• The Plant Pathology Journal
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• 제36권3호
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• pp.231-243
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• 2020

Rice blast, caused by Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. The aim of this study was to screen bacterial isolates to efficiently prevent the occurrence of rice blast. A total of 232 bacterial isolates were extracted from nonrhizospheric rice soil and were screened for antifungal activity against M. oryzae using a leaf segment assay. Strains S170 and S9 showed significant antagonistic activity against M. oryzae in vitro and in leaf disk assays, and controlled M. oryzae infection under greenhouse conditions. The results showed that strains S170 and S9 could effectively control rice leaf blast and panicle neck blast after five spray treatments in field. This suggested that the bacterial strains S170 and S9 were valuable and promising for the biocontrol of rice disease caused by M. oryzae. Based on 16S rDNA, and gyrA and gyrB gene sequence analyses, S170 and S9 were identified as Bacillus amyloliquefaciens and B. pumilus, respectively. The research also demonstrated that B. amyloliquefaciens S170 and B. pumilus S9 could colonize rice plants to prevent pathogenic infection and evidently suppressed plant disease caused by 11 other plant pathogenic fungi. This is the first study to demonstrate that B. amyloliquefaciens and B. pumilus isolated from nonrhizospheric rice soil are capable of recolonizing internal rice stem tissues.

• 한국작물학회지
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• 제41권3호
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• pp.370-375
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• 1996

포승통의 미사질식양토인 간척지에서 벼 건답직파재배 가능성을 구명하고자 서안벼를 공시하고, 간이온실에서는 시용 및짚의 양과 토양수분조건을 달리 하여 파종심도별 벼의 출아율을 조사하고, 실제 포장 시험에서는 산파후 로타리 복토 유무에 따른 벼의 입모 및 수량성의 차리를 비교 검토한 결과는 다음과 같다. 1. 볏짚 처리(특히, 4ton, 6ton/ha)가 무처리보다 출아율이 높았다. 2. 헥타르 당 볏짚 4ton을 처리하고 파종심도가 얕을수록 출아율이 높았으며 초장도 현저히 커졌다. 3. 토양수분의 적습조건은 과습조건보다 출아율이 높았으며, 초장은 적습조건에서 파종심도가 얕을수록 커졌다. 4. 산파후 로타리 복토유무별 입모수는 로타리무복토(73개 /m$^2$)가 로타리복토(44개 /m$^2$)보다 많았다. 5. m$^2$ 당 수수는 로타리무복토가 로타리복토보다 많았고, 수량도 같은 경향을 보였다.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1292-1298
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• 2022

Soil autotrophic bacterial communities play a significant role in the soil carbon (C) cycle in paddy fields, but little is known about how rhizosphere soil microorganisms respond to different long-term (35 years) fertilization practices under double rice cropping ecosystems in southern China. Here, we investigated the variation characteristics of rhizosphere soil RubisCO gene cbbL in the double rice ecosystems of in southern China where such fertilization practices are used. For this experiment we set up the following fertilizer regime: without any fertilizer input as a control (CK), inorganic fertilizer (MF), straw returning (RF), and organic and inorganic fertilizer (OM). We found that abundances of cbbL, 16S rRNA genes and RubisCO activity in rhizosphere soil with OM, RF and MF treatments were significantly higher than that of CK treatment. The abundances of cbbL and 16S rRNA genes in rhizosphere soil with OM treatment were 5.46 and 3.64 times higher than that of CK treatment, respectively. Rhizosphere soil RubisCO activity with OM and RF treatments increased by 50.56 and 45.22%, compared to CK treatment. Shannon and Chao1 indices for rhizosphere soil cbbL libraries with RF and OM treatments increased by 44.28, 28.56, 29.60, and 23.13% compared to CK treatment. Rhizosphere soil cbbL sequences with MF, RF and OM treatments mainly belonged to Variovorax paradoxus, uncultured proteobacterium, Ralstonia pickettii, Thermononospora curvata, and Azoarcus sp.KH33C. Meanwhile, cbbL-carrying bacterial composition was obviously influenced by soil bulk density, rhizosphere soil dissolved organic C, soil organic C, and microbial biomass C contents. Fertilizer practices were the principal factor influencing rhizosphere soil cbbL-carrying bacterial communities. These results showed that rhizosphere soil autotrophic bacterial communities were significantly changed under conditions of different long-term fertilization practices Therefore, increasing rhizosphere soil autotrophic bacteria community with crop residue and organic manure practices was found to be beneficial for management of double rice ecosystems in southern China.

