• 한국지능정보시스템학회:학술대회논문집
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• 한국지능정보시스템학회 2002년도 추계정기학술대회
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• pp.423-431
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• 2002

본 연구에서는 웹 서비스 기술을 이용하여 B2B Exchange 구현을 위한 프레임웍을 설계하고 XML과 SOAP기술을 이용하여 이를 구현하였다. B2B e-Commerce가 주도적인 회사 중심의 B2B인데 반해서 B2H Exchange는 다수의 구매자와 판매자가 하나의 가상 시장에서 역동적으로 거래가 행해지는 상태를 나타내므로 참가자간에 네트웍 효과가 발생한다. 이를 구현하기 위해서는 어느 하나의 프로토콜로서는 용이하지 않으며 현재까지 ebXML, BizTalk, SOAP, RosettaNet, Eco Framewok, OPT 등의 프로토콜이 제시되어 표준화를 위한 노력을 해 오고 있다. 본 연구는 B2B Exchange를 구현하기 위해서 UDDI, SOAP, WSDL로 구성된 웹 서비스를 이용한 프레임을 설계하고 Apache 서버, SOAP 및 Xerces 환경에서 프로토타입을 구현하였다.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2001년도 춘계학술발표논문집 (하)
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• pp.793-796
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• 2001

XML은 강력한 데이터 표현능력을 인정받아 전자상거래와 같은 데이터 처리 분야에 적극적으로 도입되고 있다. 하지만 XML을 이용하여 다른 조직이나 사용자간에 원활한 데이터 교환과 사용을 위해서는 공통적으로 사용할 수 있는 태그나 용어가 표준화되어야 한다. 현재 이와 같은 표준화 작업은 CommerceNet에서 주관하는 xCbl이 주를 이루고 있으며, RosettaNet과 BizTalk에서도 자신의 고유한 문서 표준을 채택하고 있다. 하지만 위의 표준은 B2B간의 상품이나 서비스에 대한 포괄적인 관점으로 기술되었기 때문에 상품 자체의 세부적인 정보 표현에는 부족한 점이 있다. 또한 현재 국내에서 이와 같은 표준에 대한 구축이 초기 단계에 있기 때문에 산업별, 산업간 정보 연계를 위한 표준화가 시급하다. 본 논문에서는 이와 같은 표준화 작업의 일환으로 XML에 기반 하여 가능한 모든 상품을 기술할 수 있는 표준 모델을 제시하고 이를 XML의 DTD로 구현하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2002년도 추계학술대회 논문집
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• pp.93-96
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• 2002

The manufacturing paradigm has shifted dramatically over the past decade from “push” or mass production mode to “pull” or customer-driven, order-based manufacturing mode, as multitudes of customers now demand mass customization of configurable products. As a means to achieve such rapidly responsive manufacturing system, Advanced Planning System (APS) has become an essential software tool for achieving modern “build-to-order” and “configure-to-order” manufacturing environment. APS enables manufacturers to respond to variety of customer demands In real time by instantly configuring manufacturing processes based on specifications described in each purchase orders and providing capable-to-promise information directly to customer by performing rapid “what-if” manufacturing simulated scenarios. This paper discusses the working of such system as well as the business processes that incorporate such systems to enable efficient “build-to-order” environment.

• Genomics & Informatics
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• 제6권4호
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• pp.202-209
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• 2008

Biological pathways are known as collections of knowledge of certain biological processes. Although knowledge about a pathway is quite significant to further analysis, it covers only tiny portion of genes that exists. In this paper, we suggest a model to extend each individual pathway using a microarray expression data based on the known knowledge about the pathway. We take the Rosetta compendium dataset to extend pathways of Saccharomyces cerevisiae obtained from KEGG (Kyoto Encyclopedia of genes and genomes) database. Before applying our model, we verify the underlying assumption that microarray data reflect the interactive knowledge from pathway, and we evaluate our scoring system by introducing performance function. In the last step, we validate proposed candidates with the help of another type of biological information. We introduced a pathway extending model using its intrinsic structure and microarray expression data. The model provides the suitable candidate genes for each single biological pathway to extend it.

