• IMMUNE NETWORK
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• v.16 no.4
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• pp.249-255
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• 2016

Exogenous nucleic acids induce an innate immune response in mammalian host cells through activation of the retinoic acid-inducible gene I (RIG-I). We evaluated RIG-I protein for RNA binding and ATPase stimulation with RNA ligands to investigate the correlation with the extent of immune response through RIG-I activation in cells. RIG-I protein favored blunt-ended, double-stranded RNA (dsRNA) ligands over sticky-ended dsRNA. Moreover, the presence of the 5'-triphosphate (5'-ppp) moiety in dsRNA further enhanced binding affinity to RIG-I. Two structural motifs in RNA, blunt ends in dsRNA and 5'-ppp, stimulated the ATP hydrolysis activity of RIG-I. These structural motifs also strongly induced IFN expression as an innate immune response in cells. Therefore, we suggest that IFN induction through RIG-I activation is mainly determined by structural motifs in dsRNA that increase its affinity for RIG-I protein and stimulate ATPase activity in RIG-I.

• International Journal of Naval Architecture and Ocean Engineering
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• v.11 no.2
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• pp.648-661
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• 2019

Thin fabric-based yacht sails have a cambered shape to generate lift force; however, their shape can be easily deformed by wind pressure and also affected by the deformation of the mast. These deformations can change the airflow characteristics over the sail. Therefore, Fluid-Structure Interaction (FSI) analysis is needed to evaluate the sail force precisely. In this study, airflow over the deformed sail and rig was studied using FSI. Elastic deformation of the sail and rig was obtained by an aerodynamic calculation under dynamic pressure loading on the sail surface. The effects of rig deformation on the aerodynamic performance of the sail were examined according to the rig type and mast flexibilities. As a result, the changes of lift force for a fractional type rig with a thin mast section were more significant than with a masthead rig.

• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.2014-2021
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• 2019

Middle East respiratory syndrome coronavirus (MERS-CoV) belongs to the genus Betacoronavirus and causes severe morbidity and mortality in humans especially when infected patients have underlying diseases such as chronic obstructive pulmonary disease (COPD). Previously, we demonstrated that MERS-CoV-encoded ORF8b strongly inhibits MDA5- and RIG-I-mediated induction of the interferon beta (IFN-β) promoter activities. Here, we report that ORF8b seemed to regulate MDA5 or RIG-I differentially as protein levels of MDA5 were significantly down-regulated while those of RIG-I were largely unperturbed. In addition, ORF8b seemed to efficiently suppress phosphorylation of IRF3 at the residues of 386 and 396 in cells transfected with RIG-I while total endogenous levels of IRF3 remained largely unchanged. Furthermore, ORF8b was able to inhibit all forms of RIG-I; full-length, RIG-I-1-734, and RIG-I-1-228, the last of which contains only the CARD domains. Taken together, it is tempting to postulate that ORF8b may interfere with the CARD-CARD interactions between RIG-I and MAVS. Further detailed analysis is required to delineate the mechanisms of how ORF8b inhibits the MDA5/RIG-I receptor signaling pathway.

• Journal of the Korea Society for Simulation
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• v.20 no.2
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• pp.11-17
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• 2011

The purpose of this study is 3 dimensional modeling of lower part of drilling system in offshore rig and simulation of drilling process. Recently, shipbuilding companies have been focusing on offshore rigs due to their high added-value and the reduced demand of new shipbuilding. In most cases, however, the basic design, installation and management of offshore rig are carried out by foreign companies. Therefore, it is difficult to obtain the knowledge and information of drilling system. In this study drilling devices, BOP(Blowout Preventor) and cementing job and mud circulation related components are included as the main components of offshore rig. The structure and function of them were analyzed from a viewpoint of object-oriented technique. On the basis of this analysis they were modeled in the 3 dimensional structure with 3D software tool such as CATIA and 3DVIA Composer. The drilling process was simulated according to the scenario of drilling operation. This simulation system can be effectively used for an educational tool for students and engineers in ocean plant industries.

