• Title/Summary/Keyword: Root canal sealers

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Effects of radiation therapy on the dislocation resistance of root canal sealers applied to dentin and the sealer-dentin interface: a pilot study

  • Pallavi Yaduka;Rubi Kataki;Debosmita Roy;Lima Das;Shachindra Goswami
    • Restorative Dentistry and Endodontics
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    • v.46 no.2
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    • pp.22.1-22.12
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    • 2021
  • Objectives: This study evaluated and compared the effects of radiation therapy on the dislocation resistance of AH Plus and BioRoot RCS applied to dentin and the sealer-dentin interface. Materials and Methods: Thirty single-rooted teeth were randomly assigned to 2 groups (n = 15 each): AH Plus (Dentsply DeTrey) and BioRoot RCS (Septodont). Each group was subdivided into control and experimental groups. The experimental group was subjected to a total radiation dose of 60 Gy. The root canals of all samples were cleaned, shaped, and obturated using the single-cone technique. Dentin slices (1 mm) were sectioned from each root third for the push-out test and scanning electron microscopy (SEM) was done to examine the sealer-dentin interface. The failure mode was determined using stereomicroscopy. Bond strength data were analyzed by the independent t-test, 1-way analysis of variance, and the Tukey post hoc test (α = 0.05). Results: Significantly lower bond strength was observed in irradiated teeth than non-irradiated teeth in the AH Plus group (p < 0.05). The BioRoot RCS group showed no significant reduction in bond strength after irradiation (p > 0.05) and showed a higher post-irradiation bond strength (209.92 ± 172.26 MPa) than the AH Plus group. SEM revealed slightly larger gap-containing regions in irradiated specimens from both groups. Conclusions: The dislocation resistance of BioRoot RCS was not significantly changed by irradiation and was higher than that of AH Plus. BioRoot RCS may be the sealer of choice for root canal treatment in patients undergoing radiation therapy.

In vitro evaluation of a newly produced resin-based endodontic sealer

  • Song, Yoo-Seok;Choi, Yoorina;Lim, Myung-Jin;Yu, Mi-Kyung;Hong, Chan-Ui;Lee, Kwang-Won;Min, Kyung-San
    • Restorative Dentistry and Endodontics
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    • v.41 no.3
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    • pp.189-195
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    • 2016
  • Objectives: A variety of root canal sealers were recently launched to the market. This study evaluated physicochemical properties, biocompatibility, and sealing ability of a newly launched resin-based sealer (Dia-Proseal, Diadent) compared to the existing root canal sealers (AHplus, Dentsply DeTrey and ADseal, Metabiomed). Materials and Methods: The physicochemical properties of the tested sealers including pH, solubility, dimensional change, and radiopacity were evaluated. Biocompatibility was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For microleakage test, single-rooted teeth were instrumented, and obturated with gutta-percha and one of the sealers (n = 10). After immersion in 1% methylene blue solution for 2 weeks, the specimens were split longitudinally. Then, the maximum length of staining was measured. Statistical analysis was performed by one-way analysis of variance followed by Tukey test (p = 0.05). Results: Dia-Proseal showed the highest pH value among the tested sealers (p < 0.05). ADseal showed higher dimensional change compared to AHplus and Dia-Proseal (p < 0.05). The solubility values of AHplus and Dia-Proseal were similar, whereas ADseal had the lowest solubility value (p < 0.05). The flow values of sealer in increasing order were AHplus, DiaProseal, and ADseal (p < 0.05). The radiopacity of AHplus was higher than those of ADseal and Dia-Proseal (p < 0.05). The cell viability of the tested materials was statistically similar throughout the experimental period. There were no significant differences in microleakage values among the tested samples. Conclusions: The present study indicates that Dia-Proseal has acceptable physicochemical properties, biocompatibility, and sealing ability.

Cytotoxicity and Genotoxicity of Newly Developed Calcium Phosphate-based Root Canal Sealers (신개발 인산칼슘계 근관 봉함재의 세포독성 및 유전독성에 관한 연구)

