• 한국작물학회지
• /
• 제44권1호
• /
• pp.26-31
• /
• 1999

This experiment was conducted to evaluate genetic variation in 48 rice accessions (Oryza sativa L.) using AFLP and RAPD markers. For AFLP, a total of 928 bands were generated with 11 primer combinations and 327 bands (35.2%) of them were polymorphic among 48 accessions. In RAPD analyses using 22 random primers 145 bands were produced, and 121 (83.4%) were polymorphic among 48 accessions. Each accession revealed a distinct fingerprint by two DNA marker systems. Cluster analysis using AFLP-based genetic similarity tended to classify rice cultivars into different groups corresponding to their varietal types and breeding pedigrees, but not using RAPD-based genetic similarity. The AFLP marker system was more sensitive than RAPD in fingerprinting of rice cultivars with narrow genetic diversity.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.331-331
• /
• 2017

This study was conducted to know the influence of air temperature and sunshine duration on yield, chemical component, pigment color difference and starch characteristics of two colored rice cultivars in the plain area of Yeongnam province in Korea. The $L^*$, $a^*$, and $b^*$ value of brown rice in Hongjinju and Josaengheugchal rice cultivars was significantly different at continuous cultivated years, 2015 and 2016. The $L^*$, $b^*$ value of two colored rice was significantly increased in 2016 compared to 2015. The $a^*$ value of Josengheugchal rice cultivar was also significantly higher at 2016 than at 2015. It can be noticed the $a^*$, $b^*$, $L^*$ values in Josaengheugchal varied more than those in Hongjinju. Air temperature during ripening period in 2016 was higher than 2015, especially minimum temperature was too high to proper maturation for rice quality. In Josaengheugchal rice cultivar, sunshine duration after heading was longer in 2016 than in 2015. On the contrary, Hongjinju rice cultivar was ripened under condition of insufficient sunshine duration in 2016. The short growing duration by high temperature and long shiny duration made the lack of pigment for Josaengheugchal brown rice. In Hongjinju rice cultivar, shorten sunshine duration and higher night temperature were the source of the pigment deficiency. The grain size of rice which produced in 2016 was bigger than that of 2015 in both rice cultivars. The 1,000 grain weight of rice from 2016 was also bigger than that of 2015. Head rice ratio was high in the rice cultivars produced in 2015. Protein of milled rice in 2016 was more decreased than that of 2015 in Josaengheugchal rice cultivar, it showed reverse result in Hongjinju rice cultivar. Amylose contents of milled rice in 2016 were more decreased than that of 2015 in Hongjinju rice cultivar. Branch chain length distribution of amylopectin was shown a distinct difference between Josaengheugchal and Hongjinju rice flours by each produced year. Josaengheugchal rice cultivar produced in 2015 had a higher amount of short chains than that of 2016 rice starches. In Josaengheugchal rice cultivar, the pasting temperature and peak, trough, breakdown, final viscosity increased in rice flour which produced at 2016, whereas the setback viscosity and peak time showed lower value than those of rice from 2015. The most pasting properties (except of setback viscosity) of rice starch in Hongjinju rice cultivar grown in 2015 were higher than those of rice cultivar produced in 2016.

• 식물병연구
• /
• 제19권4호
• /
• pp.243-253
• /
• 2013

벼 도열병은 안정적인 쌀 생산을 위협하는 대표적인 병으로, 도열병 방제에는 저항성 품종의 육종이 가장 효과적으로 이용되었다. 병원균의 꾸준한 수집과 이들을 이용한 저항성 검정 테스트는 신품종 육성 프로그램에서 중요한 과정이다. 1985년 이래 우리나라의 도열병균은 8개의 판별품종을 이용한 레이스 판별시스템을 구축하여 분류하였다. 그러나 기존의 판별품종은 유전자형이 연구된 바 없어 새로운 레이스 출현이나 병 저항성 붕괴 등에 대하여 과학적으로 분석하여 신속하게 대처하기가 어려웠다. 따라서 이 연구에서는 유전자형이 밝혀진 단인자 저항성 계통과 우리나라에서 수집된 200개 균주의 병원성 반응을 분석하여, 새로운 판별 품종 시스템의 필요성을 제시하였다. 또한, 24개의 단인자 저항성 계통의 병원성 반응을 통해 9개의 대표 저항성 유전자를 선발하였으며, 이에 따른 10개의 대표균주 그룹과 그 저항성 반응을 인덱스화 하고 30개의 한국형 대표균주를 선발하였다. 이 연구는 향후 도열병 저항성 검정 및 벼 신품종 육종에 기초자료로서 활용될 수 있을 것이다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.330-330
• /
• 2017

