• 한국약용작물학회지
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• 제15권1호
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• pp.46-50
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• 2007

하늘타리 (Trichosanthes kirilowii)로부터 조직배양 기법을 이용하여 약리작용을 나타내는 생리활성물질인 trichosantin을 생산하기 위한 연구로 Agrobacterium rhizogenes ATCC15834 의 감염을 통하여 기내 배양된 유묘의 잎절편으로부터 형질전환 모상근을 유도하였다. 유도된 모상근 clones으로부터 성장속도와 분지화 정도가 빠르며 단백질 합성능이 가장 우수한 TR-03 clones을 선발하였으며, 이를 사용하여 생체량과 배지내 가용성 단백질의 생산을 최대로 높일 수 있는 최적 배양조건을 조사하였다. TR-03 clone을 4% sucrose가 첨가된 MS 배지에 초기 접종농도 2 g (생중량)을 접종하여 100 rpm으로 배양시 생중량과 배지내 가용성 총단백질의 함량이 플라스크당 약 2.4 g (건중량)과 $28.3ug/\ell$으로 최대치를 나타내었다. 한편, 배지내 가용성 단백질에 대한 RIP활성 검정에서 배양 4주 후 21.3 unit로 최대치를 보였다.

• 생물환경조절학회지
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• 제29권4호
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• pp.365-372
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• 2020

본 연구는 원통형 종이포트 토마토 육묘시 Diniconazole의 처리방법이 도장억제 및 근권발달에 미치는 영향을 검토하기 위하여 수행되었다. 그 결과, 엽면적, LAR, 초장, 충실도, 생체중, RGR 및 R/S 에서 시험구간 유의한 차이를 보였다. 동일한 농도를 처리했을 경우, 근권부와 지상부의 흡수도 차이로 인해 저면관수가 엽면살포에 비해 도장억제에 효과적이었다. 저면관수는 엽면시비의 10분의 1의 농도만으로도, 20~30%정도의 동일한 도장억제 효과를 얻을 수 있었다. 디니코나졸 처리에의한 근권부 반응이 흥미로웠는데, 저면관수시 총근장, 근권부피, 평균 근경 및 근단수가 증가하였다. 특히, 0.3mm 이하의 초미세근이 감소하고 0.3~0.6mm의 세근이 증가하였다. 따라서 원통형 종이포트 육묘시 저면관수를 하는 것이 기존 엽면시비에 비해 사용량이 적으면서도 도장억제 및 근권부 활착률을 높힐 수 있을 것으로 판단된다.

• 생물환경조절학회지
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• 제12권4호
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• pp.207-216
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• 2003

고온기 분식 칼랑코에 재배에 있어 문제는 고온 스트레서에 의한 생장의 억제로, 본 연구는 지하부 및 지상부의 생장을 촉진을 시킬 수 있다고 알려진 근권 생장촉진미생물(Pseudomonas sp. B와 Pseudomonas sp. D2)을 선발하여 사용하였으며 천연물로서는 alginoligosaccharide와 glucosamine oligosaccharide를 사용하였다. 또한 이들의 AG-용액과 단일 및 복합 처리하여 재배 온도차이를 부여한 경우 칼랑코에의 생장에 미치는 영향을 조사하였다. 상이한 지하부 온도조건은 25$^{\circ}C$, 30$^{\circ}C$ 및 35$^{\circ}C$로 처리하여, 2주후 초장, 엽장,엽폭, 엽면적, 엽중, 지상부 생체중, 근장, 근중을 조사하였다. 25$^{\circ}C$처리구에서는 초장, 엽면적, 엽중, 근중 모두 Pseudomonas sp. B와 glucosamine oligosaccharide를 혼합한 처리구가 가장 높았고, 30$^{\circ}C$의 경우 초장과 엽중은 glusosamine oligosaccharide처리에서, 엽면적과 근중은 Pseudomonas sp. D2와 algin-oligosaccharide를 처리구에서 가장 좋은 생장 효과를 나타내었다. 35$^{\circ}C$의 경우는 초장, 엽면적, 엽중, 근중 모두 Pseudomonas sp. B와 glucosamine oligosaccharide를 혼합한 처리구에서 효과가 컸다. 이상의 결과로 보아 고온기 칼랑코에의 분화 재배시 문제가 되는 생장억제현상은 천연제재와 미생물 제재를 혼합처리 함으로써 크게 개선시킬 수 있었다.

• Plant Resources
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• 제7권3호
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• pp.222-225
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• 2004

In Japan, propagation of cyclamen is mainly from seedlings. However, seeds are expensive and germination is slow and non..uniform. Therefore, to achieve genetically uniform propagation, multiplication must be vegetative. The rooted tuberous stems without shoots as sources of explants were cultured on the media containing BA and sucrose. After 30 days cultivation, tuberous roots were produced from the root ends attached to a tuberous stem and its capability was dependent on the type of media. The highest percentage of tuberous root formation was observed in Culture on the medium of 1/3 MS containing 0.05mgL$^{-1}$ NAA, 0.5mg L$^{-1}$ BA and 5% sucrose. Growth rates of the tuberous roots were greatly influenced by the cutting positions of a root in explants. The highest growth of was observed if small amount of root end was cut at initiation of tissue culture.

