• IMMUNE NETWORK
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• v.2 no.3
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• pp.137-141
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• 2002

Among the members of the inhibitor of apoptosis (IAP) protein family, only Livin and survivin have been reported to have variant forms. We have found a variant form of c-IAP2 through the interaction with the X protein of HBV using the yeast two-hybrid system. In contrast to the wild-type c-IAP2, the variant form has two stretches of sequence in the RING domain that are repeated in the C-terminus that would disrupt the RING domain. We demonstrate that the variant form has an inhibitory effect on TNF-mediated $NF-{\kappa}B$ activation unlike the wild-type c-IAP2, which increases TNFmediated $NF-{\kappa}B$ activation. These results suggest that this variant form has different activities from the wild-type and the RING domain may be involved in the regulation of TNF-induced $NF-{\kappa}B$ activation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.12A
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• pp.1177-1181
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• 2006

In this paper, we proposed the new wavelength tunable filter for wide tunability of fiber ring laser. The proposed filter consists of the cascaded connection of Fiber Reflection Mach-Zehnder Interferometer and Sagnac filter or of hybrid filter and fiber Reflection Mach-Zehnder Interferometer. The simulation shows that the continuous tunable range of 50nm can be obtained and that more stable single mode operation of ring laser can be expected due to narrow FWHM. of filter spectrum.

• Journal of Institute of Control, Robotics and Systems
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• v.19 no.11
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• pp.960-967
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• 2013

Shape recognition of solder ball bumps in a BGA (Ball Grid Array) is an important issue in flip chip bonding technology. In particular, the semiconductor industry has required faster and more accurate inspection of micron-size solder bumps in flip chip bonding as the density of balls has increased dramatically. The difficulty of this issue comes from specular reflection on the metal ball. Shape recognition of a metal ball is a very realproblem for computer vision systems. Specular reflection of the metal ball appears, disappears, or changes its image abruptly due to tiny movementson behalf of the viewer. This paper presents a practical shape recognition method for three dimensional (3-D) inspection of a BGA using a 5-step ring illumination device. When the ring light illuminates the balls, distinctive specularity images of the balls, which are referred to as "iso-slope contours" in this paper, are shown. By using a mathematical reflectance model, we can drive the 3-D shape information of the ball in aquantitative manner. The experimental results show the usefulness of the method for industrial application in terms of time and accuracy.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.226-231
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• 2004

Press fitting method for joining of a hybrid tube and steel ring with small teeth for automotive aluminum/composite hybrid propeller shaft was devised to improve reliability and to reduce manufacturing cost, compared to other joining methods such as an adhesively bonded joint, bolted joint or welded joint. To obtain high strength of the press fit joint, an optimal design method for the teeth was devised with respect to number and shape of the steel teeth. Torsional static, fatigue tests and finite element analysis of the press fit joint were performed with respect to experimental variables. The developed optimal design method predicted well the static torque capability and failure mode of the press fit joint. Also, it provided design guide line of press fit joint for improving torsional static and fatigue characteristics.

• International Journal of Precision Engineering and Manufacturing
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• v.2 no.2
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• pp.26-30
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• 2001

This paper deals with the feasibility assessment of hybrid linear motor that operates based on self-moving cell concept. The moving cell is composed of Magnetostrictive actuator and a ring structure, and a cell train is constructed by connecting two cells in series. Since this motor uses strong push force of Terfenol-D actuators and friction of the cells, it can essentially produce long stroke and large force. The overall performance of the motor was measured in terms of speed and force.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.11a
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• pp.253-256
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• 2006

The HPFRCCs show the multiple crack and damage tolerance capacity due to the interfacial bonding of the fibers to the cement matrix. For practical application, it is needed to investigate the fractural behavior and of HPFRCCs and understand the micro-mechanism of cement matrix with reinforcing fiber. The objectives of this paper are to examine the compressive behavior, fracture and damage process of HPFRCC by acoustic emission technique. Total four series were tested, and the main variables were the hybrid type, polyethylene (PE) and steel cord (SC), and fiber volume fraction. The damage progress by compressive behavior of the HPFRCCs is characteristic for the hybrid fiber type and volume fraction. And from acoustic emission (AE) parameter value, it is found that the second and third compressive load cycles resulted in successive decrease of the ring-down count rate as compared with the first compressive load cycle.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.389-392
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• 2004

It is clinically well known that pre-tension of wires increases the fracture stability in ring or hybrid external fixation. In some cases, additional half pin should be necessary to increase the stability when soft tissue impalement occurs during fixation. In this paper, the fracture stability of a hybrid external fixator system with different pre-tension effects and additional half-pins was analysed using FEM to investigate the effects of these pre-tension and half pin on the system stability quantitatively. 3-D finite element models of five different fixator frames were developed using by beam elements. In axial compression analysis, the fracture stiffness was increased maximally 62％ as the pre-tension increased. In torsion analysis, in the other hand, there is little variations in the fracture stiffness. Additional half pin increased the system stiffness about 200 ％. From the results, proper pre-tension and additional half pin would provide good methods to increase the fracture stability of the hybrid external fixator and provide more surgical options to minimize soft tissue damage at the fracture site.

