• Applied Biological Chemistry
• /
• v.41 no.8
• /
• pp.595-599
• /
• 1998

An analytical method was developed to determine tricyclazole residues in rice grain, straw, and soil using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. Tricyclazole was extracted with methanol from moist rice grain, straw, and soil samples. n-Hexane washing was employed to remove nonpolar co-extractives during liquid-liquid partition. Tricyclazole was then extracted with dichloromethane from alkaline aqueous phase, while acidic interferences remained in the phase. Dichloromethane extract was further purified by silica gel column chromatography prior to HPLC determination. Reverse-phase HPLC using an octadecylsilyl column was successfully applied to separate and quantitate the tricyclazole residue in sample extracts monitored at ${\lambda}_{max}$ 225nm. Recoveries from fortified samples averaged $95.5{\pm}3.0%\;(n=6),\;87.5{\pm}20.%\;(n=6),\;and\;84.3{\pm}2.8%$ (n=12) for rice grain, straw, and soil, respectively. Detection limit of the method was 0.02 mg/kg for rice grain and soil samples while 0.05 mg/kg for rice straw samples. The proposed method was reproducible and sensitive enough to evaluate the safety of tricyclazole residues in rice grain, straw, and soil.

• Korean Journal of Environmental Agriculture
• /
• v.30 no.4
• /
• pp.432-439
• /
• 2011

BACKGROUND: An official analytical method was developed to determine etofenprox residues in agricultural commodities using high-performance liquid chromatography (HPLC). METHODS AND RESULTS: The etofenprox residue was extracted with acetone from representative samples of five raw products which comprised rice grain, apple, mandarin, cabbage, and soybean. The extract was then serially purified by liquid-liquid partition and Florisil column chromatography. For rice and soybean samples, acetonitrile/n-hexane partition was additionally coupled to remove nonpolar lipids. Reversed phase HPLC using an octadecylsilyl column was successfully applied to separate etofenprox from co-extractives. Intact etofenprox was sensitively detected by ultraviolet absorption at 225 nm. Recovery experiment at the quantitation limit validated that the proposed method could apparently determine the etofenprox residue at 0.02 mg/kg. Mean recoveries from five crop samples fortified at three levels in triplicate were in the range of 93.6~106.4%. Relative standard deviations of the analytical method were all less than 10%, irrespective of crop types. A selected-ion monitoring LC/mass spectrometry with positive atmospheric-pressure chemical ionization was also provided to confirm the suspected residue. CONCLUSION(s): The proposed method is simple, rapid and sensitive enough to be employed in routine inspection or monitoring of agricultural products for the etofenprox residue.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.25 no.4
• /
• pp.632-636
• /
• 1996

Soybean and residue contained 59.0% dietary fiber and makkolli residue contained 26.0% dietary fiber. The breads made from flour consisting of 90% wheat flour and 10% fiber materials were compared with that made with 100% wheat flour in terms of baking performance and consumer acceptance. Replacement of 10% wheat flour by soybean curd residue or makkolli residue increased water absorption and loaf weigh, but reduced loaf volume. Sensory panels could not accept favorably the bread made with the soybean curd residue or makkolli residue in flour replacement at 10% level. The protein content was increased from 13.5% to 15.4% in bread containing 10% soybean curd residue and to 16.4% in bread containing makkolli residue. The dietray fiber contents of the bread with soybean curd residue and the bread with makkolli residue were three-fold and two-fold higher than the bread with flour.

• Korean Journal of Environmental Agriculture
• /
• v.18 no.4
• /
• pp.384-387
• /
• 1999

The residue of imidacloprid in hulled rice and paddy soil was investigated. In laboratory conditions, the degradation of imidacloprid in the soils followed first-order reaction kinetic. The rate of degradation was influenced by soil temperature and soil type. The half-life of imidacloprid at $18-28^{\circ}C$ was 66.7-96.3 days in the heavy clay soil and 56.8-117.5 days in the clay loam soil. Arrhenius activation energy obtained from the temperature experiment was 25.5 KJ/mol in heavy clay soil and 50.3 KJ/mol in clay loam soil. In paddy field, the degradation of imidacloprid was fast during the initial period but the degradation rate was gradually slow. About 10 % of the initial amount remained in the soil 120 day after the application. The residual amount of imidacloprid in rice was below the detection limit, 0.01 ppm. The residue level in rice was lower than MRL 0.05ppm in Korea.

