• Journal of Nutrition and Health
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• v.49 no.1
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• pp.18-27
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• 2016

Purpose: Our previous study demonstrated the hypoglycemic effects of mulberry (Morus alba L.) leaf and the underlying mechanisms. Here we explored the potency of mulberry twigs (TW) and root barks (RB) in postprandial hypoglycemic effects in vitro and in vivo. Methods: The major components of TW and RB were determined by high performance liquid chromatography (HPLC). Alpha-glucosidase inhibition and glucose/fructose uptake inhibition in Caco-2 cells were determined for TW, RB, and their major components, followed by an oral sugar tolerance test (OSTT) in streptozotocin-induced diabetic rats. Male Wistar rats were fed a high-fat diet for 2 weeks and then a single dose of streptozotocin (35 mg/kg B.W) was administered by intraperitoneal injection. Rats with fasting blood glucose levels above 126 mg/dL were randomly divided into 5 groups (n = 8/group) for the following treatments by gavage for 4 weeks: vehicle (normal control and diabetic control), 200 mg/kg B.W of TW or RB or 100 mg/kg B.W of oxyresveratrol (OXY). Results: OXY and mulberroside A were identified as the major components of TW and OXY, mongolicin, and kuwanon H for RB. A significant inhibitory activity on ${\alpha}-glucosidase$ was found for TW, RB, and OXY (p = 0.0099). There was a dose-dependent inhibition of TW and RB on the intestinal sugar uptakes in Caco-2 cells, showing a greater impact on fructose compared to glucose. The OSTT showed that TW and RB significantly delayed time to maximal concentration (p = 0.0088) and decreased maximal concentration (p = 0.0043) compared to the control group. Conclusion: These results suggest that TW and RB may have a postprandial hypoglycemic effect, particularly in the case of high fructose or sucrose intake. OXY was suggested as a contributor to the hypoglycemic effect of TW and RB. Further studies are needed for the systemic effect of TW and RB in circulation.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.2
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• pp.194-199
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• 2011

This study was conducted to evaluate fertigation effects of pig slurry (PS) and chemical fertilizer (CF) in cucumber by investigating the growth and yield, nutrient content and uptake, nutrient use efficiency, and soil characteristics in greenhouse cultivation. The cropping patterns of cucumber were semi-forcing culture and retarding culture, and the experiment involves four treatments: No fertilizer (NF), $26mg\;L^{-1}$ and $52mg\;L^{-1}$ of N application by slurry composting biofiltration liquefied fertilizer (SCBLF), and $52mg\;L^{-1}$ of N treatment by chemical fertilizer. The difference on the plant height of cucumber between SCBLF and CF treatments was no significant, but fresh weight and dry weight of stem and root were higher in $26mg\;L^{-1}$ SCBLF treatment. The Ca content of the leaf was lower in the treatments of SCBLF and the K content of the root was lower in the $52mg\;L^{-1}$ CF treatment. The Ca content of the stem was lowest in the $52mg\;L^{-1}$ CF treatment and the mineral content of the fruit showed no significant difference. In case of semi-forcing culture, the gross yield was lower in the $52mg\;L^{-1}$ CF treatment and the yield of unmarketable fruit was lower in the SCBLF treatments. The nutrient use efficiency of cucumber is as follows: K (8.3 ~ 30.9%), N (4.2 ~ 13.0%), P (1.9 ~ 2.0%). The SCBLF treatments showed higher figure in the soil pH than that of the CF treatment, while EC and the content of $NO_3$-N were higher in the CF treatment than the former. The content of exchangeable K was higher in the plot treated with $52mg\;L^{-1}$ SCBLF, and there were no significant differences in the content of Ca and Mg between the treatments. In conclusion, it is suggested that the application of liquefied manure made from pig slurry may be able to replace the use of chemical fertilizer in nitrogen and potassium.

