• Korean Journal of Veterinary Service
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• v.22 no.2
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• pp.159-167
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• 1999

This survey was conducted for the serological confirmation on large outbreak of bovine brucellosis in two dairy farms. Serological tests were performed by the plate agglutination test, tube agglutination test, enzyme linked immunosorbent assay(ELISA), complement fixation, ,test(CFT) and rose bengal plate test(RBPT). Total 200 heads(134 heads in farm A and 66 heads in farm B) were tested. The primigravida and positive group have been raised separately in the farm A and both group have been raised together in the farm B.. The result were summarized as follows ; 1. Positive ratios in positive herds of farms by the tube agglutination test were 68.3% in farm A and 53.2% in farm B. 2. Seroconversion to brucella was observed in the primigravidas group in farm B, but was not observed in the primigravidas group in farm A. 3. All calves born in positive herd were serologically negative at time of test. 4. Positive ratio of ELISA in farm A was higher than that of tube agglutination test. 5. Number of positive reactors by the CFT, RBPT in farm A were equal to those of tube agglutination test.

• Korean Journal of Veterinary Research
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• v.54 no.4
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• pp.239-243
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• 2014

This study was conducted to investigate the status of brucellosis in dairy cattle from five selected dairy farms in the Mohammadpur Beribadh area of Bangladesh. A cross-sectional study was carried out from October 2010 to March 2011 in which a total of 334 serum samples from cattle in five herds were screened by the Rose-Bengal plate-agglutination test (RBPT) and the positives were confirmed using an indirect enzyme-linked immunosorbent assay (I-ELISA). A structured questionnaire was used to collect epidemiological information describing the animals. Overall, 4.20% of the animals were RBPT positive, while subsequent confirmatory tests with I-ELISA revealed that the overall animal-level prevalence derived from the samples was 1.20%. Additionally, the prevalence was relatively higher in females than in males. A significant association was found between abortion, age of the animals, and the occurrence of brucellosis (p < 0.05). Considering the overall low prevalence of brucellosis in the selected farms in the present study, a brucellosis eradication program for dairy farms using a test-and-slaughter policy would be possible.

• Korean Journal of Veterinary Service
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• v.31 no.4
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• pp.493-496
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• 2008

Brucella spp. are small, non-motile Gram-negative coccobacilli known to cause disease in a number of vertebrate species including humans and brucellosis is one of the world's major zoonoses, alongside bovine tuberculosis and rabies. There are about 33.55 million goats and 1.16 million sheep in Bangladesh. The sheep and goats can significantly play an important role in the economic well being of the resource-poor farmer in Bangladesh. Sexually matured 362 female small ruminants(300 goats and 62 sheep) were examined. Approximately 3-5 ml of blood was collected from the jugular vein of each animal and sera samples were prepared. Samples were then tested for brucellosis by using Rose Bengal test(RBT), plate agglutination test(PAT) and tube agglutination test(TAT). Among 362 small ruminants, irrespective of species(sheep or goat), diagnosed highest in TAT, 2.21%(n=8) and lowest both by RBT & PAT, 1.93%(n=7) and it is concluded that TAT is superior than RBT and PAT.

• Korean Journal of Veterinary Service
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• v.33 no.1
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• pp.29-35
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• 2010

This study was performed to test the hypothesis that Brucella abortus strain RB51 (SRB51) might protect Korean indigenous mongrel dog against challenge with either virulent B. abortus biotype 1 or B. canis. A total of 12 Korean mongrel dogs were divided into four groups (Group A, B, C and D). Dogs belonging to Group A and C were inoculated subcutaneously with $1{\times}10^9$ CFU of SRB51 in 1ml of sterile phosphate buffered saline (PBS). Dogs of Group B and D were inoculated subcutaneously with 1ml of sterile PBS as control. At 12 weeks post vaccination, dogs of Group A and B were challenged by oral inoculation of virulent strain of B. canis ($5.0{\times}10^9$ CFU) and dogs of Group C and D were challenged by oral inoculation of virulent strain of B. abortus biotype 1 ($4.4{\times}10^{10}$ CFU). The serum antibodies titers in all dogs were monitored at regular interval for eight weeks after challenge (AC) by standard tube agglutination test, plate agglutination test, rose bengal test, 2-mercaptoethanol rapid slide agglutination test and 2-mercaptoethanol tube agglutination test. No antibody titers in Group A and C was detected. Also, the challenge strains were not found from blood of all dogs of Group A and C from 1 week AC till the end of the experiment by culture and modified AMOS-PCR, whereas B. canis and B. abortus challenge strains were detected from blood of Group B and D, respectively. In addition, neither of two challenge bacteria was recovered from liver, spleen, kidneys, lymph nodes and reproductive tracts of Group A and C dogs after postmortem. However, B. canis and B. abortus challenge strains were isolated from these tissues of Group B and D, respectively. These data suggest that SRB51 could be a promising vaccine candidate for immunizing dogs to control canine brucellosis caused by B. canis or B. abortus.

