• IMMUNE NETWORK
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• 제2권3호
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• pp.137-141
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• 2002

Among the members of the inhibitor of apoptosis (IAP) protein family, only Livin and survivin have been reported to have variant forms. We have found a variant form of c-IAP2 through the interaction with the X protein of HBV using the yeast two-hybrid system. In contrast to the wild-type c-IAP2, the variant form has two stretches of sequence in the RING domain that are repeated in the C-terminus that would disrupt the RING domain. We demonstrate that the variant form has an inhibitory effect on TNF-mediated $NF-{\kappa}B$ activation unlike the wild-type c-IAP2, which increases TNFmediated $NF-{\kappa}B$ activation. These results suggest that this variant form has different activities from the wild-type and the RING domain may be involved in the regulation of TNF-induced $NF-{\kappa}B$ activation.

• 한국통신학회논문지
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• 제31권12A호
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• pp.1177-1181
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• 2006

본 논문에서는 링 레이저를 위한 새로운 광대역 파장 가변 필터를 제안하였다. 두 종류의 필터를 제안하였는데, Fiber Reflection Mach-Zehnder Interferometer와 Sagnac필터로 구성된 것과 하이브리드 필터와 Fiber Reflection Mach-Zehnder Interferometer로 구성된 것이다. 시뮬레이션을 통해 50nm의 연속적인 파장 가변을 얻을 수 있음을 알 수 있었고, 기존의 필터에 비해 파장 가변 범위가 3배에 이르면서도 FWHM이 1/3로 줄어든 통과 스펙트럼 특성을 얻을 수 있었다. 이는 링 레이저의 광대력 파장 가변 및 안정적인 단일모드 발진을 더욱 가능케 하는 중요한 특성이다.

• 제어로봇시스템학회논문지
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• 제19권11호
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• pp.960-967
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• 2013

Shape recognition of solder ball bumps in a BGA (Ball Grid Array) is an important issue in flip chip bonding technology. In particular, the semiconductor industry has required faster and more accurate inspection of micron-size solder bumps in flip chip bonding as the density of balls has increased dramatically. The difficulty of this issue comes from specular reflection on the metal ball. Shape recognition of a metal ball is a very realproblem for computer vision systems. Specular reflection of the metal ball appears, disappears, or changes its image abruptly due to tiny movementson behalf of the viewer. This paper presents a practical shape recognition method for three dimensional (3-D) inspection of a BGA using a 5-step ring illumination device. When the ring light illuminates the balls, distinctive specularity images of the balls, which are referred to as "iso-slope contours" in this paper, are shown. By using a mathematical reflectance model, we can drive the 3-D shape information of the ball in aquantitative manner. The experimental results show the usefulness of the method for industrial application in terms of time and accuracy.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.226-231
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• 2004

Press fitting method for joining of a hybrid tube and steel ring with small teeth for automotive aluminum/composite hybrid propeller shaft was devised to improve reliability and to reduce manufacturing cost, compared to other joining methods such as an adhesively bonded joint, bolted joint or welded joint. To obtain high strength of the press fit joint, an optimal design method for the teeth was devised with respect to number and shape of the steel teeth. Torsional static, fatigue tests and finite element analysis of the press fit joint were performed with respect to experimental variables. The developed optimal design method predicted well the static torque capability and failure mode of the press fit joint. Also, it provided design guide line of press fit joint for improving torsional static and fatigue characteristics.

• International Journal of Precision Engineering and Manufacturing
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• 제2권2호
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• pp.26-30
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• 2001

This paper deals with the feasibility assessment of hybrid linear motor that operates based on self-moving cell concept. The moving cell is composed of Magnetostrictive actuator and a ring structure, and a cell train is constructed by connecting two cells in series. Since this motor uses strong push force of Terfenol-D actuators and friction of the cells, it can essentially produce long stroke and large force. The overall performance of the motor was measured in terms of speed and force.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2006년도 추계 학술발표회 논문집
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• pp.253-256
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• 2006

