• Title/Summary/Keyword: Root initiation

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Effect of Carbenicillin on Callus Induction and Regeneration Efficiency of Tissues of Horseradish(Armoracia rusticana)

  • Bae, Chang-Hyu
    • Plant Resources
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    • v.4 no.1
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    • pp.53-58
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    • 2001
  • The effect of carbenicillin on the dedifferentiation and the regeneration efficiency of plant tissues of horseradish(Armoracia rusticana) was evaluated, Inhibition effect for callus initiation was observed when leaf blade, root and petiole segments were grown on MS medium containing 500 mg/L to 2000 mg/L carbenicillin and 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The regeneration of horseradish shoots from leaf blade, root and petiole explants were decreased as the addition of carbenicillin increased from 1000 mg/L to 2000 mg/L in MS medium containing 0.5 mg/L of 6-benzylaminopurine (BAP) or kinetin. Especially, 500 mg/L carbenicillin treatment significantly inhibited shoot induction when leaf blade explants were grown on hormone-free MS medium. It was suggested that the toxic effects of combinations of carbenicillin and 2,4-D may be due to high auxin activity levels.

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Fatigue Assessment of Load-carrying Asymmetric Double Bevel Cruciform Welded Joints using Structural Stress Approach (구조응력을 이용한 하중 전달형 십자 양면 비대칭 필렛 용접 시험편의 피로강도 평가)

  • Kim, Seong-Min;Kim, Young-Nam;Lee, Seung-Hyun;Kim, Myung-Hyun
    • Journal of Welding and Joining
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    • v.30 no.6
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    • pp.86-91
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    • 2012
  • Fatigue tests and analyses were carried out to investigate fatigue strength and crack initiation point of load-carrying asymmetric double bevel cruciform welded joints. Mesh-insensitive structural stress approach was adopted to estimate high precise fatigue life and crack initiation point. Two different case material and weld shape were considered in this study. Results of fatigue tests and analyses were compared and discussed in consideration of applicability of structural stress approach as the reliable fatigue assessment method of cruciform welded joints.

Investigation of the Contributions of Creep and Thermal Fatigue to Failure of a High-Intermediate Pressure Steam Turbine Casing

  • Lee, Jaehong;Jung, Nam-gun
    • KEPCO Journal on Electric Power and Energy
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    • v.6 no.1
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    • pp.41-47
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    • 2020
  • The contribution of damage mechanisms to failure of steam turbine casing made of Cr-Mo-V steel was investigated. Creep-fatigue interaction on the HP side corner of turbine casing was revealed as the root cause of the catastrophic failure performed by metallurgical analysis. The steady-state pressure and transient thermal stress were analyzed based on the actual operating condition of the thermal plant. Damage of creep-fatigue interaction to crack initiation was evaluated with multiaxial effects. The contribution ratio of creep and fatigue to the crack initiation was estimated to 3:1. Temporary geometrical correct action with repair weld was executed. For long-term operation, design improvement of casing equipment for creep resistance should be needed.

Growth and Chlorophyll Biosynthesis of Vigna angularis under Lead Stress

  • Suh-Young Koo;Sun
    • Journal of Environmental Science International
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    • v.1 no.2
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    • pp.145-155
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    • 1992
  • The effect of various supplies of lead singly and in combination with aluminium on growth and chlorophyll biosynthesis was investigated in 7-day-old Vigna anguluris seedlings. Expose to 50 $\mu$N Pb or more drastically reduced root elongation rate. Significant depressions in root growth was observed within 1 day and no recovery of growth was seen over the duration of treatment period. Root elongation decreased depending on the Pb concentrations. Root growth inhibition was stronger than shoot growth inhibition. The initiation of lateral roots appeared to be more sensitive to Pb than the growth of main roots. Inhibition of root and shoot elongation by Pb was lessened by combined exposure of Pb and Al, suggesting that the presence of AA reverse the inhibitory effect of Pb alone. With the histochemical sodium rhodizonate method the rate of Pb uptake was dependent on the Pb concentration and exposure time of the roots to Pb salts. Pb was first deposited on the root surface and then translocated radially in the root cap cells. During a longer Pb administration (up to 72 h) Pb penetration was nonuniform, with accumulation within the cortex or endodermis. There was drastic reduction in chlorophyll content by Pb. The Pb inhibition of chlorophyll synthesis was concentration dependent. 5-Aminolevulinic acid dehydratase (ALAD) activity exhibited distinct inhibition from control. Reduction in chlorophyll content was accompanied by proportional changes in ALAD activity. Chlorophyll content and ALAD activity were less affected by combined exposure of Pb and Al, suggesting that Al has a protective effect against the inhibiting action of Pb on photosynthetic activity.