• Korean Journal of Veterinary Service
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• v.34 no.2
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• pp.159-166
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• 2011

This study was carried out to develope enzyme-linked immunosorbent assay (ELISA) for detection of canine brucellosis in dogs experimentally inoculated with Brucella abortus 1119-3 and B. canis RM666. Groups A, B and C of dogs (each group consisting of three dogs) were orally inoculated with approximately $5{\times}10^9$ colony-forming units of B. abortus and B. canis, and with sterile pyrogen-free PBS, respectively. The animals were monitored at regular intervals upto the 12th week post inoculation (PI) by standard tube agglutination test (STAT), plate agglutination test (PAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and ELISA. The induced antibody titers in group A dogs were detected from the first week PI to the eighth week PI in STAT, PAT and RBT using the inactivated whole cells of B. abortus 1119-3 as antigens, while no sera in groups B and C dogs reacted with the antigens. In 2ME-RSAT using whole cells of B. canis M-strain as antigens, the induced antibody titers in group B dogs were observed at the second week PI and persisted for the 12th week PI, while sera of groups A and C dogs did not react with the whole cells. In ELISA using cytoplasmic fractions antigen of B. abortus 1119-3, the mean optical density of antibodies in groups A and B was detected from the first and second weeks PI, respectively, and persisted for 12th week PI, while sera of group C did not cross-react with the fractions antigen. However, in ELISA using the hot saline extracts of B. canis M- as an antigen, the induced antibody titers in only group B dogs were detected from second week PI and persisted for until the end of this study. These results indicate that the ELISA using B. abortus 1119-3 cytoplasmic fractions as antigens can be a good candidate for detection of brucellosis by B. abortus as well as B. canis in dogs.

• Korean Journal of Veterinary Service
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• v.30 no.4
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• pp.511-525
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• 2007

A seroprevalence study of small ruminant brucellosis was conducted in sheep and goat rearing selected areas of Mymensingh district and Dhaka district, Bangladesh, from March, 2005 to May, 2006. Sera from 62 sheep and 300 goats were tested by rose bengal plate test (RBPT), plate agglutination test (PAT), tube agglutination test (TAT) and mercaptoethanol test (MET). Out of the 62 sera tested 3.25% (n = 2) were positive to RBT, PAT and TAT and 4.84% (n = 3) were positive MET. In case of 300 goats, 1.67% (n = 5) were positive to RBT and PAT, 2% (n = 6) were positive to TAT and 2.33% (n = 7) were positive to MET. This investigation is the first of its type to be performed in small ruminants in Bangladesh. Higher prevalence rate (8.0 %) was found in BAU nutrition farm in case of sheep and 10 % in Bangladesh Agricultural University (BAU) Veterinary Clinic in case of goat while lower prevalence (0.0 %) was recorded in Pharmacology project and BAU adjacent villages in case of sheep and (0.0 %) in Dhamrai upazila in case of goats respectively. Brucella antibodies were more prevalent in sheep (8.84 %) than in goat (2.33 %).

• Korean Journal of Veterinary Service
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• v.33 no.2
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• pp.121-128
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• 2010

Brucellosis is a serious zoonosis, recognized worldwide. It primarily affects animals, which act as reservoirs for human infection as well as being of economic significance to the agri-food industry. Bangladesh has been reported as an endemic area for brucellosis. So a cross sectional study was conducted to determine the seroprevalence and potential risk factors of brucellosis in cattle in Dinajpur and Mymensingh districts of Bangladesh. A total of 182 cattle were examined by Rose Bengal Plate Test (RBPT) between September 2008 and October 2009. Then Positive, doubtful, and negative samples were further confirmed with slow agglutination test (SAT) and both indirect and competitive enzyme linked immunosorbent assay (iELISA and cELISA). A questionnaire was used to collect epidemiological information of the animals. The overall animal-level prevalence was 3.30%. Brucellosis seroprevalence was higher (4.76% by cELISA) in cattle above 48 months than those under 48 months. Female showed higher seroprevalence (10.67%) than male (6.25%). Higher seroprevalence was also found in cattle bred naturally (20.0%) than artificially (8.77%) and cattle that aborted or with previous abortion record (22.22%) showed higher seroprevalence than non-aborted (7.69%). The sensitivity of RBT and SAT was found 100% as compared to cELISA standard test, whereas specificity of RBT (95.35%) was higher than that of SAT (94.32%).

• Korean Journal of Veterinary Service
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• v.30 no.2
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• pp.259-267
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• 2007

A study on the relation between some husbandry practices and brucellosis in goats in Bangladesh was conducted at selected areas of Mymensingh and Dhaka district, Bangladesh, from March 2005 to May 2006. Sera from 300 goats were tested by Rose bengal test (RBT), plate agglutination test (PAT), tube agglutination test (TAT) and mercaptoethanol test (MET). Out of the 300 goats, 1.670% (n=5) were positive to RBT and PAT respectively, and 2.0% (n=6) were positive to TAT and 2.33% (n=7) were positive to MET. The prevalence of brucellosis was bigger in goats reared collectively (n=2, 4%) than reared individually (n=5, 2%), and bigger in goats housed with concrete floor (n=2, 4%) than that of bare floor (n=5, 2%). The rate of brucellosis was higher in goats keep separately (n=6, 2.61%) than that of kept with other animals (n=1, 1.43%) especially with cattle. Out of 290 goats from free grazing, 7 were positive but no positive reactor(n=10) was found in non grazing goats. In conclusion, however, seroprevalence of brucellosis had no statistically significant association with rearing type, housing type and grazing or not.