• Korean Journal of Remote Sensing
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• v.33 no.2
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• pp.135-147
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• 2017

We, for the first time, estimated daily and monthly surface nitrogen dioxide ($NO_2$) volume mixing ratio (VMR) using three regression models with $NO_2$ tropospheric vertical column density (OMIT-rop $NO_2$ VCD) data obtained from Ozone Monitoring Instrument (OMI) in Seoul in South Korea at OMI overpass time (13:45 local time). First linear regression model (M1) is a linear regression equation between OMI-Trop $NO_2$ VCD and in situ $NO_2$ VMR, whereas second linear regression model (M2) incorporates boundary layer height (BLH), temperature, and pressure obtained from Atmospheric Infrared Sounder (AIRS) and OMI-Trop $NO_2$ VCD. Last models (M3M & M3D) are a multiple linear regression equations which include OMI-Trop $NO_2$ VCD, BLH and various meteorological data. In this study, we determined three types of regression models for the training period between 2009 and 2011, and the performance of those regression models was evaluated via comparison with the surface $NO_2$ VMR data obtained from in situ measurements (in situ $NO_2$ VMR) in 2012. The monthly mean surface $NO_2$ VMRs estimated by M3M showed good agreements with those of in situ measurements(avg. R = 0.77). In terms of the daily (13:45LT) $NO_2$ estimation, the highest correlations were found between the daily surface $NO_2$ VMRs estimated by M3D and in-situ $NO_2$ VMRs (avg. R = 0.55). The estimated surface $NO_2$ VMRs by three modelstend to be underestimated. We also discussed the performance of these empirical modelsfor surface $NO_2$ VMR estimation with respect to otherstatistical data such asroot mean square error (RMSE), mean bias, mean absolute error (MAE), and percent difference. This present study shows a possibility of estimating surface $NO_2$ VMR using the satellite measurement.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.31 no.4
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• pp.281-286
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• 2009

Introduction: Enamel matrix derivative (EMD) is a protein which is secreted by Hertwig root sheath and plays a major role in the formation of cementum and attachment of peridontium. Several studies have shown that EMD promoted the proliferation and differentiation of preosteoblasts, osteoblasts and periodontal ligament cells in vitro: however, reports showing the inhibition of osteogenic differentiation by EMD also existed. This study was designed to simultaneously evaluate the effect of EMD on the two cell lines (human mesenchymal stem cells: hMSC, human periodontal ligament derived fibroblasts: hPDLCs) by means of quantitative analysis of some bone related matrices (Alkaline phosphatase : ALP, osteopontin ; OPN, osteocalcin ; OC). Materials and Methods: hMSCs and hPDLCs were expanded and cells in the 4${\sim}$6 passages were adopted to use. hMSc and hPDLCs were cultured during 1,2,7, and 14 days with 0, 50 and 100 ${\mu}g/ml$ of EMD, respectively. ALP activity was assessed by SensoLyte ALP kit and expressed as values of the relative optical density. Among the matrix proteins of the bony tissue, OC and OPN were assessed and quantification of these proteins was evaluated by means of human OC immunoassay kit and human OPN assay kit, respectively. Results: ALP activity maintained without EMD at $1,2^{nd}$ day. The activity increased at $7^{th}$ day but decreased at $14^{th}$ day. EMD increased the activity at $14^{th}$ day in the hPDLCs culture. In the hMSCs, rapid decrease was noted in $7^{th}$ and $14^{th}$ days without regard to EMD concentrations. Regarding the OPN synthesis in hPDLCs, marked decrease of OPN was noted after EMD application. Gradual decrease tendency of OPN was shown over time. In hMSCs, marked decrease of OPN was also noted after EMD application. Overall concentration of OPN was relatively consistent over time than that in hPDLCs. Regarding the OC synthesis, in both of hPDLCs and hMSCs, inhibition of OC formation was noted after EMD application in the early stages but EMD exerted minimal effect at the later stages. Conclusion: In this experimental condition, EMD seemed to play an inhibitory role during the differentiation of hMSCs and hPDLCs in the context of OC and OPN formation. In the periodontium, there are many kinds of cells contributing to the regeneration of oral tissue. EMD enhanced ALP activity in hPDLCs rather than in hMSCs and this may imply that EMD has a positive effect on the differentiation of cementoblasts compared with the effect on hMSCs. The result of our research was consistent with recent studies in which the authors showed the inhibitory effect of EMD in terms of the differentiation of mineral colony forming cells in vitro. This in vitro study may not stand for all the charateristics of EMD; thus, further studies involving many other bone matrices and cellular attachment will be necessary.