• The Korean Journal of Food And Nutrition
• /
• v.10 no.1
• /
• pp.92-96
• /
• 1997

A Korean commercial sweet rice drink "Sikhye" showed sucrose, fructose, glucose, maltose, limit dextrin and various size of maltooligosaccharides in HPLC and TLC analysis. Commercial Sikhye was found to contain 0.09% of limit dextrin and 0.2% of rice residue. Limit dextrin in commercial Sikhye showed both signal of $\alpha$-1,4- and $\alpha$-1,6-glucosidic linkage with its estimation ratio of 15:1 by 1H-NMR analysis. This limit dextrin was hydrolyzed to produce various size of maltooligosaccarides with more longer chain than that of traditional Sikhye by pullulanase. Limit dextrin was digested wit enzymes(30units/ml) of $\alpha$-amylase, $\alpha$-glucosidase and glucoamylase from Aspergillus awamori, sweet potato $\beta$-amylase and human salivary $\alpha$-amylase at 37$^{\circ}C$ for 1 hour, respectively. Hydrolysis rates of these amylases on it were higher than in case of traditional sikhye. $\alpha$-Glucosidase plus human salivary $\alpha$-amylase hydrolyzed it to 61.3%. Hydrolysis rates of these amylases on rice residue were lower than that of traditional Sikye. These results suggest that limit dextrin in commercial Sikhye is less effective than isomaltooligosaccharides in traditional Sikhye as a growth factor for Bifidobacterium while rice residue in commercial Sikhye is more effective than that in traditional Sikhye as dietary fiber.ary fiber.

• Korean Journal of Environmental Agriculture
• /
• v.23 no.4
• /
• pp.251-257
• /
• 2004

Analytical methods were developed to determine cyclosulfamuron residues in soil, water, rice grain and straw using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. In these methods, cyclosulfamuron was extracted with aqueous $Na_2HPO_4$/acetone and acetone/methanol mixture from soil and rice samples respectively. Liquid-liquid partition coupled with ion-associated technique, Florisil column chromatography, and solid-phase extraction (SPE) were used to separate cyclosulfamuron from interfering co-extractives prior to HPLC analysis. For water sample, the residue was enriched in $C_{18}$-SPE cartridge, cleaned up in situ, and directly subjected to HPLC. Reverse-phase HPLC under ion-suppression was successfully applied to determine cyclo-sulfamuron in sample extracts with the detection at its ${\lambda}_{max}$ (254 nm). Recoveries from fortified samples averaged $87.8{\pm}7.1%$ (n=12), $97.3{\pm}7.2%$ (n=12), $90.8{\pm}6.6%$ (n=6), and $78.5{\pm}6.7%$ (n=6) for soil, water, rice grain and straw, respectively. Detection limits of the methods were 0.004 mg/kg, 0.001 mg/L, 0.01 mg/kg and 0.02 mg/kg for soil, water, rice grain and straw samples, respectively.