• 천문학회지
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• 제38권2호
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• pp.253-256
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• 2005

We have constructed the Mount Fuji submillimeter-wave telescope at Nishiyasugawara (alt. 3725 m) near the summit of Mt. Fuji (alt. 3774 m). Thanks to the excellent condition of Mt. Fuji, we have successfully carried out the [CI] survey toward more than 40 square degrees of sky, including qrion MC, Taurus MC, Rosetta MC, DR 15, DR 21, NGC 1333, NGC 2264, W 3, W 44, W 51, L 134, p-Oph. Our [CI] survey have revealed that the [CI] 492 GHz emission widely extends to the molecular clouds. The spatial and velocity structures of the [CI] 492 GHz emission resemble those of 13CO J=l-0 in many molecular clouds, implying that [CI] 492 GHz and $^{13}CO$ J=1-0 are emitted from the same gas. The column density of $C^o$ linearly correlates with that of CO up to high Av, suggesting that $C^o$ exist in the deep interior of molecular clouds. In several regions, we have found that the distributions of $C^o$ and CO are different from each other. The $C^o$-rich area is found in the Hieles' cloud 2. The C+/CO/$C^o$ configuration is found in DR 15, p-Oph, M 17, Orion KL, and NGC 1333. These results indicate that an origin of $C^o$ is unrelated with the photodissociation process. We discuss the observed $C^o$ distributions in relation to the non-equilibrium chemistry.

• Animal cells and systems
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• 제16권6호
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• pp.455-461
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• 2012

Amongst the various antimicrobial peptides, lysozyme plays a central role in initiating and maintaining the antibacterial defense response of insect. Here we propose the biosynthesis and refolding of recombinant lysozyme in Escherichia coli expressed in inclusion body form. The Agrius lysozyme gene was amplified using gene specific primers and then ligated into the pGEX-4T-1 vector, which contained the glutathione S-transferase (GST) gene as a fusion partner. A recombinant lysozyme was expressed in E. coli Rosetta cells using a pGEX-4T-1 expression vector, and the fusion protein was induced by ioporpyl-${\beta}$-D-thiogalactopyranoside (IPTG). The recombinant protein produced as an inclusion body was resolubilized in solubilization buffer, and the resultant solution was dialyzed in refolding buffer. After thrombin cleavage, the recombinant lysozyme was purified by ion exchange chromatography and reverse phase chromatography. The recombinant lysozyme was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis and immunoreactivity against the anti-Agrius lysozyme was observed by western blot analysis of this protein. The recombinant lysozyme displayed antibacterial activity against Bacillus megaterium and Micrococcus luteus, which was confirmed by the inhibition zone assay.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2001년도 춘계학술대회 발표논문집
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• pp.370-373
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• 2001

전자상거래는 사람들에게 인터넷을 통하여 상품과 서비스를 구매할 수 있게 하였다. 초기의 전자상거래는 일반 소비자를 주요 판매 대상으로 하였다 그러나, 각각의 기업에서 인터넷이 모든 종류의 비즈니스 창구가 될 수 있음 인식하고 관심을 보이기 시작했다. 기업을 대상으로 하는 전자상거래를 B2B(Business to Business)라 한다. 그러나, B2B 분야가 아무리 더 큰 이익을 창출할 수 있다고 해도, 이를 실현하는데는 많은 문제점이 존재하고 있다. 현재 B2B 분야에서는 포괄적이면서도 단일화된 거래환경의 지원이 무엇보다 시급한 문제로 떠오르고 있다. 원활한 B2B 전자상거래 이루어지기 위해서는 세계적으로 인정되는 표준을 바탕으로 완벽한 상호운용성이 보장되며, 기업 내부의 환경에 효과적으로 대응할 수 있는 프레임워크 기술이 절실히 요구된다. 이러한 기업간 전자상거래의 문제를 해결하고자 하는 목적으로 많은 기업과 단체에서는 각자의 표준을 정립하고, 이를 지원하기 위한 프레임워크를 진행하고 있다. 현재까지 발표된 대표적인 프레임워크로는 CommerceNet의 eCo 프레임워크, RosettaNet, Microsoft의 BizTalk 프레임워크, UN/CEFACT와 OASIS의 듀 XML이 있다. 이들 대부분의 프레임워크들은 XML과 인터넷을 기반으로 하는 것이 특징이다. 본 고에서는 기업간 전자상거래에 관심을 가지고 있는 기업들에게 현재의 기술동향과 향후 방향 설정에 있어서 도움이 될 수 있기 위한 목적으로 현재 발표된 XML 기반 전자상거래 프레임 워크 기술과 향후전망에 대하여 기술한다.

