• Title/Summary/Keyword: Root numbers

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Effects of Auxins and Cytokinins on Organogenesis of Soybean Glycine max L.

  • Kim, Kyong-Ho;Park, Ho-Ki;Park, Moon-Soo;Yeo, Up-Dong
    • Journal of Plant Biotechnology
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    • v.3 no.2
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    • pp.95-100
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    • 2001
  • To select the section with shoot formation ability, the calli and shoot formation from three sections (first leaf including cotyledonary node, hypocotyl and cotyledon explants) of 5-days-seedlings of soybean were induced on MS medium supplemented with 1.0 mg/L BAP, 3% sucrose, and 0.3% gelrite for one month. The first leaf section exhibited the highest shoot formation rate (51%), followed the hypocotyl section (10%) and the cotyledon section (0%). The shoot formation rates and shoot number of the four excised sections (whole first leaf, a half of the first leaf, a third of the first leaf and only node) of the first leaf were also investigated on the same medium. A half of the first leaf explant and the third of the first leaf explant had higher shoot formation rates (76-80%) and numbers (3-4 / explants) than those in other two explants. Effects of six cytokinins (kinetin, zeatin, BAP, 2iP, PBA, and TDZ) on shoot formation were determined, using the half of the first leaf explants. Zeatin (1.0 mg/L) exhibited the highest in shoot formation rate (94%) and numbers (8 / explant). In addition, the combined effects of three cytokinins (zeatin, BAP, and TDZ; 0.5, 1.0, 2.0 mg/L, respectively) and an auxin (IAA; 0.0, 0.5, 1.0, 2.0 mg/L) were determined. The combination (1:1, v/v) of zeatin (1.0 mg/L) and IAA (1.0 mg/L) exhibited the highest in shoot formation rate (96%) and numbers (16 / explant), twice more than zeatin (1.0 mg/L) alone. The shoot cuttings were transferred and cultivated on the rooting media supplemented with only auxin, IBA at various concentrations. The highest root formation (8 / shoot) was achieved on the medium supplemented with 1.5 mg/L. After 4 weeks of cultivation, the plantlets with an extensive root system were transplanted in pots with a soil mixture of vermiculite and fine sand. Transferred to field, about 75% of the plantlets survived.

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Effect of Nutrient Concentration and Plant Growth Regulators on Rooting in Coleus Stem Cuttings (코레우스의 삽목시 배양액과 생장조절제처리가 발근에 미치는 영향)

  • 조은희;장매희
    • Journal of Bio-Environment Control
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    • v.6 no.4
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    • pp.277-283
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    • 1997
  • The effect of nutrient concentration and plant growth regulators on rooting of Coleus stem cuttings were investigated. In contrast to sand cutting, the nutrient solution or plant growth regulator treatment increased the root numbers and root length. The concentration of nutrient solution were 1, l/2 and 1/4 strength of the balanced nutrient solution developed by Japanese Horticultural Experimental Station and the highest root growth was obtained from 1/4 strength nutrient concentration. NAA or IBA treatment in culture solution stimulated the rooting and 0.01mg/l of NAA or IBA was effective in root development. Furthermore, days for rooting were shortened and root growth were promoted by mixing nutrient solutions with plant growth regulators and by using cutting with leaves. The rooting of Coleus was observed under microscope and the adventitious root formation initiated at 3 days after cutting with nutrient and auxin mixture.

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Endophytic Bacteria Improve Root Traits, Biomass and Yield of Helianthus tuberosus L. under Normal and Deficit Water Conditions

