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Biodegradation of 4-t-Octylphenol by Basidioradulum molare and Schizopora paradoxa and Estrogenecity Reduction of its Metabolites (옥틸페놀(4-t-Octylphenol)의 Basidioradulum molare와 Schizopora paradoxa에 의한 분해 및 에스트로겐성 저감효과)

  • Lee, Soo-Min;Ku, Bon-Wook;Lee, Jae-Won;Choi, Don-Ha;Jeung, Eui-Bae;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.4
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    • pp.27-35
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    • 2004
  • Recalcitrant 4-t-Octylphenol used as a surfactant was subjected to the biodegradation with wood rot fungi, Basidioradulum molare and Schizopora paradoxa. Two fungi were grown in the culture medium containing various concentrations of 4-t-Octylphenol in order to investigate their resistance against 4-t-octylphenol Schizopora paradoxa was reached to the full growth within 14 incubation days in the concentration of more than 200 ppm of 4-t-Octylphenol, while Basidioradulum molare showed the inhibitory mycelium growth as the concentration was increased Schizopora paradoxa and Basidioradulum molare biodegraded 95% and 36% of initial concentration of 4-t-Octylphenol at first incubation day, respectively. However, the biodegradation capability reached to more than 95% after 3 incubation days. During the biodegradation of 4-t-Octylphenol, the activity of manganese dependent peroxidase was induced by the addition of 4-t-Octylphenol in the culture medium of Schizopora paradoxa, but that of laccase was maximal before the addition. The reduction of estrogenecity was assayed by MCF-7 cell proliferation test and measurement of pS2 mRNA expression. The level of pS2 mRNA was decreased down to the level of baseline at first incubation day. Also, estrogenecity of 4-t-Ocrylphenol completely disappeared after treatment with supernatant by Schizopora paradoxa and Basidioradulum molare from first incubation day of culture down to the levels of vehicle.

Comparison of Antioxidant Activities and Carotenoid Contents Between Marketable and Unmarketable Sweet Pepper Fruits (파프리카 상품과 및 비상품과의 항산화 활성 및 카로티노이드 함량 비교)

  • Yoon, Seungri;Kim, Jin Hyun;Shin, Minju;Jeong, Ho Jeong
    • Journal of Bio-Environment Control
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    • v.31 no.4
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    • pp.393-400
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    • 2022
  • Postharvest quality of sweet pepper fruits was mainly defined as external appearance, i.e., shape, fruit weight and surface color. These quality traits tend to involve esthetic appeal, it disregards gustatory properties and nutritional value. However, comparative studies according to the marketability of sweet pepper fruits are insufficient. This study was carried out to compare the physicochemical components, antioxidant activity and carotenoid contents of marketable and unmarketable sweet pepper fruits (Capsicum annuum L.). Physicochemical components (proximate composition, minerals and total phenolic contents) and antioxidant activities using various methods were investigated. The proximate composition values (%) of marketable and unmarketable fruits were: moisture (90.28 and 90.29), ash (0.74 and 0.26), crude protein (0.67 and 0.72), crude lipid (0.38 and 0.32). There were no significant differences in antioxidant activity, while total phenolic content was higher in marketable fruit. Carotenoids contents were 29.3 ± 2.6 and 31.9 ± 2.9 ㎍·g-1 in marketable and unmarketable fruits respectively, and identified β-carotene, violaxanthin, neoxanthin, and zeaxanthin. Lutein and capsaicin were not detected. In this study, potential value of unmarketable sweet pepper fruit could be identified to be applied as a food ingredient and functional food material.