• Journal of the Korean earth science society
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• v.41 no.5
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• pp.469-477
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• 2020

Salinity is not only an important variable that determines the density of the ocean but also one of the main parameters representing the global water cycle. Ocean salinity observations have been mainly conducted using ships, Argo floats, and buoys. Since the first satellite salinity was launched in 2009, it is also possible to observe sea surface salinity in the global ocean using satellite salinity data. However, the satellite salinity data contain various errors, it is necessary to validate its accuracy before applying it as research data. In this study, the salinity accuracy between the Soil Moisture Active Passive (SMAP) satellite salinity data and the in-situ salinity data provided by the Ieodo ocean research station was evaluated, and the error characteristics were analyzed from April 2015 to August 2020. As a result, a total of 314 match-up points were produced, and the root mean square error (RMSE) and mean bias of salinity were 1.79 and 0.91 psu, respectively. Overall, the satellite salinity was overestimated compare to the in-situ salinity. Satellite salinity is dependent on various marine environmental factors such as season, sea surface temperature (SST), and wind speed. In summer, the difference between the satellite salinity and the in-situ salinity was less than 0.18 psu. This means that the accuracy of satellite salinity increases at high SST rather than at low SST. This accuracy was affected by the sensitivity of the sensor. Likewise, the error was reduced at wind speeds greater than 5 m s^-1. This study suggests that satellite-derived salinity data should be used in coastal areas for limited use by checking if they are suitable for specific research purposes.

• Journal of Periodontal and Implant Science
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• v.26 no.4
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• pp.949-965
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• 1996

The main goal of periodontal therapy is to restore the lost periodontal tissue and establish the attachment appratus. Current acceptable therapeutic techniques are included : removal of diseased soft tissue, demineralization of exposed root surface, using the barrier membrane for preventing the downgrowth of gingival epithelial cell, insertion of graft materials as a scaffolding action, and biological mediators for promoting the cell activity. The latest concept one among them has been studied which based on the knowledge of cellular biology of destructed tissue. Platelet-derived growth factor(PDGF) is one of the polypeptide growth factor which have been reported as a biological mediator to regulate activities of wound healing progress including cell proliferation, migration, and metabolism. The purposes of this study is to evaluate the influences of the PDGF as biological mediator to periodontal ligament and bone marrow cell. Both right and left maxillary first molar were extracted from rat which had treated with 0.4% ${\beta}-Aminopropionitril$ for 5 days, and feeded until designed date to sacrifice under anesthesisa. Periodontal ligament were removed from the extracted socket of the rat, and cultured with Dulbecco's Modified Essential Medium(DMEM) contained with 10% Fetal Bovine Serum, 100U/ml penicillin, $100{\mu}g/ml$ streptomycin, $0.5{\mu}g/ml$ amphotericin-B. Bone marrow cell were culture from bone marrow suspension with which washed out from femur with same medium. The study was performed to evaluate the effect of PDGF to periodontal ligament and bone cell, cell proliferation rate, total protein synthesis, and alkaline phosphatase activity of rat periodontal ligament(PDL) cell and bone stromal(RBS) cell in vitro. The effects of growth factors on both cells were measured at 3, 5th day after cell culture with (control group) or without growth factors(experimental group). The results were as follows: 1. The tendency of cell proliferation under the influence of PDGF showed more rapid proliferation pattern than control at 3 and 5 days after inoculation. 2. The activity of Alkaline phosphatase revealed 14, 80% increased respectively at 3, 5 days culture than control group. Measurements of ALPase levels indicated that PDL cells had significantly higher activity when compared with that of co-culture groups and GF only(P<0.05). And, ALPase activity in 10 days was higher than that of 7 days(P<0.05). 3. The tendency of formation of the mineralized nodule were observed dose-depend pattern of PDL cells. There was statistically significant difference among group l(PDL 100%), 2(PDL 70%:GF 30%), and 3(PDL 50%:GF 50%)(P<0.01). But, there was no difference among group 3, 4(PDL 30%:GF 70%), and 5(GF 100%). 4. Also, the number of nodule was greater in co-culture of PDL 70% and GF 30% than in culture of PDL 70%(P<0.05). From the above results, it is assumed that the PDGF on PDL cells and RMB cell culture. GF stimulates the cell growth, which is not that of PDL cells but GF. And, the activity of ALPase depends on the ratio of PDL cells, and ALPase may relate to the initial phase of nodule formation. Also, it is thought that the calcified nodule formation principally depends on PDL cells, is inhibited by GF, and affected by cell density. In conclusion, platelet-derived growth factor can promote rapid osteogenesis during early stage of periodontal tissue regeneration.