• Journal of Microbiology and Biotechnology
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• 제5권4호
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• pp.234-237
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• 1995

Production of new flavanol derivatives with cytotoxic activity, gericudranin A and B, was studied by using hairy root cultures of Cudrania tricuspidata. Schenk and Hildebrandt (SH) medium was chosen for root growth and gericudranin production. After 35 days culture in a half-strength liquid SH medium containing $30g^{glucose}$/l, hairy root growth reached $138g^{FW}$/I and gericudranin A and B were produced at concentrations of 27mg/l and 21 mg/l, respectively. It was also observed that the contents of gericudranin A and B in hairy root were eight and six times higher than those of cudraniae radix, respectively.

• Journal of Ginseng Research
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• 제8권1호
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• pp.57-64
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• 1984

In order to clarify an effect of seedling weight on the growth pattern of ginseng, seedlings ranged from 0.4g plant to 1.8g plant were transplanted, and then the characters of 5- and 6-year-old ginseng were investigated. The characters of root and leaf, such as length and diameter of main root, root weight, leaf area, and leaf dry weight of 5- and 6-year-old ginseng originated from large seedlings were superior as compared with those from small seedlings, and percentage of missing plant was increased with the increase of seedling weight. There were, however, no significant difference in stem length, stem dry weight, number of seeds per plant and number of palmate leaves and leaflets per plant in 5- and 6-year-old ginseng and these characters were not affected by the weight of seedings transplanted. Root field per unit area was higher in seedings of above 0.6g/plant than in small seedlings.

• Journal of Ginseng Research
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• 제15권1호
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• pp.21-30
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• 1991

This study was conducted to know the elects of light intensity of polyethylene net shading on the growth status, photosynthesis and root yield of ginseng plants. Polyethylene net shading of loft transmittance was the best one among light intensities of polyethylene net used in view of photosynthesis and decreasing of early leaf defoliation. According to increase of light intensity under the shading chlorophyll contents of ginseng leaves were decreased. As it was increased over 2 mg/g Photosynthesis and total saponin of leaves showed on the decrease remarkably. The rate of alternaria blight of ginseng plants showed the positive correlation between light intensity and leaking rate. The shading of 10% transmittance in root yield was increased by 40% in 6-year-old ginseng plant as compared with common straw shading, due to decreased missing plant and increased root weight.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.105-109
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• 2006

A transformed hairy root clone of Scutellaria baicalensis was established following infection with Agrobacterium rhizogenes ATCC15834. Three root clones of S baicalensis were selected by growth habit and baicalin content. The most active strain-the SR-03 clone-was examined for its growth and baicalin content under various culture conditions. The root growth and baicalin content were maximized in a Schenk and Hildebrandt medium supplemented with 4 and 6% sucrose, respectively. The accumulation of baicalin in transformed hairy roots was enhanced through exposure to various elicitors. Elicitation was attained by the addition of methyl jasmonate, salicylic acid, and various concentrations of fungal cell wall elicitors to the medium. The accumulation of baicalin in the elicited cultures ranged from 10.5 to 18.3 mg/g dry weight of the roots, which was 1.5- to 3-fold the amount attained in controls.

• Journal of Ginseng Research
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• 제27권4호
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• pp.178-182
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• 2003

Vesicular-arbuscular mycorrhizae (VAM) fungi naturally colonise American ginseng roots and this relationship is highly beneficial to enhance plant productivity. Our goal was to determine the effect of adding two Glomus species (Glomus etuticatum, G. intraradices) on survival, photosynthetic capacity, growth, morphology and root ginsenoside content of one-year-old American ginseng plants grown in a broadleaf forest. While our study revealed that VAM inoculations significantly affected root morphology and Re ginsenoside content, the survival, photosynthetic capacity and root growth of American ginseng plants were not significantly influenced by VAM inoculations. Surface area and volume of rootlets were 16-25% higher for ginseng grown in VAM-inoculated soil compared to those grown in the control plots. Also, Re ginsenoside content was 18 ％ higher in YAM-inoculated roots compared to controls.

• International Journal of Industrial Entomology and Biomaterials
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• 제31권2호
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• pp.107-114
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• 2015

Endophytes provide multifarious benefits such as promotion of plant growth and yield, suppression of phyto-pathogens, phosphate solubilising and fixation nitrogen. A study has been carried out to explore growth promotion and antifungal activities of endophytes of mulberry (Morus spp.). Endophytic bacteria were isolated from mulberry plants and studied their cultural, morphological characters, growth promotion as well as their antifungal activity against Rhizoctonia bataticola and Fusarium oxysporum , two mulberry root rot associated pathogens. Except two isolates, all bacteria were colourless and the colony size of eight isolates was small. The margin of five isolates was irregular and the consistency of three isolates was creamy, six isolates was slimy and one was mucoid. Texture of seven isolates was convex and others were flat. Eight isolates were gram positive and the rest Gram negative, five were cocci and others were bacilli (rod shaped). Four isolates were motile and all were catalase positive and only three isolates were oxidase positive. Spore staining was positive only for two isolates. The growth promotion study showed that there was significant difference in root length and seedling length. The antagonistic effect of the bacterial isolates was tested against R. bataticola showed significant (p <0.05) influence of the bacteria, days after inoculation and their interaction on the inhibition of fungal growth. The isolate En-7 completely inhibited the fungus followed by En-5 (66.67%). The bacterial isolates significantly (p <0.05) inhibited growth of F. oxysporum in PDA. The mean inhibition was higher (70.45%) in case of En-7 followed by En-8 (68.65%) and En-10 (66.44%). The study reveals that some endophytic bacteria associated with mulberry have growth promotion and antifungal activity and could be explored for promotion of mulberry growth and managing root rot disease.