• International Journal of Industrial Entomology and Biomaterials
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• v.14 no.1
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• pp.9-13
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• 2007

Hexane extract of Porteresia coarctata (Roxb.) exhibits a toxic effect on the tissues of Spodoptera litura (F) while fed at the dose of 1000 and 2000 ppm thoroughly mixing with castor leaves (Ricinus communis L) after dissolving in DMSO at late fourth instar whereas only DMSO treated castor leaves were fed to control group. The larvae were put to rear at $28^{\circ}C{\pm}1^{\circ}C$, $76{\pm}4%$ R.H. under 12 L + 12 D photoperiodic regime. In test group insects substantial reduction of protein and DNA content was marked in fat body and midgut tissues compared to DMSO treated control group. The significant biochemical alterations in the midgut tissues and fat body of test group insects indicate the insecticidal property of the said plant extract that could be tested in facilitating the phenomenal stride in Integrated Pest Management.

• Korean Journal of Plant Resources
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• v.22 no.1
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• pp.1-4
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• 2009

This study was carried out to evaluate ecological growth characteristics of forty castor bean collections for biodiesel utilization. The emergence date and it's rate of collections was 15-16 days and 82.5%, respectively. It took 63-68 days from emergence to flowering. Stem length was ranged from 173 to 251 cm with an average 211.8 cm. The average stem diameter was 24.2 cm, and showed the high occurrence as 52.5% at the 20.4-24.9 cm. The node number was widely distributed as 13-22 and the average node number was 18.9. The seed numbers were widely distributed as 7-33ea, and the average liter weight of seed was 502.9 g of which minimum weight was 432.3 g and maximum weight was 572.5 g, and the gap between maximum and minimum was 140.2 g. It showed high difference. The 100 grain weight was distributed as 20.3 g-38.3 g which showed high differences between small grain and large grain collections. The seed numbers per plant were varied as 72 at the minimum and 1,011 at the maximum number, and average seed number was 465.2. The total yields of seed per plant were varied as 20 g-266.2 g, and the average yields were 128.1 g.

• Journal of the korean society of oceanography
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• v.34 no.3
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• pp.167-171
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• 1999

Since toxic Alexandrium catenella and non-toxic A. fraterculus are morphologically similar, they are difficult to discriminate under the light microscope. However, a novel technology, such as fluorescein isothiocyanate (FITC)-conjugated lectin probes enables easy and rapid differentiation. Toxic A. catenella bound seven different lectins, whereas the non-toxic A. fratercuzus did not bind Arachis hypogaea (PNA) lectin. In addition, Pseudo-nitrschia species in this study were also difficult to identify to species level with light microscope techniques, but it was possible to classify them using fluorescent lectins. Pseudo-nitzschia multistriata, P. subfraudulenta and P. pungens bound Canavalia ensiformis (ConA), whereas P. subpaclfica did not, and P. pungens also bound Ricinus communis (RCA). These results imply that lectin could be used as a critical tool in the differentiation of P. multistriata, P. subfraudulenta and P. pungens. However, P. subpacifica was not differentiated by the lectins tested. Therefore, it isconcluded that lectin probes are useful for discriminating toxic A. catenella from non-toxic A. fraterculus, and for the identification of some Pseudo-nitzschia species. In addition, this method has a great potential to speed and detection between non-toxic and toxic harmful algal blooms (HABs) in Korean biotoxin monitoring systems.

• BMB Reports
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• v.57 no.2
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• pp.86-91
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• 2024

The fatty acids content of castor (Ricinus communis L.) seed oil is 80-90% ricinoleic acid, which is a hydroxy fatty acid (HFA). The structures and functional groups of HFAs are different from those of common fatty acids and are useful for various industrial applications. However, castor seeds contain the toxin ricin and an allergenic protein, which limit their cultivation. Accordingly, many researchers are conducting studies to enhance the production of HFAs in Arabidopsis thaliana, a model plant for oil crops. Oleate 12-hydroxylase from castor (RcFAH12), which synthesizes HFA (18:1-OH), was transformed into an Arabidopsis fae1 mutant, resulting in the CL37 line producing a maximum of 17% HFA content. In addition, castor phospholipid:diacylglycerol acyltransferase 1-2 (RcPDAT1-2), which catalyzes the production of triacylglycerol by transferring HFA from phosphatidylcholine to diacylglycerol, was transformed into the CL37 line to develop a P327 line that produces 25% HFA. In this study, we investigated changes in HFA content when endogenous Arabidopsis PDAT1 (AtPDAT1) of the P327 line was edited using the CRISPR/Cas9 technique. The successful mutation resulted in three independent lines with different mutation patterns, which were transmitted until the T4 generation. Fatty acid analysis of the seeds showed that HFA content decreased in all three mutant lines. These findings indicate that AtPDAT1 as well as RcPDAT1-2 in the P327 line are involved in transferring and increasing HFAs to triacylglycerol.