• Korean Journal of Organic Agriculture
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• v.19 no.2
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• pp.215-228
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• 2011

To establish the organic cultivation of pepper using green manure crops, this work studied the growth characteristics and yield of green manure crops, mineral composition of green manure crops, mineral uptake in shoots of green manure crops, chemical composition in soil of green manure crops, and the growth characteristics and yield of pepper in vinyl house. Shoot dry weight of green manure crops was higher level in Sorghum bicolor and Sorghum than in Crotalaria juncea and Glycine max. Also, the roots were spread deeply into soil in Sorghum bicolor and Sorghum. Density of root-knot nematodes in rhizosphere of green manure crops was significantly more decrease in Crotalaria juncea and Sorghum than in Glycine max and Sorghum bicolor. Total nitrogen and CaO content of green manure crops was significantly higher in Crotalaria juncea and Glycine max than in Sorghum bicolor and Sorghum. $K_2O$ content was significantly higher in Sorghum bicolor and Sorghum than in Crotalaria juncea and Glycine max. MgO content was not significant difference at all green manure crops. Cations content ratio of $K_2O$ : CaO : MgO was 3.4 : 1.4 : 1. Total nitrogen uptake in shoots of green manure crops was high level in Glycine max, Sorghum bicolor and Sorghum compared with in Crotalaria juncea. $K_2O$ and MgO uptake was significantly higher in Sorghum bicolor and Sorghum than in Crotalaria juncea and Glycine max. Value of pH in soil of green manure crops was more increase in Crotalaria juncea and Glycine max than in Sorghum bicolor, Sorghum and control, but after cultivation of pepper pH in soil was recovered with initial soil pH before seeding of green manure crops. EC value in control, green manure crops, and pepper cultivation decreased by 44%, 15~18%, and 38~61% level, respectively, compared with initial soil of green manure crops treatment. K content in soil of control, Crotalaria juncea and Glycine max cultivation was increased by 14%, but the K content in soil of Sorghum bicolor and Sorghum decreased by 24~38%. Cation exchange capacity (CEC) in soil of Crotalaria juncea and Sorghum bicolor decreased by 11%, but CEC in soil of Glycine max, Sorghum and control increased by 11%. Harvest fruit yield was higher in Crotalaria juncea, Glycine max, and Sorghum bicolor cultivation than in control and Sorghum.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.5
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• pp.97-104
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• 2017

