• Journal of Plant Biology
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• 제38권4호
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• pp.365-371
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• 1995

Binary vectors, pBI-ActR1, pBI-ActF1 and pBSH-ActR1, were constructed using pGA642, the replication origin of pTi12 and the rice actin promoter. The sizes of pBI-ActR1, pBI-ActF1 and pBSH-ActR1 were 12.9 kb, 13.2 kb and 11.95 kb, respectively. These vectors containing a rice actin promoter followed by a GUS structural gene could induce stronly the expression of GUS gene in transformed rice cells. Rice explants from 3-4 day old seedlings after germinatin were cocultured with A. tumefaceins harboring pBI-ActR1, pBI-ActF1 or pBSH-ActR1, and then GUS expression in the explants was assayed. Transformation of rice explants by these binary vectors was tissue-specific, such that the meristematic regions of shoot apex, root and hypocotyl were transformed by these binary vectors.

• 한국잡초학회지
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• 제10권4호
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• pp.248-254
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• 1990

동일(同一) 포장(圃場)에 벼를 묘령별(苗令別)(8일묘(日苗), 20일묘(日苗), 35일묘(日苗))로 재식(栽植)한 후(後) butachlor, dithiopyr, pyrazolate 등의 각단제(各單劑) (pyrazolate는 8일묘(日苗)에만 사용(使用)) 및 dithiopyr+pyrazolate, dithiopyr+benzulfuron - methyl, butachlor+beansulfuron methyl(20일묘(日苗) 및 35일묘(日苗))에만 사용(使用)등의 제초제(除草劑)를 처리(處理)하여 초기약해(初期藥害) 및 생육(生育), 수량(收量)등을 조사(調査)한 결과(結果)는 다음과 같다. 1. 묘령별(苗令別)로 각(各) 실행구(實行區)의 생육(生育), 수량구성요소수량(收量構成要素收量), 비교조사(比較調査)한 바 유의차(有意差)가 없었다. 단(但) 출수일자(出穗日字)는 8일묘(日苗)가 20일묘(日苗) 보다 2일(日) 늦고 35일묘(日苗) 보다는 6일(日) 늦었다. 2. 각(各) 묘령구(苗令區) 모두 공시제초제(供試除草劑)에 대하여 약해(藥害)는 동일(同一)하게 경미(輕微)하였고, 그후의 초장(草長), 분얼수(分蘖數) 모두 실행구(實行區) 대비(對比)(56 DAT시(時)) 유의차(有意差)가 없었다. 3. 제초효과(除草效果)는 공시제초제(供試除草劑) 모두 우수 하였다. 4. 각(各) 수량구성요소(收量構成要素), 수량(收量) 모두 각(各) 묘령별(苗齡別)의 실행구(實行區)에 비(比)하여 유의차(有意差)가 없었다.

• 한국작물학회지
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• 제36권4호
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• pp.287-293
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• 1991

벼 기계이앙을 위한 어린모 육묘에서 종자 소독시 Metalaxy과 살충제를 혼합하여 소독ㆍ침종할 때 묘생육, 맺트형성, 입길병 및 뜸묘 발생에 미치는 효과를 조사하였던 바 다음과 같은 결과를 얻었다. 1. Metalaxyl(25% 수화제, 1,000배석)＋Prochloraz 종자침종처리는 무처리 (Prochloraz)에 비하여 근수가 많고 근장이 현저히 길어져서 어린모의 맷트형성이 아주 양호하였다. 2. Metalaxyl 종자침종처리는 어린모의 근활력을 높여서 뿌리생장을 왕성하게 하고 지상부의 생리적 활력을 증대시키므로서 입길병과 뜸묘의 발생을 방지하였다. 3. 종자소독제인 Prochloraz와 Metalaxyl 및 살충제를 혼합하여 종자를 소독ㆍ침종하더라도 어린모의 맷트형성이 촉진되었고 입묘병과 뜸묘 발생을 방지하였으며, 묘생육도 양호하였다 4. Metalaxyl 종자침종처리는 무처리에 비하여 뿌리생장을 현저히 촉진시키므로서 어린모맷트의 경도(2.5배)와 장력(1.5배)이 크게 증가되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2023년도 춘계학술대회
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• pp.91-91
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• 2023

• 한국작물학회:학술대회논문집
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• 한국작물학회 2021년도 추계학술대회
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• pp.11-11
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• 2021

• 농업과학연구
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• 제42권1호
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• pp.1-5
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• 2015

Drought is one of the major abiotic constraints limiting quantity and quality of rice grain. In order to elucidate the effects of drought on chemical compositions of rice grain, seedlings were cultivated in a rainout shelter controlled with well-watered or water-deficit conditions. After harvesting, the key components including proximates, amino acids, fatty acids, minerals, and vitamins of rice grains were analyzed. Drought stress increased the amounts of methionine, phenylalanine, linoleic acid, linolenic acid, calcium, potassium, vitamin B1, and vitamin E in rice grains, whereas it decreased the contents of lignoceric acid. Particularly, drought stress caused a marked increase in vitamin E content. These results indicate drought significantly influence the chemical compositions of rice grains.