• Journal of Korean Society of Forest Science
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• v.112 no.4
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• pp.502-514
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• 2023

This study was conducted to understand the long-term changes in soil physiochemical properties and seedling growth in Larix kaempferi (larch) stands planted in clear-cut larch and Pinus rigida (pine) forest soils over an 11-year period after reforestation. Two-year-old bare-root larch seedlings were planted in 2009-2010 at a density of 3,000 seedlings ha^-1 in clear-cut areas that harvested larch (Chuncheon and Gimcheon) and pine (Wonju and Gapyeong) stands. We analyzed the physiochemical properties of the mineral soils sampled at 0-20 cm soil depths in the planting year, and the 3^rd, 7^thand 11^th years after planting, and we measured seedling height and root collar diameter in those years. We found significant differences in soil silt and clay content, total carbon and nitrogen concentration, available phosphorus, and cation exchangeable capacity between the two stands; however, seedling growth did not differ. The mineral soil was more fertile in Gimcheon than in the other plantations, while early seedling growth was greatest in Gapyeong. The seedling height and diameter at 11 years after planting were largest in Wonju (1,028 tree ha^-1) and Chuncheon (1,359 tree ha^-1) due to decreases in stand density after tending the young trees. The soil properties in all plantations were similar 11 years after larch planting. In particular, the high sand content and high available phosphorus levels (caused by soil disturbance during clear-cutting and planting) showed marked decreases, potentially due to soil organic matter input and nutrient uptake, respectively. Thus, early reforestation after clear-cutting could limit nutrient leaching and contribute to soil stabilization. These results provide useful information for nutrient management of larch plantations.

• Korean Journal of Environmental Agriculture
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• v.25 no.3
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• pp.217-227
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• 2006

Micronutrient status in soils and crops of plastic film house and their relationship were investigated. Total 203 plastic film houses were selected (red pepper, 66; cucumber, 63; tomato, 74) in Yeongnam region and soil and leaf samples were collected. Hot-water extractable B and 0.1 N HCl extractable Cu, Zn, Fe, and Mn in soil samples and total micronutrients in leaf samples were analyzed. Contents Zn, Fe, and Mn in most of the investigated soils were higher than the upper limits of optimum level for general crop cultivation. Contents of Cu in most soils of cucumber and tomato cultivation were higher than the upper limit of optimum level, but Cu contents in about 30% of red pepper cultivation soils were below the sufficient level. Contents of B in most soils of cucumber and tomato were above the sufficient level but in 48% of red pepper cultivation soils B were found to be deficient. Micronutrient contents in leaf of investigated crops were much variable. Contents of B, Fe, and Mn were mostly within the sufficient levels, while in 71% of red pepper samples Cu was under deficient level and in 44% of cucumber samples Cu contents were higher than the upper limit of sufficient level. Contents of Zn in red pepper and cucumber samples were mostly within the sufficient level but in 62% of tomato samples Zn contents were under deficient condition. However, any visible deficiency or toxicity symptoms of micronutrients were not found in the crops. No consistent relationships were found between micronutrient contents in soil and leaf, and this indicates that growth and absorption activity of root and interactions among the nutrients in soil might be important factors in overall micronutrient uptake of crops. For best management of micronutrients in plastic film house, much attention should be focused on the management of soil and plant characteristics which control the micronutrient uptake of crops.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.39-46
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• 2015

Objective of this research was to investigate the influence of compositions and concentrations of fertilizer solutions on the vegetative growth and nutrient uptake of 'Seolhyang' strawberry. To achieve this, the solutions of acid fertilizer (AF), neutral fertilizer (NF), and basic fertilizer (BF) were prepared at concentrations of 100 or $200mg{\cdot}L^{-1}$ based on N and applied during the 100 days after transplanting. The changes in chemical properties of the soil solution were analysed every two weeks, and crop growth measurements as well as tissue analyses for mineral contents were conducted 100 days after fertilization. The growth was the highest in the treatments with BF, followed by those with NF and AF. The heaviest fresh and dry weights among treatments were 151.3 and 37.8 g, respectively, with BF $200mg{\cdot}L^{-1}$. In terms of tissue nutrient contents, the highest N, P and Na contents, of 3.08, 0.54, and 0.10%, respectively, were observed in the treatment with NF $200mg{\cdot}L^{-1}$. The highest K content was 2.83%, in the treatment with AF $200mg{\cdot}L^{-1}$, while the highest Ca and Mg were 0.98 and 0.42%, respectively, in BF $100mg{\cdot}L^{-1}$. The AF treatments had higher tissue Fe, Mn, Zn, and Cu contents compared to those of NF or BF when fertilizer concentrations were controlled to equal. During the 100 days after fertilization, the highest and lowest pH in soil solution of root media among all treatments tested were 6.67 in BF $100mg{\cdot}L^{-1}$ and 4.69 in AF $200mg{\cdot}L^{-1}$, respectively. The highest and lowest ECs were $5.132dS{\cdot}m^{-1}$ in BF $200mg{\cdot}L^{-1}$ and $1.448dS{\cdot}m^{-1}$ in BF $100mg{\cdot}L^{-1}$, respectively. For the concentrations of macronutrients in the soil solution of root media, the AF $200mg{\cdot}L^{-1}$ treatment gave the highest $NH_4$ concentrations followed by NF $200mg{\cdot}L^{-1}$ and AF $100mg{\cdot}L^{-1}$. The K concentrations in all treatments rose gradually after day 42 in all treatments. When fertilizer concentrations were controlled to equal, the highest Ca and Mg concentrations were observed in AF followed by NF and BF until day 84 in fertilization. The BF treatments produced the highest $NO_3$ concentrations, followed by NF and AF. The trends in the change of $PO_4$ concentration were similar in all treatments. The $SO_4$ concentrations were higher in treatments with AF than those with NF or BF until day 70 in fertilization. These results indicate that compositions of fertilizer solution should to be modified to contain more alkali nutrients when 'Seolhyang' strawberry is cultivated through inert media and nutri-culture systems.