  • Namwongsa, Junthima;Jogloy, Sanun;Vorasoot, Nimitr;Boonlue, Sophon;Riddech, Nuntavan;Mongkolthanaruk, Wiyada
    • Journal of Microbiology and Biotechnology
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    • v.29 no.11
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    • pp.1777-1789
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    • 2019
  • Drought is more concerned to be a huge problem for agriculture as it affects plant growth and yield. Endophytic bacteria act as plant growth promoting bacteria that have roles for improving plant growth under stress conditions. The properties of four strains of endophytic bacteria were determined under water deficit medium with 20% polyethylene glycol. Bacillus aquimaris strain 3.13 showed high 1-aminocyclopropane-1-carboxylate (ACC) deaminase production; Micrococcus luteus strain 4.43 produced indole acetic acid (IAA). Exopolysaccharide production was high in Bacillus methylotrophicus strain 5.18 while Bacillus sp. strain 5.2 did not show major properties for drought response. Inoculation of endophytic bacteria into plants, strain 3.13 and 4.43 increased height, shoot and root weight, root length, root diameter, root volume, root area and root surface of Jerusalem artichoke grown under water limitation, clearly shown in water supply at 1/3 of available water. These increases were caused by bacteria ACC deaminase and IAA production; moreover, strain 4.43 boosted leaf area and chlorophyll levels, leading to increased photosynthesis under drought at 60 days of planting. The harvest index was high in the treatment with strain 4.43 and 3.13 under 1/3 of available water, promoting tuber numbers and tuber weight. Inulin content was unchanged in the control between well-watered and drought conditions. In comparison, inulin levels were higher in the endophytic bacteria treatment under both conditions, although yields dipped under drought. Thus, the endophytic bacteria promoted in plant growth and yield under drought; they had outstanding function in the enhancement of inulin content under well-watered condition.

An Evaluation of Tree Roots Effect on Soil Reinforcement by Direct Shear Test (일면전단실험에 의한 수목뿌리의 토양보강효과 평가)

  • Cha, Du Song;Oh, Jae-Heun
    • Journal of Korean Society of Forest Science
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    • v.94 no.4 s.161
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    • pp.281-286
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    • 2005
  • Trees enhance slope stability against down slope mass movement through the removal of soil water by transpiration and by the mechanical reinforcement of their roots. To assess the magnitude of this reinforcement on natural slope stability, direct shear tests were made on dry sand reinforced with different array types of roots. Pinus koraiensis was used as root specimens. The peak shear resistance at each normal stress level was measured on the rooted and unrooted soil specimens. Increased soil resistance(${\Delta}S$) by roots was calculated using parameters like internal friction angle and cohesion of tested soil and also evaluated the effects of root array in tested soil. As results, we find that shear resistance increased in tested soil shear box as diameters and arrayed numbers of root specimen increased and cross root array in tested soil had a much greater reinforcing effect than other root arrays. Comparison of traditional root-soil model with experiments showed that simulated reinforce strength by the model was different with those obtained by the experiment due to its linearity.

The Effect of EDTA, Tetracycline-HCl, and Citric Acid on Diseased Root Surfaces; The SEM Study (EDTA, 염산 테트라싸이클린, 구연산 처치가 치근면에 미치는 영향)

  • Ahn, Seong-Hee;Chai, Jung-Kiu;Kim, Chong-Kwan;Cho, Kyoo-Sung
    • Journal of Periodontal and Implant Science
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    • v.29 no.3
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    • pp.561-578
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    • 1999
  • The goal of periodontal therapy is the periodontal regeneration by the removal of microorganisms and their toxic products from the periodontally diseased root surface. To achieve periodontal regeneration, root conditioning as an adjunct to root planing has been done. There are low pH etchants such as citric acid, tetracycline-HCl, and EDTA solution which is a neutral chelating agent. The purpose of present study was to examine the effect of root conditioning by citric acid, tetracycline HCl, and EDTA. Total 35 root specimens(6${\times}$3${\times}$2mm) were prepared from the periodontally diseased teeth, scaled and root planed. The specimens were treated with normal saline for 1 minute, saturated citric acid(pH 1) for 3 minutes, 50mg/ml tetracycline-HCl(pH 2) for 5 minutes, 15% EDTA(pH 7) for 5 minutes using rubbing technique. The specimens were examined under scanning electron microscopy at 1000, and 3000 magnification. On the microphotographs taken at 1000 magnification, the numbers of opened and patent dentinal tubules per unit area(10,640${\mu}m^2$) were counted. And the diameters of opened dentinal tubules per unit are (10,640${\mu}m^2$) were measured. The differences of number and diameter among all groups were statistically analyzed by Kruskal Wallis Test. The results were as follows; 1. In the specimens applied with normal saline(control group), the root surface was finely cracked, and was covered by irregular smear layer. Neither exposed dentinal tubules nor any patent dentinal tubules could be seen. 2. In the specimens applied with saturated citric acid(experimental 1 group), the globular collagen fibers were exposed around the peritubular space, and many dentinal tubules were revealed. 3. In the specimens applied with tetracycline-HCl(experimental 2 group), the process-like collagen fibers were exposed around the peritubular space, and some dentinal tubules were revealed. 4. In the specimens applied with 15% EDTA(experimental 3 group), the root surface was covered by the collagenous fibrillar network, and many dentinal tubules were revealed. 5. The numbers of opened and patent dentinal tubules were significantly more in exp. 1 group and exp. 3 group than in exp. 2 group(P<0.05). But there was no significant difference between exp. 1 group and exp. 3 group. In control group, the number of opened and patent dentinal tubules could not be counted because any dentinal tubules couldn't be seen. 6 . The diameter of opened dentinal tubules was significantly smaller in exp. 1 group and exp. 3 group than in exp. 2 group(P<0.05). But there was no significant difference between exp. 1 group and exp. 3 group. In control group, the diameter of opened dentinal tubules could not be measured because any dentinal tubules couldn't be seen. The results demonstrate that root conditioning with citric acid, tetracycline- HCl, and EDTA is more effective in periodontal healing than only root planing, and 15% EDTA solution can replace low pH etching agents such as citric acid, tetracycline-HCl for root conditioning.

