• Title/Summary/Keyword: Ripening Stages

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Purification of Phenylalanine Ammonia-lyase from Strawberry Fruits during Ripening (연화 딸기의 Phenylalanine Ammonia-lyase에 관한 연구)

  • 이광희;윤경영
    • The Korean Journal of Food And Nutrition
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    • v.9 no.4
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    • pp.430-433
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    • 1996
  • The activity of PAL, an initial and key regulatory enzyme in biosynthesis of phenolics, peaked during ripening stage. The enzyme activities were 5.60 and 4.25 units/100g fr. wt. in ripening and overripening stages. The increase in PAL activity in ripening strawberry fruits is due to de novo synthesis of the enzyme. PAL extracts from ripe strawberry fruits have a native molecular weight of about 260, 000 Da.

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Physicochemical quality, antioxidant compounds, and activity of 'Beta Tiny' and 'TY Nonari' cherry tomatoes during storage

  • Joung, Minji;Shin, Youngjae
    • Korean Journal of Food Science and Technology
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    • v.53 no.1
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    • pp.63-71
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    • 2021
  • In this study, a comparative analysis was carried out between the 'Beta Tiny' and 'TY Nonari' cherry tomato cultivars harvested at the pink and red stages. Samples of the red stage were stored at room temperature for 9 days, during which physicochemical qualities, antioxidant compounds, and activities were measured. As cherry tomato ripening and storage progressed, firmness was reduced, whereas the lycopene content increased. Total phenolic content and antioxidant activity showed no significant changes as ripening and storage progressed; however, total flavonoid content of 'Beta Tiny' showed a significant increase (p<0.05). The main polyphenols in the two cultivars were identified as chlorogenic acid, rutin, and (-)-epigallocatechin gallate, among which chlorogenic acid showed a significant decrease (p<0.05) as ripening and storage progressed. A strong correlation was found between total phenolic and flavonoid content (R=0.744), and ABTS radical scavenging activity (R=0.975). Additionally, a negative correlation was shown by lycopene and chlorogenic acid (R= -0.934).

Chilling Responses of Chilli Pepper Fruits at Different Ripening Stages to Low Temperature during Storage

  • Lim, Chae-Shin;An, Chul-Geon;Huh, Moo-Ryong
    • Journal of agriculture & life science
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    • v.44 no.1
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    • pp.1-8
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    • 2010
  • This study was conducted to investigate effect of low temperature and ripeness stages on fruit quality of chilli pepper (cv. Nockgwang) during storage at 5, 7, and $10^{\circ}C$. Fruits at three ripeness stages were selected based on uniform fruit size and three maturities on peel color: S1 (fully developed fruit just before the onset of ripening), S2 (fruits in the onset of color change), and S3 (fruits in completely red in color). Lower temperature attributed to increase fruit weight loss (WL) and WL was higher in S2 than the other ripeness stages. The highest respiration rate and ethylene production was found at S2 fruit while those of fruit in green and red showed similar and remained lower level during storage. Electrolyte leakage (EL) was higher as storage temperature decreased. After 28 d storage, EL rates of fruits at $5^{\circ}C$ were 43, 36, and 17% in S1, S2, and S3 while those at $10^{\circ}C$ were 17, 19, and 14%, respectively. These results show that chilli pepper fruits are tended to lose more water at lower temperature partially associated with increased EL. Thus, threshold storage temperature must be considered for avoiding chilling during storage and for extending the storage life of fresh pepper fruits.

The Chilling Injury Development and Quality Characteristics of 'Ooishiwase' Plums (Prunus salicina L.) according to Ripening Stages and Cold Storage Temperature (자두 '대석조생'의 숙기 및 저온저장 온도에 따른 저온장해과 발생 및 품질특성)

  • Cho, Mi-Ae;Hong, Yoon-Pyo;Choi, Sun-Young;Jung, Dae-Sung;Lim, Byung-Sun;Park, Soo-Jeong;Lee, Seung-Koo
    • Food Science and Preservation
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    • v.18 no.5
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    • pp.651-660
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    • 2011
  • This study was conducted to evaluate the quality characteristics of and chilling injury development in 'Ooishiwase' plum fruits after harvest, according to the ripening stage and storage temperature. The fruits were harvested at three ripening stages (60, 80, and >90% skin color) and were then stored at 1, 4, 5, 6, and $20^{\circ}C$ cold-storage rooms for up to 48 days. The fruit quality parameters, respiration patterns, and chilling injury development were monitored during the storage periods and the three days of subsequent ripening at $20^{\circ}C$. The fruits harvested at the 60%-skin-color stage maintained the flesh firmness, color, weight loss, and TA, and their respiration rates and ethylene production were decreased compared with the 80%-or >90%-skin-color fruits, at a lower storage temperature. The major symptoms of chilling injuries in the Ooishiwase plums were gel breakdown, flesh browning, and flesh translucency. These symptoms appeared at all the low-storage-temperature and ripening treatment stages. When the fruits, however, were harvested at a more immature stage and were stored at a lower storage temperature, the chilling injury development decreased. These results show that the development of chilling injury in Ooishiwase plums is related to the climacteric behavior during cold storage.

