• 한국환경농학회지
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• 제35권2호
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• pp.137-142
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• 2016

BACKGROUND: Identification of endophytic yeasts inhabiting the internal roots of the Mankyua chejuense tree requires techniques involving biotechnology. There is a need for a culture-based method to isolate and identify yeast strains associated with M. chejuense.METHODS AND RESULTS: We spread homogenized M. chejuense root samples onto glucose-peptone- yeast agar containing antibiotics, Triton X-100, and L-sorbose. A total of 152 yeast isolates were obtained and identified via phylogenetic analysis based on ITS gene sequencing. The results revealed that the root-associated yeast species included the genera Cyberlindnera (140 isolates), Candida (11 isolates), and Kluyveromyces (one isolate). Additionally, three yeast isolates showed high bioethanol production.CONCLUSION: We identified the specific yeast community associated with M. chejuense roots. These yeast isolates may have industrial applications as bioethanol producers. Our findings revealed that Cyberlindnera isolates included C. suaverolens and C. satumus, while Kluyveromyces isolates showed high bioethanol production.

• Current Research on Agriculture and Life Sciences
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• 제32권2호
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• pp.75-78
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• 2014

Burdock (Arctium sp.) is known as a nutraceutical vegetable, especially in Japanese and Korean cuisine. While burdock plants are generally harvested for their tap roots, different parts of the plant are consumed as food or used as traditional medicines. This study investigated the physicochemical properties of the leaves, stems, roots, and peeled roots of the burdock plant based on their pH, soluble solid content, titratable acidity, color values, and mineral content. The pH differed significantly among the different plant parts, with the highest value in the leaves and the lowest in the stems. However, for the soluble solid content, the leaves had the lowest, while the peeled roots had the highest. The titratable acidity of the stems was significantly lower than that of the leaves, roots, and peeled roots. As regards the color values, the lightness value was highest for the stems, while the roots showed the highest redness value, followed by the peeled roots, and the leaves had the highest yellowness value. The leaves and stems contained almost three times more potassium than the roots and peeled roots. Thus, the higher content of different minerals in the leaves and stems of the burdock plant shows that these plant parts could be used as potential sources of dietary minerals.

• 진공이야기
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• 제2권4호
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• pp.39-46
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• 2015

After stepping into a new field of vacuum 30 years ago, our company has grown up steadily as a specialized vacuum industry, and now we can provide vacuum devices covering most of the pressure range. We are planning to put out high level dry pump like a multistage Roots pump on the market in the near future. Procedures of technology development for designing, fabricating, and testing the multistage Roots pump of 600 L/min class will be briefly reported. Core items of the technical development on the multistage Roots pump are as follows; elaborated profile design of 3-lobe rotors using an involute curve, optimization of rotor dimensions, especially for clearances and rotor width, considering the pumping speed, compression ratio and heat load, and establishment of a standardized test system. At present, the multistage Roots pump is about to come into commercialization.

• 한국환경농학회지
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• 제36권2호
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• pp.135-139
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• 2017

BACKGROUND: Identification and characterization of endophytic yeasts inhabiting the roots of Ulmus parvifolia Jacq. and Quercus salicina Blume require biotechnological and culture-based techniques. METHODS AND RESULTS: Homogenized U. parvifolia and Q. salicina root samples were spread onto four types of agar medium containing ancgtibiotics, L-sorbose, and Triton X-100. In total, 25 yeast strains were isolated and subjected to phylogenetic analysis based on their internal transcribed spacer region sequences. The results revealed that the yeast genera Cyberlindnera (12 isolates) and Cryptococcus (1 isolate) were associated with roots of U. parvifolia; and the genera Rhodotorula (8 isolates), Trichosporon (3 isolates), and Kluyveromyces (1 isolate) were associated with roots of Q. salicina. Additionally, a Kluyveromyces isolate produced a detectable level of bioethanol. The yeast strains reported herein may be used in industrial production of biosurfactants and bioethanol. CONCLUSION: Our findings revealed that the endophytic yeast genera Cyberlindnera and Cryptococcus predominated in roots of U. parvifolia; and the genera Rhodotorula (8 isolates), Trichosporon (3 isolates), and Kluyveromyces (1 isolate) predominated in roots of Q. salicina. Additionally, Kluyveromyces isolates produced a detectable level of bioethanol.