• Korean Journal of Environmental Agriculture
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• v.32 no.4
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• pp.332-337
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• 2013

BACKGROUND: Newly reclaimed land has poor soil environment for crop growth since it is high in salt concentration but low in organic content compared with ordinary soil. It is known that whole-crop-barley can grow better in the soil of relatively high salt concentration than other crops but, the growth is poor at the concentration if higher than certain amount and it is a difficulty to secure productivity. Hence, the level of soil salt concentration suitable for the production of bulky feed in newly reclaimed land has been investigated. METHODS AND RESULTS: At Saemanguem reclaimed land, the land for the soil salt concentration electrical conductivity (EC) 0.8, 3.1, 6.5, 11.0 dS/m was selected; and chemical fertilizer $N-P_2O_5-K_2O$ (150-100-100kg/ha) was tested; and forage barley 220kg/ha were sown. The soil salt concentration during the cultivation period decreased in the order of harvest season>earing season>sowing season>wintering season, and the salt concentration in harvest season is 1.4-4.2 times higher than that of the sowing season. The higher the salt concentration, the poorer the over ground growth due to poor rooting; especially at EC 11.0 ds/m there was emergence but, it blighted after wintering. The Yield from the soil salt concentration 3.1dS/m and 6.5 dS/m was 68% and 35% from that of the soil salt concentration 0.8 dS/m (8.8 MT/ha) respectively. The proline content in early life stage was more than that of the harvest season, and it increased with salt concentration. The higher salt concentration, the more $Na_2O$ and MgO content in harvest season; but the higher the salt concentration, the less the content of N, $P_2O_5$, $K_2O$ and CaO. CONCLUSION(S): When the soil salt concentration becomes higher than 3.1 dS/m, the yield becomes poor because there is serious growth inhibition of forage barley both in root part and above aerial part that results in unbalanced absorption of nutrients. Therefore, it is recommended that the salt concentration should be lowered below 3.1 dS/m by underground drainage facilities or irrigating water for the stable production of whole-crop-barley.

• Korean Journal of Environmental Agriculture
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• v.32 no.1
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• pp.1-8
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• 2013

BACKGROUND: Many studies associated with cadmium (Cd) immobilization using lime fertilizer have been conducted for several decades. However, these studies did not suggest exact mechanism of Cd immobilization using lime fertilizer and evaluated effect of lime fertilizer on Cd phytoavailability in rice paddy soil under field condition. METHODS AND RESULTS: This study was conducted to determine exact mechanism of Cd immobilization using lime fertilizer and evaluate liming effect on Cd uptake of rice in contaminated paddy soil. $Ca(OH)_2$ was mixed with Cd contaminated arable soil at rates corresponding to 0, 1,000, 2,000, 4,000, and 8,000 mg/kg. The limed soil was moistened to paddy soil condition, and incubated at $25^{\circ}C$ for 4 weeks. $NH_4OAc$ extractable Cd concentration in soil decreased significantly with increasing $Ca(OH)_2$ rate, since $Ca(OH)_2$ markedly increased net negative charge of soil by pH increase, and decreased bioavailable Cd fractions (F1; exchangeable + acidic and reducible Cd fraction). Calculated solubility diagram indicated that Cd solubility was controlled by soil-Cd. $NH_4OAc$ extractable Cd and F1 concentration were negatively related to soil pH and negative charge. $Ca(OH)_2$ was applied at rates 0, 2, 4, and 8 Mg/ha and then cultivated rice in the paddy soil under field condition. Cadmium concentrations in grain, straw, and root of rice plant decreased significantly with increasing application rate of $Ca(OH)_2$. CONCLUSION(S): Alleviation of Cd phytoavailability with $Ca(OH)_2$ can be attributed primarily to Cd immobilization due to the increase in soil pH and negative charge rather than precipitation of $Cd(OH)_2$ or $CdCO_3$, and therefore, $Ca(OH)_2$ is effective for reducing Cd phytoavailability of rice in paddy soil.