• Applied Biological Chemistry
• /
• v.37 no.6
• /
• pp.487-491
• /
• 1994

Relative crystallinities, saccharification efficiency, and diazinon(pesticide) residues of rice and rice hull puffed by hot air puffer were investigated. Crystallinities of rice starch in the puffed rice were decreased as compared to those in cooked rice, and those of rice hull were slightly decreased as compared to those in raw hull. By puffing of glutinous rice, the saccharification efficiency treated with malt amylase was higher than in cooked rice, and hydrolysis ratio of rice hull by cellulase was also increased from 14% to 30% by puffing. The residual contents of diazinon of the puffed rice and rice hull were decreased to 42.7% and 47.6%, respectively. In addition, its decreasing rate in nonglutinous rice were higher than that of glutinous rice.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.42 no.5
• /
• pp.564-570
• /
• 1997

Field experiments were carried out to investigate the effects of different residue treatment of vetch sward on soil improvement, rice growth and grain yield from 1995 to 1996. With Chinese milkvetch, pH, Ca, and Mg of paddy soil were increased in subsoil(10~20cm soil depth), and organic matter, P$_2$ $O_{5}$, and K were increased in top soil (0~10cm soil depth). Redox potential of the paddy soil with milkvetch residue was severely reduced at earlier flooding period, and recovered gradually as rice growth continued. Weeds were more abundant in the paddy with unchanged vetch stand. The most dominant weed species in the paddy soil with vetch sward residue was Echinochloa crus-galli, followed by Leersia japonica, Polygonum hydropiper in the order of abundance. Seedling establishment ratio of rice directly sown over vetch-sward was lower than in conventional tillage paddy(no-vetch, tilled) condition. Rice growth pattern, however, was not significantly affected by vetch sward treatments. The number of tillers per square meter and plant height at heading date were not significantly different among the vetch-sward treatments. Grain yield was the highest in plough of vetch vegetation, followed by conventional (no-vetch, tilled), live-mulching of vetch, vetch-removed, and vetch-desiccated by weedcide. The whole grain rice yield was the lowest in vetch-desiccated by weedcide.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.9
• /
• pp.2671-2674
• /
• 2013

To elucidate the role of the evolutionarily conserved Tyr8 residue in rice Phi-class GSTF3, this amino acid was replaced with alanine and phenylalanine by site-directed mutagenesis, respectively. The replacement of Tyr8 with Ala significantly affected the catalytic activity and the kinetic parameters, whereas the substitutions of Tyr8 with Phe had almost no effect. The Y8A mutant resulted in approximately 90-100% decrease of the specific activity. Moreover, the Y8A mutant resulted approximately in 2-fold increase of $K_m$, approximately 60-80% decrease of $k_{cat}$, and approximately 6.5-fold decrease in $k_{cat}/K_m$. From the pH/log $k_{cat}/K_m$ plot, $pK_a$ values of the GSH in the wild-type enzyme-GSH complex, Y8A-GSH complex and Y8F-GSH complex were estimated to be approximately 6.8, 8.5 and 6.9, respectively. From these results, we suggest that the evolutionarily conserved Tyr8 residue in OsGSTF3 seems to influence the structural stability of the active site of OsGSTF3 rather than directly its catalytic activity.

• Korean Journal of Soil Science and Fertilizer
• /
• v.47 no.6
• /
• pp.553-557
• /
• 2014

The steady increase in livestock industry has greatly required the stable production of food and forage crops. As an alternative, rice-forage cropping system has been attempted in several southern areas. The present study was performed to understand whether an application of cattle-manure compost affects soil chemical properties and crop productivity in rice-forage cropping system, rice ${\rightarrow}$ summer oat ${\rightarrow}$ rye, in Jangheong county, south Jeolla province from 2013 to 2014. Treatments was composed of control (no compost), CM1 (compost application before rice transplanting), and CM2 (two-times compost application, before rice transplanting and after rice harvest), and inorganic fertilizers (N, P, and K) were equally dressed in all plots. Yields of rice were not significantly different between treatments, however, oat production was 1.25-fold higher in CM1 and CM2. Nutrient uptake amounts of rye were higher in CM2 than CM1 and control. Total nitrogen in soil was maintained stable level during crop cultivation. And soil organic matter contents in all treatments were increased by crop residue. Available P_2O_5$ and exchangeable K were increased by cattle manure application. Therefore, it suggested that the amount of nutrient by forage crop residue should be considered in rice-forage multiple cultivation.