• BMB Reports
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• v.40 no.4
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• pp.595-603
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• 2007

To investigate whether phospholipase D (PLD, EC 3.1.4.4) plays a role in adaptive response of post-harvest fruit to environment, a PLD gene was firstly cloned from grape berry (Vitis Vinifera L. cv. Chardonnay) using RT-PCR and 3'- and 5'-RACE. The deduced amino acid sequence (809 residues) showed 84.7% identity with that of PLD from Ricinus communis. The secondary structures of this protein showed the characteristic C2 domain and two active sites of a phospholipid-metabolizing enzyme. The PLD activity and its expression in response to heat acclimation were then assayed. The results indicated PLD was significantly activated at enzyme activity, as well as accumulation of PLD mRNA and synthesis of new PLD protein during the early of heat acclimation, primary suggesting that the grape berry PLD may be involved in the heat response in post-harvest grape berry. This work offers an important basis for further investigating the mechanism of post-harvest fruit adaptation to environmental stresses.

• Korean journal of applied entomology
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• v.52 no.2
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• pp.93-100
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• 2013

In this study, the distribution of Cantao ocellatus in Korea occurred at Jindo, Tongyoung, Gwangju and Taean including Jejudo in 2012 and was coincidentally in accordance with the distribution of Mallotus japonicus as a host plant. The adult emerges in M. japonicus occur from late June to early July and can be observed until the end of October. The investigation of the female and male was measured, developmental characteristics, host plant and oviposition preference under the conditions of temperature $25^{\circ}C({\pm}2)$, humidity 65%(${\pm}2$), day length 16L:8D, by indoor breeding. For females and males respectively, average body lengths were 26.20 mm and 23.88 mm, body widths 11.35 mm and 10.57 mm, head widths 3.84 mm and 3.64 mm, probosics lengths 7.90 mm and 7.27 mm, antennal lengths 9.87 mm and 9.69 mm, anterior leg lengths 12.50 mm and 12.27 mm, intermediate leg lengths 14.61 mm and 13.12 mm, posterior leg lengths 16.90 mm and 16.53 mm, and fresh weights 0.46 g and 0.31 g. It was seen that two kinds of C. ocellatus had prickles at the end of the pronotum which had developed in the fifth instars. The female is distinguished from the male by the reproductive organ and the spotted pattern on the abdominal segment. The preference of drinks was fruit, leafstalk, midrib, and branch in Mallotus japonicus (Thunb.) Muell. Arg., Mallotus japonicus 'Variegatus', Ricinus communis L., Lonicera japonicus var. repens (Siebold) Rehder, Citrus sinensis (L.) Osbeck and Zelkova serrata (Thunb.) Makino.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.10
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• pp.1308-1318
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• 2010

The purpose of this study was to evaluate the effect of replacing soybean meal with treated castor meal with (CMT) or without lime (CMNT) on the nutrient intake, performance, carcass characteristics, and yield of commercial cuts of beef cattle from a feedlot. Thirty male, castrated, crossbreed zebu cattle were used in the study, with an average initial weight of $360{\pm}30.27\;kg$. Five animals were used as a control group and were slaughtered at the beginning of the experiment; the remaining animals (n = 25) were distributed in random blocks (repetitions), with body weight as the criterion for block assignment. The animals were fed a diet containing 65% corn silage and 35% of concentrate on dry matter (DM) basis. Five diets consisted of four levels of soybean meal (SM) substituted with CMT (0, 33, 67 and 100%) on a DM basis and a diet with 100% of SM replaced with CMNT. At the end of the experiment, all animals were slaughtered, and their gastrointestinal tracts were emptied to determine their empty body weights (EBW). No significant effects were observed (p>0.05) for the substitution of soybean meal with CMT on intake of dietary nutrients, the average daily body weight gain (ADG) or EBW gain (EBWG). In spite of greater (p<0.05) ricin intake for the diet containing CMNT (3.06 mg/kg BW) compared to the CMT diet (0.10 mg/kg BW/d), there were no effects (p>0.05) on intake of dietary nutrients, ADG or EBWG. The average intake of DM and the ADG were 10,664.63 and 1,353.04 g/d, respectively. Regarding carcass characteristics, only carcass yield in relation to body weight was linearly reduced (p<0.05) upon substitution of SM by CMT. There was no effect (p>0.05) of the substitution of SM by CMT or CMNT on the yield of carcass basic cuts. CMT prices that are higher than 85% of the SM price do not economically justify the use of CMT. For CMT prices between 20 and 80% of the SM price, the optimal level was 67% substitution, while for prices below 15% of the SM price, the optimal level was 100% substitution with CMT. It can be concluded that treated castor meal with 6% lime can totally replace soybean meal in beef cattle diets.