Greenspace enlargement through proper landscape planting is essential to creating a low carbon society. This study analyzed changes in stem diameter growth rates(DGR), ratios of below ground/above ground biomass(B/A), and carbon sequestration by age of major landscape tree species. Landscape trees for study were 11 species and 112 individuals planted in middle region of Korea. The DGR and B/A were analyzed based on data measured through a direct harvesting method including root digging. The carbon sequestration by tree age was estimated applying the derived regression models. The annual DGR at breast height of trees over 30 years averaged 0.72 cm/yr for deciduous species and 0.83 cm/yr for evergreen species. The B/A of the trees over 30 years averaged 0.23 for evergreen species and 0.40 for deciduous species, about 1.7 times higher than evergreen species. The B/A by age in this study did not correspond to the existing result that it decreased as tree ages became older. Of the study tree species, cumulative carbon sequestration over 25 years was greatest with Zelkova serrata(198.3 kg), followed by Prunus yedoensis(121.7 kg), Pinus koraiensis(117.5 kg), and Pinus densiflora (77.4 kg) in that order. The cumulative carbon sequestration by Z. serrata offset about 5% of carbon emissions per capita from household electricity use for the same period. The growth rates and carbon sequestration for landscape trees were much greater than those for forest trees even for the same species. Based on these results, landscape planting and management strategies were explored to improve carbon sequestration, including tree species selection, planting density, and growth ground improvement. This study breaks new ground in discovering changes in growth and carbon sequestration by age of landscape trees and is expected to be useful in establishing urban greenspaces towards a low carbon society.

• Korean Journal of Agricultural and Forest Meteorology
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• v.13 no.4
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• pp.176-184
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• 2011

Cold air on sloping surfaces flows down to the valley bottom in mountainous terrain at calm and clear nights. Based on the assumption that the cold air flow may be the same as the water flow, current models estimate temperature drop by regarding the cold air accumulation at a given location as the water-like free drainage. At a closed catchment whose outlet is blocked by man-made obstacles such as banks and roads, however, the water-like free drainage assumption is no longer valid because the cold air accumulates from the bottom first. We developed an empirical model to estimate quantitatively the effect of cold pool on nocturnal temperature in a closed catchment. In our model, a closed catchment is treated like a "vessel", and a digital elevation model (DEM) was used to calculate the maximum capacity of the cold pool formed in a closed catchment. We introduce a topographical variable named "shape factor", which is the ratio of the cold air accumulation potential across the whole catchment area to the maximum capacity of the cold pool to describe the relative size of temperature drop at a wider range of catchment shapes. The shape factor is then used to simulate the density profile of cold pool formed in a given catchment based on a hypsometric equation. The cold lake module was incorporated with the existing model (i.e., Chung et al., 2006), generating a new model and predicting distribution of minimum temperature over closed catchments. We applied this model to Akyang valley (i.e., a typical closed catchment of 53 $km^2$ area) in the southern skirt of Mt. Jiri National Park where 12 automated weather stations (AWS) are operational. The performance of the model was evaluated based on the feasibility of delineating the temperature pattern accurately at cold pool forming at night. Overall, the model's ability of simulating the spatial pattern of lower temperature were improved especially at the valley bottom, showing a similar pattern of the estimated temperature with that of thermal images obtained across the valley at dawn (0520 to 0600 local standard time) of 17 May 2011. Error in temperature estimation, calculated with the root mean square error using the 10 low-lying AWSs, was substantially decreased from $1.30^{\circ}C$ with the existing model to $0.71^{\circ}C$ with the new model. These results suggest the feasibility of the new method in predicting the site-specific freeze and frost warning at a closed catchment.

• The Korean Journal of Mycology
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• v.20 no.4
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• pp.337-346
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• 1992

Trichoderma spp. are an effective control agent for damping-off or other plant diseases. The interaction between. T. hamatum and Rhizoctonia solani on the rhizosphere or surface soil were examined to assess the possible roles of antibiosis or competition in the mechanisms of biological control agents as a basic research. In a proportional comparison, total bacteria, fungi, actinomycetes and Trichoderma spp were 65%, 8.8%, 25.9% and 0.28% respectively in their distribution in the soil. Among Trichoderma spp isolated, the 5 species of Trichoderma spp were indentified as T. koninggii, T. pseudokoninggii, T. aureoviridi, T. hamatum and T. viride respectively. In a mycoparasitic test, one isolate of T. hamatum strain Tr-5 showed an enzymatic ability to break fungal hyphae into piecies and infected on the R. solani hyphae showing a parasitism. Spore germination of the all isolates of Trichoderma spp showed a 1.7-7.3% of germination in natural soil conditions, but the percentage was high in sterile soil indicating all the natural soil were fungistatic on conidia of Trichoderma spp. In rhizosphere competent assay in pea plant, the antagonistic T. hamatum, T. viride, T. koninggii, T. pseudokoninggii showed a colonizing upper soil depth in rhizosphere around 1-3 cm in root zone, but the colonizing ability was much reduced along the deeper the soil depth. Propagule density was decreased in deeper the soil layer. Disease development rate treated alone with plant pathogens, Fusarium solani, Rhizoctonia solani, Cylindrocarpon destructans increased, but disease incidence rate reduced in treatment with combinations with antagonistic T. hamatum strain Tr-5.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.2
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• pp.72-78
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• 2009