The tallest culm length was observed in Geonganghongmi cultivar grown in coast area, and culm length was always taller in coast area than that of inland area among these three red rice cultivars. However, number of tiller per rice plant was not affected by different cultural area. Number of spikelets per panicle was much more increased in inland area. Regional difference in number of spikelets per panicle was only observed in Jeongjinju cultivar. Among these red rice cultivars, the highest brown rice yield was Jeongjinju rice cultivar having 702kg in inland area and 692kg in coast area, respectively. The $L^*$ values, which expresses the brightness, were in the range of 36.3 ~ 36.7, 36.7 ~ 37.0, and 38.7 ~ 39.6 in Jeogjinju, Hongjinju, Geonganghongmi rice cultivars, respectively. The $a^*$ values, which expresses the redness positively and greenness negatively were in the range of 12.3 ~ 12.8, 12.2 ~ 12.3, and 12.1 ~ 11.9 in Jeogjinju, Hongjinju, Geonganghongmi rice cultivars, respectively. In addition, the b values, which expresses the yellowness positively and blueness negatively were in the range of 13.8 ~ 14.0, 13.6 ~ 13.5, and 14.9 ~ 14.7 in Jeogjinju, Hongjinju, Geonganghongmi rice cultivars, respectively. Anthocyanidin content ranged 524 to $610{\mu}g/g$ dry weight basis. Cyanidin content was 11.4 to 14.0 times higher than that of delphinidin under coast and inland area. Among these rice cultivars, anthocyanidin content was always higher in rice cultivar grown at coast area. Highest head brown rice rate was only observed in Geonganghongmi to 95.2 at coast area and 95.4 inland area, respectively. Protein content was always higher in coast area than that of inland area. The lowest protein content was measured in Jeogjinju and amylose content was relatively increased in inland area compared to that of coast area. Consequently, in considered with brown rice yield and pigment content, Hongjinju rice cultivar was recommended in optimal pigment rice cultivar in eastern coast of Gyeongsangbuk-do Province.

• 한국작물학회지
• /
• 제48권2호
• /
• pp.108-112
• /
• 2003

Foehn damage on rice plant is one of the important abiotic stresses in eastern costal area of Korea. To know the relationship between foehn impact and morphological traits of rice, wind tunnel method was used with 106 rice cultivars. Less influencing traits on foehn were short panicle, large panicle number per hill, thin panicle neck, and the flag leaf of narrow, short and thick. Leaf pubescence reduced the impact on foehn. 'Naepungbyeo' belong-ed to foehn tolerant varietal group, while 'Ansanbyeo' belonged to the opposite group. Three levels of root cutting treatment with two rice cultivars were conducted to evaluate the foehn impact using wind tunnel. The severity of wind damages was followed the sigmoidal curve duration of wind tunnel treatment were prolonged. Different responses of root cutting to wind tunnel treatment could be used to evaluate the severity of the foehn impact. 'Naepungbyeo' was one of the less implausible cultivars on foehn. 'Nae-pungbyeo' showed tolerant response to wind under 21% root removing treat-ment (20㎝ root cutting), however 'Ansanbyeo' was wilted under the same treatment. In case of 35 % root removing treatment(10㎝ root cutting), both rice cultivars failed to alive against foehn wind.

• Applied Biological Chemistry
• /
• 제46권2호
• /
• pp.117-122
• /
• 2003

화청찰벼, 화청벼, 그리고 이들의 거대배아미 품종인 화청찰거대배아미, 화청거대배아미 등 4품종의 쌀로부터 전분을 분리하여 몇몇 이화학적 특성을 비교하였다. 거대배아 품종으로 유기되면서 아밀로오스의 함량은 감소하고 있었으며, 아밀로오스 분획의 포도당 사슬의 길이도 짧아지고 있었다. 아밀로펙틴의 미세구조는 거대배아 품종으로 유기됨에 따라 B chain의 사슬길이가 약간씩 증가하는 경향이 있고, A chain의 양은 감소하고 있었으며, 아밀로펙틴을 구성하는 포도당의 평균 사슬길이는 약간씩 증가하고 있었다. X선 회절도상에서는 4품종 모두 전형적인 A type이었으며, glucoamylase에 의한 가수분해도는 찰품종의 경우에는 거대배아미로 유기되면서 가수분해도가 낮아지는데 비해서 메품종의 경우에는 반대의 결과를 얻었다. 호화종료온도는 거대배아미 품종으로 유기됨에 따라 낮아지고 있었고, 호화 엔탈피는 거대배아미 품종으로 유기됨에 따라 증가하고 있었다.

• Plant Breeding and Biotechnology
• /
• 제2권3호
• /
• pp.224-230
• /
• 2014

Amplified fragment length polymorphism (AFLP) is a molecular marker technique based on DNA and is extremely useful in detection of high polymorphism between closely related genotypes like Korean wheat cultivars. Six sequence characterized amplified regions (SCARs) have been developed from inter simple sequence repeat (ISSR) analysis which enabled the identification and differentiation of 13 Korean wheat cultivars from the other cultivars. We used six combinations of primer sets in our AFLP analysis for developing additional cultivar-specific markers in Korean wheat. Fifty-eight of the AFLP bands were isolated from EA-ACG/MA-CAC, EA-AGC/MA-CTG and EA-AGG/MA-CTA primer combinations. Of which 40 bands were selected to design SCAR primer pairs for Korean wheat cultivar identification. Three of 58 amplified primer pairs, KWSM006, KWSM007 and JkSP, enabled wheat cultivar identification. Consequently, 23 of 32 Korean wheat cultivars were classified by eight SCAR marker sets.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.225-225
• /
• 2022