• Applied Biological Chemistry
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• 제38권1호
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• pp.72-77
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• 1995

품질이 좋은 인삼의 생산을 위한 재배지포장의 토양 및 영양관리를 위한 기초자료를 얻기 위하여, 인삼재배지토양의 화학성과 인삼근 중의 전당, 환원당, 유리당류, ginsenoside 및 saponin들의 상관관계를 조사하여, 인삼근의 품질에 미치는 영향을 조사하였다. 토양 중 $NO_3-N$함량은 인삼근 중의 전당 및 환원당의 함량과 고도의 부의 상관이 인정되었다. 토양 중 유기물함량은 인삼근 중의 당류와 ginsenoside계 성분들의 함량과 정의 상관이 인정되었으나, 토양 중 유효인산 및 치환성 양이온들의 함량은 부의 상관이 인정되었다.

• 펄프종이기술
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• 제42권2호
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• pp.53-60
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• 2010

Kudzu vine (Pueraria lobata) is a perennial plant and spreads all around in South Korea. Recently the use of leaves, stems and roots of kudzu vine has been investigated in many fields. However, the research on kudzu fibers has not been performed in korean pulp and paper industry. As the stems and roots of kudzu were estimated to contain cellulosic fibers, the possibility of producing a raw material from kudzu fibers in pulp and paper mills was investigated in this study. The stems and roots of kudzu were collected in woods, and then the chemical composition and ash contents was measured. To acquire kudzu pulp, kraft pulping and bleaching of kudzu roots were carried out sequently. After making kudzu pulp, freeness and fiber length were measured, and handsheets was also prepared with kudzu pulp and the properties of handsheets were determined. Consequently, kudzu fibers have lower holocellulose contents than wood fibers and other non-wood fibers. Average fiber length is shorter than that of Sw-BKP, but shows the same level as that of Hw-BKP. The handsheet made from kudzu pulp shows the bulkier structure than those made from Hw-BKP and Sw-BKP.

• 한국식품저장유통학회지
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• 제19권1호
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• pp.31-36
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• 2012

신선절단 우엉의 제조에 있어 가공 온도와 갈변 억제제의 영향을 구명하기 위하여, $4^{\circ}C$와 $25^{\circ}C$에서 제조하거나, 각종 갈변 억제제(3% citric acid, 3% sodium chloride, 0.3% cystein, 3% sodium acetate 용액)에 침지 처리한 신선절단 제품의 품질 및 갈변 특성을 조사하였다. 가공온도 $4^{\circ}C$제품이 $25^{\circ}C$ 제품보다 호흡량과 갈변도는 낮았으며 가용성 고형분 함량은 높았다. 반면에 제품의 중량 감소율과 과육경도는 가공온도의 영향을 거의 보이지 않았다. 갈변 억제제의 종류에 따라 제품의 갈변도는 다르게 나타났으며 그 중 0.3% cystein 처리가 갈변을 가장 지연시키는 효과를 보였다. 제품의 페놀성 물질 함량은 갈변 억제제 처리 유무에 따른 큰 차이를 보이지 않았고, PPO 활성은 갈변 억제제 처리구보다 무처리구에서 비교적 높은 수준을 보였다. 이로써 가공 온도가 신선절단 우엉 제품의 갈변을 포함한 품질 특성에 영향을 미치는 것이 확인되었고, 고품질 제품을 위해서는 저온 가공에 적절한 갈변 억제제 처리가 병행되어야 할 것으로 여겨진다.

• 한국응용과학기술학회지
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• 제34권1호
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• pp.33-40
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• 2017

To isolate and identify the yeast strains associated with D. morbifera, homogenized D. morbifera root samples were spread onto GPY, DG18, SCG and DOB agar media containing antibiotics, Triton X-100, and l-sorbose. Total 81 yeast isolates were analyzed by sequencing of internal transcribed spacer (ITS) region of the ribosomal DNA. The results showed that the root-associated yeast species were composed of the genera Vanderwaltozyma (40 isolates), Cryptococcus (40 isolates), and Kluyveromyces (one isolate). Moreover, the Kluyveromyces isolate exhibited high bioethanol productivity. In addition, the Vanderwaltozyma and Cryptococcus were dominant in D. morbifera roots. The specific yeast community associated with D. morbifera roots was identified by phylogenetic sequence analyses. These yeast isolates may have industrial applications as biosurfactant and bioethanol.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.617-621
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• 2002

In order to optimize the carrot hairy root culture for SOD production, a fed-batch culture of hairy roots was performed in a bioreactor. Maximum SOD activity was obtained when the hairy roots were transferred to the MS medium containing 110 g/1 concentration of sucrose. By controlling the sucrose concentration (70 g/1 sucrose for growth and 110 g/1 sucrose far production, respectively) In a two-stage fed-batch culture, 29 g/1 of the hairy roots was obtained based on the final dry mass. The volumetrically determined SOD activity and productivity in the fed-batch culture were about 6 times higher than those from the flask culture containing sucrose at 30 g/1 concentration.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2007년도 춘계학술발표회
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• pp.209-210
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• 2007