The HPFRCCs show the multiple crack and damage tolerance capacity due to the interfacial bonding of the fibers to the cement matrix. For practical application, it is needed to investigate the fractural behavior and of HPFRCCs and understand the micro-mechanism of cement matrix with reinforcing fiber. The objectives of this paper are to examine the compressive behavior, fracture and damage process of HPFRCC by acoustic emission technique. Total four series were tested, and the main variables were the hybrid type, polyethylene (PE) and steel cord (SC), and fiber volume fraction. The damage progress by compressive behavior of the HPFRCCs is characteristic for the hybrid fiber type and volume fraction. And from acoustic emission (AE) parameter value, it is found that the second and third compressive load cycles resulted in successive decrease of the ring-down count rate as compared with the first compressive load cycle.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 추계학술대회 논문집
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• pp.389-392
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• 2004

It is clinically well known that pre-tension of wires increases the fracture stability in ring or hybrid external fixation. In some cases, additional half pin should be necessary to increase the stability when soft tissue impalement occurs during fixation. In this paper, the fracture stability of a hybrid external fixator system with different pre-tension effects and additional half-pins was analysed using FEM to investigate the effects of these pre-tension and half pin on the system stability quantitatively. 3-D finite element models of five different fixator frames were developed using by beam elements. In axial compression analysis, the fracture stiffness was increased maximally 62％ as the pre-tension increased. In torsion analysis, in the other hand, there is little variations in the fracture stiffness. Additional half pin increased the system stiffness about 200 ％. From the results, proper pre-tension and additional half pin would provide good methods to increase the fracture stability of the hybrid external fixator and provide more surgical options to minimize soft tissue damage at the fracture site.

• 환경생물
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• 제26권2호
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• pp.66-75
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• 2008

한국꿩 (Korean ring-necked Pheasant, Phasianus colchicus karpowi)과 외국 아종의 유전적 유연관계를 파악하기 위해 야생 한국꿩, 사육 한국꿩, 사육 한국꿩과 외국꿩간의 잡종꿩, 외국꿩 4아종(중국 링넥, 흑 뮤탄트, 백 뮤탄트, 녹치)을 대상으로 ISSR 표지자 분석과 AMOVA 분석을 수행하였다. 야생 한국꿩의 전체 유전 다양성중 94.08%가 서식지 내 개체간 유전적 차이에 기인하고, 5.9% ($\Phi$st=0.059)가 서식지간 차이에 기인하였다. 사육 한국꿩이 유전적으로 야생 한국꿩 보다는 외국꿩 4아종과 가깝게 나타났다. AMOVA분석과 cluster분석에서 사육 한국꿩이 야생 한국꿩은 물론 외국꿩 4아종과도 유전적 차이를 보이는 것으로 미루어 볼 때 사육되고 있는 한국꿩은 한국꿩과 외국 아종간의 다양한 교잡에 의해 생겨난 잡종일 가능성이 높다. 외국꿩 4아종(중국 링넥, 흑 뮤탄트, 백 뮤탄트, 녹치)의 전체 유전 다양성중 96.63%가 아종내 개체간 차이에 기인하고. 3.4% ($\Phi$st=0.034)로 아종간 차이에 기인하여 외국꿩 4아종의 아종간 유전적 차이가 야생 한국꿩의 서식지간 차이보다도 낮은 것으로 나타났다. 이는 본 분석에 사용된 외국꿩 4아종이 형태적으로는 다른 아종으로 분류되지만 유전적으로는 매우 가까운 위치에 있음을 의미한다. 7서식지의 야생 한국정과 외국꿩 4아종을 각각 야생 한국꿩 그룹과 외국꿩 그룹으로 분류하여 두 그룹의 유전적 다양성을 분석한 결과, 전체 유전변이 중 그룹간 차이의 비율은 17% 이상(그룹 내 서식지/아종간 차이는 약 4%)으로 야생 한국꿩이 아종 관계인 외국꿩 4아종과 상당한 유전적 차이를 보이는 것으로 나타났다. 유전적 유연관계 분석결과에서 중국 링넥. 흑 뮤탄트, 백 뮤탄트의 외국꿩 3 아종은 유전적으로 외국 아종에 가까운 사육한국꿩, 잡종꿩과 함께 하나의 분지군을 형성하면서 야생 한국꿩 그룹으로부터 98% 신뢰수준에서 뚜렷하게 구별되었다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2002년도 학술발표대회
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• pp.18-25
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• 2002