  • Kim, Hee-Jung;Baek, Seung-Ho;Bae, Kwang-Shik
    • Restorative Dentistry and Endodontics
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    • v.31 no.1
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    • pp.36-49
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    • 2006
  • The purpose of this study was to compare the cytotoxicity by MTT test and genotoxicity by Ames test of new calcium phosphate-based root canal sealers (CAPSEAL I, CAPSEAL II) with commercially available resin-based sealers (AH 26, AH Plus) , zinc oxide eugenol-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT), calcium hydroxide-based sealer (Sealapex), and tricalcium phosphate based sealers (Sankin Apatite Root Canal Sealer I, II, III). According to this study, the results were as follows : 1. The extracts of freshly mixed group showed higher toxicity than those of 24 h set group in MTT assay (p<0.001). 2. CAPSEAL I and CAPSEAL II were less cytotoxic than AH 26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT Sealapex and SARCS II in freshly mixed group (p<0.01). 3. AH 26 in freshly mixed group showed mutagenicity to TA98 and TA100 with and without S9 mix and AH Plus extracts also were mutagenic to TA100 with and without S9 mix. 4. Tubliseal EWT, Pulp Canal Sealer EWT and Sealapex in freshly mixed group were mutagenic to TA100 with S9 mix. 5. Among those of 24 h set groups the extracts of SARCS II were mutagenic to TA98 with and without S9 mix and AH 26 showed mutagenic effects to TA98 with S9 mix. 6. No mutagenic effect of CAPSEAL I and CAPSEAL II was detected. 7. There is no statistically significant difference between CAPSEAL I and CAPSEAL II at MTT assay and Ames test in both freshly mixed group and 24 h set group.

Micro-computed tomographic evaluation of a new system for root canal filling using calcium silicate-based root canal sealers

  • Tanomaru-Filho, Mario;Torres, Fernanda Ferrari Esteves;Pinto, Jader Camilo;Santos-Junior, Airton Oliveira;Tavares, Karina Ines Medina Carita;Guerreiro-Tanomaru, Juliane Maria
    • Restorative Dentistry and Endodontics
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    • v.45 no.3
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    • pp.34.1-34.7
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    • 2020
  • Objectives: This study evaluated by using micro-computed tomography (micro-CT) the filling ability and sealer apical extrusion promoted by a new Sealer Injection System (SIS; Angelus) with side openings needle, in comparison with the conventional injection system, associated with a new ready-to-use calcium silicate-based sealer (Bio-C Sealer). Materials and Methods: Acrylic resin models containing a main curved artificial canal and 3 simulated lateral canals in apical, middle and cervical thirds were used. The main root canals were prepared using a rotary system up to size 35.05. The canals were filled with Bio-C sealer by using a single cone technique and the conventional delivery system or SIS. Samples were scanned in micro-CT. The percentage of voids throughout the entire extension of the main root canal and in each third of the lateral canals, besides the apical extrusion of the sealer was calculated. Data were submitted to t-test (p < 0.05). Results: There was no difference between both systems in the main root canals filling. Although the volume percentage of voids was similar in the apical and middle thirds of lateral canals, SIS had the greatest filling ability of the cervical third lateral canal. Moreover, the conventional system showed the highest apical extrusion of the sealer. Conclusions: The conventional and SIS obturation systems had an appropriate filling ability of the main root canal. SIS had the best filling of the cervical third of the lateral canals, besides lower sealer apical extrusion, suggesting its clinical indication.

A COMPARATIVE STUDY ON RADIOPACITY OF ROOT CANAL SEALERS (근관 전색재의 방사선 불투과성에 관한 비교연구)

  • Kim, Tae-Min;Kim, Seo-Kyoung;Hwang, In-Nam;Hwang, Yun-Chan;Kang, Byung-Cheol;Yoon, Suk-Ja;Lee, Jae-Seo;Oh, Won-Mann
    • Restorative Dentistry and Endodontics
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    • v.34 no.1
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    • pp.61-68
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    • 2009
  • This study was performed to assess the radiopacity of a variety of root canal sealers according to the specification concerning root canal sealers. Ten materials including Tubli-$Seal^{TM}$. Kerr Pulp Canal $Sealer^{TM}$, AH $26^{(R)}$, AH $plus^{(R)}$, AH plus $jet^{TM}$, Ad sea $1^{TM}$. $Sealer^{TM}$, $NOGENOL^{TM}$, ZOB $seal^{TM}$, $Epiphany^{TM}$ and dentin were evaluated in this study. In the first part, densitometric reading of an each step of aluminum step wedge on occlusal film was performed at different voltage and exposure time. In the second part, ten specimens were radiographed simultaneously with an aluminum step wedges on the occlusal films under decided condition. The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (mm Al). The following results were obtained. 1. Among the various conditions, the appropriate voltage and exposure time that meet the requirement density was 60 kVp at 0.2 s 2. All of the materials had greater radiopacity than 3 mm Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards. 3. The radiopacity of materials increased as thickness of materials increased. 4. The mm Al value of each specimen at 1mm in thickness has a significant difference in the statistics. It suggests that root canal sealers have a sufficient radiopacity that meet the requirement.