• 한국토양비료학회지
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• 제46권3호
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• pp.187-192
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• 2013

This study was carried out to investigate the incorporation effect of green manure crops (GMC) such as the hairy vetch on improvement of soil environment in reclaimed land during silage corn cultivation over the past two years. Plots consisted of conventional fertilization (CF) and incorporation of GMC were divided by addition rate of nitrogen fertilizer (100 kg $ha^{-1}$) with 30 - 100% of non nitrogen fertilization (NNF). Soil physico-chemical properties and growth and yield potential of silage corn were examined. The tested soils showed strong alkali and saline properties with low contents of organic matter and available phosphate while contents of exchangeable sodium and magnesium were high. Soil salinity increased during cultivation of summer crop. However, corn was not affected by salt content. The fresh weight of GMC at incorporation time was 18,345 kg $ha^{-1}$. Content of total nitrogen was 3.09% and the C/N ratio was 12.8 at incorporation time. Fresh and dry matter yield of silage corn were higher in the order of N30% reduction, CF, N50% reduction, N70% reduction, N100% reduction and NNF. Fresh and dry matter yield potential of silage corn for N30% reduction were comparable to those of CF. Bulk density of the soil decreased with incorporation of GMC, while porosity was increased. The soil pH decreased while content of exchangeable calcium, available phosphate, and organic matter increased. Also contents of exchangeable sodium and potassium decreased with incorporation of GMC. The data indicate that incorporation of hairy vetch can improve soil physical and chemical properties and reduce nitrogen fertilizer application especially for alkali saline reclaimed soil such as Saemangeum reclaimed land.

• 한국토양비료학회지
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• 제45권1호
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• pp.98-102
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• 2012

Organic farming has rapidly increased in Gangwon province, but there is a concern about nutrient accumulation and nutrient imbalance in the soil of organic farming. This study was conducted to investigate the impact of continuous application of organic amendments on growth and yield of red pepper and soil characteristics compared with chemical fertilizers application for four years. Treatments of organic amendments including oil cake, rice straw compost, amino acid compost, rice bran compost, and mushroom media (spent substrate) compost resulted in comparable growth and yield of pepper to chemical fertilizers. Organic amendments improved soil physical and chemical characteristics. Especially, rice bran compost and oil cake significantly increased soil organic matter compared with chemical fertilizer application and mushroom media compost and rice straw compost significantly improved soil aggregate stability. On the other hand, available phosphate level in the soil amended with rice bran compost or mushroom media compost was relatively high compared with the other treatments due to relatively high phosphate levels in the composts. It is not easy to adjust nutrient composition in the organic materials. Therefore, the results obtained from the study imply that nutrient imbalance needs to be carefully considered in organic farming without use of chemical fertilizers.

• 상하수도학회지
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• 제30권1호
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• pp.19-31
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• 2016

The Saemangeum watershed is required to manage water pollution effectively but the effect of liquid manure (LM) on soil and water quality in the basin is not clearly identified as yet. This study aims at assessing the effect on soil of a rice field and water quality of water bodies near the rice field during rice-crop time period to find out the effect of LM, the effect of rainfall, and the effect of rice-crop environment on soil and water quality by analyzing data of nitrogen components. As a result of the LM distribution, $NO_3-N$ was much higher than other N components in the entire soil layers and it was accelerated by rainfall right after the LM distribution. Compared to chemical fertilizer (CF), LM was slightly affected but still influenced on the surface water quality. During weak rainfall, low nitrogen concentration in topsoil was resulted as NH3-N decreased and Org-N and $NO_3-N$ increased. $NO_3-N$ concentration in the water of irrigation canals increased with time. During intensive rainfall, $NO_3-N$ and Org-N of the soil were measured highly in the submerged condition, while the water quality of the rice field was lower due to flooding into the irrigation canal as well as the growth of the rice plants. Also, total nitrogen was increased more than 7 times and it showed serious water quality deterioration due to LM and excessive fertilizer distribution, and rainfall during all rice-crop processes. The effect of LM on water quality should be studied consistently to provide critical data while considering weather condition, cropping conditions, soil characteristics, and so on.

• 한국토양비료학회지
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• 제47권6호
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• pp.594-597
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• 2014

Crop yields depend on the limiting factor of crop growth; Liebig law of minimum. Identifying the kind and the necessary amount of the limiting factor is essential to increase crop yield. Although nitrogen is the most essential nutrient, N application does not always bring about yield increases when other elements are limiting in rice cultivation. Two experiments were compared to elucidate the effect of soil testing on rice yield response to N level. The one was an experiment about yield response of 3 rice cultivars to 7 levels of N application, which was conducted from 2003 to 2004 in 25 farmer's fields without ameliorating soil conditions by soil testing and the other was a demonstration experiment on N fertilizer recommendation equation by 0, 0.5, 1.0, and 1.5 times of N recommended level in 5 soil types from 30 fields after ameliorating soil conditions by soil testing. The N response patterns of the experiments conducted without soil testing showed a Mitscherlich pattern in some cultivars and soil types, but did not in the others. The N response patterns of the demonstration experiment showed a Mitscherlich pattern in all soil types. Because these results indicated that N was the minimum nutrient in the demonstration experiment by ameliorating soil conditions with soil testing, but not in the other experiment without soil testing, the supply of minimum nutrients by soil testing could increase the efficiency of N-fertilization.