• Korean Journal of Environmental Biology
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• v.26 no.2
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• pp.66-75
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• 2008

The level of genetic diversity and genetic relationships among Korean ring-necked pheasant (Phasianus colchicus karpowi) habitat and subspecies have been investigated based on Inter Simple Sequence Repeat (ISSR) markers. Wild and domesticated Korean ring-necked pheasant, hybrids between domesticated Korean ring-necked and foreign subspecies, and four foreign subspecies; Chinese ring-necked (P. c. torquatus), Melanistic mutant (P. c. mut. tenebrosus), XL White (P. c. mut) and Southern green (P. c. versicolor) were used for comparison. On the basis of the results of AMOV A, 94.08% of genetic diversity in Korean ring-necked was allocated among individuals within habitat differences. Estimate of $\Phi$st, which represents the degree of genetic differentiation among habitats was 5.9%. Based on the dendrogram reconstructed by UPGMA, Yangpyung habitat of the eight habitats turned out to be distinct from others habitat. Interestingly, domesticated Korean ring-necked and hybrid mixture showed closer genetic relationship with four foreign subspecies than Korean ring-necked. As a consequence of AMOVA, 96.63% of genetic diversity in four foreign subspecies was allocated among individuals within subspecies. Estimate of $\Phi$st representing the degree of genetic differentiation among subspecies was 3.4%, which was lower than that among habitats of Korean ring-necked. The lower level of genetic difference among four foreign subspecies showed that these subspecies were genetically closer even though they were morphologically classified into four different subspecies. When seven habitats of Korean ring-necked pheasant and four foreign subspecies were divided into Korean and Foreign Pheasant Groups, respectively, more than 17% of genetic diversity was allocated between groups (about 4% among habitats/subspecies within groups). This observation implied that Korean ring-necked pheasant is genetically quite different from four foreign subspecies. On the basis of cluster analysis, three foreign subspecies (Chinese ring-necked pheasant, Melanistic mutant pheasant, and XL White pheasant) formed a distinct group with domesticated Korean ring-necked pheasant and hybrid mixture at 98% confidence interval.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2002.10a
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• pp.18-25
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• 2002

The pikromycin biosynthetic system in Streptomyces venezuleae is unique for its ability to produce two groups of antibiotics that include the 12-membered ring macrolides methymycin and neomethymycin, and the 14-membered ring macrolides narbomycin and pikromycin. The metabolic pathway also contains two post polyketide-modification enzymes, a glycosyltransferase and P450 hydroxylase that have unusually broad substrate specificities. In order to explore further the substrate flexibility of these enzymes a series of hybrid polyketide synthases were constructed and their metabolic products characterized. The plasmid-based replacement of the multifunctional protein subunits of the pikromycin PKS in S. venezuelae by the corresponding subunits from heterologous modular PKSs resulted in recombinant strains that produce both 12- and 14-membered ring macrolactones with predicted structural alterations. In all cases, novel macrolactones were produced and further modified by the DesVII glycosyltransferase and PikC hydroxylase leading to biologically active macrolide structures. These results demonstrate that hybrid PKSs in S. venezuelae can produce a multiplicity of new macrolactones that are modified further by the highly flexible DesVII glycosyltransferase and PikC hydroxylase tailoring enzymes. This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products. The polyketide backbone of rifamycin B is assembled through successive condensation and ${\beta}$-carbonyl processing of the extender units by the modular rifamycin PKS. The eighth module, in the RifD protein, contains nonfunctional DH domain and functional KR domain, which specify the reduction of the ${\beta}$-carbonyl group resulting in the C-21 bydroxyl of rifamycin B. A four amino acid substitution and one amino acid deletion were introduced in the putative NADPH binding motif in the proposed KR domain encoded by rifD. This strategy of mutation was based on the amino acid sequences of the corresponding motif of the KR domain of module 3 in the RifA protein, which is believed dysfunctional, so as to introduce a minimum alteration and retain the reading frame intact, yet ensure loss of function. The resulting strain produces linear polyketides, from tetraketide to octaketide, which are also produced by a rifD disrupted mutant as a consequence of premature termination of polyketide assembly. Much of the structural diversity within the polyketide superfamily of natural products is due to the ability of PKSs to vary the reduction level of every other alternate carbon atom in the backbone. Thus, the ability to introduce heterologous reductive segments such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER) into modules that naturally lack these activities would increase the power of the combinatorial biosynthetic toolbox. The dehydratase domain of module 7 of the rifamycin PKS, which is predicted to be nonfunctional in view of the sequence of the apparent active site, was replaced with its functional homolog from module 7 of rapamycin-producing polyketide synthase. The resulting mutant strain behaved like a rifC disrupted mutant, i.e., it accumulated the heptaketide intermediate and its precursors. This result points out a major difficulty we have encountered with all the Amycolatopsis mediterranei strain containing hybrid polyketide synthases: all the engineered strains prepared so far accumulate a plethora of products derived from the polyketide chain assembly intermediates as major products instead of just analogs of rifamycin B or its ansamycin precursors.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.21 no.8
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• pp.96-101
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• 2007

Gas Discharge Tubes (GDTs) are widely used as surge protectors for communication applications due to their small internal capacitance. In these days, however, they are mostly used in combined configurations, because the sparkover voltage required to initiate the discharge process in the GDTs and the time taken for arc formation process can be large enough to damage to sensitive circuits. For GDTs with a considerably high initial residual voltage, we should limit the peak voltage using a TVS or filter. We made a hybrid SPD circuits of common-mode type and differential-mode type with the filter using common-mode choke. Also, we applied lightning impulse voltage and ring wave voltage which frequency bandwidth are different each other and verified the characteristics of hybrid SPD circuits according to waveshapes. We describe how the applied SPDs operate in protection process steps with the actual data obtained from the residual voltage measurement at each step. The experiment results show that the surge voltage reduction with the choke coil is more effective in differential-mode circuit than in common-mode circuit.