HISTOLOGIC STUDY ON THE PERIODONTAL TISSUE REACTIONS OF THE ROOT PERFORATIONS (치근관(齒根管) 천공(穿孔)에 의(依)한 치주조직(齒周組織) 변화(變化)에 관(關)한 조직학적(組織學的) 연구(硏究))

  • Park, Sang-Jin
    • Restorative Dentistry and Endodontics
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    • v.6 no.1
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    • pp.7-16
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    • 1980
  • The author observed the periodontal tissue reactions to the root canal sealers after root perforations were made intentionally in dogs. The perforations were made on 74 teeth from 7 dogs. The experiments were performed in two different modes of procedure: In Group I, the perforations were made through the root canal to the alveolar bone. In Group II, the perforations were made from site of alveolar bone to the root canals. The perforated canals in Group I were filled with gutta-percha and root canal cements; Calxyl (Calcium Hydroxide in Ringer's solution), Zinc Oxide -Eugenol cement (Z.O.E.), Kerr sealer (Rickert's paste) and AH 26 (Epoxy Resin preparations). The perforated canals in Group II were sealed with Calxyl, Z.O.E, Kerr sealer and AH26. Histologic examinations of periodontal tissue reactions were observed at various time intervals. The results were as follows; l. Cementum deposition on the perforated root surface in Group II cases showed slightly earlier than that of Group I. Healing tendency of injured alveolar bone in Group II was greater than that of Group I. 2. According to the time increase after experiment, the cementum deposition on the site of perforated dentin in Group II with intact pulp was notably thickened. Secondary dentin deposition on the root canal surface where the dentinal tubles were cut was also found in similar pattern. 3. In the cases of perforated canals sealed with Calxyl both in Group I and Group II, It revealed the earliest cementum-deposition among 4 different root canal cements. In the cases of perforated canals sealed with Kerr sealer and AH26, the cementum-deposition on the root surface was not found. 4. Proliferation of epithelium around the perforated area was first seen at 5-week cases in Group I, and at 3-week cases in Group II. 5. In all cases, dentin resorption on the site of perforated root surface was always occured.

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AUTORADIOGRAPHIC STUDY ON SEALING ABILITY OF VARIOUS ROOT CANAL SEALERS (수종(數種) 근관충전재(根管充填材)의 근관폐쇄(根管閉鎖) 효과(效果)에 관(關)한 자기(自記) 방사법적(放射法的) 연구(硏究))

  • Choi, Woong-Dai;Park, Sang-Jin;Min, Byung-Soon;Choi, Ho-Young
    • Restorative Dentistry and Endodontics
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    • v.14 no.1
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    • pp.161-171
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    • 1989
  • The purpose of this study was to evaluate and compare the sealing ability of ZOE, FR and Sealapex, as a root canal sealer. Ninety six extracted anterior or premolar human teeth with single root were instrumented by using conventional method. After instrumentation, the teeth were divided into three groups and thirty two teeth in each group were filled as follows: ZOE group: ZOE cement as a root canal sealer in combination with guttapercha cone FR group: FR root canal sealer in combination with guttapercha cone Sealapex group: Sealapex root canal sealer in combination with guttapercha cone $^{45}Ca$ in the form of calcium chloride, was employed as the tracer in this study and produce the auto-radiograph. The depth of isotope penetration into the root canal was evaluated by method by Yates and Hembree at the intervals of 1 day, 7 days, 14 days and 30 days. The following conclusions were derived from the results obtained; 1. After 1 day, ZOE group exhibited the greatest penetration degree of radioisotope (p < 0.05). 2. After 14 days, there was some difference of the radioisotope penetration between each group, but its difference was not significant statistically. 3. After 30 days Sealapex group exhibited the least penetration degree of radioisotope (p < 0.025). 4. In ZOE and Sealapex groups, there was no change of the degree of radioisotope penetration with time.

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EXPERIMENTAL STUDY on SEALING QUALITY OF KERR SEALER AS ROOT CANAL SEALERS (Kerr sealer를 근관충전재(根管充塡材)로 사용시(使用時) 근관폐쇄성(根管閉鏁性)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Yoon, Soo-Han
    • Restorative Dentistry and Endodontics
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    • v.6 no.1
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    • pp.77-81
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    • 1980
  • The author has studied comparatively the sealing quality of conventional Z.O.E. cement, Kerr sealer and Nogenol sealer by means of penetration of 2% methylene blue solution through the root apex of human teeth in 72 cases as time elapsed. The results were as follows; 1. All the specimens, whose dye immersion time is two days, showed dye penetration of low degree unrelated to the kinds of root canal sealer. 2. Kerr sealer group, like conventional Z.O.E. group, showed dye penetration of high degree related to increased time of immersion in the dye. 3. In Nogenol group, there was slight increase in penetration related to increased time of immersion in the dye. 4. Kerr sealer group showed comparatively lower grade of dye penetration than conventional Z.O.E. and higher than Nogenel sealer.

