slacks by Lower-Limb Movement (하지동작(下肢動作)에 따른 Slacks 구성요인(構成要因) 분석(分析))

  • Park, Young Deuk;Suh, Young Sook
    • Journal of the Korean Society of Clothing and Textiles
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    • v.17 no.4
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    • pp.648-662
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    • 1993
  • The summarized findings resulted from experiments and investigation are suggested as follows ; The analysis of measurements on the lower limb movements : For this experimentation, data was collected from three hundred and eighty female, age 19 to 23, who answered five lower limb movements(M1~M5). The statistics show that the order of the expansion ratio is gluteal area-length/knee-girth/back-line/knee-depth/thigh-depth/hip-girth, from the highest to the lowest in all movements. When comparing the correlation coefficient of the measurements, the values of the correlation coefficient of the height and the length items are very low, but those of the girth, the breadth, and the depth items are relatively high and those of the waist and the hip items are highest. For more sophisticated analysis, the factor analysis was conducted on the lower limb movements. Four factors were classified on the factor load by the "varimax rotation" method. Each movement shows the most important factor differently, as follows ; the most important factor in M1 is "the shape factor of lower limb below hip-line", that in M2 is "the cross-sectional shape factor", that in M3 is "the size factor of abdominal and loins region", and those in M4 and M5 accord with the interpretation of M3. When the investigation of the estimated function was conducted, in the selectional case of representative items on the slacks construction, it found that it would be better to add abdomen and thigh items as important considerations to waist girth, hip girth and crotch length.

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The Finite Element Analysis for a Micro Turbine Fabricated by LIGA-like Process (LIGA-like 공정으로 제작된 마이크로 터빈의 유한 요소 해석)

  • Oh, J.;Choi, B.;Kim, N.
    • Journal of Sensor Science and Technology
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    • v.9 no.5
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    • pp.380-388
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    • 2000
  • The finite element analysis of a micro turbine was made to investigate safety margin of its operating condition for the high aspect ratio nickel micro turbine blades fabricated by conventional LIGA-like method. From our study, we found that the fabricated turbine could not exceed its yield strength even if the pressure difference between inlet and outlet of turbine blade was about 44kPa, and the correlation of friction coefficient and the maximum stress, caused by contact friction between outer diameter of shaft and inner diameter of turbine blade, was somewhat reciprocal. The maximum stress was decreased with the increasing contact friction, when turbine blade was in its state of rotation. By the results of our study, we conclude that it is possible to fabricate metal micro turbine more easily han surface micromachining technology and to operate with no risk of metal structure's damage, which is caused by yield strength, if the operating condition with the design of micro turbine itself are optimized. It is useful to adopt other applications which have the contact problems between a moving part and the fixed one in micro structures.

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Finishing of Interior Fabric Using Soluble Micro-fiber and low melting Yarn (용출형 극세사와 저온 융착사를 이용한 인테리어 직물의 가공)

  • Ahn, Young-Moo
    • Journal of Fashion Business
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    • v.13 no.2
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    • pp.78-86
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    • 2009
  • When scouring and contraction finishing at $90^{\circ}C$ using Relaxer or Rotary Washer contraction and weight loss ratio in warp and weft directions were excellent. Also surface state of fabric after drying or sanding treatment was excellent without crease. Low melting polyester fabric showed a complete melting bond by heat setting(P/S) at above $160^{\circ}C$. The alkali hydrolysis reaction of polyester showed the breakpoint in the weight loss behavior test, polyester yarn showed a breakpoint ranging from 25% to 28%. This is due to the difference of the hydrolysis rate between regular polyester and soluble polyester. Initially the soluble polyester was eluted and micro-fibrillized 5 times faster than a regular polyester. At a later time, a regular polyester was reduced weight to impart a proper flexibility and drape property to the fabric. As a result of surface sanding finishing, the surface of interior fabric showed a surface state most stabilized when using Mesh No. 220 in mono 0.2d after elution finishing. When the rotation direction of sanding roller was pro-, pro-, pro-, and retro-direction, a directional effect of tuft was not shown, a writing effect as suede was exhibited and a surface state was even. Sublimation fastness was 3-4 class for polyester and 2-4 class for nylon. Light fastness 3-4 class after lapse of 100 hours and 2-4 class after lapse of 160 hours. Abrasion fastness was 3-4 class on wet and 4-5 class on dry Laundry fastness was 2-4 class. As such, the abrasion fastness is slightly reduced upon wetting and the use thereof for interior is excellent, whereas laundry fastness is slightly lowered.

Understanding of Blast Resistant Design and Performance Evaluation of a Building designed for Conventional Loads (방폭설계의 이해 및 일반하중에 대해 설계된 건축물의 방폭성능 평가)

  • Hong, Jong-Kook
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.22 no.4
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    • pp.83-90
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    • 2018
  • Considering the increased threats from worldwide terrors and the increased demands on the blast resistant design of commercial buildings, this study is aimed at understanding the basic concept of blast resistant design and evaluating the blast performance with an actual design example. Although there are many differences between earthquake and blast loads, the design concept against both loads is similar in terms of allowing the plastic behavior of a structure and sharing the ductile detailing. Through the blast performance evaluation of a target building provided in this study, it is noted that a well-designed building for the conventional loads can have a certain level of blast resistance. However, this cannot be generalized since the blast load on a structure varies depending on the type of weapon, TNT equivalence, standoff distance, etc. Architectural planning with positioning the sacrificial structure or maintaining a sufficient standoff distance from the expected detonation is the simple and effective way of improving the blast resistance of a building.