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Analyzing Errors of Mathematics Under-Achievers in Understanding the Concept of the Square Root of Positive Numbers and Related Calculations (제곱근의 뜻과 성질에 대한 이해 및 근호를 포함한 식의 계산에서 나타나는 수학 학습 부진 학생들의 오류 분석)

  • Do, Jong Hoon;Kwon, Oh Byeong
    • Journal of the Korean School Mathematics Society
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    • v.22 no.1
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    • pp.1-21
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    • 2019
  • Mathematics is one of the subjects in which learners seriously experience under-achievements in school education. Middle school level mathematics especially plays such a role as a bridge between elementary level informal mathematics and high school level formal mathematics that learners' under-achievements in the middle school level mathematics may yield more serious under-achievements later. Therefore it is crucial to prevent learners' later under-achievements that we analyze the status of under achievements including analysing various under-achievers' errors in the middle school level mathematics. From this point of view, we analysed errors of mathematics under-achievers in understanding the concept of the square root of positive numbers and related calculations in this paper. As the results of our research, we found some unexpected errors of 'some mathematics under-achievers regarding the mathematical symbol ${\surd}$ of square root as a parenthesis ( ), and others interpret $x=-2{\pm}{\sqrt{10}}$ as x=-2 or ${\pm}{\sqrt{10}}$.' that suggest the necessity of more various and in-depth discussions and researches of analysis on learners' errors and misconceptions in all areas of school mathematics.

Comparisons of orthodontic root resorption under heavy and jiggling reciprocating forces during experimental tooth movement in a rat model

  • Hikida, Takuji;Yamaguchi, Masaru;Shimizu, Mami;Kikuta, Jun;Yoshino, Tomokazu;Kasai, Kazutaka
    • The korean journal of orthodontics
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    • v.46 no.4
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    • pp.228-241
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    • 2016
  • Objective: Root mobility due to reciprocating movement of the tooth (jiggling) may exacerbate orthodontic root resorption (ORR). "Jiggling" describes mesiodistal or buccolingual movement of the roots of the teeth during orthodontic treatment. In the present study, buccolingual movement is described as "jiggling." We aimed to investigate the relationship between ORR and jiggling and to test for positive cell expression in odontoclasts in resorbed roots during experimental tooth movement (jiggling) in vivo. Methods: Male Wistar rats were divided into control, heavy force (HF), optimal force (OF), and jiggling force (JF) groups. The expression levels of cathepsin K, matrix metalloproteinase (MMP)-9 protein, interleukin (IL)-6, cytokine-induced neutrophil chemoattractant 1 (CINC-1; an IL-8-related protein in rodents), receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL), and osteoprotegerin protein in the dental root were determined using immunohistochemistry. Results: On day 21, a greater number of root resorption lacunae, which contained multinucleated odontoclasts, were observed in the palatal roots of rats in the JF group than in rats from other groups. Furthermore, there was a significant increase in the numbers of cathepsin K-positive and MMP-9-positive odontoclasts in the JF group on day 21. Immunoreactivities for IL-6, CINC-1, and RANKL were stronger in resorbed roots exposed to jiggling than in the other groups on day 21. Negative reactivity was observed in the controls. Conclusions: These results suggest that jiggling may induce ORR via inflammatory cytokine production during orthodontic tooth movement, and that jiggling may be a risk factor for ORR.