Ripening Related Changes in Hot Pepper Fruit Cell Walls Structural Alterations of Cell Wall Polysaccharides and Separation of Galactosidase Isozymes (고추의 성숙에 따른 세포벽 다당류의 변화와 ${\beta}-Galactosidase$ Isozymes의 분리)

  • Kim, Soon-Dong;Kang, Meung-Su;Kim, Kwang-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.2
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    • pp.157-163
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    • 1985
  • Various cell wall polysaccharides and related enzyme activities in hot pepper fruit were determined at different stages of maturity. The uronic acid content of cell walls decreased between immature green and turning stage fruit and then increased by red ripe stage. In contrast, cellulose content of cell walls changed only a little during ripening. Total neutal sugar content of cell wall material decreased 50% and galactose content of the walls decreased about 80% by the turning stage. Polygalacturonase and ${\beta}-galactosidase$ activities, as well as total hemicellulose from isolated cell walls of ripening hot pepper fruit were studied using gel filtration chromatography. Polygalacturonase activity was not detectable but 5 isozymes of ${\beta}-galactosidase$ were resolved. The activities of the enzymes were relatively high and gel filtration showed that they differed in molecular weight. Hemicellulose content decreased during ripening and softening. The molecular weight profiles shifted from high molecular weight to low molecular weight polymers during ripening. The changes in cell walls that may be associated with fruit softening involve the alteration of hemicellulose prior to the degradation of wall-bound uronic acid. It is suggested that the decrease in cell wall galactose involved changes in turnover of new cell wall components.

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Characteristics of Rubus coreanus Miq. Fruits at Different Ripening Stages (숙성에 따른 토종 복분자 딸기의 특성)

  • Kim, Ji-Myoung;Shin, Mal-Shick
    • Korean Journal of Food Science and Technology
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    • v.43 no.3
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    • pp.341-347
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    • 2011
  • To develop a healthy functional food containing Rubus coreanus fruit (RCF) powder with different degrees of ripening, the composition, physicochemical properties, and antioxidant activity of native RCF during ripening were investigated and compared with Rubus occidentalis fruit (ROF). Ripened RCF was bright red in color, whereas ripened ROF was dull and dark red in color. The seeds of RCF were softer and smaller than those of ROF. Crude protein, crude lipid, and total sugar content of RCF powder increased with increased ripening time, whereas ash and total dietary fiber contents decreased with increased ripening time. Color differences increased with Increased ripening. Eighteen types of amino acids were analyzed from RCF, and glutamic acid had the highest content. RCF powder had the highest level of potassium and calcium and did not contain any hazardous metals. Mineral and organic acid contents decreased according to ripening.

Plant Growth Regulation by Uniconazole-P Application and its Effects on Seed Production in Pasture Plants (식물생장조절제 Uniconazole-P에 의한 생육억제와 목초의 종자 생산)