• 한국토양비료학회지
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• 제48권1호
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• pp.22-29
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• 2015

The objectives of this study were to monitor the changes in soil solution nutrients and to evaluate their effect on rice uptake and yield. The changes of chemical characteristics of paddy soil solution were examined from the 58th fertilization experiment in which the continuous rice cropping experiment started in 1954 at the National Academy of Agricultural Science. The treatments were no fertilization (No fert.), inorganic fertilization (NPK), inorganic fertilizer plus rice straw compost (NPKC) and inorganic fertilizer plus silicate and lime fertilizer as a soil amendment (NPKCLS). The fertilizers were added at rates of standard fertilizer application rate in which nitrogen (N), phosphate ($P_2O_5$), potassium ($K_2O$), and sililcate ($SiO_2$) were applied at rates of $75{\sim}150kg\;ha^{-1}$, $70{\sim}86kg\;ha^{-1}$, $75{\sim}86kg\;ha^{-1}$, and $7.5Mg\;ha^{-1}$ respectively and lime was applied to neutralize soil acidity until 6.5. Average Electrical Conductivity (EC) of soil solution in NPKCLS and NPKC ranged from 1.16 to $2.00dS\;m^{-1}$. The $NH{_4}^+$ and $K^+$ levels in NPKCLS and NPKC were higher than that of the other treatments, due to high supply power of rice straw compost. The content of $H_3SiO{_4}^-$ was higher in NPKCLS because of silicate application. The dominant ions in soil solution were $Ca^{2+}$, $Mg^{2+}$ and $Na^+$ among cations and $HCO{_3}^-$, $SO{_4}^{2-}$, and $Cl^-$ among anions in all treatments. The continuous application of inorganic fertilizers plus rice straw compost (NPKC) and silicate fertilizer (NPKCLS) led to the changes of various chemical composition in soil solutions. Also, they had a significant impact on the improvement of rice inorganic uptake and grain yield. Especially, inorganic uptake by rice in NPKC and NPKCLS significantly increased than those in NPK plot; 14~46% for T-N, 32~36% for P, 43~57% for K, and 45~77% for Si. Therefore, the combined application of inorganic fertilizers with organic compost as a soil amendment is considered as the best fertilization practice in the continuous rice cropping for the improvement of crop productivity and soil fertility.

• 농업과학연구
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• 제50권4호
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• pp.713-721
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• 2023

Soil acidification challenges global food security by adversely influences soil fertility and agricultural productivity. Carbonized agricultural residues present a sustainable and ecofriendly way to recycle agricultural waste and mitigate soil acidification. We evaluated the effects of organic amendments on lettuce growth and soil chemical properties in two soils with different pH levels. Carbonized rice husk was produced at 600℃ for 30 min and rice husk was treated at 1% (w·w^-1). Carbonized rice husk increased soil pH, electrical conductivity, total carbon content, and nitrogen content compared with untreated and rice husk treatments. Furthermore, this study found that lettuce growth positively correlated with soil pH, with increasing soil pH up to pH 6.34 resulting in improved lettuce growth parameters. Statistical correlation analysis also supported the relationship between soil pH and lettuce growth parameters. The study findings showed that the use of carbonized rice husk increased the constituent elements of lettuce, such as carbon, nitrogen, and phosphate content. The potassium content of lettuce followed a similar trend; however, was higher in acidic soil than that in non-acidic soil. Therefore, improving the pH of acidic soil is essential to enhance agricultural productivity. It is considered advantageous to use agricultural residues following pyrolysis to improve soil pH and agricultural productivity.

• 농업과학연구
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• 제50권2호
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• pp.273-281
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• 2023

Ammonia (NH₃) emitted from the use of fertilizers during agricultural practice generates particulate matter and odors. The application of carbonized rice husk, an eco-friendly material, is one of the measures used to reduce NH₃. The objective of this study was to evaluate the effect of the application rate and pH of carbonized rice husk on NH₃ emissions and soil quality. An experiment to assess NH₃ emissions was performed in a glasshouse using a static chamber method. The pH of the carbonized rice husk was divided into acidic, neutral, and basic groups, and the carbonized rice husk application rates were 1, 3, and 5% of the soil weight. NH₃ emissions showed a sharp increase within three days after the inorganic fertilizer was applied. Subsequently, NH₃ emissions decreased rapidly after basal fertilization compared to primary and secondary top-dressing. When carbonized rice husks were applied to soil, NH₃ emissions decreased in all treatments, and neutral carbonized rice husk was the most effective in comparison with acidic and basic carbonized rice husk. The application rate of carbonized rice husk and NH₃ emissions showed a negative correlation, and the lowest emissions were found in units with a 5% application rate. Also, there was no statistically significant difference between NH₃ emissions according to the application rate of carbonized rice husk, and when carbonized rice husks were applied at a 5% rate, soil OM increased excessively. Therefore, it is recommended to apply only 1% neutral carbonized rice husk to most effectively reduce NH₃ emissions in the soil.