• KSBB Journal
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• 제31권4호
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• pp.284-290
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• 2016

In this research, recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) was produced by transgenic rice cells. RAmy3D promoter was used for overcome the limitation of low expression level in transgenic plant cells, and the secretion of target protein was accomplished by signal peptide. However, the RAmy3D promoter system which can be induced only by sugar starvation causes the decrease of cell viability. As a result, cell death promotes the release of protease which degrades the target proteins. The protein stability and productivity can be significantly influenced by proteolysis activity. Therefore, development of new strategies are necessary for the in situ recovery of target proteins from cell culture media. In this study, in situ recovery was performed by various strategies. Direct addition of Protein A resin with nylon bag leads to cell death by increased shear stress and decrease in production of hCTLA4Ig by protease. Medium exchange through modified flask could recover hCTLA4Ig with high cell viability and low protease activity, on the other hand, the productivity was lower than that of control. When in situ recovery was conducted at day 7 after induction in air-lift bioreactor, 1.94-fold of hCTLA4Ig could be recovered compared to control culture without in situ recovery. Consequently, in situ recovery of hCTLA4Ig from transgenic rice cell culture could enhance productivity significantly and prevent degradation of target proteins effectively.

• 한국초지조사료학회지
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• 제34권4호
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• pp.227-233
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• 2014

이 연구는 남부지역 논에서 동계 사료맥류, 하계 조생종 벼, 추계 하파귀리 등 삼모작 재배기술을 확립하기 위해실시하였다. 월동 사료작물을 조생종 벼인 조평벼의 이앙시기에 맞춰 4월 30일 1차 수확한 처리구와 사료맥류의 수확기인 5월 30일에 수확한 처리구 모두 곡우호밀이 각각 생초수량 32.0톤/ha과 42.3톤/ha, 건물수량이 5.8톤/ha과 16.5톤/ha로 다른 작물에 비해 높은 수량을 보였다. 하계 조평벼는 5월 6일에 1차 이앙한 처리구보다 6월 4일에 2차 이앙한 처리구에서 등숙비율이 떨어지고 수량도 약 22% 감소하여 조기 이앙이 유리한 것으로 나타났다. 틈새작물로 재배한 하이스피드 귀리는 8월 28일에 1차 파종한 귀리는 10월 10일에 출수하였지만 9월 2일에 입모중으로 2차 파종한 귀리는 출수도 하지 못하였으며 1차 파종에 비해 건물수량과 TDN 수량 모두 50%를 못 미치는 결과를 나타내어 파종이 빠를수록 유리한 것을 알 수 있었다. 따라서 남부지역에서 삼모작을 하기 위해서는 호밀을 재배하여 4월 30일 전에 수확한 후 조평벼의 조기 이앙과 하이스피드 귀리의 적기 파종이 쌀 수량과 조사료 수량 확보에 유리한 것으로 사료되었다.

• 대한지리학회지
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• 제47권5호
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• pp.641-653
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• 2012

강원 영동지역의 천진호와 쌍호에서 확보한 석호 퇴적물을 분석한 결과, 천진호는 AD 780에, 쌍호는 100 BC에 벼농경이 개시된 것으로 추정된다. 두 지점이 멀지 않은 거리 내에 있고 해안으로 연결되어 있음에도 불구하고 벼농경 시작 시점에 큰 차이가 나타나고 있다. 이는 화분자료를 활용하여 특정지역 벼농경문화의 정확한 전파 시점을 파악하는 것이 쉽지 않음을 시사한다. 강원 영동 지역의 경우, 낮은 농경 생산성으로 말미암아 벼농경 문화가 최초로 전파된 시점과 벼농경이 본격적으로 시작된 시점 간에 시간적인 차이가 있었던 것으로 사료된다. 천진호 및 쌍호 분석 자료 모두에서 벼농경이 본격적으로 시작된 지 약 250년 후부터 농경지시화분들의 중요도가 더욱 커지고 산지의 침식이 빠르게 증가하는 모습이 나타난다. 이는 벼농경의 활성화로 인구가 증가함에 따라 저지대에서 산지로 거주지역의 확대가 나타났고 이로 인해 산림이 영향을 받았음을 시사한다.

• 한국작물학회지
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• 제44권3호
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• pp.221-224
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• 1999