• Korean Journal of Environmental Agriculture
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• v.18 no.1
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• pp.77-82
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• 1999

Three floating islands have been constructed for water quality improvement for a polluted irrigation reservoir. Each floating island consists of 10 segments. Each segment hay an area of $16m^2$(4×4m) and is made of wood frames and floats(polystyrene foam). We planted three species of aquatic macrophytes(Typha angustifolia, Zizania latifolia, and Phragmites australis) in floating island on June, 1998. They grew very well without death. We would like to evaluate Phragmites australis is the most suitable aquatic macrophyte that could be planted in a floating island because it maintained the best balance of its root and shoot among them. During their grown period, net primary productivity of Typha angustifolia was $962gDM/m^2$, Zizania latifolia was $1,115gDM/m^2$, and Phragmites australis was $523gDM/m^2$. From these data, it would be estimated to 5.0Kg uptake of nitrogen by aquatic macrophytes and phosphorus 0.8Kg in 3 floating islands. The floating islands worked well as a habitat of fish and prawns. Many kinds of insect lived on the floating islands. The floating island has not only the function of water quality treatment but also several advantages: improvement of landscape and species diversity; low cost of maintenance; low technology; unnecessary of energy; less susceptible to variations in pollutant loading. It could be evaluated a good measure of water quality improvement for an irrigation reservoir. However, it should be intensively studied to develop more light, strong, durable and low-priced frames for efficient floating islands.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.4
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• pp.287-292
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• 1990

A study has been conducted since 1954 to elucidate the effects of longterm application of ammonium sulfate (AS), urea, compost, and calcium silicate(CS) fertilizers on major soil nutrients and rice yields in wetland soil. The soil pH in the AS plot become lower than that in the urea plot but had little influence on rice yield. Continuous application of AS and urea with compost and CS raised the soil pH compared with single applications of AS or urea. Soil organic matter content increased yearly in plot with no fertilizer and in those with AS and urea, and increased considerably in plots with compost and CS. Application of compost and CS promoted rice top growth as well as root growth, resulting in increased dry matter. Soil contents of N, $P_2O_5$, Ca, Mg, and $SiO_2$ in the NPK with composts plot increased considerably compared with the NPK plot; however. the exchangeable K content was rather low. Rice growth increased with compost application. which enhanced K uptake, and the coarse texture promoted K leaching. With compost and CS addition, con tents of exchangeable Ca and Mg in the AS plot decreased considerably more than in the urea plot. Probably this was the result of higher Ca and Mg precipitation by sulfate in the AS plot. The re are no significant differences in rice yield between AS and urea plots. With compost and CS treatments, rice yields increased in the AS plot compared with the urea plot. The increase in yield is considered to be the effect of S. which is a constituent of AS.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.4
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• pp.649-656
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• 2012

To obtain the basic information for agricultural utilization of germanium (Ge), the growth characteristics and Ge absorption in rice plant were investigated under different Ge concentrations by foliar spray application. The Ge concentrations were treated with 0 (control), 10, 20, 40 and $80mg\;L^{-1}$ in pot (1 5000$^{-1}$ a), respectively. The Ge absorption rate in rice by foliar spray application with $80mg\;L^{-1}$ in pot was higher in the order of leaf (5.75%) > stem (4.52%) > root (<0.01%). By foliar spray application, the Ge content in rice was higher in the order of $80mg\;L^{-1}$ > $40mg\;L^{-1}$ > $20mg\;L^{-1}$ > $10mg\;L^{-1}$. When rice was treated with $80mg\;L^{-1}$ of Ge, the Ge content in rice grain was higher in the order of rice bran ($0.21mg\;pot^{-1}$) $\gg$ brown rice ($0.04mg\;pot^{-1}$) ${\geq}$ polished rice ($0.03mg\;pot^{-1}$). By foliar spray application, the Ge uptake in rice bran was higher than that in other parts. Therefore, optimum Ge concentration by foliar spray application was $80mg\;L^{-1}$ in pot based on the results from the Ge treatments.