• Proceedings of the KSME Conference
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• 2007.05a
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• pp.1207-1212
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• 2007

The critical speed of railway bogie related to the stability of the railway rolling-stock is important. Testing of the dynamic performance of bogie is conducted using a roller rig in a laboratory in place of field testing on track. This roller rig is composed of two rollers equivalent to track and used to test the dynamic characteristics of vehicle. But, the geometrical characteristics of the wheel/roller contact on the roller rig are different from those of the wheel/rail contact because the longitudinal radius of roller is not infinite compared with rail. This difference has influence on the wheelset behavior and the critical speed of bogie. Therefore in this paper, we have studied the behavior of wheelset and bogie on the roller rig for railway bogie testing with the purpose of developing the scaled roller rig. As an analysis results, it has been shown that the critical speed of bogie on the roller rig is slightly lower than that of bogie on track.

• Journal of the Korean Society for Railway
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• v.8 no.1
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• pp.101-109
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• 2005

Roller test rig is the test facility for acquiring the data related dynamic characteristics of rolling stocks handily. The test method using roller test rig could be convenient to Set information about hunting and others rather than using field test. However, adopting this method brings about the inconvenient calibration process for measured data. This kind of additional effort results from the difference of contact condition between field test and test roller rig. In this paper, the numerical approach is used to get the amount of calibration. The analysis results of the field test are compared with results of the roller test rig to know what kind of alternation happens according to the change of contact condition.

• Journal of the KSME
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• v.57 no.11
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• pp.30-32
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• 2017

이 글에서는 한전 전력연구원에서 보유한 열충격 시험설비 ACRT(Automatic Combustion Rig Tester)와 Burner Rig Test Unit를 소개하고자 한다.

• Journal of Advanced Marine Engineering and Technology
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• v.36 no.8
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• pp.1136-1142
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• 2012

With concern for new renewable energy sources rising, the offshore wind turbine energy market is growing. In order to install offshore wind turbines safely in a harsh environment, jack-up rigs need to be used. Recently, a WTIV(Wind Turbine Installation Vessel), which has a self-propelling system, was developed to replace traditional jack-up rigs. Since the jack-up rig works at 60 meters of depth in offshore and the seafloor is composed mostly of soft clay and sand, it is necessary to conduct a stability examination for ground subsidence before using it. This study conducts an improved numerical analysis of the effect of jack-up rig loading on the soft ground by using FLAC3D considering consolidation theory. This includes analyzing the amount of subsidence when applying different cases of surchargings. It can be concluded that the mid-loading method has an advantage as regards clay layers and the pre-loading method has an advantage as regards sand layers.

• International Journal of Automotive Technology
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• v.7 no.4
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• pp.441-448
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• 2006

In-cylinder flows such as tumble and swirl play an important role on the engine combustion efficiencies and emission formations. The tumble flow, which is dominant in current high performance gasoline engines, is able to effect fuel consumptions and emissions under a partial load condition in addition to the volumetric efficiency under a wide open throttle condition. Therefore, it is important to optimize the tumble ratio of a gasoline engine for better fuel economy, lower emissions, and maximum volumetric efficiency. First step for optimizing a tumble ratio is to measure a tumble ratio accurately. For a tumble ratio measurement, many different methods have been developed and used such as steady flow rig, PIV, PTV, and LDV. However, it is not well known about the relations among the measured tumble ratios using different methods. The purpose of this research is to correlate the tumble ratios measured using three different methods and find out merits and demerits of each measurement method. In this research the tumble flow was measured, compared, and correlated using three different measurement methods at the same engine: steady flow rig; 2-dimensional PIV; and 3-dimensional PTV water flow rig.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.227-238
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• 1993

IRRI Engineering Division has developed a well drilling rig attachment that matched power tiller or hand tractor. It was designed in response to the growing demand for ground water utilization for small-scale irrigation, especially in drought-sticken and rained farms in Asian countries. The power tiller-driven rig can drill 30 meters of 100mm well in an unconsolidated formation in one day and can be rapidly converted from rotary to jetting or to the percussion method of drilling to suit different soil and rock formation. In addition, the power tiller can be quickly installed or removed from the rig frame and can be used for transporting the rig to other sites. The rig can be dismantled into smaller sub-assemblies for carrying by hand into less accessible areas. One manufacturer in Central luzon Philippines has started to produce the rig for well drillers in Central Luzon. The Department of Agriculture in the Philippines have procured thirty three(33) units of these machines f r their Shallow Tubewell program.