A Roentgenographic Study on the Development of Roots of Mandibular Permanent Posterior Teeth (하악영구구치 치근발육에 관한 방사선학적 연구)

  • 고명연;정성창
    • Journal of Oral Medicine and Pain
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    • v.6 no.1
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    • pp.23-34
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    • 1981
  • In order to evaluate the correlation of age with development stage on permanent lower posterior teeth. the author exmined the roentgeregrams in standard films taken by intraoral technic and analysed the development phases of 1358 teeth of 500 males ranging from 9 to 15 years. The development was divided into 7 phases : Crown complete (Cr. C.). Root length 1/4(R. 1/4) Root length /2 (R. 1/2) Apical closure complete (A.C) The obtained results were as follows : 1. The formation of roots in full length on posterior teeth was complete as follow : a. Roots of 1st premolar : 12.72 years b. Roots of 2nd premolar : 12.94 years c. Meral Roots of 2nd molar : 13.38 years d. Distal Roots of 2nd molar : 13.46 years 2. The formation of apical forman of premolar was closured as follows : a. Apical foramen of root of 1st premolar : 13.64 years b. Apical foramen of root of 2nd premolar : 13.93 years 3. As a general rule. the mesial roots of second molar were developed earlier than distal roots of second molar. 4. In the correlation of age with the development stage, the regression equations. the correlation coefficents. and the sample numbers were “Y = 0.8370x + 10.2160, r = 0.71(p<0.01), n = 318”on lower first premolar, “Y = 0.6984x + 10.2148, r = 0.71(p<0.01), n = 385”on lower second premolar, “Y = 0.8810x + 10.2040, r = 0.65(p<0.01), n = 344”on mesial Root of lower second molar, and “Y = 0.7310x + 10.7940, r = 0.66(p<0.01), n = 311”on Distal Root of lower second molar respectively.

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Relationship between Vertical Root Distribution and Yield Traits in IRRI's New Plant Type Rice

  • Kim, Bo-Kyeong;Kang, Si-Yong;Shin, Hyun-tak;Yang, Sae-Jun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.1
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    • pp.20-25
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    • 1999
  • This experiment was conducted to elucidate the relation-ship between vertical distribution of rice roots and yield traits under field conditions. Eight IRRI's new plant type rices (NPTRs) were tested in a volcanic ash soil paddy field under dense (IO 10 cm) and common (20 20 cm) planting densities. These lines were evaluated to have more spikelet numbers per panicle (SNP), lower filled grain rate (FGR), and lower rough grain weight per hill (RGWH). In dense planting, rough grain weight per stem (RGWS) was increased due to heavier culm and leaf dry weight (CLDW), and both RGWS and CLDW were related with the percentage of root distribution (%RWI) in the 10~30 cm soil layer, while in common planting, RGWS was not closely related with CLDW. SNP was highly related with root dry weight (RDW) in the 0~10cm soil layer. FGR was mainly affected by ROW in the 10~30 cm soil layer under both planting densities. RGWS was positively correlated with top dry weight (TDW) and harvest index (HI), and TDW was positively correlated with RWI under common planting or %RWI under dense planting, and HI was positively correlated with RWI in the 10~30 cm soil layer only under dense planting. RGWS was closely related with root weight index by dry weight (RWI) in the 10~30 cm soil layer and %RWI in the 0~30 cm or 10~30 cm soil layer under dense planting, and with only RWI in the 10~30 cm soil layer under common planting. But RGWH showed the close positive relationship with RDW and RWI in the 10~30 cm soil layer under dense planting, while under common planting, it showed the close positive relationship with RWI and %RWI in the 10~30 cm soil layer or %RWI in the 0~30 cm soil layer. The deeper root system in rice, especially under dense planting, is important for high yield of NPTRs focusing on the increment of top mass production and harvest index.

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ABOUT THE PERIOD OF BELL NUMBERS MODULO A PRIME

  • Car, Mireille;Gallardo, Luis H.;Rahavandrainy, Olivier;Vaserstein, Leonid N.
    • Bulletin of the Korean Mathematical Society
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    • v.45 no.1
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    • pp.143-155
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    • 2008
  • Let p be a prime number. It is known that the order o(r) of a root r of the irreducible polynomial $x^p-x-l$ over $\mathbb{F}_p$ divides $g(p)=\frac{p^p-1}{p-1}$. Samuel Wagstaff recently conjectured that o(r) = g(p) for any prime p. The main object of the paper is to give some subsets S of {1,...,g(p)} that do not contain o(r).