Effect of Metalaxyl Seed-soaking Treatment on Root-mat Formation of Infant Rice Seedling in Machine Transplanting (벼 기계이앙 어린모 맷트형성 촉진을 위한 Metalaxyl 종자침종 효과)

  • Kim, Je-Kyu;Shin, Jin-Chul;Lee, Moon-Hee;Lim, Moo-Sang;Oh, Yun-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.4
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    • pp.287-293
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    • 1991
  • The root-mat formation of infant rice seedlings (8- to 10-day-old) is sometime not enough for machine transplanting because of the too-short nursery period. This experiment was conducted to elucidate the effect of metalaxyl seed-soaking treatment in the mixed solution of metalaxyl, seed disinfectant and insectcides on the root-mat formation of infant rice seedling in machine transplanting. The rice seeds of Odaebyeo were soaked in the mixed solution of metalaxyl, prochloraz and insectcides. with the recommended concentrations for 24 hours at room temperature. Seeding rate was 220g per seed tray (30 x 60 x 3cm). Metalaxyl (25% wettable powder), a fungicide. was used in 1. 000 times dilution as a promoting substance on the root-mat formation. Generally, the metalaxyl-treated seeds markedly increased the root number and length, and rooting activity of infant rice seedlings as compared with the control, thus the root-mat formation was excellent. When the rice seeds were sterilized by the mixed solution of prochloraz and insectcides, metalaxyl could be used together for increasing root-mat rormation of infant rice seedlIngs due to no interaction among agro-chemicals used. Seedling damping-off and physiological seedling rot were also controled in the seedlings of metalaxy-treated seeds. The root-mat of metalxyl-treated seeds had higher hardness and tension than control in terms of physical properties.

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Minimum Raising Duration of Infant Rice Seedling for Machine Transplanting (벼 기계이앙용 어린모 최소 육묘기간)

  • Kim, Je-Kyu;Lee, Moon-Hee;Oh, Yun-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.1
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    • pp.59-67
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    • 1992
  • The duration of raising seedling of infant rice seedling(IRS) in machine transplanting is 8 to 10 days. This experiment was conducted to investigate the minimum duration of IRS's raising seedling and its method by the combination of several treatments such as plant growth regulator, root-break-seat and temperature. The rice seeds of Hwaseongbyeo was soaked in the solution of metalaxyl for 24 hours at room temperature. Metalaxyl (25% wettable powder), a fungicide, was used in 1, 000 times dilution as a promting substance on the root-mat formation of IRS. The application of fungicide in the nursery soil for the controlling of damping-off and physiologyical seedling rot was omitted due to the metalaxyl seed-soaking treatment. Seeding rate was 220g per seed tray (30$\times$60$\times$3cm). To promote the root-mat formation of IRS, the sheets of polyethylene vinyl and absorptive paper were placed bottom the seed tray. The root-mat formation of IRS was promoted at higher temperature and longer duration of raising seedling. The metalaxyl-treated seeds markedly increased the root length and rooting activity of IRS as compared with the control, thus the root-mat formation was excellent. The absorptive paper with polyethylene vinyl as root-break-seat showed a better root-mat formation compared with control, polyethylene vinyl, alone. The minimum duration of raising seedling of IRS was 5 days after sowing based on the root-mat formation and seedling height under the condition of metalaxyl seed treatment, absorptive paper with polyethylene vinyl as a root-break-seat and the raising seedling temperature 30/2$0^{\circ}C$ (day /night).

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Effects of GA3 Dipping Treatment on the Spike Growth and Fruit Quality at Harvest of 'Campbell Early' Grapevine ('캠벨얼리' 포도의 화수(花穗) 생장과 과실품질에 미치는 GA3의 영향)

  • Lee, Y.C.;Moon, B.W.;Nam, K.W.;Moon, Y.J.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.15 no.1
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    • pp.85-93
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    • 2013
  • This study has been conducted to investigate the effect of GA3 solution dipping treatment after 1 days full bloom on spike growth and fruit quality at harvest of 'Campbell Early' grapevine. The fruit cluster growth(weight, length, width) and fruit stalk(diameter, length) at harvest were increased significantly by GA3 10 mg·L-1 solution dipping treatment compared to control and another concentration. The berry growth (berry No, weight, length, diameter) at harvest showed no difference by treatment GA3 concentration different. Spike weight was increased by GA3 5, 10, 20, mg·L-1 treatment compared to control, but no difference GA3 40 mg·L-1 treatment. The degree of compact berry, bloom, skin color and SSC at harvest by GA3 solution dipping treatment were not significantly different from those of control. but increased seedlessness induction by GA3 10 mg·L-1 treatment. The occurrence percent of berry cracking by GA3 5, 10, 20, 40 mg·L-1 treatment significantly decreased. The bitter rot of berry harvest showed no difference by treatment GA3 concentration different. Total spike length was increased by GA3 5, 10 mg·L-1 treatment compared to control and GA3 20, 40 mg·L-1 treatment. The spike length of 1st from 15th to position increased by GA3 10 mg·L-1 treatment.