Rice is a major food crop consumed by approximately half of the world's population. Heading date is one of the major agronomic traits and has a wide impact on the productivity and quality of rice. Recently, shortening the growth period of rice through modulating heading date has been emphasized as one of the most effective strategies for reducing methane emissions from paddy fields. OsPRR37, a major heading date gene in rice, represses flowering under both short-day and long-day conditions. Plants carrying the loss-of-function alleles of OsPRR37 have been reported to flower approximately seven days and 20 days earlier than those carrying the functional alleles in short day and long day conditions, respectively. In this study, we investigated the nucleotide sequence variation existing in the exonic regions of OsPRR37 and catalogued the allelic distribution in 208 Korean rice cultivars. We used four sets of primers for amplifying and sequencing the eight exons of OsPRR37. As a result, two types of loss-of-function alleles and four types of functional alleles were found in 208 Korean rice cultivars. Interestingly, only three cultivars (Jinbuolbyeo, Jinseolchal, and Mimyeon) carried loss-of-function alleles while 205 carried functional alleles, indicating that OsPRR37 loss-of-function alleles have been used very rarely in Korean rice breeding programs. To generate useful information for the development of early-maturing rice cultivars, our future work will focus on analyzing the effect of different OsPRR37 alleles on heading date and other major agronomic traits.

• 한국식품영양학회지
• /
• 제30권6호
• /
• pp.1260-1267
• /
• 2017

The properties of rice were studied on five rice cultivars (Ilpumbyeo, Samkwang, Goami-4, Dodamssal, and Thai rice), and employed two kinds of saccharification treatment methods (treatment I : rice shape, treatment II: grinding rice shape). Thai rice showed differences in width and length when compared to other cultivars of rice, and the Goami 4 had the lowest thousand-grain weight. The Goami4 and Dodamssal each showed high contents of amylose and resistant starch, and the water absorption rate was close to maximum at 90 minutes as well as the highest level of Goami 4 at all times. The qualities of highest water-binding capacity, solubility and swelling power was most significant in Thai rice. The lowest hardness level of wet rice resulted in the lowest hydration-related characteristics. High amylose content rice, in particular, showed low sugar content and slightly increased sugar content as the saccharification process improved (treatment II). On the other hand, high amylose cultivars had the same high degree of hardness as boiled rice. From these results, the Dodamssal was found to have the lowest viscosity at all temperatures but highest viscosity during the saccharification process, suggesting it may be successfully implemented as a thickener in rice beverage processing. The purpose of this study was to attempt to provide basic data on the development of rice beverage manufacturing technology, based upon the quality characteristics related to beverage processing of rice cultivars.

• Current Research on Agriculture and Life Sciences
• /
• 제7권
• /
• pp.117-125
• /
• 1989

벼잎선충(線蟲) 방제(防除)를 위(爲)한 볍씨의 온탕침적조건(溫湯浸績條件), 벼품종간(品種間)의 저항성(抵抗性) 정도(程度), 피해경율(被害莖率)과 수량감소(收量減少) 정도(程度) 등을 구명(究明)하기 위(爲)해 시험(試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 볍씨 온탕침법(溫湯浸法)에 의(依)한 선충(線蟲) 방제효과(防除效果)는 $61^{\circ}C$에서 10-15분간(分間) 처리(處理)하였을때 가장 좋았다. 벼잎선충(線蟲)에 대한 품종간(品種間)의 피해경율(被害莖率)은 통일형(統一型) 품종(品種)의 경우 White tip 증상(症狀)이 나타나지 않았고 일본형(日本型) 품종(品種)들은 대부분 White tip 증상(症狀)이 잘 나타났는데 그 중(中)에서도 오태(五台)벼와 부광벼는 각각(各各) 95.6%, 81.8%의 높은 피해경율(被害脛率)을 보였다. 그러나 볍씨내 선충밀도(線蟲密度)에 있어서는 통일형(統一型), 일본형(日本型) 품종(品種) 모두 벼 10g당 100마리 이상(以上) 검출되었다. White tip 증상(症狀)이 나타나지 않았던 통일형(統一型) 품종(品種)들도 선충(線蟲) 접종구(接種區)에서는 수량(收量) 및 수량구성요소(收量構成要素)가 감소(減少)되는 품종(品種)이 많았고 일본형(日本型) 품종(品種)에서는 피해경율(被害莖率)이 높았던 오태(五台)벼와 부광벼는 40% 가까운 수량감소율(收量減少率)을 보였으며 전체적(全體的)으로 통일형(統一型) 보다는 일본형(日本型) 품종(品種)에서 수량구성요소(收量構成要素)와 수량(收量)의 감수정도(減收程度)가 높게 나타났다. 일본형(日本型) 품종(品種) 중(中) 영덕(盈德)5호(號)는 접종구(接種區)에서 White tip 증상(症狀)이 전혀 나타나지 않았고 수량(收量)도 감소(減少)되지 않아 벼잎선충(線蟲)에 대하여 저항성(抵抗性) 품종(品種)으로 생각된다.