A field experiment was carried out to observe the weed control effects of vetch and to evaluate vetch characteristics on clay loam soil in no-till direct-sown rice-vetch cropping systems. The effects of weed control, forage productivity, and N content of vetch plants were investigated. With the progress of plant growth, density of Chinese milk vetch (Astragalus sinicus L.) gradually decreased, but densities of foxtail and other weeds decreased steeply due to the depression by the over-shaded vetch canopy in a no-till direct-sown rice-vetch cropping paddy field. The vetch density in tillage systems was lower than in notillage cropping systems. Lower vetch density occurred with an increase in foxtail density and other weeds. Weed control effect increased by the progress of vetch growth, which indicated that the vetch canopy over-shaded the weeds. Vetch straw was degraded rapidly submergence after with water at the time of wet sowing of rice. Early harvesting of vetch seed resulted in lower seed germination. To acquire enough seedlings without re-sowing, the harvesting of seed should be delayed at least 28 days after the flowering stage in order to ensure the vetch population is sustainable in a no-till direct-sown rice-vetch cropping system. In order to improve the survival of vetch plants, vetch seeds should germinate from the heading .stage to before the full-ripening stage of rice plants. To enhance the percent of over-wintering survival, vetch seeds should germinate no later than the end of October in southern Korea. The dry weight of vetch plants increased with the progress of vetch growth until the flowering stage but N content decreased for 30 days from before the flowering stage (2.9%) until the ripening stage (1.8%). We concluded that Chinese milk vetch could have an effect on weed control before the flowering stage, sustainability without re-sowing of seed annually, and effective green manure for rice pre-crop in no-till direct-sown rice-vetch relay cropping systems.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.160-160
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• 2017

Drought stress is a major constraint of crop development and productivity. Plants have evolutionally developed several mechanisms at the molecular, cellular, and physiological levels to overcome drought stress. The basic Leucine zipper (bZIP) transcription factor (TF) family members are starting to be concerned about their roles in drought stress responses. In this study, we functionally characterized OsbZIP66, a rice group-E bZIP TF, to be associated with rice drought tolerance mechanisms. Expression of OsbZIP66 was significantly induced upon treatments of rice plants with drought, high salinity, and ABA. These observations and the fact that the OsbZIP66 promoter contains ten ABA-responsive elements suggest that OsbZIP66 is up-regulated by drought stress in an ABA-dependent manner. Overexpression of both OsbZIP66 in a whole plant body and specifically in roots enhanced drought tolerance of rice plants, indicating that the rice drought tolerance positively correlates with the expression levels of OsbZIP66. Thus, our results demonstrated that OsbZIP66 has a potential for use in biotechnological development of high-yielding rice plants under drought conditions.

• 한국약용작물학회지
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• 제18권2호
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• pp.74-78
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• 2010

This experiment was carried out to select suitable organic matter in To-jik nursery (self soil nursery) for complement To-jik nursery's defects that are deterioration of raw material by poor quality of seed ginseng and reduction of the quantity in seed ginseng production. Organic matter used were Yacto, rice bean, defatted rice bran, soybean cake and their mixture. As follows, bulk density in soil physical property by treating organic composts was the greatest in soybean cake and the next was followed by mix, Yacto, defatted rice bran, and rice bran treatment in order. Soil pore space ratio was totally the opposite; that was rice bran the first and followed by defatted rice bran, Yacto, mix and soybean cake treatment. The incidence rate of damping off by treating organic composts was 1.5% in both soybean cake and mix while the others was 1.0%. Emergence time was the same among treatment on April 16 and Emergence rate was the highest at 73% in Yacto. There was no significant differences among treatment in the growth of aboveground part but it was a little better in defatted rice bran treatment. In Yacto treatment, the growth of underground part, total root number per kan, rate of first grade ginseng seedling, and rate of usable ginseng seedling etc. were entirely higher but there was little differences. Using defatted rice bran was slightly lower in productivity compared to Yacto, but the possibility was high as a alternative for Yacto in a view of managing cost down.

• 한국환경복원기술학회지
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• 제12권1호
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• pp.52-66
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• 2009

The increase of rice productivity, the decrease of rice demand, aging farmers, and the market-opening of rice along with the domestic and international conditions changes have been led to the increase of the abandoned rice paddy fields. Such abandoned rice paddy fields have been left in many areas and most of them have become wetlands. The purpose of this study is methodology of selection to the wetland for development in the abandoned rice paddy fields along with surroundings. The selection of habitat suitability index assessment study to conservation and restoration was done through hydrology, wild animals habitation, wetland vegetation, outside disturbance, and natural purification capacity assessment. In addition, the same method was applied to assessment items and standards for both the restoration site selection and the type of restoration. Both assessment items and standards were applied to the sites, drawn on the maps, and overlayer for the comprehensive map, and then the wetland suitability index was applied to the suitable site. The development technique was applied to 10 sites near the Banwol Stream watershed in Gunpo, Gyeonggi province. The selection of conservation, restoration, and the optimal sites can lead to not only the biodiveristy increase in agricultural or semi natural areas but also to the establishment of ecological networks in national level.

