• 원예과학기술지
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• 제35권2호
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• pp.178-187
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• 2017

인공 광원에 따른 rocketsalad(Eruca sativa L.) 내 GSL 함량을 조사하였다. 실험에 사용한 광원의 종류는 자연광(Control-1 또는 2), Red LED, Blue LED, Mix(R+B) LED(Red LED+Blue LED), White LED, Fluorescent Lamp(FL), Fluorescent Lamp+UV-C(FL+UV-C). 실험은 식물 생장기 대수 제한 때문에 [실험 I;Control-1, Red LED, Blue LED, Mix(R+B) LED]과 [실험 II;Control-2, White LED, FL, FL+UV-C]로 구분하여 수행하였다. 그 결과, Red LED와 FL에서 rocket salad의 잎의 길이의 증가율이 가장 높았다. 그러므로 Red LED와 FL이 rocketsalad의 성장과 관계가 있음을 확인했다. Rocketsalad로부터 총 7종류의 GSLs(glucoraphanin, diglucothiobeinin, glucoerucin, glucobrassicin, dimeric 4-mercaptobutyl GSL, 4-methoxy glucobrassicin, gluconasturtiin)를 분리 및 동정하였다. [실험 I]에서 총 GSL 함량은 Red LED(4.30)에서 가장 높고 Blue LED($0.17{\mu}mol{\cdot}g^{-1}\;DW$)에서 가장 낮았다. [실험 II]에서 총 GSL 함량은 FL(13.45)에서 가장 높고 FL+UV-C($0.39{\mu}mol{\cdot}g^{-1}\;DW$)에서 가장 낮았다. 특히 Rocket salad의 강한 향과 매운 맛을 돋아주는 dimeric 4-mercaptobutyl 함량은 [실험 II]에서 Control-2에 비해 FL과 White LED가 각각 14.9, 3.2배 증가했다. 따라서 Red LED, White LED, FL은 rocket salad의 GSL 축적에 영향을 주었다고 판단된다.

• 농업과학연구
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• 제38권2호
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• pp.285-294
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• 2011

Glucosinolate (GSL) contents were investigated (i) at 1~7 days after sowing (DAS) in seed sprouts and (ii) at 3-7 weeks after sowing for the time-course. Moreover, (iii) They were compared with five different cultivars of rocket salad (Eruca sativa). Seventeen GSLs were separated by HPLC analysis, and 10 GSLs among them were identified as glucoraphanin, sinigrin, glucoalyssin, diglucothiobeinin, glucobrassicanapin, glucoerucin, glucobrassicin, dimeric, 4-mercaptobutyl GSL, 4-methoxy glucobrassicin, gluconasturttin by using LC-APCI-MS analysis, but 7 compounds were not identified. (i) The total GSL content in seed sprouts initially increased up to 3 DAS and then decreased according to their seedling growth. In particular, glucoraphanin known as a strong anti-cancer reagent was found the highest level (5.05 ${\mu}mol/g$ dry wt.) at 3 DAS. The most abundant GSL was glucoerucin ranged from 26.0~49.6 ${\mu}mol/g$ dry wt. (ii) In the time-course, the total GSL contents increased dramatically from 3-week (5.91 ${\mu}mol/g$ dry wt.) to 7-week after sowing (32.2 ${\mu}mol/g$ dry wt.). The major GSLs were glucoraphanin, glucoerucin and 4-methoxy glucobrassicin. (iii) By comparing GSL contents with five different cultivars, the total GSL contents increased from 4-week to 6-week after sowing, regardless of cultivar. In 4-week-old, the order with the total GSL content was "Rucola" > "Rocket Herbs" ${\geqq}$ "Odyssey" > "Takii" > "Herb", but in 6-week-old it is changed as "Takii" > "Herb" > "Odyssey" > "Rucola" > "Rocket Herbs" even there was almost no significantly difference between them.