  • Lee, Ju Sam
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.18 no.4
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    • pp.291-302
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    • 1998
  • The purpose of this study is to clarify the effect of plant growth regulator "Uniconazole-P" on the control of growth and seed producrtion of pasture plants under grown in sward conditions. Four species examined were orchard grass, timothy, red clover and alfalfa. Uniconazole-P concentrations were control(0), 20ppm and 40ppm, and foliar sprayed on canopy structures at the floral differentiation stages of grasses and at the begining of flowering stages of legumes, respectively. 1. Yield components and seed yield components of grasses and legumes were responded differently between Uniconazole-P concentrations, species and the stages of growth. 2. At early heading stages, the plant length and culm length of grasses were reduced by Uniconazole-P treatments. On the contrary, the dry weight of ears per area and chlorophyll concentrations were increased by Uniconazole-P treatments. 3. At seed ripening stages, the number of ears, dry weight of a tiller, dry weight of a ear, dry weight of ears per area and dry weight of seeds in orchardgrass, and the number of ears, dry weight of a ear, dry weight of ears per area, dry weight of seeds and harvest index in timothy were increased by Uniconazole-P treatments. 4. At early flowering stages, the plant length and total length of internodes were reduced by Uniconazole-P treatments. On the contrary, total length of branches and chlorophyll concentrations of red clover and alfalfa were increased by Uniconazole-P treatments. Particularly, the number of inflorescences and dry weight of inflorescences of red clover was increased greatly by Uniconazole-P treatments. 5. At seed ripening stages, the plant length of both of legumes were reduced by Uniconazole-P treatments. On the contrary, the dry weight of a inflorescence, dry weight of inflorescences per area, dry weight of seeds and harvest index of alfalfa was increased by Uniconazole-P treatments. 6. Seed production of grasses by Uniconazole-P treatments can be explained as following processes at each stage of growth. 1) reduced in plant length and culm lengths at early heading stages, 2) increased in number of ears and dry weight of a ear at both of stages, and 3) increased in dry weight of ears per area, dry weight of seeds and harvest index at seed ripening stages. 7. Seed production of legumes by Uniconazole-P treatments can be explained as following processes at each stage of growth. 1) reduced in plant length and total length of internodes and increased in number of branches and total length of branches at early flowering stages, 2) increased in number of inflorescences and dry weight of inflorescences at both of stages, and 3) increased in dry weight of seeds and harvest index at seed ripening stages.

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cDNA cloning and expression pattern of Cinnamate-4-Hydroxylase in the Korean black raspberry

  • Baek, Myung-Hwa;Chung, Byung-Yeoup;Kim, Jin-Hong;Kim, Jae-Sung;Lee, Seung-Sik;An, Byung-Chull;Lee, In-Jung;Kim, Tae-Hoon
    • BMB Reports
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    • v.41 no.7
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    • pp.529-536
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    • 2008
  • Cinnamate-4-hydroxylase (C4H) is a key enzyme in the phenylpropanoid pathway, which is responsible for synthesizing a variety of secondary metabolites that participate in development and adaptation. In this study, we isolated a full-length cDNA of the C4H gene from the Korean black raspberry (Rubus sp.) and found that this gene existed as a single gene. By comparing the deduced amino acid sequence of Rubus sp. C4H with other sequences reported previously we determined that this sequence was highly conserved among widely divergent plant species. In addition, quantitative real time PCR studies indicated that the C4H gene had a differential expression pattern during fruit development, where gene expression was first detected in green fruit and was then remarkably reduced in yellow fruit, followed by an increase in red and black fruit. To investigate the two peaks in expression observed during fruit development and ripening, we measured the flavonoid content. The content of the major flavanol of Korean black raspberry fruits was determined to be highest at the beginning of fruit development, followed by a gradually decrease according to the developmental stages. In contrast, the content of anthocyanins during the progress of ripening was dramatically increased. Our results suggest that the C4H gene in Korean black raspberry plays a role during color development at the late stages of fruit ripening, whereas the expression of C4H gene during the early stages may be related to the accumulation of flavanols.

Seed Germination Rate and Growth Characteristics according to Ripening Stages in Angelica acutiloba Kitagawa (일당귀의 등숙에 따른 종자 발아 및 생육 특성)

  • Lee, Eun Song;An, Tae Jin;Kim, Yong Il;Park, Woo Tae;Lee, Jeong Hoon;Kim, Young Guk;Chang, Jae Ki;Oh, Myung Min
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.3
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    • pp.167-172
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    • 2019
  • Background: Angelica acutiloba Kitagawa belongs to the flowering plant family Apiaceae. Its young leaves are consumed as a vegetable, and its roots have medicinal properties. This study was conducted to analyze the seed germination characteristics of A. acutiloba according to its ripening stages. Methods and Results: The seeds were harvested from a research farm managed by the Department of Herbal Crop Research, Rural Development Administration in 2018 and were divided into six groups according to their specific gravities. In particular, we studied the effect of harvesting periods and umbel orders on seed characteristics. The results showed that the rates of germination, emergence, and early growth improved as the specific gravity of the seeds increased. In addition, the germination rates of the seeds harvested in mid July and early August were significantly higher than those harvested in the other seasons, and the seeds obtained from the first floret had the greatest mass and weight. Conclusions: In this study, we demonstrated that it is possible to improve the germination rate by appropriate selection of seeds and harvesting period both of which are closely related to seed maturity.