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Growth and Chlorophyiil Biosynthesis of Vigna angularis under Lead Stress

  • Koo Suh-Young;Jin Sun-Young;Hong Jung-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.1 no.2
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    • pp.145-155
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    • 1997
  • The effect of various supplies of lead singly and in combination with aluminium on growth and chlorophyll biosynthesis was investigated in 7-day-old Vigna angularis seedlings. Expose to 50 uM Pb or more drastically reduced root elongation rate. Significant depressions in root growth was observed within 1 day and no recovery of growth was seen over the duration of treatment period. Root elongation decreased depending on the Pb concentrations. Root growth inhibition was stronger than shoot growth inhibition. The initiation of lateral roots appeared to be more sensitive to Pb than the growth of main roots. Inhibition of root and shoot elongation by Pb was lessened by combined exposure of Pb and Al, suggesting that the presence of Al reverse the inhibitory effect of Pb alone. With the histochemical sodium rhodizonate method the rate of Pb uptake was dependent on the Pb concentration and exposure time of the roots to Pb salts. Pb was first deposited on the root surface and then translocated radially in the root cap cells. During a longer Pb administration (up to 72 h) Pb penetration was nonuniform, with accumulation within the cortex or endodermis. There was drastic reduction in chlorophyll content by Pb. The Pb inhibition of chlorophyll synthesis was concentration dependent. $\delta-Aminolevulinic$ acid dehydratase (ALAD) activity exhibited distinct inhibition from control. Reduction in chlorophyll content was accompanied by proportional changes in ALAD activity. Chlorophyll content and ALAD activity were less affected by combined exposure of Pb and Al, suggesting that Al has a protective effect against the inhibiting action of Pb on photosynthetic activity.

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In vitro Micropropagation of Rosa hybrid L.

  • Kim Chang-Kil;Oh Jung-Youl;Jee Sun-Ok;Chung Jae-Dong
    • Journal of Plant Biotechnology
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    • v.5 no.2
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    • pp.115-119
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    • 2003
  • To determine the appropriate concentrations of nutrients and growth regulators for shoot proliferation and root initiation, several rose hybrid tea cultivars were cultured. Cultured shoot tips and lateral buds from different cultivars proliferated multiple shoots on Murashige and Skoog (MS) medium supplemented with 0 to 4 mg/L BA and 0 to 0.05 mg/L NAA. The ability of the explants to proliferate shoots and initiate roots was affected by genotype, the nodal position of explant, the strength of MS basal medium and growth regulators used. The buds nearest the apex exhibited the slowest rate of development. Most cultivars had the highest shoot proliferation when cultured on MS medium with 2 mg/L BA and 0.01 mg/L NAA, but the degree varied by cultivars. Root development was enhanced by lowering the concentration of MS salts.

THE INFLUENCE OF pH ON THE FORMATION OF ARTIFICIAL ROOT CARIES IN ACID BUFFER SOLUTION (산 완충용액의 pH가 인공치근우식의 형성에 미치는 영향)

  • Oh, Hyun-Suk;Kum, Kee-Yeon;Ro, Byeng-Duck;Lee, Chan-Young
    • Restorative Dentistry and Endodontics
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    • v.24 no.3
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    • pp.495-502
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    • 1999
  • Recently root caries is reported as an increasing oral disease Many researchers studied in vivo and in vitro enamel lesions but due to its peculiar structure and different chemical composition compared to the enamel, little effort has been made to root caries study. The purpose of this study is to compare and to evaluate the effect of pH on the progression of artificial root caries lesion. Under the constant degree of saturation, the specimens were divided into 4 groups(pH 4.3, 5.0, 5.5, 6.0). Each group was immersed in acid buffer solution for 1, 3, 5, 7 days and examined using the polarizing microscope. The results were as follows: 1. The body of the lesion in the dentin showed higher degree of positive birefringence compared to sound dentin. 2. The rate of progression of the lesion slowed as the pH increased. 3. In all groups, the lesion progressed rapidly at the initiation or the experiment but increased gradually as time elapsed. In conclusion, the study shows that the pH in the acid buffer solution influenced the rate of progression of the lesion in artificial root caries. Compared to the previous enamel study, the initial pattern of the lesion progressed rapidly and as time elapsed increased gradually.