The pikromycin biosynthetic system in Streptomyces venezuleae is unique for its ability to produce two groups of antibiotics that include the 12-membered ring macrolides methymycin and neomethymycin, and the 14-membered ring macrolides narbomycin and pikromycin. The metabolic pathway also contains two post polyketide-modification enzymes, a glycosyltransferase and P450 hydroxylase that have unusually broad substrate specificities. In order to explore further the substrate flexibility of these enzymes a series of hybrid polyketide synthases were constructed and their metabolic products characterized. The plasmid-based replacement of the multifunctional protein subunits of the pikromycin PKS in S. venezuelae by the corresponding subunits from heterologous modular PKSs resulted in recombinant strains that produce both 12- and 14-membered ring macrolactones with predicted structural alterations. In all cases, novel macrolactones were produced and further modified by the DesVII glycosyltransferase and PikC hydroxylase leading to biologically active macrolide structures. These results demonstrate that hybrid PKSs in S. venezuelae can produce a multiplicity of new macrolactones that are modified further by the highly flexible DesVII glycosyltransferase and PikC hydroxylase tailoring enzymes. This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products. The polyketide backbone of rifamycin B is assembled through successive condensation and ${\beta}$-carbonyl processing of the extender units by the modular rifamycin PKS. The eighth module, in the RifD protein, contains nonfunctional DH domain and functional KR domain, which specify the reduction of the ${\beta}$-carbonyl group resulting in the C-21 bydroxyl of rifamycin B. A four amino acid substitution and one amino acid deletion were introduced in the putative NADPH binding motif in the proposed KR domain encoded by rifD. This strategy of mutation was based on the amino acid sequences of the corresponding motif of the KR domain of module 3 in the RifA protein, which is believed dysfunctional, so as to introduce a minimum alteration and retain the reading frame intact, yet ensure loss of function. The resulting strain produces linear polyketides, from tetraketide to octaketide, which are also produced by a rifD disrupted mutant as a consequence of premature termination of polyketide assembly. Much of the structural diversity within the polyketide superfamily of natural products is due to the ability of PKSs to vary the reduction level of every other alternate carbon atom in the backbone. Thus, the ability to introduce heterologous reductive segments such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER) into modules that naturally lack these activities would increase the power of the combinatorial biosynthetic toolbox. The dehydratase domain of module 7 of the rifamycin PKS, which is predicted to be nonfunctional in view of the sequence of the apparent active site, was replaced with its functional homolog from module 7 of rapamycin-producing polyketide synthase. The resulting mutant strain behaved like a rifC disrupted mutant, i.e., it accumulated the heptaketide intermediate and its precursors. This result points out a major difficulty we have encountered with all the Amycolatopsis mediterranei strain containing hybrid polyketide synthases: all the engineered strains prepared so far accumulate a plethora of products derived from the polyketide chain assembly intermediates as major products instead of just analogs of rifamycin B or its ansamycin precursors.

• 조명전기설비학회논문지
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• 제21권8호
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• pp.96-101
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• 2007

가스방전관(GDT)은 내부 정전용량이 작아서 통신용 서지보호기로 널리 사용되고 있는 소자이다. 하지만 가스방 전관의 동작개시를 위해서는 방전이 개시될 수 있는 충분한 전압과 시간이 필요하며, 이 동작개시전압은 피보호 회로가 서지에 민감한 장비일 경우 손상을 줄 수 있을 만큼 크므로 최근에는 조합 형태로 적용하는 경우가 대부분이다. 이렇게 초기 과전압이 상당 부분 존재하는 가스방전관에는 추가적으로 TVS나 필터 등을 통해 피크 전압을 제한해 주어야 한다. 본 논문에서는 공통모드 초크코일을 이용한 필터를 적용하여 공통모드와 차동모드의 조합회로를 구성하고, 주파수 대역이 다른 뇌임펄스 전압과 ring wave 전압을 인가하여 파형에 따른 조합회로의 특성을 확인하였다. 다단으로 보호되는 과정을 각각의 측정점에서 잔류전압을 측정하여 단계별로 적용한 SPD들이 어떻게 동작하는지를 실측데이터로 제시하였다. 각 단계의 잔류전압을 비교했을 때 공통모드에 비하여 차동모드에서 서지전압을 제한하는 성능이 보다 효과적이다.