Effects of tree-spray of organic calcium agent on the mineral nutrition concentration of petiole, fruit skin and fruit quality at harvest in 'Campbell Early' grapevine (포도 '캠벨얼리'에서 유기칼슘제 수관살포에 의한 엽병과 과피의 무기성분 농도 및 과실품질)

  • Moon, B.W.;Lee, Y.C.;Nam, K.W.;Koo, J.J.;Jung, H.W.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.13 no.1
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    • pp.35-46
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    • 2011
  • The effects of organic calcium agent(ECa) tree-spray on the mineral nutrition concentration of petiole and fruit skin and quality in grapevines were studied. The calcium concentration of petiole and fruit skin at harvest in 'Campbell Early' by tree-spray after flowering 14days of calcium agent were increased significantly in calcium chloride 0.4%, ECa 250X and MCa 500X compared to control. The calcium concentration of petiole and fruit skin at harvest by ECa tree-spray after 7 days and 14 days flowering were increased significantly compared to control and 21 days after flowering treatment. Also, The calcium concentration of petiole and fruit skin at harvest by tree-spray of ECa 250X and 125X after flowering were increased significantly compared to control and 500X treatment. Fruit quality, fruit cluster weight, cracking fruit and Bitter rot occurrence by ECa tree-spray of treatment time and concentration different showed no difference between control. Phytotoxicity by tree-spray of ECa concentration different not occur to leaf and fruit.

Evaluation of Nutrient Condition and Fruit Quality of 'Campbell Early' Grapevines in Hwaseong Area (화성지역 '캠벨얼리' 포도원의 수체영양과 과실품질의 평가)

  • Lee, Y.C.;Moon, B.W.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.5 no.1
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    • pp.28-35
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    • 2003
  • This study was conducted to investigate the nutrition status, growth and fruit quality of 'Campbell Early' grapes in Hwaseong area. Organic matter content of 3 vineyard soils was significantly lower than standard value. P2O5 and K contents of Y vineyard and P2O5 of C vineyard were very high. But there were no significant differences in contents of Ca, Mg and CEC. Compared to the standard value. T-N, Ca and Mg contents of petiole were adequate. P2O5 contents of Y and C vineyard and K contents of Y vineyard were significantly higher than standard value. Berry firmness, color and bloom occurrence on fruit skin showed no differences among 3 vineyard. Soluble solids content poorly, yield per tree and goods fruit of C vineyard decreased significantly compared to the fruit in Y and H vineyards. Russetted fruit of Y vineyard, colored fruit of H vineyard, and unfertilized fruit, cracked fruit, and bitter rot were observed frequently.

Changes in Physical Properties and Wood Chemical Components of sawdust medium during Oak Mushroom (Lentinula edodes) Cultivation (표고 재배 중 톱밥 배지의 물리적 성질과 목재 화학성분 변화)

  • Jong-Shin Lee;Seog-Goo Kang;Seung-Min Yang;Jin-Kyoung Kim
    • The Korean Journal of Mycology
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    • v.50 no.4
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    • pp.291-300
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    • 2022
  • In this study, the physical properties of the medium and changes in the wood chemical composition of the sawdust were investigated during the cultivation of oak mushroom sawdust bags, and the following results were obtained. After inoculation, the weight of the medium decreased during the incubation period. It is determined that this is not due to evaporation of moisture containing the medium or decomposition of sawdust, but to decomposition of rice bran, a low molecular substance added to the medium. It was confirmed that the moisture content of the medium was steadily increased during incubation, and it was estimated that the organic substrates such as rice brane in the medium was decomposed by mycelium, and water, one of the decomposition products of organic substrates, caused an increase in the moisture content of the medium. Along with the increase in the harvest of oak mushrooms, the proportion of organic substances such as holocellulose and lignin, the main components of the wood cell wall of sawdust, steadily decreased. In particular, the degradation characteristics of the wood cell wall component of shiitake, which is a white rot fungi, were confirmed by higher lignin reduction rate than that of holocellulose. On the other hand, ash, which is an inorganic material, increased with an increase in the number of mushroom harvests. The increase in the amount of ash in the medium may have been due to the decrease in the organic matter content such as holocellulose and lignin.