The demand for rainfall data in gridded digital formats has increased in recent years due to the close linkage between hydrological models and decision support systems using the geographic information system. One of the most widely used tools for digital rainfall mapping is the PRISM (parameter-elevation regressions on independent slopes model) which uses point data (rain gauge stations), a digital elevation model (DEM), and other spatial datasets to generate repeatable estimates of monthly and annual precipitation. In the PRISM, rain gauge stations are assigned with weights that account for other climatically important factors besides elevation, and aspects and the topographic exposure are simulated by dividing the terrain into topographic facets. The size of facet or grid cell resolution is determined by the density of rain gauge stations and a $5{\times}5km$ grid cell is considered as the lowest limit under the situation in Korea. The PRISM algorithms using a 270m DEM for South Korea were implemented in a script language environment (Python) and relevant weights for each 270m grid cell were derived from the monthly data from 432 official rain gauge stations. Weighted monthly precipitation data from at least 5 nearby stations for each grid cell were regressed to the elevation and the selected linear regression equations with the 270m DEM were used to generate a digital precipitation map of South Korea at 270m resolution. Among 1.25 million grid cells, precipitation estimates at 166 cells, where the measurements were made by the Korea Water Corporation rain gauge network, were extracted and the monthly estimation errors were evaluated. An average of 10% reduction in the root mean square error (RMSE) was found for any months with more than 100mm monthly precipitation compared to the RMSE associated with the original 5km PRISM estimates. This modified PRISM may be used for rainfall mapping in rainy season (May to September) at much higher spatial resolution than the original PRISM without losing the data accuracy.

• Horticultural Science & Technology
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• v.28 no.5
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• pp.836-844
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• 2010

This study was carried out to investigate plant characteristics and nutritional components of the genetically modified (GM) Chinese cabbage and its control line grown in the central and northern parts of Korea in order to establish the evaluating protocol and standard assessment. The GM and non-GM Chinese cabbage was planted with normal and concentrated density at two locations in spring and fall of 2008 and 2009. From the statistic analysis on plant characteristics and nutritional components, there were not many significant differences between GM and non-GM Chinese cabbage. Only few differences in the plant characteristics were found between the dense and normal planting. In the dense planting, there was no significant difference between GM and non-GM Chinese cabbages except for three out of 18 plant traits, such as leaf shape, hairiness and midrib length. On the other hand, nine plant traits including leaf length, leaf width, leaf color, leaf shape, fresh weigh of ground part, number of leaf, midrib length, midrib width and root diameter were slightly different between GM and non-GM Chinese cabbage in the normal planting. In case of leaf length, midrib length, midrib width and fresh weigh of ground part, there were significantly differences not only between two lines, but also between two locations. From nutritional component analysis, only five fatty acids were identified in the Chinese cabbage: palmitic acid, oleic acid, stearic acid, linoleic acid and linolenic acid. Except linoleic acid, four fatty acids in one gram of dried sample from GM line were little higher than those from non-GM line. However, there were no significant differences in total contents of fatty acids not only between GM and non-GM Chinese cabbage line, but also between northern and central cultivating areas in the normal and dense planting. According to the composition of inorganic elements identified in the samples from both lines, there were six macro-elements, such as N, P, Ca, K, Mg and Na, and four micro-elements, Cu, Fe, Mn and Zn. Based on the result from PCA analysis, specific clusters were not found between GM Chinese cabbage and the control line, but found between two regions.