• The Plant Pathology Journal
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• 제35권3호
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• pp.280-286
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• 2019

In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae-infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent.

• 한국토양비료학회지
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• 제14권3호
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• pp.130-136
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• 1981

Rice hull was reduced to ash by carbonization grades to illcuidate alkalinity increase and extract of inorganic nutrients in the rice hull charcoal. Alkaline reaction of water extraction was neutral at less carbonized charcoal, but much carbonized ash from 65% weight loss reached over 10 of pH value, also origin shape of rice hull was maintained until near 65% carbonized grade. Therefore, physical properties sustained good condition for seedling bed. The more charcoal carbonized to ash, the pH value and concentration of inorganic nutrient in their extracts were increased gradually. Nitric acid concentrations for neutralizing extract from charcoal were stronger in proportion to the carbonized grade but 0.1 N nitric acid solution was very reasonable to neutralize the 65% carbonized charcoal for mixing with heavy texture acidy soil(pH 5.3) of uncultivated deep horizon to transplant the tobacco seedlings. Volume ratio mixing for seedling bed is adequate at five of ash to one of acid solution. Neutralization with nitric acid solution also accelerated extraction of the inorganic nutrient in rice hull ash. Tobacco seedlings grown on bed mixed with neutralized rice hull charcoal and soil had shown better results on the agronomic measurement than alkaline ash bed, and phosphorus and cations were uptaken more amounts.

• 한국균학회지
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• 제12권2호
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• pp.65-73
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• 1984

Fusarium moniforme의 4가지 Strain중 Strain IV가 가장 병원성(病原性)이 강(强)하면서 유묘(幼苗)를 도장시키면서 고사(枯死)시키었다. 이병주(罹病株)는 Strain에 관계없이 밀양 23호와 남풍벼는 mesocotyl를 형성하였으나 삼남벼와 진주벼는 mesocotyl를 형성하지 않았다. Mesocotyl를 형성하는 밀양 23호와 남풍벼는 포장에서 건전화(健全化)되었으나 mesocotyl를 형성하지 않는 삼남벼와 진주벼는 대부분 고사(枯死)하였다. 이병(罹病)된 부위(部位)에서 병원균(病原菌)을 분리(分離)한 결과(結果)가 Strain IV가 가장많이 분리(分離)되었으며 분리부위(分離部位)는 벼전체에서 분리(分離)되었다. 키다리병출현(病出現)은 Strain IV에 이병(罹病)된것이 가장많이 본답(本畓)에서 키다리증상(症狀)을 보였으며 주당(株當)분얼수와 수량도 Strain IV에 이병(罹病)된 것은 상당히 감소 되는 경향이었다.

• Journal of Microbiology and Biotechnology
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• 제26권4호
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• pp.748-756
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• 2016

In the present study, a lipopeptide (named AXLP14) antagonistic to Xanthomonas oryzae pv. oryzae (Xoo) was obtained from the culture supernatant of Bacillus amyloliquefaciens B014. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis demonstrated that AXLP14 consisted of surfactin homologs. The minimum inhibition concentration and minimum bactericidal concentration of AXLP14 against Xoo were determined to be 1.25 and 2.50 mg/ml, respectively. At a concentration of 0.613 mg/ml, AXLP14 strongly inhibited the formation of Xoo biofilm. AXLP14 also inhibited the motility of Xoo in a concentration-dependent manner. Applying AXLP14 to rice seedlings significantly reduced the incidence and severity of disease caused by Xoo. In Xoo-infected rice seedlings, AXLP14 strongly and continuously up-regulated the expression of both OsNPR1 and OsWRKY45. In addition, AXLP14 effectively inhibited the Xoo-induced up-regulation of the expression of the abscisic acid biosynthesis gene OsNECD3 and the abscisic acid signalingresponsive gene OsLip9, indicating that AXLP14 may protect rice against Xoo-induced disease by enhancing salicylic acid defense and interfering with the abscisic acid response to virulence.