• Journal of Biosystems Engineering
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• 제41권4호
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• pp.294-303
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• 2016

Purpose: This study aimed to evaluate the profitability of four selective mechanization systems in rice cultivation. Methods: Field experiments were conducted in the farmers' field during the wet season (June to November) of 2015 in Bangladesh. Mechanization systems were applied to evaluate four different selective levels (treatment) in eleven consequent operations. Seedlings were raised in a traditional seedbed and trays for manual and mechanical transplanting, respectively. Land preparation, irrigation, fertilizer, pesticide, carrying, and threshing and cleaning operations were performed using the same method in all the experimental plots. The mechanical options in the transplanting, weeding, and harvesting operations were changed. The mechanization systems were $S_1$ = hand transplanting + hand weeding + harvesting by sickle, $S_2$ = mechanical transplanting + Bangladesh Rice Research Institute (BRRI) weeder + reaper, $S_3$ = mechanical transplanting + BRRI power weeder + reaper, and $S_4$ = mechanical transplanting + herbicide + reaper. This experiment was performed in a randomized complete block design with four replications. Power tiller, rice transplanter, BRRI weeder, BRRI power weeder, self-propelled reaper, BRRI open drum thresher, and BRRI winnower were used in the respective operations. Accordingly, the techno-economic performances of the different technologies were calculated and compared with those of the traditional system. Results: The mechanically transplanted plot produced 6-10% more yield than the hand transplanted plot because of the use of tender-aged seedlings. Mechanical transplanting reduced 61% labor and 18% cost compared to manual transplanting. The BRRI weeder, BRRI power weeder, and herbicide application reduced 74, 91, and 98% labor, respectively. The latter also saved 72, 63, and 82% cost, respectively, compared to hand weeding. Herbicide application reduced the substantial amount of labor and cost in the weeding operation. Mechanical harvesting also saved 96% labor and 72% cost compared to the traditional method of harvesting using sickle. Selective mechanization saved 15-17% input cost compared to the traditional method of rice cultivation. Conclusions: Mechanical transplanting with the safe use of herbicide and harvesting by reaper is the most cost- and labor-saving operation. The method might be the recommended set of selective mechanization for enhancing productivity.

• 한국토양비료학회지
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• 제44권1호
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• pp.1-7
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• 2011

Application of biochar to soils is proposed as a significant, long-term, sink for atmospheric carbon dioxide in terrestrial ecosystems. In addition to reducing emissions and increasing the sequestration of carbon, production of biochar and its application to soils will contribute improve soil quality and crop productivity. Objectives were i) to evaluate biochar productivity from crop residues using a low-cost field scale mobile pyrolyzer and ii) to evaluate characteristics of feedstocks and biochars from locally collected crop residues. Pyrolysis experiments were performed in a reactor operated at $400-500^{\circ}C$ for 3-4 hours using biomass samples of post-harvest residues of corn (Zea mays L.), cotton (Gossypium spp.), rice (Oryza sativa L.), sorghum (Sorghum bicolor L.) and wheat (Triticum aestivum L.). Feedstocks differed, but average conversion to biochar was 23%. Carbon content of biomass feedstock and biochar samples were 445 g $kg^{-1}$ and 597 g $kg^{-1}$, respectively. Total carbon content of biochar samples was 34% higher than its feedstock samples. Significant increases were found in P, K, Ca, Mg, and micro-nutrients contents between feedstock and biochar samples. Biochar from corn stems and rice hulls can sequester by 60% and 49% of the initial carbon input into biochar respectively when biochar is incorporated into the soils. Pyrolysis conversion of corn and rice residues sequestered significant amounts of carbon as biochar which has further environmental and production benefits when applied to soils. Field experiment with crop residue biochar will be investigated the stability of biochars to show long-term carbon sequestration and environmental influences to the cropping systems.

• 한국환경농학회지
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• 제23권1호
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• pp.7-14
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• 2004

관개수 수질을 고려한 감비가 벼의 생산성과 농업환경에 미치는 영향을 분석한 결과는 다음과 같다. 시험에 사용된 관개수의 질소 인, 칼륨의 평균함량은 각각 6.16, 0.26, 9.37 mg/L 이었고, 유입된 영양염류가 논을 통과하면서 농도가 감소되어 유출수의 성분은 유입된 관개수보다는 낮은 농도를 보였으며, 논 표면수와 침출수의 농도는 시비량과 시비시기에 따라 변화하였다. 토양검정 50% 시비구에서는 질소의 공급량과 소비량간의 차이가 0.14 kg/ha으로 나타나 양분수지면에서 균형을 이룬 반면, 농가관행시비구에서는 그 차이가 95.3 kg/ha로 나타났다. 농가관행시비구에 비해 토양검정시비구와 토양검정 50% 시비구는 등숙률, 천립중, 도정특성 등이 우수하여 완전미수량에서 큰 차이를 보이지 않았으며, 시비량이 적을수록 단백질 함량 또한 적어, 기계적인 식미치가 높게 나타나 품질에서 우수하였다. 시비효율에서는 토양검정 50% 처리에서 시비된 성분이 수량에 기여하는 비율이 높아서 시비이용과 효율면에서 우수한 결과를 보였다. 이상과 같이, 관개수에 포함된 영양물질을 작물의 생육에 이용함으로서, 농업용수의 수자원 이용효율을 높이는 동시에, 비료 절감효과와 생산량 증대의 가능성을 검토할 수 있었다.