• Clinical and Experimental Reproductive Medicine
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• v.29 no.3
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• pp.179-186
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• 2002

Objective: Lectins are cell-agglutinating and sugar specific proteins or glycoproteins of non-immune origin that precipitate glycoconjugates having saccharides of appropriate complementarity. Because of these properties, plant lectins have been used to help characterize the carbohydrate moieties of glycoproteins in the zona pellucida (ZP) of several mammalian species including pigs. Treatment of oocytes with various lectins blocks sperm binding to the ZP in various mammalian species. This study was undertaken to examine the distribution of sugar residues in the ZP of pig oocytes matured in vitro and the ability of spermatozoa to bind to ZP and in vitro penetration in oocytes treated with fluorescein isothiocyanate (FITC)-labelled lectins. Materials and Methods: The lectins of Banderiaea simplicifolia (BS-II, bind to $\beta$-D-N-acetylglucosamine), Canavalin ensiformis (Con A, bind to $\alpha$-D-Mannose), Lens culinaris (LCA, bind to a-D-Mannose), Ricinus communis (RCA-I, bind to $\beta$-D-Galactose) and Ulex europaeus (UEA-I, bind to $\alpha$-L-Fucose) were examined for spermatozoa penetration, binding capacity to ZP and distribution of lectins. Results: The penetration rates were significantry (p<0.05) higher in control oocytes (63%) than those treated with all lectins, but penetration rates ($40{\sim}49%$) were simililar in group treated with lectins. The incidence of monospermy was similar in oocytes untreated and UEA-I, but it was higher in oocytes treated with BS-II, Con A, RCA-I and LCA. The porcine oocytes cultured for 48 h in TC-199 medium were freed from cumulus cells and treated for 30 min with fluorescein isothiocyanate-labelled lectins. When examined under fluorescein illumination, higher (p<0.001) proportions of oocytes showed fluorescein of zona pellucida after treatment with Con A (93%), LCA (93%) and RCA-I (100%) than BS-II (37%) and UEA-I (50%). All of the oocytes treated with RCA-I exhibited strong fluorescein in the outer region of the zona pellucida while those treated with LCA exhibited strong fluorescein throughout the zona pellucida. BS-II bounded mainly to the outer region and UEA-I bounded mainly to the inner region of the zona pellucida, with either strong or weak fluorescein. At 120 min after insemination in vitro, fewer spermatozoa were bound to the zona pellucida of the oocytes treated with BS-II, Con-A and RCA-I. Of the lectins, Con A most inhibited sperm binding. Conclusions: These results suggest that $\beta$-D-Galactose residues in the porcine zona pellucida may act as primary sperm receptors and inducers of the sperm acrosome reaction and these sugar residues may be involved in the block to polyspermy.

• Research in Plant Disease
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• v.10 no.2
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• pp.100-104
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• 2004

Two species of Phytophthora were isolated from a leaf blight disease found on castor bean plants growing at a residential area of Hwanggeum-dong in Daegu city. One species was producing conspicuously papillate, noncaducous, ovoid to obpyriform sporangia, 31.2-58.5 ${\times}$ 25.4-44.1 ${\mu}{\textrm}{m}$ in range with an average of 46.4 $\pm$ 6.5 ${\times}$ 35.9 $\pm$ 4.7 ${\mu}{\textrm}{m}$ on simple sympodial sporangiophores, was heterothallic with oogonia globose, 22.5-35.0 ${\mu}{\textrm}{m}$ with an average of 30 $\pm$ 3.3 ${\mu}{\textrm}{m}$ in diameter, oospores plerotic, 18.8-30.0 ${\mu}{\textrm}{m}$ in range averaging 25.4 $\pm$ 2.8 ${\mu}{\textrm}{m}$, and with antheridia amphigynous. This species was identified as P. nicotianae Breda de Haan. The other species produced papillate, caducous, ovoid to ellipsoid sporangia with pedicel, 21.5-54.6 ${\times}$ 17.6-34.3 ${\mu}{\textrm}{m}$ in range with an average of 41.7 $\pm$ 7.1 ${\times}$ 28.4 $\pm$ 4.0 ${\mu}{\textrm}{m}$, and was heterothallic with oogonia globose, 21.3-26.8 ${\mu}{\textrm}{m}$ with an average of 23.0 $\pm$ 1.6 ${\mu}{\textrm}{m}$ in diameter, oospores plerotic, 17.5-23.8 ${\mu}{\textrm}{m}$ in range averaging 20.2 $\pm$ 1.8 ${\mu}{\textrm}{m}$, and with antheridia amphigynous. This species was identified as P. palmivora (Butler) Butler. Both species produced chlamydospores. Pathogenicity of the 2 speices was confirmed by foliar inoculation of castor bean seedlings. Both species have been reported to infect castor bean plants in a few foreign countries but it is the first that a disease of castor bean plants caused by either or both of the species was found in Korea.