• 천문학회보
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• 제44권2호
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• pp.45.1-45.1
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• 2019

Comet 46P/Wirtanen is a Jupiter-family comet whose orbital period is of approximately 5.44 years and perihelion lying at about 1.06 au. The comet is known for being a primary target of the Rosetta space mission prior to it being rescheduled to 67P/Churyumov-Gerasimenko. In its 2018 apparition, comet 46P approached Earth within ~0.08 au, which made possible its study with relatively small telescopes. We used this rare opportunity to conduct a comprehensive study of the 46P polarization from November 16, 2018, about a month prior to its perihelion passage December 12, until January 17, 2019. Over this two-month time period, weather conditions were favorable on 13 nights. Observations were made with the 22-cm telescope located at the Ussuriysk Astrophysical Observatory (code C15), which operates within the International Scientific Optical Network (ISON). We will report our findings at the conference.

• KSBB Journal
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• 제27권4호
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• pp.257-262
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• 2012

The gene encoding Thermus thermophilus HJ6 laccase (Tt-laccase) was cloned, sequenced, and comprised of 1,389 nucleotides encoding a protein (462 amino acids) with a predicted molecular mass of 51,049 Da. The deduced amino acid sequence of Tt-laccase showed 99.7% and 44.3% identities to the Thermus thermophilus HB27 laccase and Synechococcus sp. RS9917 laccase, respectively. Tt-laccase gene was expressed as a fusion protein with six histidine residues in E. coli Rosetta-gami (DE3) cells, and the recombinant protein was purified to homogeneity. UV-Vis spectrum analysis revealed that the enzyme has copper atoms, a type I Cu(II) and a type III binuclear Cu(II). The optimum pH for the oxidation of guaiacol was 5.0 and the optimum temperature was $90^{\circ}C$ The half-life of heat inactivation was about 120 min at $90^{\circ}C$ The enzyme reaction was inhibited by sodium azide, L-cystein, EDTA, dithiothreitol, tropolone, and kojic acid. The enzyme oxidized various known laccase substrates, its lowest $K_m$ value being for 4-hydroxyindole, highest $k_{cat}$ value for syringaldazine, and highest $k_{cat}/K_m$ for guaiacol.

• BMB Reports
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• 제53권2호
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• pp.118-123
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• 2020

Cardiac regeneration with adult stem-cell (ASC) therapy is a promising field to address advanced cardiovascular diseases. In addition, extracellular vesicles (EVs) from ASCs have been implicated in acting as paracrine factors to improve cardiac functions in ASC therapy. In our work, we isolated human cardiac mesenchymal stromal cells (h-CMSCs) by means of three-dimensional organ culture (3D culture) during ex vivo expansion of cardiac tissue, to compare the functional efficacy with human bone-marrow derived mesenchymal stem cells (h-BM-MSCs), one of the actively studied ASCs. We characterized the h-CMSCs as CD90^low, c-kit^negative, CD105^positive phenotype and these cells express NANOG, SOX2, and GATA4. To identify the more effective type of EVs for angiogenesis among the different sources of ASCs, we isolated EVs which were derived from CMSCs with either normoxic or hypoxic condition and BM-MSCs. Our in vitro tube-formation results demonstrated that the angiogenic effects of EVs from hypoxia-treated CMSCs (CMSC-Hpx EVs) were greater than the well-known effects of EVs from BM-MSCs (BM-MSC EVs), and these were even comparable to human vascular endothelial growth factor (hVEGF), a potent angiogenic factor. Therefore, we present here that CD90^lowc-kit^negativeCD105^positive CMSCs under hypoxic conditions secrete functionally superior EVs for in vitro angiogenesis. Our findings will allow more insights on understanding myocardial repair.