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Initiation and Propagation Behaviors of Micro-Surface-Fatigue Cracks under In-Plane Tension Fatigue Tests (引張 軸荷重 疲勞 에 의한 微小表面 균열 의 發생 . 成長擧動)

  • 서창민
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.9 no.1
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    • pp.1-9
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    • 1985
  • In-plane tension fatigue tests (R = 0.05) were carried out to investigate the initiation and propagation behaviors of micro-surface-fatigue cracks on smooth surfaces of a mild steel. Also, the investigations of saturated cyclic strain which can be obtained by the fatigue tests have been made via the cyclic strain intensity factor, .DELTA. $K_{\epsilon}$/, for the purpose of unifying two approaches of the study of fatigue; the one approach is based on the fracture mechanics concept and the other on lowcycle fatigue concept. Some of the results are as follows; The growth rate, d(2a)/dN, of small cracks cannot be represented by one straight line as a function of .DELTA.K for various of the nominal stress range, .DELTA..sigma., and is higher than that of a larger through crack. The rearrangement of the d(2a)/dN by .DELTA..epsilon..root..pi.s( = .DELTA. $K_{\epsilon}$/) with the stress range .DELTA..epsilon. in .DELTA.K replaced by .DELTA..epsilon., strain range, gives one straight line of the .DELTA. $K_{\epsilon}$-d(2a)/dN relation for various values of stress range .DELTA.$_{\epsilon}$../.X>../.

Relationship between cotyledon Number and Vascular System in Carrot Seedling (당근 유식물체에서 자엽수와 유관속계의 관련성)

  • 김경식
    • Korean Journal of Plant Resources
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    • v.11 no.1
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    • pp.30-39
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    • 1998
  • Carrot seedling emgryos showing variations in cotyledon number were selected and anatomical comparisons of the embryo vascular systems were performed between the variants and normal two cotyledonary (5) embryos from 800 seedings germinated . Externally, all of the four and six cotyledonary embryos had two cotyledonary petioles. Each of the cotyledonary petioles divided into two or three on the upper part fo the petiole which result in four and six cotyledons, respectively. However, the embryos had three different cotyledonary petioles in the three cotyledonary embryos. On the basis of the pattern of vascular system, the four and six cotyledonary embryos had the same basic vascular system as fnormal two cotyledonary embryos, Therefore the cotyledon number abnormality could result from the branching split of the abnormally thickened upper part of the cotyledonary petiole. However, the three cotyledonaryembryos had a different vascular system from the normla two cotyledonary embryos. They could be regarded as different varieties form the two cotyledon embryos. All embryos observed had short cylindrical plumule sheath which formed by the fusion of the cotyledon bases. The presence of plumule sheath strontgly implied that the initiation of the cotyledons was not from the two localized primordia but from the circular proimordiu formed at the blobular stageof embryo, and it is not consistent with current views of cotyledon initiation. On the formation of the primary vascular system of carrot seedlings, it is suggested that the primary vascular system of the plumule was formed independently from that of the root-hypocotyle-cotyledon system.

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An Experimental Study on Fatigue Life Evaluation of Welded Joints under Storm Loading (스톰 하중을 받는 용접 구조물의 피로 수명 평가에 대한 실험 연구)

  • Yoo, Chang-Hyuk;Kim, Kyung-Su;Suh, Yong-Suk;Shim, Yong-Lae;Ha, Yeong-Su;You, Won-Hyo;Choi, Hyun-Jun
    • Journal of the Society of Naval Architects of Korea
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    • v.49 no.1
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    • pp.99-108
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    • 2012
  • In this paper, fatigue tests are conducted for the specimens with longitudinal and transverse attachment under variable amplitude axial loading based on storm model. Considered loadings include repeated single storm, 6 or 8 storms randomly, and storms including calm sea condition while the mean stress and the maximum stress of loadings are changed. The effect of three variables are investigated; root mean square(RMS) value of stress amplitude, mean stress shift and maximum stress, which can characterize storm loading on fatigue life. In addition, experiments including calm sea loading are also carried out to investigate the effect of calm sea state. Test results are evaluated and compared with DNV-CN2005 and Matsuoka's method for the estimation of crack initiation and propagation life. To verify the validity of the criteria, the measured crack initiation lifes are compared with the specific crack length 15mm, which are calculated with beach marks.