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THE CYTOTOXIC EFFECTS OF ROOT CANAL SEALERS ON FIBROBLASTS IN HUMAN PERIODONTAL LIGAMENT (근관 충전용 시멘트의 치주인대 섬유아세포 독성에 관한 연구)

  • Lee, Gwang-Hee;Choi, Ho-Young;Min, Byung-Soon;Park, Sang-Jin;Choi, Gi-Woon
    • Restorative Dentistry and Endodontics
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    • v.18 no.1
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    • pp.156-172
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    • 1993
  • The purpose of this study was to evaluate for the cytotoxicity of root canal sealers - Tubliseal, N2, AH26, and Sealapex - on fibroblasts cultivated from human periodontal ligament. Succinate dehydrogenase activity test and $^{51}Cr$ release test were performed to evaluate the shortterm cytotoxicity. According to l,3 and 6 days vital cell count and $^{14}C$-leucine incorporation rate to fibroblasts were evaluated. The results of this study were as follows: 1. In the test of SDH activity by millipore filter method, Sealapex showed mild cytotoxicity but Tubliseal, N2 and AH26 showed severe cytotoxicity. 2. In $^{51}Cr$ release test, Tubliseal was the most cytotoxic sealer tested, and rank ordered the relative cytotoxicity of the other sealers tested as follows: AH26, N2, Sealapex. 3. In the test of viable cell count, cytotoxicity in Tubliseal was continued because vital cell number reduced with time. Because vital cell was not showed in N2 and AH26 at 1 day, it was recognized that N2 and AH26 exhibited severe cytotoxicity. In Sealapex, vital cell number increased remarkably with time, so it showed that cytotoxicity decreased with time. 4. In $^{14}C$-leucine incorporation rate test, protein sythesis was not produced in Tubliseal, N2 and AH26 after 3 days, it showed that cytotoxicity in Tubliseal, N2 and AH26 was severe. Although protein synthesis in Sealapex decreased with time, it continued after 6 days. Therefore Sealapex has been exhibited mild cytotoxicity.

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AN EXPERIMENTAL STUDY OF THE EFFECTS ON THE BONE TISSUE BY IMPLANT OF ROOT CANAL FILLING MATERIALS (근관충전재(根管充塡材)의 매식(埋植)이 골조직(骨組織)에 미치는 영향(影響)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Chung, Jae-Kyoo;Min, Byung-Soon;Chi, Ho-Young;Park, Sang-Jin
    • Restorative Dentistry and Endodontics
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    • v.9 no.1
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    • pp.25-35
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    • 1983
  • The purpose of this study was the observe the toxic effects of root canal sealers in 108 white rats. Experimental animals were divided into control and experimental groups. Theree representative types of materials, such as AH26, Z.O.E. and F.R. were used in this study. Cavities were prepared on the left mandibular area of 108 white rats. Three different sealers were placed in as experiment and bone cavities were left without filling as control. The experimental animals were sacrificed by cervical dislocation at the intervals of 1, 3, 7, 14, 28 and 49 days after filling. Each specimen was fixed with 10% neutral formalin solution, decalcified with 5% nitric acid, embedded in paraffin and sectioned 5-7${\mu}$. in thickness. The paraffin sections stained with Hematoxylin - Eosin were observed through the ordinary light microscope. The results were as follows; 1. Slight toxic effect to surrounding tissue were found in every experimental specimen. 2. AH26 showed the highest inflammatory response, and F.R. showed the lowest inflammatory response which subsided and replaced by fibrosis at 4 weeks after filling. 3. The cavity filled materials, such as implanted root canal sealers, blood clots and necrotic tissue, showed a tendency to be absorbed gradually proportioned to the experimental periods. A small amount of cavity filled materials were observed in the bone cavities after 4 weeks. 4. Fibroblastic proliferation began to produce fibrous capsule around the bone cavity in 2 weeks after filling. Fibrosis was prominent at 4 weeks after filling. 5. Osteoblastic activity of surrounding bone was observed at first in 2 weeks after filling and prominent in 4 weeks after filling. Osteoblastic activity showed an increasing effect as the time prolonged. 6. Surrounding tissue of the bone cavities showed the features of tissue destruction and had very severe inflammatory response at an initial stage. Above-mentioned appeared to be recovered gradually proportioned to the experimental periods.

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