Optimization of In vitro Cultures for Production of Seedling and Rootstock of Rehmannia glutinosa(Gaertn.) DC. (지황 배양묘 및 종근 생산을 위한 기원검증 및 최적기내배양조건 확립)

  • Kang, Young Min;Lee, Ka Youn;Kim, Mi Sun;Choi, Ji Eun;Moon, Byeong Cheol
    • Journal of agriculture & life science
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    • v.50 no.5
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    • pp.81-93
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    • 2016
  • Rehmannia glutinosa(Gaertn.) DC. is a herbaceous perennial plant and belonging to the Scrophulariaceae and used as roots for medicinal part and purpose. R. glutinosa is and usually used for fresh rehmannia root or prepared rehmannia root. However, it is very difficult to propagate using the seeds because of lack germination so it is propagated using the vegetative method as the rootstock. Currently, propagation and harvesting using the rootstock of R. glutinosa has difficulties about production of the high quality and quantity in R. glutinosa because of root rot disease. To optimize in vitro cultures and to improve the rootstock and seedling of R. glutinosa after morphological and genetical determination, 5 plant culture media (MS, DJ, LS, QL, and WPM) were used in this study then WPM was selected for better growth, for multiplication condition(WPM + IAA 1.0 mg/L + IBA 0.5 mg/L), and for root enlargement condition(WPM + NAA 0.1 mg/L) of R. glutinosa. Based on these results, in vitro seedlings of R. glutinosa were transferred to soil for acclimation with environment adaptation and shown the positive effects about root enlargement and root formation. Therefore, it can be used for high quality of R. glutinosa production and production of the rootstock based on propagation using in vitro seedlings of R. glutinosa.

Genome Wide Association Study for Phytophthora sojae Resistance with the Two Races Collected from Main Soybean Production Area in Korea with 210 Soybean Natural Population

  • Beom-Kyu Kang;Su-Vin Heo;Ji-Hee Park;Jeong-Hyun Seo;Man-Soo Choi;Jun-Hoi Kim;Jae-Bok Hwang;Ji-Yeon Ko;Yun-Woo Jang;Young-Nam Yun;Choon-Song Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.202-202
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    • 2022
  • Recently days, soybean production in paddy field is increasing, from 4,422 ha in 2016 to 10,658 ha in 2021 in Korea. It is easy for Phytophthora stem and root rot (PSR) occurring in paddy field condition, when it is poorly drained soils with a high clay content, and temporary flooding and ponding. Therefore PSR resistant soybean cultivar is required. The objective of this study is to identify QTL region and candidate genes relating to PSR resistance of the race in main soybean cultivation area in Korea. 210 soybean materials including cultivars and germplasm were used for inoculation and genome-wide association study (GWAS). Inoculation was conducted using stem-scar method with 2 replications in 2-year for the race 3053 from Kimje and 3617 from Andong. 210 materials were genotyped with Soya SNP 180K chip, and structure analysis and association mapping were conducted with QTLMAX V2. The results of inoculation showed that survival ratio ranged from 0% to 96.7% and mean 9.7% for 3053 and ranged from 0% to 100% and mean 7.6% for 3617. Structure analysis showed linkage disequillibrium (LD) was decayed below r2=0.5 at 335kb of SNP distance. Significant SNPs (LOD>7.0) were identified in Chr 1, 2, 3, 4, 5, 11, 14, 15 for 3053 and Chr 1, 2, 3, 7, 10, 14 for 3617. Especially, LD blocks (AX-90455181;15,056,628bp~AX-90475572;15,298,872bp) in Chr 2 for 3053 and 3067 were duplicated. 29 genes were identified on these genetic regions including Glyma.02gl47000 relating to ribosome recycling factor and defense response to fungus in Soybase.

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